Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,105,002 A→T 6.5% L70* (TTA→TAA)  USA300HOU_1967 ← hypothetical bacteriophage protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,105,0020AT 6.5% 310.9 / 2.1 115L70* (TTA→TAA) USA300HOU_1967hypothetical bacteriophage protein
Reads supporting (aligned to +/- strand):  ref base A (56/48);  new base T (7/2);  total (64/51)
Fisher's exact test for biased strand distribution p-value = 2.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 116 total aligned reads displayed.

TAAGTACTCATCCTCTGAATTATGGTCAATCTTTTCAAGTGATTTAAATTTGACAAGCAAATCATCAATCGTCATTGTCTTCACCATTCAATAAGTCGACGATTTCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAGTTCTAATAAT‑TCTTGTTTTGTCAGCTTATCTAAAGGTACGATATAAACTTTGTCGTACTTATTTTTGATTTGATTTGTCAACAATTCAACACGAGGATTGTTATACCCTTCAGCTGGATACAACTC  >  CP000730/2104862‑2105141
                                                                                                                                             |                                                                                                                                            
tAAGTACTCATCCTCTGAATTATGGTCAATCTTTTCAAGTGATTTAAATTTGACACGCAAATCATCAATCGTCATTGTCTTCACCATTCAATAAGTCGACGATTTCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTa                                                                                                                                              >  1:57068/1‑141 (MQ=255)
   ggaCTCATCCTCTGAATTATGGTCAATCTTTTCAAGTGATTTAAATTTGACAAGCAAATCATCAATCGTCATTGTCTTCACCATTCAATAAGTCGACGATTTCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAAtt                                                                                                                                           >  2:758737/3‑140 (MQ=255)
   gTACTCATCCTCTGAATTATGGTCAATCTTTTCAAGTGATTTAAATTTGACAAGCAAATCATCAATCGTCATTGTCTTCACCATTCAATAAGTCGACGATTTCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATg                                                                                                                                           <  2:984625/141‑1 (MQ=255)
   gTACTCATCCTCTGAATTATGGTCAATCTTTTCAAGTGATTTAAATTTGACAAGCAAATCATCAATCGTCATTGTCTTCACCATTCAATAAGTCGACGATTTCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATg                                                                                                                                           >  2:1052610/1‑141 (MQ=255)
       tCATCCTCTGAATTATGGTCAATCTTTTCAAGTGATTTAAATTTGACAAGCAAATCATCAATCGTCATTGTCTTCACCATTCAATAAGTCGACGATTTCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTc                                                                                                                                       <  2:222743/141‑1 (MQ=255)
         ctCCTTTTAATTATGGTCAATCTTTTCAAGTGATTTAAATTTGACAAGCAAATCATCAATCGTCATTGTCTTCACCATTCAATAAGTCGACGATTTCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGc                                                                                                                                     <  1:515267/140‑1 (MQ=255)
         aTCCTCTGAATTATGGTCAATCTTTTCAAGTGATTTAAATTTGACAAGCAAATCATCAATCGTCATTGTCTTCACCATTCAATAAGTCGACGATTTCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTATTGATTCGc                                                                                                                                     >  2:1100370/1‑141 (MQ=255)
           ccTCTGAATTATGGTCAATCTTTTCAAGTGATTTAAATTTGACAAGCAACTCATCAATCGTCATTGTCTTCACCATTCAATAAGTCGACGATTTCTCTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCAt                                                                                                                                   <  1:559392/141‑1 (MQ=255)
           ccTCTGAATTATGGTCAATCTTTTCAAGTGATTTAAATTTGACAAGCAAATCATCAATCGTCATTGTCTTCACCATTCAATAAGTCGACGATTTCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCAt                                                                                                                                   <  1:1174313/141‑1 (MQ=255)
            ctctGAATTATGGTCAATCTTTTCAAGTGCTTTAAATTTGACAAGCAAATCATCAATCGTCTTTGTCTTCACCATTCAATAAGTCGACGATTTCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGAttgcgatt                                                                                                                                  >  2:101388/1‑135 (MQ=255)
             tctGAATTATGGTCAATCTTTTCAAGTGATTTAAATTTGACAAGCAAATCATCAATCGTCATTGTCTTCACCATTCAATAAGTCGACGATTTCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAg                                                                                                                                 >  1:170435/1‑141 (MQ=255)
              ctaaaTTATGGCCAATCTTTTCAAGTGATTTAAATTTGACAAGCAAATCATCAATCGTCATTGTCTTCACCATTCAATAAGTCGACGATTTCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAGt                                                                                                                                <  2:740912/138‑1 (MQ=255)
               tGAATTATGGTCAATCTTTTCAAGTGATTTAAATTTGACAAGCAAATCATCAATCGTCATTGTCTTCACCATTCAATAAGTCGACGATTTCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAGtt                                                                                                                               <  1:440038/141‑1 (MQ=255)
                gAATTATGGTCAATCTTTTCTAGTGATTTAAATTTGACAAGCAAATCATCAATCGTCATTGTCTTCACCATTCAATAAGTCGACGATTTCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAGttt                                                                                                                              >  1:727908/1‑140 (MQ=255)
                 aaTTATGGTCAATCTTTTCAAGTGATTTAAATTTGACAAGCAAATCATCAATCGTCATTGTCTTCACCATTCAATAAGTCGACGATTTCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAGTTCt                                                                                                                             <  1:186462/141‑1 (MQ=255)
                  aTTATGGTCAATCTTTTCAAGTGATTTAAATTTGACAAGCAAATCATCAATCGTCGTTGTGTTCACCATTCAATGAGTCGACGATTTCACTTTTGACCTTTGGACGAGACGC‑TTTTTTTTGTAATGATTCGCATGGTTCta                                                                                                                            >  1:1181978/1‑141 (MQ=255)
                   ttATGGTCAATCTTTTCAAGTGATTTAAATTTGACAAGCAAATCATCAATCGTCATTGTCTTCACCATTCAATAAGTCGACGATTTCACTTTTAACCATTGAACTAGACTC‑TTTTTTTTGTAATGATTTGCATAGTTCtaa                                                                                                                           >  2:1140037/1‑141 (MQ=255)
                   ttATGGTCAATCTTTTCAAGTGATTTAAATTTGACAAGCAAATCATCAATCGTCATTGTCTTCACCATTCAATAAGTCGACGATTTCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTTTAATGATTCGCATAGTTCtaa                                                                                                                           >  1:968645/1‑141 (MQ=255)
                     aTGGTCAATCTTTTCAAGTGATTTAAATTTGACAAGCAAATCATCAATCGTCATTGTCTTCACCATTCAATAAGTCGACGATTTCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGTTTCGCATAGTTCtaata                                                                                                                         >  2:756450/1‑141 (MQ=255)
                     aTGGTCAATCTTTTCAAGTGATTTAAATTTGACAAGCAAATCATCAATCGTCATTGTCTTCACCATTCAATAAGTCGACGATTTCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAGTGCtatta                                                                                                                         >  2:756530/1‑141 (MQ=255)
                      tGGTCAATCTTTTCCAGTGATTTTAATTTGACAAGCAAATCATCAATCGTCATTGTCTTCACCATTCAATAAGTCGACGATTTCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGTTCTGCATAGTTcaactta                                                                                                                        >  2:258278/1‑135 (MQ=255)
                      tGGTCAATCTTTTCAAGTGATTTAAATTTGACAAGCAAATCATCAATCGTCATTGTCTTCACCATTCAATAAGTCGACGATTTCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAGTTCtaataa                                                                                                                        >  1:527976/1‑141 (MQ=255)
                      tGGTCAATCTTTTCAAGTGATTTAAATTTGACAAGCAAATCATCAATCGTCATTGTCTTCACCATTCAATAAGTCGACGATTTCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTAGCATCGTTCttataa                                                                                                                        >  2:764067/1‑141 (MQ=255)
                       ggTCAATCTTTTCAAGTGATTTAAATTTGACAAGCAAATCATCAATCGTCATTGTCTTCACCATTCAATAAGTCGACGATTTCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAGTTCTaataat                                                                                                                       <  2:443550/141‑1 (MQ=255)
                        gTCAATCTTTTAAAGTGATTTAAATTTGACAAGCAAATCATCAATCGTCATTGTCTTCACCATTCAATAAGTCGACGATTTCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAGTTCTAATAAt‑t                                                                                                                     <  1:967968/141‑1 (MQ=255)
                         tCAATCTTTTCAAGTGATTTAAATTTGACAAGCAAATCATCAATCGTCATTGTCTTCACCATTCAATAAGTCGACGATTTCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAGTTCTAATAAT‑Tc                                                                                                                    <  2:1165429/141‑1 (MQ=255)
                          cAATCTTTTCAAGTGATTCAAATATGACAAGCAAATCATCAATCGTCATTGTCTTCACCATTCAATAAGTCGACGATTTCACTTTTAACCATTGAACTAGACGCTTTTTTTTTGTAATGATTCGCCTAGTTCTAATAAt‑tt                                                                                                                    >  1:286251/1‑140 (MQ=255)
                            aTCTTTTCAAGTGATTTAAATTTGACAAGCAAATCATCAATCGTCATTGTCTTCACCATTCAATAAGTCGACGATTTCACTGTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAGTTCTAATAAT‑TCTTg                                                                                                                 <  1:165337/141‑1 (MQ=255)
                              cTTTTCAAGTGATTTAAATTTGACAAGCAAATCATCAATCGTCATTGTCTTTACCATTGAATAAGTCGACGATTTCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAGTTCTAATAAT‑TCTTGtt                                                                                                               <  2:791591/141‑1 (MQ=255)
                              cTTTTCAAGTGATTTAAATTTGACAAGCAAATCATCAATCGTCATTGTCTTCACCATTCAATAAGTCGACGATTTCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAGTGCTACTAAT‑TCTGGtt                                                                                                               >  2:764053/1‑141 (MQ=255)
                                 tccAAGTGATCTAAATTTGACAAGCAAACCACCAATCGTCATTGTCTTCACCATTCAATAAGTTGACGATTTCACTTTTATCCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAGTTCTAATAAT‑TCTTGTTTTg                                                                                                            <  2:392904/139‑1 (MQ=255)
                                    aaGTGATTTAAATTTGTCAAGCAAATCATCAATCGTCATTGTCTTCACCATTCAATAAGTCGACGATTTCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAGTTCTCATAAT‑TCTTCTTTTGTcc                                                                                                         >  1:374007/1‑140 (MQ=255)
                                          tttAAATTTAACAATCAAATCATCAATCGTCATTGTCTTCACCATTCAATAAGTCGACGATTTCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAGTTCTAATAAT‑TCTTGTTTTGTCAGCTTAt                                                                                                   <  1:500251/141‑1 (MQ=255)
                                           ttAAATTTGACAAGCAAATCATCAATCGTCATTGTCTTCACCATTCAATAAGTCGACGATTTCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAGTTCTAATAAT‑TCTTGTTTTGTCAGTTTAtt                                                                                                  >  1:687350/1‑140 (MQ=255)
                                            tAAATTTGACAAGCAAATCATCAATCGTCATTGTCTTCACCATTCAATAAGTCGACGATTTCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAGTTCTAATAAT‑TCTTGTTTTGTCAGCTTATCt                                                                                                 <  2:1181978/141‑1 (MQ=255)
                                             aaaTTTGACAAGCAAATCATCAATCGTCATTGTCTTCACCATTCAATAAGTCGACGATTTCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCAGAGTTCTAATAAT‑TCTTGTTTTGTCAGCTTATCTa                                                                                                <  2:97098/141‑1 (MQ=255)
                                                      aaGCAAATCATCAATCGTCATTGTCTTCACCATTCAATAAGTCGACGTTTTCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAGTTCTAATAAT‑TCTTGTTTTGTCAGCTTATCTAAAGGTGCGa                                                                                       >  1:40221/1‑141 (MQ=255)
                                                       aGCAAATCATCAATCGTCATTGTCTTCACCATTCAATAAGTCGACGATTTCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAGTTCTAATAAT‑TCTTGTTTTGTCAGCTTATCTAAAGGTACGat                                                                                      <  1:1031405/141‑1 (MQ=255)
                                                       aGCAAATCATCAATCGTCATTGTCTTCACCATTCAATAAGTCGACGATTTCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAGTTCTAATAAT‑TCTTGTTTTGTCAGCTTATCTAAAGGTACGat                                                                                      <  1:1076721/141‑1 (MQ=255)
                                                            atcatcAATCGTCATTGTCTTAACCATTCAATAAGTCGACGATTTCACTTTTAACCATTTAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAGTTCTAATAAT‑TCTTGTTTTGTCGGCTTATCTAAAGGTACGATATaaa                                                                                 >  2:528371/1‑141 (MQ=255)
                                                             tcatcaATCGTCATTGTCTTTACTATTCAATAAGGCGACGATTTCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAGTTCTAATAAT‑TCTTGTTTTGTCAGCTTATCTAAAGGTACGATATAAAc                                                                                <  1:991/141‑1 (MQ=255)
                                                               atcaATCGTCATTGTCTTCACCATTCAATAATTCGACGATTTCGAGCGTAACCATTGAACTAGACGC‑TGTTTTTTGTAATGATTCGCATAGTTTTAATAAT‑TCTTGTTTTGTCAGCTTCTCTAAAGGTACGATCTAAACtt                                                                              >  1:630466/1‑141 (MQ=255)
                                                               atcaATCGTCATTGTCTTCACCATTCAATAAGTCGACGATATAACTTTTAAACGGGGAGCTGGCAGC‑TTTTTTTAGTAATGATTCGCATAGTAGT‑ATAATATCTTGTTTTGTAAGCTTATCTAAAGGTACGATGTAAACtt                                                                              >  1:572154/1‑141 (MQ=255)
                                                                    tCGTCATTGTCTTCACCATTCAATAAGTCGACGATTTCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAGTTCTAATAAT‑TCTTGTTTTGTCAGCTTATCTAAAGGTACGATATAAACTTTGTCg                                                                         <  1:156135/141‑1 (MQ=255)
                                                                       tCATTGTCTTCACCATTCAATAAGTCGACGATTTCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAGTTCTAATAAT‑TCTTGTTTTGTCAGCTTATCTAAAGGTACGATATAAACTTTGTCGtct                                                                      >  1:590621/1‑139 (MQ=255)
                                                                        cATTGTCTTCACCTTTCAATAAGTCGACGATTTCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAGTTCTAATAAT‑TCTTGTTTTGTCAGCTTATCTAAAGGTACGATATAAACTTTGTCGTACt                                                                     <  2:235157/141‑1 (MQ=255)
                                                                        cATTGTCTTCACCATTCAATAAGTCGACGATTTCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAGTTCTAATAAT‑TCTTGTTTTGTCAGCTTATCTAAAGGTACGATATAAACTTTGTCGTACt                                                                     <  2:25583/141‑1 (MQ=255)
                                                                         aTTGTCTTCACCTTTCCATTAGTCGACGATTTCATTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAGTTCTAATAAT‑TCTTGTTTTGTCAGCTTATCTAAAGGTACGATATAAACTTTGTCGTACtt                                                                    <  2:233264/141‑1 (MQ=255)
                                                                         aTTGTCTTCACCATTCAATAAGTCGACGATTTCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAGTTCTAATAAT‑TCTTGTTTTGTCAGCTTATCTAAAGGTACGATATAAACTTTGTCGTACtt                                                                    >  1:1007446/1‑141 (MQ=255)
                                                                          ttGTCTTCACCATTCAATAAGTCGACGATTTCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAGTTCTAATAAT‑TCTTGTTTTGTCAGCTTATCTAAAGGTACGATATAAACTTTGTCGTACttt                                                                   >  1:291243/1‑140 (MQ=255)
                                                                           tGTCTTCACTATTCTATAAGTCGACGATTTCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAGTTCTAATAAT‑TCTTGTTTTGTCAGCTTATCTAAAGGTACGATATAAACTTTGTCGTACTTAt                                                                  <  1:312806/141‑1 (MQ=255)
                                                                             tCTTCACCATTCAATAAGTCGACGATTTCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAGTTCTAATAAT‑TCTTGTTTTGTCAGCTTATCTAAAGGTACGATATAAACTTTGTCGTACTTAttt                                                                <  1:628359/141‑1 (MQ=255)
                                                                             tCTTCACCATTCAATAAGTCGACGATTTCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAGTTCTAATAAT‑TCTTGTTTTGTCAGCTTATCTAAAGGTACGATATAAACTTTGTCGTACTTAttt                                                                >  2:675507/1‑141 (MQ=255)
                                                                                tCACCATTCAATCAGTCGACGATTTCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAGTTCTAATAAT‑TCTTGTTTTGTCAGCTCATCTAAAGGTACGATATAAACTTTGTCGTACTTATTtttg                                                             <  1:631062/141‑1 (MQ=255)
                                                                                    cATTCAATAAGTCGACGATTTCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAGTTCTAATAAT‑TCTTGTTTTGTCAGCTTATCTAAAGGTACGATATAAACTTTGTCGTACTTATTttagattt                                                         >  2:869839/1‑141 (MQ=255)
                                                                                      ttCAATAAGTCGACGATTTCACTTTTACCCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAGTTCTAATAAT‑TCTTGTTTTGTCAGCTTATCTAAAGGTACGATATAAACTTTGTCGTACTTATTtttgatttga                                                       <  1:483109/141‑1 (MQ=255)
                                                                                      ttCAATAAGTCGACGATTTCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAGTTCTAATAAT‑TCTTGTTTTGTCAGCTTATCTAAAGGTACGATATAAACTTTGTCGTACTTATTtttgatttga                                                       <  1:1132038/141‑1 (MQ=255)
                                                                                        cAATAAGTCGACGATTTTCCTTTAAAGTAAGGAACTAGACGC‑TTTTTTTTGTAATGATTTGCATAGTTCTAATAAT‑TCTTGTTTTGTCAGCTTATCTAAAGGTACGATATAAACTTTGGCGTACTTATTTTtgttttgatt                                                     >  2:1156909/1‑141 (MQ=255)
                                                                                          aTAAGTCGACGATTTCACTTTTAACCATTGAACTTGACGC‑TTTTTTTTGTAATGATTCGCATAGTTCTAATAAT‑TCTTGTTTTGTCAGCTTATCTAAAGGTACGATATAAACTTTGTCGTACTTATTTTTgatttgatttt                                                   >  1:759143/1‑140 (MQ=255)
                                                                                          aTAAGTCGACGATTTCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAGTTCTAATAAT‑TCTTGTTTTGTCAGCTTATCTAAAGGTACGATATAATCTTTGTCGTACTTATTTTTgatttgatttt                                                   >  1:70497/1‑140 (MQ=255)
                                                                                            aaGTCGACGATTTCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTTTTTTTCTTCCCCCCGTTCTCACAAT‑TCCTGTTTTGTCATCTTATCTAAAGTTACGCTATAAACTTTTCCGTACTTCTTTTTGATTTTATTTGTc                                                 >  2:955243/1‑141 (MQ=255)
                                                                                            aaGGCGACGAATTCACTTGTAACCATGGAACTAGTCGC‑TTTTTTTTGTAATGATTCGCATAGTTCTAATAAT‑TCTTGTTTTGTCAGCTTATCGAAAGGTACGATATAAACTTTGTCGTACTTATTTTTGATTTGATTTGTc                                                 <  1:1035425/141‑1 (MQ=255)
                                                                                              gTCGACGATTTCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTTTTTTTTTTCGCATAGTACAAAGAAT‑TCTAGTTTTGTCAGCTTGTCTAACGGTACGATATATACTTTGTCGTACTCATTTTAGATTTGATTTATcac                                               >  2:568357/1‑140 (MQ=255)
                                                                                              gTCGACGATTTCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTTTTATTTTTCGCATAGTTCTAATAAT‑TCTTGTTTTGTCAGCTTATCTAAAGGTACGATGTAACCTTTGTCGTACTTATTTTTGATTGGATTCGTcaa                                               >  2:334904/1‑141 (MQ=255)
                                                                                                  acgaTTTCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGAATAGGTCTAATAAT‑TCTTGTTTTGTCAGCTTATCTAAAGGTACGATATAAACTTTGTCGTACTTATTTTTGATTTGATTTGTcaacagt                                           >  2:301474/1‑139 (MQ=255)
                                                                                                  acgaTTTCACTTTTAACCAGTGAACTAGACGC‑CTTTTTTTGTAATGATTCGCATAGTTCTAATAAT‑TCTTGTTTTGTCAGCCTATCTAAAGGTACGATATAAACTTTGTCGTACTTATTTTTGATTTGATTTGTCAACAAt                                           <  2:398041/141‑1 (MQ=255)
                                                                                                  acaaTTTCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAGTTCTAATAAT‑TCTTGTTTTGTCAGCTTATCTAAAGGTACGATATAAACTTTGTCGTACTTATTTTTGATTTGATTTGTCAACAAt                                           <  1:975792/138‑1 (MQ=255)
                                                                                                    taTTTCACTATTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCTTAGTTCTAATAAT‑TCTTGTTTTGTCAGCTTATCTAAAGGTACGATATAAACTTTGTCGTACTTATTTTTGATTTGATTTGTCAACAATTc                                         <  1:388998/140‑1 (MQ=255)
                                                                                                     aTTTCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAGTTCTAATAAT‑TCTTGTTTTGTCAGCTTATCTAAAGGTACGATATAAACTTTGTCGTACTTATTTTTGATTTGATTTGTCAACAATTCa                                        >  1:510723/1‑141 (MQ=255)
                                                                                                      tttCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTAGCATCGTTCTAATTAT‑TCTTGTTTTTTTAGCTTATCTAAAGGTACGATATAAACTTTGTCGTCCTTATTTTTGATTTGCTTTTTCCACAATTaaa                                       >  2:727261/1‑141 (MQ=255)
                                                                                                       ttCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAGTTCTAATAAT‑TCTTGTTTTGTCAGCTTATCTAAAGGTACGCTATAAAATTTGTCGTACTTATTTTTGATTTTATTTGTCAACAATTCata                                      >  1:846186/1‑139 (MQ=255)
                                                                                                       ttCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAGTACTAATAAT‑TCTTGTTTTGTCAGCTTATCTAAAGGTACGATATAAACTTTGTCGTACTTATTTTTGATTTGATTTGTTCACAATTcgaa                                      >  2:1014610/1‑138 (MQ=255)
                                                                                                        tCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAGTTCTAATAAT‑TCTTGTTTTGTCAGCTTATCTAAAGGTACGATATAAACTTTGTCGTACTTATTTTTGATTTGATTTGTCAACAATTCAaca                                     <  1:826786/141‑1 (MQ=255)
                                                                                                        tCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAGTTCTAATAAT‑TCTTGTTTTGTCAGCTTATCTAAAGGTACGATATAAACTTTGTCGTACTTATTTTTGATTTGATTTGTCAACAATTCAaca                                     >  2:489246/1‑141 (MQ=255)
                                                                                                         cACTTTTAACCAGTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAGTTCTAATAAT‑TCTTGTTTTGTCAGCTTATCTAAAGGTACGATATAAACTTTGTCGTACTTATTTTTGATTTGATTTGTCAACAATTCAacac                                    <  2:688030/141‑1 (MQ=255)
                                                                                                          ccTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAGTTCTAATAAT‑TCTTGTTTTGTCAGCTTATCTAAAGGTACGATATAAACTTTGTCGTACTTATTTTTGATTTGATTTGTCAACAATTCAACACg                                   <  2:1107969/140‑1 (MQ=255)
                                                                                                          ccTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAGTTCTAATAAT‑TCTTGTTTTGTCAGCTTATCTAAAGGTACGATATAAACTTTGTCGTACTTATTTTTGATTTGATTTGTCAACAATCCAACACg                                   <  2:1108037/140‑1 (MQ=255)
                                                                                                          aCTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAGTTCTAATAAT‑TCTTGTTTTGTCAGCTTATCTAAAGGTACGATATAAACTTTGTCGTACTTATTTTTGATTTGATTAGTAAACAATTCAacaca                                   >  2:737816/1‑140 (MQ=255)
                                                                                                           cTTTTAACCATTTAACTAGACGC‑TTTTTTTTGTGATGATTCGCATAGTTCTAATAAT‑TCTTGTTTTGTCAGCTTATCTAAAGGTACGATATAAACTTTGTCGTACTTATTTTTGATTTGATTTGTCAACAATTCAATACGa                                  <  1:815130/141‑1 (MQ=255)
                                                                                                           cTTTTAACCATTGCACTAGACGC‑TTTTTTTTGTTATGATTCGCATAGTTCTAATAAT‑TCTTGTTTTGTCAGCTTATCTAAAGGTACGATATAAACTTTGTCGTACTTATTTTTGATTTGATTTGTCAACAATTCAACACGa                                  <  2:1066941/141‑1 (MQ=255)
                                                                                                           cTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAGTTCTAATAAT‑TCTTGTTTTGTCAGCTTATCTAAAGGTACGATATAAACTTTGTCGTACTTATTTTTGATTTGATTTGTCAACAATTCAACACGa                                  <  2:111638/141‑1 (MQ=255)
                                                                                                             tttAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAGTTCTAATAAT‑TCTTGTTTTGTCAGCTTATCTAAAGGTACGATATAAACTTTGTCGTACTTATTTTTGATTTGATTTGTCAACAATTCAACACGAgg                                <  2:1016872/141‑1 (MQ=255)
                                                                                                               tAACAATTTCACAAGACGT‑TTTTTTTTGTTATGATTCGCATAGTTCTAATAAT‑TCTTGTTTTGGCAGCTTATCTAAAGGTACGATATAAACTTTGTCGTACTTATTTTTGATTTGATTTGTCAACAATTCAACACGAGGAt                              <  2:968645/141‑1 (MQ=255)
                                                                                                                aaCCATTGAACTAGACGC‑TTTTTTTTTTTTTTTTTCTCATAGTTATAAGAAT‑TCTTATTTTGTCATCTTATCTTAAGGTACGATATGTACTTTTTCCTACTTTTTTTTTTTTTGATTTGTCAACAATTCacctcgtttttt                             >  2:607347/1‑130 (MQ=255)
                                                                                                                aaCCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAGTTCTAATAAT‑TCTTGTTTTGTCAGCTTATCTAAAGGTACGATATAAACTTTGTCGTACTTATTTTTGATTTGATTTGTCAACAATTCAACACTAGGAtt                             >  1:110282/1‑141 (MQ=255)
                                                                                                                  ccATTGAACTAGACGC‑TGTTTTTTGTAATGATTCGCATAGTTCTAATAAT‑TCTTGTTTTGTCAGCTTATCTAAAGGTACGATATAAACTTTGTCGTACTTATTTTTGATTTGATTTGTCAACAATTCAACACGAGGATTGt                           <  1:1052610/141‑1 (MQ=255)
                                                                                                                  ccAGTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAGTTCTAATAAT‑TCTTGTTTTGTCAGCTTATCTAAAGGTACGATATAAACTTTGTCGTACTTATTTTTTATTTGTTTTGTCAACAATTCAACACGAGGATTGt                           <  1:758737/141‑1 (MQ=255)
                                                                                                                    aTTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAGTTCTAATAAT‑TCTTGTTTTGTCAGCTTATCTAAAGGTACGATATCAACTTTGTCGTACTTATTTTTGATTTGATTTGTCAACAATTCAACACGAGGATTGTta                         <  1:721269/141‑1 (MQ=255)
                                                                                                                     ttGAACTAGACGC‑TTTTTTTTTTTTTTTTTCTCATCGTTCTAATAAT‑TCTTGTTTTGTCAGCTTATCTAAAGGTACGATATAAGCTTTGTCGTACTTCTTTTTGATTTAATTTGTAAATATTTCTacactctatttgttat                        >  2:984929/1‑129 (MQ=255)
                                                                                                                     ttGAACTAGACGC‑TTTTTTTTGTTTTTTTTCGCATAGTTCTAATAAT‑TCTTGTTTTGTCAGCTTATCTAAAGGTACGATATAAACTTTGTGGTACTTATTTTTGATTTGATTTGTCAACAATTCAACACGTGGATTGTtat                        >  2:1188276/1‑141 (MQ=255)
                                                                                                                     ttGAACTAGACGC‑TTTTTTTTGTCATGATTCGCATAGTTCTAATAAT‑TCTTGTTTTGTCAGCTTATCTAAAGGTACGATATAAACTTTGTCGTACTTATTTTTGATTTGATTTGTCAAGCATTCAAAACGTGGTTTGTgat                        >  1:72178/1‑141 (MQ=255)
                                                                                                                     ttGAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAGTTCTAATAAT‑TCTTGTTTTGTCAGCTTATCTAAAGGTACGATATAAACTTTGTCGTACTTATTTTTGTTTTGATTTTTCAACAATTCAACACGAGGATTGTtat                        >  1:747315/1‑141 (MQ=255)
                                                                                                                        aaCTAGACGC‑TTTTTTTTGTATAGAATCTCATAGTTCTAATAAT‑TCTTGTTTTGTCAGCTTATCTAAAGGTACGATATAAACTTTGTCGTACTTATTTTTGATTTGATTTGTCAACAATTCAACACGAGGATTGTTATAcc                     >  1:286134/1‑141 (MQ=255)
                                                                                                                        aaCTAGACGC‑TTTTTTTTGTAATGATTCGCATAGTTCTAATAAT‑TCTTGTTTTGTCAGCTTATCTAAAGGTACGATATAAACTTTGTCGTACTTTTTTTTGATTTGTTTTGTCAACAATTCAACACGAGGATTGTTATAcc                     >  1:344874/1‑141 (MQ=255)
                                                                                                                        aaCTAGACGC‑TTTTTTTTGTAATGATTCGCATAGTTCTAATAAT‑TCTTGTTTTGTCAGCTTATCTAAAGGTACGATATAAACTTTGTCGTACTTATTTTTGATTTGATTTGTCAACAATTCAACACGAGGATTGTTATAcc                     >  1:942457/1‑141 (MQ=255)
                                                                                                                                gC‑TTTTTTTTGTAATGATTCGCATAGTTCTAATAAT‑TCTTGTTTTGTCAGCTTATCTAAAGGTACGATATAAACTTTGTCGTACTTATTTTTGATTTGATTTGTCAACAATTCAACACGAGGATTGTTATCCCCTTCAGCt             >  1:43457/1‑141 (MQ=255)
                                                                                                                                       ttttGTAATGATTCGCATAGTTCTAATAAT‑TCTTGTTTTGTCAGCTTATCTAAAGGTACGATATAAACTTTGTCGTACTTATTTTTGATTTGGTTTGTCAACAATTCAACACGAGGATTGTTATACCCTTCAGCTGGATAc       >  1:390917/1‑141 (MQ=255)
                                                                                                                                       ttttGTAATGATTCGCATAGTTCTAATAAT‑TCTTGTTTTGTCAGCTTATCTAAAGGTACGATATAAACTTTGTCGTACTTATTTTTGATTTGATTTGTCAACAATTCAACACGAGGATTGTTATATCCTTCAGCTGGATAc       >  1:932773/1‑141 (MQ=255)
                                                                                                                                        tttGTAATGATTCGCATAGATCTAATAAT‑TCTTGTTTTGTCAGCTTATCTAAAGGTACGATATAAACTTTGTCGTACTTATTTTTGATTTGATTTGTCAACAATTCAACACGAGGATTGTTATACACTTCAGCTGGATACa      >  2:1070767/1‑141 (MQ=255)
                                                                                                                                            tAATGATTCGCATAGTTCTAATAAT‑TCTTGTTTTGTCAGCTTATCTAAAGGTACGATATAAACTTTGTCGTACTTATTTTTGATTTGATTTGTCAACAATTCAACACGAGGATTGTTATACCCTTCAGCTGGATACAACTc  >  1:849467/1‑141 (MQ=255)
                                                                                                                                             |                                                                                                                                            
TAAGTACTCATCCTCTGAATTATGGTCAATCTTTTCAAGTGATTTAAATTTGACAAGCAAATCATCAATCGTCATTGTCTTCACCATTCAATAAGTCGACGATTTCACTTTTAACCATTGAACTAGACGC‑TTTTTTTTGTAATGATTCGCATAGTTCTAATAAT‑TCTTGTTTTGTCAGCTTATCTAAAGGTACGATATAAACTTTGTCGTACTTATTTTTGATTTGATTTGTCAACAATTCAACACGAGGATTGTTATACCCTTCAGCTGGATACAACTC  >  CP000730/2104862‑2105141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑