Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 37,558 G→A 51.2% intergenic (+254/‑255) USA300HOU_0028 → / → ugpQ1 hypothetical protein/glycerophosphodiester phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073037,5580GA51.2% 37.6 / 113.0 78intergenic (+254/‑255)USA300HOU_0028/ugpQ1hypothetical protein/glycerophosphodiester phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base G (22/16);  new base A (16/24);  total (38/40)
Fisher's exact test for biased strand distribution p-value = 1.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.15e-01

ATGCAAAATTGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTCCATAATCAATAC  >  CP000730/37419‑37695
                                                                                                                                           |                                                                                                                                         
aTGCAAAATTGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAggg                                                                                                                                          >  1:153145/1‑141 (MQ=255)
aTGCAAAATTGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAggg                                                                                                                                          >  1:672272/1‑141 (MQ=255)
  gCAAAATTGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAAGTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTAAAAATCAGTTCTCAGTAAAATTGCTGATAAggggg                                                                                                                                        >  2:763832/1‑140 (MQ=255)
   cAAAATTGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTa                                                                                                                                       >  1:625281/1‑141 (MQ=255)
       aTTGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTacaga                                                                                                                                   >  1:486021/1‑137 (MQ=255)
       aTTGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTacaga                                                                                                                                   <  1:479232/141‑5 (MQ=255)
       aTTGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATGGCGGATAAGGGGTacaga                                                                                                                                   >  2:353603/1‑137 (MQ=255)
       aTTGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGAAGATAAGAGTAGCGTTAAAAGCCGTCCTCAATAAAATTGCGGATAAgggagaaggt                                                                                                                                   >  2:1079534/1‑134 (MQ=18)
        ttGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAGAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATGGCTGATAAGGGGTacagaa                                                                                                                                  >  1:6247/1‑136 (MQ=255)
        ttGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAgcggaacagaa                                                                                                                                  >  1:5942/1‑134 (MQ=255)
        ttGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTacagaa                                                                                                                                  >  1:1121189/1‑136 (MQ=255)
         tGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTacagcaa                                                                                                                                 >  2:388079/1‑135 (MQ=255)
         tGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTacagaaa                                                                                                                                 >  1:350345/1‑135 (MQ=255)
           cagagaaGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTacagaaaaa                                                                                                                               <  1:544203/136‑9 (MQ=255)
           cTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGCGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTacagaaaaa                                                                                                                               <  2:1088932/141‑9 (MQ=255)
           cTGATAAAGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAATGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTacagaaaaa                                                                                                                               <  2:858051/141‑9 (MQ=255)
            tGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAgggggacagaaaaac                                                                                                                              >  2:920047/1‑130 (MQ=255)
             gATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGAAGTTCTGGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGAGAAGGGGCAAGTTAAAAga                                                                                                                             >  2:1131275/1‑140 (MQ=255)
                aaGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCGGATAAGGGGTacagaaaaactaga                                                                                                                          >  1:1110228/1‑128 (MQ=18)
                   gggTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATCAGCGGTGCGCGAAAACCATGTCGCAGGAAAATTGCTGATAAGGGGTAAGTTAATAGCAATcaa                                                                                                                       >  2:542855/1‑139 (MQ=18)
                       aaGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGAACGTTGAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGt                                                                                                                   <  1:924110/141‑1 (MQ=255)
                              aaGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTg                                                                                                            >  1:1085062/1‑141 (MQ=255)
                               aGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGc                                                                                                           <  2:148679/141‑1 (MQ=255)
                                               aTTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGa                                                                                           <  2:871941/141‑1 (MQ=255)
                                                  gCAGATAAGAGGTACGTTACAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAACATTGCAGATAAGGGGTACAGaaaa                                                                                        <  1:763832/141‑1 (MQ=255)
                                                       tgagagGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAg                                                                                   <  1:339767/139‑1 (MQ=255)
                                                                 gTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTAc                                                                         >  2:443662/1‑141 (MQ=255)
                                                                       aGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATGGAAGATAAGGGGTACAGAAAAAATAGACTTGATTACAAAATg                                                                   >  2:703843/1‑141 (MQ=255)
                                                                          aGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAg                                                                >  1:196633/1‑141 (MQ=255)
                                                                           gTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGc                                                               <  1:961040/141‑1 (MQ=255)
                                                                               tAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTgg                                                           >  2:831223/1‑141 (MQ=255)
                                                                                     aaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGAACATa                                                     >  2:464066/1‑141 (MQ=255)
                                                                                           cAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGAt                                               <  1:1063185/141‑1 (MQ=255)
                                                                                           cAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGAt                                               <  1:1063025/141‑1 (MQ=255)
                                                                                            aGATAAGAGGTTTGTTAAAAGCAGTTCTAAGTAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGAtt                                              >  2:652303/1‑141 (MQ=17)
                                                                                             gATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGAttt                                             <  1:767122/141‑1 (MQ=11)
                                                                                               tAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGAttttt                                           >  2:1048001/1‑141 (MQ=14)
                                                                                                     gtacGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTaaaaa                                     <  1:532332/138‑1 (MQ=14)
                                                                                                     gtacGTTAAAAGCAATTATAAGAAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTaaaaa                                     <  1:564560/138‑1 (MQ=17)
                                                                                                      tacGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAat                                    <  1:994197/139‑1 (MQ=14)
                                                                                                      tacGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAat                                    <  1:614590/139‑1 (MQ=14)
                                                                                                      tacGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAat                                    <  2:175433/139‑1 (MQ=14)
                                                                                                      tacGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCCGATAAGGCGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAat                                    >  2:302561/3‑141 (MQ=17)
                                                                                                      aacGTTAAAAGCAGTTCTATGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTAAGTAAAATTGCTGATAATGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAat                                    <  2:128871/139‑1 (MQ=18)
                                                                                                        cGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAatga                                  <  2:618485/141‑1 (MQ=17)
                                                                                                        cGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAAGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAACACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAatga                                  <  1:43204/141‑1 (MQ=17)
                                                                                                             aaaGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAatgagatga                             <  1:669179/141‑1 (MQ=17)
                                                                                                              aaGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTGAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTCAAAAatgagatgag                            <  2:817374/141‑1 (MQ=255)
                                                                                                              aaGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCGTGGGACATAAATGATTTTTTAAAAatgagatgag                            >  2:1125588/1‑141 (MQ=17)
                                                                                                                gcagtcctaggcaaaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGAc                          <  1:391338/129‑1 (MQ=18)
                                                                                                                gCAGTTCTCAGTAAAATTGAGGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAACATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGATCTTGGGACATAAATGATTTTTTAAAAAtgagatgagag                          >  1:777342/1‑140 (MQ=255)
                                                                                                                gCAGTTCTCAGTAAAANTGCTGATAAAGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGGTTGGGACATAAATGATTTTTTAAAAATGAGATGAGAc                          <  1:1079534/141‑1 (MQ=255)
                                                                                                                gCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGAc                          <  2:1066551/141‑1 (MQ=17)
                                                                                                                gCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGAc                          <  1:283797/141‑1 (MQ=18)
                                                                                                                gCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGAc                          <  2:765921/141‑1 (MQ=17)
                                                                                                                gCAGTTCTAGGAAAAATTACAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGAAAAAATGCAGATAAGGGGTACAGAAAAACTAGACTTGATGACAAAATCGAGCTTGGGACATAAATAAATAAAAAAAAATGAGATGAGAc                          <  2:336980/141‑1 (MQ=9)
                                                                                                                 caattctaggcaaaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACg                         <  2:413388/130‑1 (MQ=18)
                                                                                                                 cAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACg                         <  1:1137994/141‑1 (MQ=255)
                                                                                                                  aGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGt                        <  1:232150/141‑1 (MQ=17)
                                                                                                                    ttctaggcaaaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAACATGGAGCTTGGGACATAAATGAGTTTTTAAAAAAGAGATGAGACGTAg                      >  1:575860/9‑141 (MQ=17)
                                                                                                                    ttctaggcaaaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATGTTTTAAAAATGAGATGAGACGTAg                      >  1:537995/9‑141 (MQ=17)
                                                                                                                    ttCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAg                      <  1:1031214/141‑1 (MQ=255)
                                                                                                                       taggcaaaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGAtt                   >  2:1140313/6‑141 (MQ=18)
                                                                                                                       taggcaaaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGAtt                   >  2:78663/6‑141 (MQ=18)
                                                                                                                       taggcaaaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGAtt                   <  1:701528/136‑1 (MQ=18)
                                                                                                                        cAGTAAAATTGCTGATAAGGGGTAAGCAAAAAGCAAGCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTa                  >  1:576908/1‑141 (MQ=255)
                                                                                                                        aggcaaaaTTGCAGATAAGAGGTGCGTTACAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTGTTTAAAAATGAGATGAGACGTAGATTa                  >  2:871345/5‑141 (MQ=21)
                                                                                                                         ggcaaaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTaa                 <  2:350345/138‑1 (MQ=21)
                                                                                                                         ggcaaaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTaa                 >  1:503498/4‑141 (MQ=21)
                                                                                                                         ggcaaaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTaa                 >  1:33476/4‑141 (MQ=21)
                                                                                                                         ggcaaaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTACGTCCCATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTaa                 <  1:388079/138‑1 (MQ=25)
                                                                                                                         aGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGCTGAGACGTGGATTaa                 >  1:563831/1‑141 (MQ=255)
                                                                                                                          gcaaaaTTGCAGATAAGAGGTGCGTTAAACGCCGTTCTCAGTAAAAGTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAAc                <  1:513331/139‑1 (MQ=21)
                                                                                                                          gcaaaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACTGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAAc                <  1:806925/139‑1 (MQ=21)
                                                                                                                          gaaaaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAAc                <  2:83345/139‑1 (MQ=21)
                                                                                                                             aaaTTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTcc             >  1:993152/1‑141 (MQ=255)
                                                                                                                             aaaTTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTcc             >  2:754367/1‑141 (MQ=21)
                                                                                                                                ttGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTCCATa          >  1:944926/1‑141 (MQ=25)
                                                                                                                                   cagATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTCCATAATc       >  1:410778/3‑141 (MQ=25)
                                                                                                                                    agATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTAGAAAGTCATCCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTCCATAATCa      >  1:567847/2‑141 (MQ=21)
                                                                                                                                    agATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTCCATAATCa      >  1:462897/2‑141 (MQ=25)
                                                                                                                                     gATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTCCATAATCaa     <  1:781819/141‑1 (MQ=25)
                                                                                                                                        aaGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATGAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTACATAATCAATaa  >  2:683203/1‑140 (MQ=255)
                                                                                                                                        aaGGGGTAAGTTAAAAGCAATACTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTCCATAATCAATAc  >  1:622730/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                         
ATGCAAAATTGCTGATAAGGGGTAAGTTAAAAGCAGTTCTCAGTAAAATTGCAGATAAGAGGTACGTTAAAAGCAGTTCTAGGCAAAATTGCAGATAAGAGGTGCGTTAAAAGCAGTTCTCAGTAAAATTGCTGATAAGGGGTAAGTTAAAAGCAATCCTAAGTAAAATTGCAGATAAGGGGTACAGAAAAACTAGACTTGATTACAAAATGGAGCTTGGGACATAAATGATTTTTTAAAAATGAGATGAGACGTAGATTAACTCCATAATCAATAC  >  CP000730/37419‑37695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: