BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	2,774,846	A→G	13.2%	S780S (AGT→AGC)	clfB ←	clumping factor B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,774,846	0	A	G	13.2%	328.8 / 11.2	183	S780S (AGT→AGC)	clfB	clumping factor B
Reads supporting (aligned to +/- strand): ref base A (74/75); new base G (25/3); total (100/83)
Fisher's exact test for biased strand distribution p-value = 6.07e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 184 total aligned reads displayed.

TTGAATCGGAGTCACTATCTGAGTCTGAGTCGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAAT  >  CP000730/2774707‑2774982

ttGAATCGGAGTCACTATCTGAGTCTGAGTCGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTAGCTATCCGAGTCTGAGTCGCTGTCTGGATCTGAATCAc                                                                                                                                         >  1:159081/1‑141 (MQ=255)

 tGAATCGGAGTCACTATCTGAGTCTGAGTCGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGGGTCTGAGTCGCTCTCTGAATCTGAATCGCt                                                                                                                                        >  2:1048401/1‑141 (MQ=255)

  gAATCGGAGTCACTATCTGAGTCTGAGTCGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTg                                                                                                                                       >  2:212634/1‑141 (MQ=255)

   aagcGGAGTCACTATCTGAGTCTGAGTCGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGt                                                                                                                                      <  1:351893/138‑1 (MQ=255)

      gggAGGCACTATCGGGGTCTGACTCGCTATCTGAATCCGAGTCGCTATCCGGGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTg                                                                                                                                   <  2:560116/140‑1 (MQ=17)

        gaattgcgaTCTGAGTCTGAGTCGCTTTCTTAATCGATATCGCTATCTGCGTCTGCGTCGCTATCTCAGTCTGACTTGCCGTCCGCGTCGGTGTCGCGATCTGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGag                                                                                                                                 <  2:903019/133‑2 (MQ=0)

         aGTCACTGTCTGAGTGCGAGTCGCTAGCTGCAGCCGAGTCGCTATGCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAAt                                                                                                                                <  2:756656/141‑1 (MQ=18)

         aGTCACTATCTGAGTCTGAGTCGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCCGAATCTGAATCACTGTCTGCAt                                                                                                                                >  1:175848/1‑141 (MQ=255)

          gTCATTTTCTGAGTCGGAGTCGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATc                                                                                                                               <  2:84194/141‑1 (MQ=255)

          gTCACTATCTGAGTCTGGGTCGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATc                                                                                                                               <  2:8324/141‑1 (MQ=255)

           tCACTATCTGAGTCTGAGTCGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCGGAGTGTGAGTCGTGGTCTGGGTCTGAATCGCTGTCTGAATcg                                                                                                                              >  2:170827/1‑140 (MQ=17)

           tCACTATCTGAGTCTGAGTCGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATATGAAGCCCTGTCTGGATcc                                                                                                                              >  2:530790/1‑141 (MQ=255)

            caatATCTGGGTCTGAGTCGCTATCTGAATCCGAGTCGCTATCCGAGGCTGAGTCGCTATCTGAGGCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATcct                                                                                                                             <  2:207415/138‑2 (MQ=255)

             aCTATCTGAGTCTGAGTCGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGa                                                                                                                            >  1:253270/1‑141 (MQ=255)

              cTATCTGAGTCTGAGTCGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGGATCCGaa                                                                                                                           >  1:743326/1‑141 (MQ=255)

                aTCTGAGTCTGAGTCGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATc                                                                                                                         >  1:142981/1‑141 (MQ=255)

                 tctgagtctgagTCGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGACGCTGAGTCGCTATCCGAGTCTGAGTCGCAGTCTGAGTCTGAATCGCTGTCTGAGTCCGATTCg                                                                                                                        >  2:73947/1‑141 (MQ=17)

                 tctgagtctgagTCGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGATGTCTGATTCTGATTCGCGAACCGAGTCTGAGTCGCTGACTGAATCTGAATTACTGTCTGAATCAGAATct                                                                                                                        >  1:1122238/1‑140 (MQ=17)

                  ctgagtctgagtCGCTATCTGACTCTGCGTCGCTATCCGAGTCTGAGTCGCTGTCTGAGTCTGACTCGCTGTCTGAGTCTGAGTCGCTATCGGGGTGTGAGTGGGTGTCTGAATCTGACTCGCTGTccgcaagtgcgtcgc                                                                                                                       >  2:453093/1‑127 (MQ=11)

                  ctgagtctgaggCGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGc                                                                                                                       <  1:48648/141‑1 (MQ=255)

                          gagtcGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTatctgaa                                                                                                               <  1:212634/141‑1 (MQ=255)

                            gtcGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAGTCGCTatctgaaac                                                                                                             >  2:414069/1‑139 (MQ=255)

                            gtcGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAAGCGCTATCTGAGTCTGAGTCGCTGTCTGGATCTGAATCACAGTCAGGATCTGAGTGGCAatctggatg                                                                                                             >  2:939069/1‑140 (MQ=14)

                             tcGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGACTCTGAGTCGCTGTCTGAGTCGGAATCGCTatctggacct                                                                                                            >  2:614497/1‑136 (MQ=14)

                             tcGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGACTCTGAATCACTGTCTGAATCCGAATCGCTatctgcatct                                                                                                            >  1:288077/1‑141 (MQ=255)

                              cGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTatctgaatctg                                                                                                           >  1:669649/1‑141 (MQ=255)

                              cGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTatctgaatctg                                                                                                           <  2:678615/141‑1 (MQ=255)

                                cTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAGTCGGAATCGCGatctgagtcagca                                                                                                         >  2:1197313/1‑137 (MQ=17)

                                cTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTAtctgaatctgaa                                                                                                         >  1:841874/1‑141 (MQ=255)

                                 tATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATctgaatctgaat                                                                                                        <  1:949960/141‑1 (MQ=255)

                                  aTCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCtgaatctgaatc                                                                                                       >  1:259360/1‑141 (MQ=255)

                                   tCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTGGCTGTCTGAATCCGAATCGCGATACGGATCTGAATCg                                                                                                      >  2:405633/1‑141 (MQ=14)

                                    cTGATTCCGAGTCGCTTTCCGGGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGc                                                                                                     <  2:71664/141‑1 (MQ=255)

                                    cTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTTAGACTTTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGc                                                                                                     <  1:1178012/141‑1 (MQ=255)

                                     tGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCt                                                                                                    >  1:1053018/1‑141 (MQ=255)

                                        aTCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATc                                                                                                 >  1:424696/1‑141 (MQ=255)

                                         tCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAATCTGAATCGCTGTcc                                                                                                >  2:335053/1‑141 (MQ=255)

                                          ccGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCg                                                                                               <  1:186106/141‑1 (MQ=255)

                                          ccGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCg                                                                                               >  1:22306/1‑141 (MQ=255)

                                               tCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCt                                                                                          >  2:396415/1‑141 (MQ=255)

                                                  ttatgcggttCTGAGCTGCTATCTGAGTGTGAAGGGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAg                                                                                       <  2:30222/132‑1 (MQ=18)

                                                     tCCGAGTCTGGGTCGCTATCTGAGGCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCg                                                                                    <  2:907994/141‑1 (MQ=255)

                                                        gAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTg                                                                                 <  2:1119293/141‑1 (MQ=255)

                                                          gTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATGCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGtc                                                                               >  1:792975/1‑141 (MQ=255)

                                                          gTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAATCTGAGTCGCTGTCTGAATCTGCATCGCTGGATGAGTCGGCATCGCTGGCGGCATCTGAATCGCTCGCCGAGCCAGAGTCGCTGtc                                                                               >  2:63801/1‑141 (MQ=14)

                                                          gTCTGAGTCGCTATCTGAGTCTGAATCGCTGGCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGCATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGtc                                                                               <  1:232347/141‑1 (MQ=255)

                                                           tCTGGGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAGTCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGtct                                                                              <  2:1029371/141‑1 (MQ=255)

                                                            gtGATTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGtctg                                                                             <  2:766118/140‑1 (MQ=255)

                                                            cTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGGATCAGAATCGCGATCTGGATCTGAGACGCTATCAGAAGCTGAGACGCGGtctg                                                                             >  2:971676/1‑141 (MQ=14)

                                                            cTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGtctg                                                                             <  1:208619/141‑1 (MQ=255)

                                                              gAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCTGAGTCTGAGTCGCTGtctgaa                                                                           >  1:767953/1‑141 (MQ=255)

                                                              gAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGGGTCGCTGtctgat                                                                           >  1:338903/1‑140 (MQ=255)

                                                               aGTCGCTTTCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGtctgaat                                                                          <  1:883461/141‑1 (MQ=255)

                                                               aGTCGCTATCTGAGTCTGAATCGCTATCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTACCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGtctgaat                                                                          <  1:629442/141‑1 (MQ=255)

                                                                 tCGCTTTCTGGGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAGTCACTGTCTGAGTCCGAGTCGCTATCTGAATCTGATTCGCTATCCGAGTCTGAGTCGCTGtctgaatct                                                                        <  2:1092974/141‑1 (MQ=14)

                                                                 tCGCTATCTGAGTCTGAATCGCTGTCTGACTCGGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAATCCGAATCGCTGTCTGAGTCTGAATCGCTATCCGAGTCTGAGTCGCTGtctgaatct                                                                        >  2:496507/1‑141 (MQ=17)

                                                                  cGCTATCTGAGTCTGAATCGCTGTCTGAGGCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCGGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGtctgaatctg                                                                       <  1:711762/141‑1 (MQ=255)

                                                                      aTCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAGTCTGAATCACTGTCTGAATCCGAATCGCTGTCTGAGTCTGAGTCGCTATCGGAGTCTGAGGCGCTgtctgagtctgactg                                                                   >  2:610134/1‑138 (MQ=14)

                                                                      aTCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGACTCACTGTCTGAATCCGAATCGCTGTCTGAGTCGGAGTCGCTGTCTGAGTCCGCGGCACTGTCtgagtctgaatc                                                                   >  2:279876/1‑141 (MQ=14)

                                                                      aTCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCtgaatctgaatc                                                                   >  2:1193141/1‑141 (MQ=255)

                                                                      aTCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCtgaatctgaatc                                                                   <  1:805778/141‑1 (MQ=255)

                                                                          gAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTg                                                               >  2:526703/1‑141 (MQ=255)

                                                                           aGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGt                                                              <  1:937727/141‑1 (MQ=255)

                                                                              cgtattCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTg                                                           <  2:633966/136‑1 (MQ=11)

                                                                                          ctgattctgagtCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGc                                               <  1:412143/141‑1 (MQ=255)

                                                                                          ctgagtctgagtCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGGGTCGCTGTCTGAACCTGAATCGCTGTCTGAGGCCGGATCGc                                               >  2:629649/1‑141 (MQ=255)

                                                                                          ctgagtctgagtCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTGTGAGTCCGAATCGc                                               >  2:12192/1‑141 (MQ=255)

                                                                                          ctgagtctgagtCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGc                                               <  1:198639/141‑1 (MQ=255)

                                                                                          ctgagtctgagtAGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTATCTGAATCAGAGTCACTGTCTGAGTCTGACTCGCTAACAGAGTCTGAGCCGCTGTCTGAGACTGAGTCGCTGTCTGGGTCTGACTcac                                               >  2:481622/1‑139 (MQ=11)

                                                                                           tgagtctgagtcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCTGAATCGCTATCTGAATCTGAATCGCTATCCGAGGCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCt                                              <  2:763765/141‑1 (MQ=255)

                                                                                               tctgagtcGCTATCCGAGTCTGAGTCGCTATCTGAATCTGAATCACTGTCTGAATCGGAGTCGCTGTCTGAATCTGAATCGCTGGCGGAGGCTGAGTCGCTGTCCGACTCTGAATCGCTCGCGGAGTGCGacgcactgtta                                          >  2:382730/1‑131 (MQ=11)

                                                                                                  gagtcGCCATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTatctgaa                                       <  2:443633/141‑1 (MQ=255)

                                                                                                       ccTATCCGTGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTatctgaatctga                                  <  1:135494/140‑1 (MQ=255)

                                                                                                        cTATCCGAGTCTGAGTCGCTGTCTGAATGGGAGTCAGTGTCTGAGGCAGAATGGCTATCTGATGCTGAATAGCTAGGTGGGTGTGGGACGATTTCTGAAACAGAATCGATGGCTGAGACCGAATAGCTATCTGAATCTGAg                                 >  2:780203/1‑141 (MQ=12)

                                                                                                        cTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCt                                                                                                                                    >  2:804945/1‑42 (MQ=40)

                                                                                                        cTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAg                                 >  1:1094855/1‑141 (MQ=255)

                                                                                                            ccGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGGCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTGCCTGAATCTGAGACGc                             >  2:1005446/1‑141 (MQ=17)

                                                                                                            ccGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGc                             <  2:1061573/141‑1 (MQ=255)

                                                                                                                gTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCAGTATGAGTCCGAATCGCTATCTGAATCTGAGTCGCTgtc                         >  1:921956/1‑141 (MQ=255)

                                                                                                                 tCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTgtct                        <  1:1007254/141‑1 (MQ=255)

                                                                                                                 tCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTgtct                        <  2:745457/141‑1 (MQ=255)

                                                                                                                   tGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCGGAGTCGCTgtctga                      >  2:157563/1‑141 (MQ=255)

                                                                                                                   ggggttgttGTTTGGATCTGGATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTAGCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTgtctga                      <  2:822137/133‑1 (MQ=17)

                                                                                                                     ggTCGCTGTCTGAATCTGAATCACTCTCTGAATCCGAATCGCTATCTGAATCTGACTCGCTATCCGAGTCTGAGTCGCTTTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTgtctgagt                    <  2:876849/140‑1 (MQ=255)

                                                                                                                      gTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCTGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTatctgagac                   >  2:570912/1‑139 (MQ=255)

                                                                                                                      gTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTgtctgggtc                   >  1:756506/1‑141 (MQ=255)

                                                                                                                      gTCGCTGTCGGCATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTgtctgagtc                   <  1:1034827/141‑1 (MQ=255)

                                                                                                                        cGCTTTCTTAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTgtctgagtctg                 <  1:266107/141‑1 (MQ=255)

                                                                                                                        cGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTgtctgagtctg                 <  2:22306/141‑1 (MQ=255)

                                                                                                                         gCTTTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTgtctgagtctga                <  1:170681/141‑1 (MQ=255)

                                                                                                                              ctgaatctgaatCACTGTCTGAATCCGAATCGCTATGTGACTCTGAATCGCTATCTGAGTCTGATTGGCTGTCTGACTCTGAATGGCTGTCTGAGTCTGAATCACTATCTGAATCTGAGTCGCTGTCTGGGTCTGAATCGc           >  2:507711/1‑141 (MQ=14)

                                                                                                                              ctgaatctgaatCACTGTCTGAATCCGAATCGCTATCTGAAACTGAACTGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGc           <  1:620820/141‑1 (MQ=255)

                                                                                                                              ccgaatctgactcACTGTCTGAACCCGAATCGCTATCTGATTCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGc           <  2:1054520/139‑1 (MQ=255)

                                                                                                                                gaatctgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTa         <  1:526703/141‑1 (MQ=255)

                                                                                                                                gaatctgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTa         <  1:396415/141‑1 (MQ=255)

                                                                                                                                  atctgaatcACTGTCGGAATCCGAATCGCTATCTGAACCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatc       <  1:414069/141‑1 (MQ=255)

                                                                                                                                    ttgaagcACTGTCTGAATCCGAAGCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctg     <  1:464789/140‑1 (MQ=255)

                                                                                                                                    ctgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctg     >  1:202800/1‑141 (MQ=255)

                                                                                                                                     tgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctga    >  2:311669/1‑141 (MQ=255)

                                                                                                                                       agtcACTGTCTGAGGCCGAGTCGCTATCTGAATCTGAATCGCTCTCTGAGTAAAAATCGCTCTCTGAATCTGCGTCGCTTTCTGAGTCCGAATCGCTAGCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaat  <  2:1149866/139‑1 (MQ=11)

TTGAATCGGAGTCACTATCTGAGTCTGAGTCGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAAT  >  CP000730/2774707‑2774982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`