Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,562,956 A→C 7.2% I211L (ATT→CTT)  USA300HOU_2424 → APC family amino acid‑polyamine‑organocation transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,562,9560AC 7.2% 277.6 / 9.1 110I211L (ATT→CTT) USA300HOU_2424APC family amino acid‑polyamine‑organocation transporter
Reads supporting (aligned to +/- strand):  ref base A (52/48);  new base C (7/2);  total (60/50)
Fisher's exact test for biased strand distribution p-value = 1.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01

Only 100 of 123 total aligned reads displayed.

TTGGTCTTGTATTAATCTTTTTCTCATTTAAAACACATTATGGACATGCATCATTCACAAACTTAATCAGTCACGGTGGCATGTTCCCTGGTGGAACATTTGGTTTCTTAATGTCATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACTTATAGGTGTAACTGCTGGTGAAACGAAAGATCCTGAAAAAACCTTACCGAAAGCAATTAATAATGTACCTATCCGTATTTTATTATTCTATATCGGTGGTCTATTAGTAATTATGTCAGTCATACC  >  CP000730/2562816‑2563095
                                                                                                                                            |                                                                                                                                           
ttGGTCTTGTATTAATCTTTTTCTCATTTAAAACACATTATGGACATGCATCATTCACAAACTTAATCAGTCACGGTGGCATGTTCCCTGGTGGAACATTTGGTTTCTTAATGTCATTCCAAATTGCTGTATAttcattca                                                                                                                                             <  1:872660/141‑1 (MQ=255)
 tGGTCTTGTATTAATCTTTTTCTCATTTAAAACACATTATGGACATGCATCATTCACAAACTTAATCAGTCACGGGGGCATGTTGAGGACACCAACATATGGTTTCTTAATATCATTCCAAATTTCTGTATATtcattcat                                                                                                                                            >  1:225545/1‑141 (MQ=255)
  ggTCTTGTATTAATCTTTTTCTCATTTAAAACACATTATGGACATGCATCATTCACAAACTTAATCAGTCACGGTGGCATGTTCGCTGGTGGTACATTTGGTTTCTTAATGTCATTCCAAATTGCTGTATATTcattcatt                                                                                                                                           >  1:883991/1‑141 (MQ=255)
  ggTCTTGTATTAATCTTTTTCTCATTTAAAACACATTATGGACATGCATCATTCACAAACTTAATCAGTCACGGTGGCATGTTCCCTGGTGGAACATTTGGTTTCTTAATGTCATTCCAAATTGCTGTATATTcattcatt                                                                                                                                           <  1:505625/141‑1 (MQ=255)
       tttATGAATCTTTTTCTCATTGTAAGCACATTATGGACATGTATCATTCACAAACTTAATCAGTCACGGTGGCATGTTCCCTGGTGGAATATTTGGTTTCTTAATGTCATTCCAAATTGCTGTATATTCATTCATTGGTAt                                                                                                                                      <  2:1182664/139‑1 (MQ=255)
        gTATTAATCTTTTTCTCTTTTAAAACACATTATGGACATGCATCATTCACAAACTTAATCAGTCACGGTGGCATGTTCCCTGGTGGAACATTTGGTTTCTTAATGTCATTCCAAATTGCTGTATATTCATTCATTGGTAtt                                                                                                                                     <  1:298590/141‑1 (MQ=255)
            tAATCTTTTTCTCATTTAAAACACATTATGGACATGCATCATTCACAAACTTAATCAGTCACGGTGGCATGTTCCCTGGTGGAACATTTGGTTTCTTAATGTCATTCCAAATTGCTGTATATTCATTCATTGGTATTGAAc                                                                                                                                 <  2:1169858/141‑1 (MQ=255)
             aaTCTTTTTCTCATTTAAAACACATTTTGGACATGCATCATTCACAAACTTAATCAGTCACGGTGGCATGTTCCCTGGTGGAACATTTGGTTTCTTAATGTCATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACt                                                                                                                                <  2:759139/141‑1 (MQ=255)
             aaTCTTTTTCTCATTTAAAACACATTATGGACATGCATCATTCACAAACTTAATCAGTCACGGTGGCATGTTCCCTGGTGGAACATTTGGTTTCTTAATGTCATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACt                                                                                                                                <  1:942615/141‑1 (MQ=255)
                   tgtctcATTTAAAACACGTTATTGACGTGCATCATTCACAAACTTAATCAGTCACGGTGGCATGTTCCCTGGTGTAACATTTGGTTTCTTAGTGTCATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACTTATAgg                                                                                                                          <  2:1030986/139‑1 (MQ=255)
                    ttCTCATTTAAAACACATTATGGACATGCATCATTCACAAACTTAATCAGTCACGGTGGCATGTTCCCTGGTGGAACCTTTGGTTTCTTAATGTCATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACTTATAGgt                                                                                                                         >  1:808265/1‑141 (MQ=255)
                    ttCTCATTTAAAACACATTATGGACATGCATCATTCACAAACTTAATCAGTCACGGTGGCATGTTCCCTGGTGGAACATTTGGTTTCTTAATGTCATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACTTATAGgt                                                                                                                         <  1:9677/141‑1 (MQ=255)
                    ttCTCATTTAAAACACATTATGGACATGCATCATTCACAAACTTAATCAGTCACGGTGGCATGTTCCCTGGTGGAACATTTGGTTTCTTAATGTCATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACTTATAGgt                                                                                                                         >  1:1011417/1‑141 (MQ=255)
                      ctcATTTAAAACACATTATGGACATGCATCATTCACAAACTTAATCAGTCACGGTGGCATGTTCCCTGGTGGAACATTTGGTTTCTTAATGTCATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACTTATCGttgt                                                                                                                       >  1:1046467/1‑141 (MQ=255)
                       tcATTTAAAACACATTATGGACATGCATCATTCACAAACTTAATCAGTCACGGTGGCATGTTCCCTGGTGGAACATTTGGTTTCTTAATGTCATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACTTCTAGGTGTa                                                                                                                      >  2:727355/1‑141 (MQ=255)
                        cATTTAAAACACATTATGGGCATGCATCATTCACAAACTTAATCAGTCACGGTGGCATGTTCCCTGGTGGAACATTTGGTTTCTTAATGTCATTCCAAATTGCTGTATAGTCGTTCATTGGTATTGAACTTATAGGTGTaa                                                                                                                     >  2:838846/1‑141 (MQ=255)
                        cATTTAAAACACATTATGGACATGCATCATTCACAAACTTAATCAGTCACGGTGGCATGTTCCCTGGTGGAACATTTGGTTTCTTAATGTCATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACTTGTAGGTGTaa                                                                                                                     >  2:393428/1‑141 (MQ=255)
                        cATTTAAAACACATTATGGACATGCATCATTCACAAACTTAATCAGTCACGGTGGCATGTTCCCTGGTGGAACATTTGGTTTCTTAATGTCATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACTTATAGGTGTaa                                                                                                                     >  1:799383/1‑141 (MQ=255)
                         aTTTAAAACACATTATGGACATGCATCATTCACAAACTTAATCAGTCACGGTGGCATGTTCCCTGGTGGAACATTTGGTTTCTTAATGTCATTCCAAATTGCTGTATATTCATTCCTTGGTATTGAACTTATAGGTGTacc                                                                                                                    >  1:385935/1‑139 (MQ=255)
                          tttAAAACACATTATGGACATGCATCATTCACAAACTTAATCAGTCACGGTGGCATGTTCCCTGGTGGAACATTTGGTTTCTTAATGTCATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACTTATAGGTGTAAct                                                                                                                   >  2:373628/1‑141 (MQ=255)
                           ttAAAACACATTATGGACATGCATCATTCACAAACTTAATCAGTCACGGTGGCATGTTCCCTGGTGGAACATTTGGTTTCTTAATGTCATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACTTATAGGTGTAActg                                                                                                                  >  2:942662/1‑141 (MQ=255)
                               aaCACATTATGGACATGCATCATTCACAAACTTAATCAGTCACGGTGGCATGTTCCCTGGTGGAACATTTGGTTTCTTAATGTCATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACTTATAGGTGTAACTGCTgg                                                                                                              <  2:1178515/141‑1 (MQ=255)
                               aaCACATTATGGACATGCATCATTCACAAACTTAATCAGTCACGGTGGCATGTCCCTTGGTGGAACATTTGGTTTCTTAATGTCATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACTTATAGGTGTAACTGCTgg                                                                                                              <  1:868338/141‑1 (MQ=255)
                                acacATTATGGACATGCATCATTCACAAACTTAATCAGTCACGGTGGCATGTTCCCTGGTGGAACATTTGGTTTCTTAATGTCATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACGTATAGGTGTAACTGCTGGt                                                                                                             <  1:717865/141‑1 (MQ=255)
                                acacATTATGGACATGCATCATTCACAAACTTAATCAGTAACGGTGGCATGTTCCCTGATGGAACATTTGGTCCTTTAACGTCATCTAATATTGCCGATTAGCAATTTCAGGGCACTGAACCTATAGATGTAACTGCTggg                                                                                                             >  2:103518/1‑140 (MQ=255)
                                acacATTATGGACAGGCTTCATTCACAAACATAATCAGTTATGGGGGCATGTTCCCTGGTGGAACATTTGGTTTCTTAATGTCATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACTTATAGGTGTAACTGCTGGt                                                                                                             <  2:956800/141‑1 (MQ=255)
                                      tATGGACATGCATCATTCACAAACTTAATCAGTCACGGTGGCATGTTCCCTGGTGGAACATTTGGTTTCTTAATGTCATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACTTATAGGTGTAACCGCTGGTGAAACg                                                                                                       >  2:57079/1‑141 (MQ=255)
                                       aTGGACATGCATCATTCACAAACTTAATCAGTCACGGTGGCATGTTCCCTGGTGGAACATTTGGTTTCTTAATGTCATTCCAAATTGCTGTATATTCATTCCTTGGTATTGAACTTATAGGTGTAACTGCTGGTGAAACGa                                                                                                      >  1:629212/1‑141 (MQ=255)
                                         ggACATGCATCATTCACAAACTTAATCAGTCACGGTGGCATGTTCCCTGGTGGAACATTTGGTTTCTTAATGTCATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACTTATAGGGGTAACTGCTGGGGAAACGaag                                                                                                    >  1:144461/1‑140 (MQ=255)
                                          gataTGCATCATTCACAAACTTAATCAGGCACGGTGGCATGTTCCCTGGTGGATCATTTGGTTTCTTAATGTCATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACTTATAGGTGTAACTGCTGGTGAAACGAAAg                                                                                                   <  2:199728/138‑1 (MQ=255)
                                          gACATGCTTCATTCATAAACTTAATCAGTTACGGTGGCATGTTTCCTGGTGGAACATTTGGTTTCTTAATGTCATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACTTATAGGTGTAACTGCTGGTGAAACGAAAg                                                                                                   <  2:689124/141‑1 (MQ=255)
                                          gACATGCATCATTCACAAACTTAATCAGTCACGGTGGCATGTTCCCTGGTGGAACATTTGGTTTCTTAATGTCATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACTTATAGGTGTAACTGCTGGTGAAAAGCAAg                                                                                                   >  1:205030/1‑141 (MQ=255)
                                            cATGCATCATTCACAAACTTAATCAGTCAAGGTGGCATATTGCCTGGTGGAACATTGTGTGTCTTAATGTCATTCCAGATTGCTGTATATTCATTCATTGTTTTTCACCTTGTAGGTGTAATTGCTGGTGAAACGAAAGAt                                                                                                 >  1:226893/1‑141 (MQ=255)
                                              tGCATCATTCACAAACTTAATCAGTCACGGTGGCATGTTCCCTGGTGGAACATTTGGTTTCTTAATGTCATTCCAAATTGCTGTATCTTCATTCATTGGTATTGAACTTATAGGTGTAACTGCTGGTGAAACGAAAGATcc                                                                                               <  1:799379/141‑1 (MQ=255)
                                               gCATCATTCACAAACTTAATCAGTCACGGTGGCATGTTCCCTGGTGGAACATTTGGTTTCTTAATGTCATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACTTATAGGTGTAACTGCTGGTGAAACGAAAGATCCt                                                                                              >  1:5111/1‑141 (MQ=255)
                                                catcatTCACAAACTTAATCAGTCACGGTGGCATGTTCCCTGGTGGAACATTTGGTTTCTTAATGTCATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACTTATAGGTGTAACTGCTGGTGAAACGAAAGATCCTg                                                                                             <  1:965547/141‑1 (MQ=255)
                                                      tCACAAACTTAATCAGTCACGGTGGCATGTTCCCTGGTGGAACATTTGGTTTCTTAATGTCATTCCAAATTGCTGTATATTCATTCCTTGGTATTGAACTTATAGGTGTAACTGCTGGTGAAACGAAAGATCCTGaaaaaa                                                                                       >  2:1022547/1‑141 (MQ=255)
                                                        ataaaCTTAATCAGTTGCGGTTGCATGTTCCCTGGTGGAACATTTGGTTTCTTAATGTAATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACTTATAGGTGTATGTGCTGGTGAAACGAAAGAAACTGAAAAAAcc                                                                                     <  2:789262/139‑1 (MQ=255)
                                                        acaAATTTAATCAGTCACGGTGGCATGTTCCCTGGTGGAACATTTGGTTTCTTAATGTCATTCCCAATTGCTGTATATTCATTCCTTGGTATTGAACTTCTAGGTGTCACTGCTGGTGAAACAGAAGATCCTGAAAAAAcc                                                                                     >  2:928481/1‑141 (MQ=255)
                                                        aaaactttAATCAGTCAGGGTGGAATTTCCCCTGGTGGAACATTTGTTTTTTGATTGTCATTCCAAATTGCTTTTTATCCTTTAATTGGTATTGAATTTAATGGGGTACTGCTGGTGAAACGAAAGATCCTGAAAAAACCt                                                                                    <  2:1046467/135‑1 (MQ=255)
                                                           aatttAATCAGTCACGGTGGCAGGTTCCCTGGGTGAACATTTGGTTTCTTAATGTCATACCAAATTGCTGTATATTCATTCATTGGTATTGAACTTATAGGAGTAACTGCTGGTGAAACAAAAGATCCTGAAAAAACCTTa                                                                                  <  2:90951/138‑1 (MQ=255)
                                                           aaCTTAATCAGTCACGGTGGCATGTTCCCTGGTGGAACATTTGGTTTCTTAATGTCATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACTTATAGGTGTAACTGCTGGTGAAACGAAAGATCCTGAAAAAACCTTa                                                                                  <  2:220240/141‑1 (MQ=255)
                                                                aaTCAGTCACGGTGGCATGTTCCCTGGTGGAACATTTGGTTTCTTAATGTCATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACTTATAGGTGTAACTGCTGGTGAAACGAAAGATCCTGAAACAACCTTACCGaa                                                                             <  1:845569/141‑1 (MQ=255)
                                                                aaTCAGTCACGGTGGCATGTTCCCTGGTGGAACATTTGGTTTCTTAATGTCATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACTTATAGGTGTAACTGCTGGTGAAACGAAAGATCCTGAAAAAACCTTACCGaa                                                                             <  1:86119/141‑1 (MQ=255)
                                                                 aTCAGTCACGGTGGCATGTTCCCTGGTGGAACATTTGGTTTCTTAATGTCATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACTTATAGGTGTAACTGCTGGTGAAACGAAAGATCCTGAAAAAACCTTACCGaaa                                                                            >  1:3004/1‑141 (MQ=255)
                                                                  tCAGTCACGGTGGCATGTTCCCTGGTGGAACATTTGGTTTCTTAATGTCATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACTTGTAGGTGTTGCGGCTGGTGAAACGAAAGATCCTGAAAAAACCTTACCGAAAg                                                                           >  1:986061/1‑141 (MQ=255)
                                                                   cAGTCACGGTGGCATGTTCCCTGGTGGAACATTTGGTTTCTTGATGTCATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACTTATAGGTGTAACTGCTGTTGAAACGAAAGATCCTGAAAAAACCTTACCGTAAGc                                                                          >  2:265303/1‑141 (MQ=255)
                                                                     gTCACGGTGGCATGTTCCCTGGTGGAACATTTGGTTTCTTAATGTCATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACTTATAGGTGTAACTGCTGGTGAAACGAAAGATCCTGAAAAAACCTTACCGAAAGCaa                                                                        >  2:924309/1‑141 (MQ=255)
                                                                       cACGGTGGCATGTTCCCTGGTGGAACATTTGGTTTCTTAATGTCATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACTTATAGGTGTAACTGCTGGTGAAACGAAAGATCCTGAAAAAACCTTACCGAAAGCAAtt                                                                      >  1:766196/1‑141 (MQ=255)
                                                                       cACGGTGGCATGTTCCCTGGTGGAACATTTGGTTTCTTAATGTCATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACTTATAGGTGTAACTGCTGGTGAAACGAAAGATCCTGAAAAAACCTTACCGAAAGCAAtt                                                                      >  1:139544/1‑141 (MQ=255)
                                                                        aCGGTCGCATGTCCCCTGGTGGAACTTTTGGTTTCTTAATGTTCTTCCAAAGTGCTCTATATTCATTCATTTGTATTGAACTTATAGGGGTAACTGCTGGTGAAACGAAAGATCCTGAAAAAACCTTACCGAAAGCAATta                                                                     <  2:144977/141‑1 (MQ=255)
                                                                        aCGGGGGCTTGTGCCCTGGTGGAACATTTGGTTTCTTAATGTCATTCCAAATTGCTGTATATTCATTCATTGGGATTGAACTTATAGGTGTAACTGCTGGTGAAACGAAAGATCCTGAAAAAACCTTACCGAAAGCAATta                                                                     <  2:626117/141‑1 (MQ=255)
                                                                         cGGTGGCATGTTCCCTGGTGGAACATTTGGTTTCTTAATGTCATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACTTATAGGTGTAACTGCTGGTGAAACGAAAGATCCTGAAAAAACCTTACCGAAAGCAATtaa                                                                    >  1:801314/1‑141 (MQ=255)
                                                                           gTGGCATGTTCCCTGGTGGAACATTTGGTTTCTTAATGTCATTCCCAATTACTGTATATTAATTCATTGGTATTGAACTTATAGGTGTAACTGCTGGTGAAACGAAAGATCCTGAAAAAACCTTACCGAAAGCAATtaata                                                                  >  1:230436/1‑141 (MQ=255)
                                                                            tGGCATGTTCCCTGGTGGAACATTTGGTTTCTTAATGTGATTCCAAATTGCTGTGTATTCATTCATTGGTATTGATCTTATAGGTGTAACTGCTGGTGAAGCGAAAGATCTTGAAAGAACATTACTGAATGCGATtaataa                                                                 >  2:339147/1‑141 (MQ=255)
                                                                            tGGCATGTTCCCTGGTGGAACATTTGGTTTCTTAATGTCATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACTTATAGGTGTAACTGCTGGTGAAACGAAAGATCCTGAAAAAACCTTACCGAAAGCAATtaataa                                                                 >  1:786212/1‑141 (MQ=255)
                                                                               cATGTTCCCTGGTGGAACATTTGGTTTCTTAATGTCATTCCAAATTGGTGTATATTCATTCATTGGTATTGAACTTATAGGTGTAACTGCTGGTGAAACGAAAGATCCTGAAAAAAGCTTACCGAAAGCAATTAATACTGt                                                              >  2:748343/1‑141 (MQ=255)
                                                                               cATGTTCCCTGGTGGAACATTTGGTTTCTTAATGTCATTCCAAATTGCTGTATATTCATTCGTTGGTATTGAACTTATAGGTGTAACTGCTGGTGAAACGAAAGATCCTGAAAAAGCCTTACCGAAAGCAATTAATGATGt                                                              >  2:949796/1‑141 (MQ=255)
                                                                                 tGTTCCCTGGTGGAACATTTGGTTTCTTAATGTCATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACTTATAGGTGTAACTGCTGGTGAAACGAAAGATCCTGAAAAAACCTTACCGAAAGCAATTAATAATGTAc                                                            <  1:175811/141‑1 (MQ=255)
                                                                                 tGTTCCCTGGTGGAACATTTGGTTTCTTAATGTCATTACAAATTGCTGTATATTGATTCATTGGTATTGAACTTATAGGTGTAACTGCTGGTGAAGCGGAAGATCTGGAAAACCACTTATCGACAGAATTGAATAAAGttc                                                            >  2:1052745/1‑139 (MQ=255)
                                                                                 tGTTCCCTGGTGGAACATTTGGTTTCTCAATGTCATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACTTATAGGTGTAACTGCTGGTGAAACGAAAGATCCTGAAAAAACCTTACCGAAAGCAATTAATAATGTAc                                                            <  1:350708/141‑1 (MQ=255)
                                                                                    tCCCTGGTGGAACATTTGGTTTCTTAATGTCATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACTTATAGGTGTAACTGCTGGTGAAACGAAAGATCCTGAAAAAACCTTACCGAAAGCAATTAATAATgcaactc                                                         >  2:292836/1‑135 (MQ=255)
                                                                                       cTGGTGGAACATTTGGTTTCTTAATGTCATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACTTATAGGTGTAACTGCTGGTGAAACGAAAGTTCCTGAAAAAACCTTACCGAAAGCAATTAATAATGTACCTATcc                                                      <  2:165358/141‑1 (MQ=255)
                                                                                           tggAACATTTGGTTTCTTAATGTCATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACTTATAGGTGTAACTGCTGGTGAAACGAAAGATCCTGAAAAAACCTTACCGAAAGCAATTAATAATGTACCTATCCGTAt                                                  <  1:499474/141‑1 (MQ=255)
                                                                                            ggAACATTTGGTTTCTTAATGTCATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACTTATAGGTGTAACTGCTGGTGAAACGAAAGATCCTGAAAAAACCTTACCGAAAGCAATTAATAATGTACCTATCCGTAtt                                                 >  2:1078670/1‑141 (MQ=255)
                                                                                            ggAACATTTGGTTTCTTAATGTAATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACTTAGAGGGGTAACTGCTGGTGAAACGAAAGAGCCTGAAAAAACCTTACCGAAAGCAATTAATAATGTACCTATCCGTAtt                                                 <  2:193310/141‑1 (MQ=255)
                                                                                             gAACATTTGGTTTCTTAATGTCATTACAAATGGCTGTATATTCATTCATTGGTATTGAACTTATAGGTGTAACTGCTGGTGAAACGAAAGATCCTGAAAAAACCTTACCGAAAGCAATTAATAATGTACCTATCCGTAttt                                                <  2:276262/141‑1 (MQ=255)
                                                                                               aCATTTGGTTTCTTAATGTCATTCCAAATTGCTGTATATTAATTCCTTGGTATTGAACTTATGGGCGTAACTGCTGGTGAAACGAAAGATCCTGCAAAAACCTTACCGAAAGCAATTCATAATGTACGCATCCGTATTtta                                              >  2:287350/1‑141 (MQ=255)
                                                                                                cATTTGGTTTCTTAATGTCATTCCAAATTGCTGTATATTCATTCATTGGTATTTAACTTATAGGTGTAACTGCTGGTGAAACGAAAGATCCTGAAAAAACCTTACCGAAAGCAATTAATAATGTACCTATCCGTATTttat                                             <  2:956383/141‑1 (MQ=255)
                                                                                                cATTTGGTTTCTTAATGTCATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACTTATAGGTGTAACTGCTGGTGAAACGAAAGATCCTGAAAAAACCTTACCGAAAGCAATTAATAATGTACCTATCCGTATTttat                                             >  2:515774/1‑141 (MQ=255)
                                                                                                    tGGTTTCTTAATGTCATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACTTATAGGTGTAACTGCTGGTGAAACGAAAGATCCTGAAAAAACCTTACCGAAAGCAATTAATAATGTACCTATCCGTATTTTattatt                                         >  1:1030954/1‑141 (MQ=255)
                                                                                                      ttttCTTAATGTCATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACTTATAGGTGTAACTGCTGGTGAAACGAAAGATCCTGAAAAAACCTTACCGAAAGCAATTAATAATGTACCTATCCGTATTTTATTATTCt                                       <  2:852491/140‑1 (MQ=255)
                                                                                                           ttAATGTCATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACTTATAGGTGTCAGGAAGCGTGAAACGAAAGATCCTGAAAAAACCTTACCGAAAGCAATTAATAATGTACCTATCCGTATTTTATTATTCTATATc                                  <  1:275559/141‑1 (MQ=255)
                                                                                                             aaTGTCATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACTTATAGGTGTAACTGCTGGTGAAACGAAAGATCCTGAAAAAACCTTACCGAAAGCAATTAATAATGTACCTATCCGCATTTTATTATTCTATATCgg                                >  2:469812/1‑141 (MQ=255)
                                                                                                              aTGTCATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACTTATAGGTGTAACTGCTGGTGAAACGAAAGATCCTGAAAAAACCTTATCGAAAGCAATTAATAATGTACCTATCCGTATTTTATTATTCTATATCggt                               <  1:794150/141‑1 (MQ=255)
                                                                                                              aTGTCATTCCAAATTGCTGTATATTCACTCATTGGTATTGAACTTATAGGTGTAACTGCTGGTGAAACGAAAGATCCTGAAAAAACCTTACCGAAAGCAATTAATAATGTACCTATCCGTATTTTATTATTCTATATCggt                               <  1:850301/141‑1 (MQ=255)
                                                                                                                gTCATTCCAAGTTGCTGTATATTCATTCATTGGTATTGAACTTATAGGTGTAACTGCTGGTGAAACGAAAGATCCTGAAAAAACCTTACCGAAAGCAATTAATAATGTACCTATGCGTATTTTATTATTCTATATCggttg                             >  1:877781/1‑139 (MQ=255)
                                                                                                                gTCATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACTTATAGGTGTAACTGCTGGTGAAACGAAAGATCCTGAAAAAACCTTACCGAAAGCAATTAATAATGTACCTATCCGTATTTTATTATTCTATATCggtgg                             <  1:373628/141‑1 (MQ=255)
                                                                                                                gTCATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACTTATAGGTGTAACTGCTGGTGAAACGAAAGATCCTGAAAAAACCTTACCGAAAGCAATTAATAATGTACCTATCCGTATTTTATTATTCTATATCggtgg                             >  1:202474/1‑141 (MQ=255)
                                                                                                                gTCATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACTTATAGGTGTAACTGCTGGTGAAACGAAAGATCCTGAAAAAACCTTACCGAAAGCAATTAATAATGTACCTATCCGTATTTTATTATTCTATATCggtgg                             >  1:378473/1‑141 (MQ=255)
                                                                                                                gTCATTCCAAATTGCTATATAGTCATTCATTGGTATTGAACTTATAGGTGTAACTGCTGGTGAAACGAAAGATCCTGAAAAAACCTTACCGAAAGCAATTAATAATGTACCTATCCGTATTTTATTATTCTATATCggtgg                             >  1:611764/1‑141 (MQ=255)
                                                                                                                  cATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACTTATAGGTGTAACTGCTGGTGAAACGAAAGATCCTGAAAAAACCTTACCGAAAGCAATTAATAATGTACCTATCCGTATTTTATTATTCTATATCGTTGGTc                           >  2:1058283/1‑141 (MQ=255)
                                                                                                                    ttCCAAATTGCTGTATATTCATTCATTGGTATTGAACTTATAGGTGTAACTGCTGGTGAAACGAAAGATCCTGAAAAAACCTTACCGAAAGCAATTAATAATGTACCTATCCGTATTTTATTATTCTATATAGGTGGTCTa                         >  1:311964/1‑141 (MQ=255)
                                                                                                                      ccAAATTGCTGTATATTCATTCATTGGTATTGAACTTATAGGTGTAACTGCTGGTGAAACGAAAGATCCTGAAAAAACCTTACCGAAAGCAATTAATAATGTACCTATCCGTATTTTATTATTCTATATCGGTGGTCTAtt                       <  2:799383/141‑1 (MQ=255)
                                                                                                                        aaaTTGCTGTATATTCATTCATTGGTATTGAACTTATAGGTGTAACTGCTGGTGAAACGAAAGATCCTGAAAAAACCTTACCGAAAGCAATTAATAATGTACCTATCCGTATTTTATTATTCTATATCGGTGGTCTATTAg                     <  1:1158963/141‑1 (MQ=255)
                                                                                                                           ttGCTGTATATTCATTCATTGTTATTGAACTTATAGGTGTAACTGCTGGTGAAACGAAAGATCCTGAAAAAACCTTACCGAAAGCAATTAATAATGTACCTCTGCGTATTTTATTATTCTATATCGGTGGTCTATTAGTaa                  >  1:617709/1‑141 (MQ=255)
                                                                                                                            tGCTGTATATTCATTCCTTGGTATTGAACTTATAGGTGTAACTGCTGGTGAAACGAAAGATCCTGAAAAAACCTTACCGAAAGCAATTAATAATGTACCTATCCGTATTTTATTATTCTATATCGGTGGTCTATTAGTAAt                 >  2:1094258/1‑141 (MQ=255)
                                                                                                                             tcTGTATATTCATTCATTGGTATTGAACTTATAGGTGTAACTGCTGGTGAAACGAAAGATCCTGAAAAAACCTTACCGAAAGCAATTAATAACGTACTTATCCGTATTTTATTATTCTATATCGGTGGTCTATTAGTAAtt                <  1:1127560/140‑1 (MQ=255)
                                                                                                                             gCTGTATATTCATTCATTGGTATTGAACTTATAGGTGTAACTGCTGGTGAAACGAAAGATCCTGAAAAAACCTTACCGAAAGCAATTAATAATGTACCTATCCGTATTTTATTATTTCATATCGGTGGTCGATTAGTACtt                >  2:172223/1‑141 (MQ=255)
                                                                                                                             gCTGTATATTCATTCATTGGTATTGAACTTATAGGTGTAACTGCTGGTGAAACGAAAGATCCTGAAAAAACCTTACCGAAAGCAATTAATAATGTACCTATCCGTATTTTATTATTCTATATCGGTGGTCTATTAGTAAtt                <  1:228673/141‑1 (MQ=255)
                                                                                                                             gCTGTATATTCATTCATTGGTATTGAACTTATAGGTGTAACTGCTGGTGAAACGAAAGATACTGAAAAAACCTTACCGAAAGCAATTAATAATGTACCTATCCGTATTTTATTATTCTATATCGGTGGTCCATTGGTAGtt                >  2:498807/1‑141 (MQ=255)
                                                                                                                                  atatTCATTCATTGGTATTGAACTTATAGGTGTAACTGCTGGTGAAACTAAAGATCCTGAAAAAACCTTACCGAAAGCAATTAATAATGTACCTATCCGTATTTTATTATTCTATATCGGTGGTCTATTAGTAATTATgtc           >  1:524920/1‑141 (MQ=255)
                                                                                                                                   tatTCATTCATTGGCATTGAACTTATAGGTGTAACTGCTGGTGAAACGAAAGATCCTGAAAAAACCTTACCGAAAGCAATTAATAATGTACCTATCCGTATTTTATTATTCTATATCGGTGGTCTATTAGTAATTATgtca          >  1:639706/1‑141 (MQ=255)
                                                                                                                                       ccttcattGAAACTGAACTTACAAATGTAACTGCCGAAAAAACGAAAGATCCTGAAAAAACCTTACCGAAAGCAATTAATAATGTACCTAACCGTATTTTATTATTCTATACCCGTCGCCAATTAGTAATTATgtcagtca      <  2:968170/139‑1 (MQ=255)
                                                                                                                                       cattcattGGTATTGAACTTATAGGTGTAACTGCTGGTGAAACGAAAGATCCTGAAAAAACCTTACCGAAAGCAATTAATAATGTACCTATCCGTATTTTATTATTCTATATCGGTGGTCTATTAGTAATTATgtcagtca      >  1:663568/1‑141 (MQ=255)
                                                                                                                                         ttcattGGTATTGATCTTATAGGTGTAACTGCTGGTGAAACGAAAGATCCTGAAAAAACCTTACCGAAAGCAATTAATAATGTACCTATCCGTATTTTATTATTCTATATCGGTGGTCTATTAGTAATTATGTCAGTCATa    <  2:1011417/141‑1 (MQ=255)
                                                                                                                                         ttcattGGTATTGAACTTATAGGTGTAACTGCTGGTGAAACGAAAGATCCTGAAAAATCCTTACCGAAAGCAATTAATAATGTACCTATCCGTATTTTATTATTCTATATCGGTGGTCTATTAGTAATTATGTCAGTCATa    <  2:808265/141‑1 (MQ=255)
                                                                                                                                           cattGGTATTGAACTTATAGGTGTAACTGCTGGTGAAACGAAAGATCCTGAAAAAACCTTACCGAAAGCAATTAATAATGTACCTATCCGTATTTTATTATTCTATATCGGTGGTCTATTAGTAATTATGTCAGTCATAcc  <  1:858017/141‑1 (MQ=255)
                                                                                                                                           cattGGTATTGAACTTATAGGTGTAACTGCTGGTGAAACGAAAGATCCTGAAAAAACCTTACCGAAAGCAATTAATAATGTACCTATCCGTATTTTATTATTCTATATCGGTGGTCTATTAGTAATTATGTCAGTCATAcc  <  1:467688/141‑1 (MQ=255)
                                                                                                                                           cattGATATCAAACTTATAGGTCTAACCGCTGGCGAAACGAAAGATCCTGAAAAAACCTTACCGAAAGCAATTAATAATGTACCTATCCGTATTTTATTATTCTATATCGGTAGCCTAGTAGTAATTATGTCAGTCATAcc  <  2:849208/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                           
TTGGTCTTGTATTAATCTTTTTCTCATTTAAAACACATTATGGACATGCATCATTCACAAACTTAATCAGTCACGGTGGCATGTTCCCTGGTGGAACATTTGGTTTCTTAATGTCATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACTTATAGGTGTAACTGCTGGTGAAACGAAAGATCCTGAAAAAACCTTACCGAAAGCAATTAATAATGTACCTATCCGTATTTTATTATTCTATATCGGTGGTCTATTAGTAATTATGTCAGTCATACC  >  CP000730/2562816‑2563095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: