Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 745,728 A→G 16.8% K9E (AAA→GAA)  USA300HOU_0690 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730745,7280AG16.8% 55.8 / 6.0 29K9E (AAA→GAA) USA300HOU_0690hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (11/13);  new base G (3/2);  total (14/15)
Fisher's exact test for biased strand distribution p-value = 6.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01

ACAGTTTGAAGAAATCGTAGTTCAATAATGTTAATTGTGAAAATGTAT‑‑‑‑‑‑ATAAACATAAAAAAATCATGTATAATATATGTTGTTAATTAAACAGTTCGAAAGCGAGATGACATTATGGGACGTAAATGGAATAACATTAAAGAAAAAAAGGCCCAAAAAGATAAAAACACAAGTAGAATATATGCGAAATTTGGTAAGGAGATTTATGTTGCAGCAAAATCTGGTGAACCCAATCCAGAATCTAACCAAGCTTTAAGGTTGGTGCTTGA  >  CP000730/745590‑745858
                                                                                                                                                |                                                                                                                                  
aCAGTTTGAAGAAATCGTAGTTCAATAATGTTAATTGTGAAAATGTAT‑‑‑‑‑‑ATAAACATAAAAAAATCATGTATAATATATGTTGTTAATTAACCAGTTCGAAAGCGAGATGACATTATGGGACGTAAATGGAATAACATTaa                                                                                                                                   <  2:431251/140‑1 (MQ=255)
   gTTTGAAGAAATCGTAGTTCAATAATGTTAATTGTGAAAATGTAT‑‑‑‑‑‑ATAAACATAAAAAAATCATGTATAATATATGTTGTTAATTAAACAGTTCGAAAGCGAGATGACATTATGGGACGTAAATGGAATAACATTAAAGa                                                                                                                                <  1:63932/140‑1 (MQ=255)
      tGAAGAAATCGTATTTCAATAATGTTAATTGTGAAAATGTAT‑‑‑‑‑‑CTAAACATAAAAAAATCCTGTATAATATATGTTGTTAATTAAACAGTTCGAAAGCGAGATGACATTATGGGACGTAAATGGAATAACATTAAAGaaac                                                                                                                             >  2:168098/1‑139 (MQ=255)
             aTCTTAGTTAAATAATTTTAATTTTGAAAATGTAT‑‑‑‑‑‑ATAAACATAAAAAAATCATGTATAATATATGTTGTTAATTAAACAGTTCGAAAGCGAGATGACATTATGGGACGTAAATGGAATAACATTAAAGAAAAAAAGGcc                                                                                                                      <  2:422213/140‑1 (MQ=255)
             aTCGTAGTTCAATAATGTTAATTGTGCAAATGTAT‑‑‑‑‑‑ATAAAGATAAAAAAATCATGTATAATATATGTTGTTAATTAAACAGTTCGAAAGCGAGATGACATTATGGGACGTAAGTGGAATCACATTAAAGAAAAAAAggga                                                                                                                      >  2:139961/1‑138 (MQ=255)
             aTCGTAGTTCAATAATGTTAATTGTGAAAATGTAT‑‑‑‑‑‑ATAAACATAAAAAAATCATGTATAATATATGTTGTTAATTAAACAGTTCGAAAGCGAGATGACATTATGGGACGTAAATGGAATAACATTAAAGAAAAAAAGGcc                                                                                                                      <  1:204708/140‑1 (MQ=255)
                    ttCAATCTTGTGAATTGTGAAAACGTAT‑‑‑‑‑‑ATAAACATAAAAAAATCATGTATAATATATGTTGTTAATTAAACAGTTCGAAAGCGAGATGACATTATGGGACGTAAATGGAATAACATTAAAGAAAAAAAGGCCCAAAAAg                                                                                                               <  2:377992/140‑1 (MQ=255)
                           atGTTAATTGTGAAAATGTATATAAACATAAACATAAAAAAATCATGTATAATATATGTTGTTAATTAAACAGTTCGAAAGCGAGATGACATTATGGGACGTAAATGGAATAACATTAAAGAAAAAAAGGCCCAAAAAGa                                                                                                              <  2:473983/140‑1 (MQ=255)
                           atGTTAATTGTGAAAATGTATATAAAAATAAACAAAAAAAAATCATGTATAATATATGTTGTTAATTAAACAGTTCGAAAGCGAGATGACATTATGGGACGTAAATGGAATAACATTAAAGAAAAAAAGGCCCAAAAAGa                                                                                                              <  2:473959/140‑1 (MQ=255)
                               tAATTGTGAAAATGTAT‑‑‑‑‑‑ATAAACATAAAAAAATCATGTATAATATATGTTGTTAATTAAACAGTTCGAAAGCGAGATGACATTATGGGAAGTAAATGGAATAACATTAAAGAAAAAAAGGCCCAAAAAGATaaaaaaaaa                                                                                                    >  1:521735/1‑136 (MQ=255)
                                       aaaaTGTAT‑‑‑‑‑‑ATAAACATAAAAAAATCATGTATAATATATGTTGTTAATTAAACAGTTCGAAAGCGAGATGACATTATGGGACGTAAATGGAATAACATTAAAGAAAAAAAGGCCCAAAAAGATAAAAACACAAGTAGAat                                                                                            >  1:298722/1‑140 (MQ=255)
                                           tGTAT‑‑‑‑‑‑ATAAACATAAAAAAATCATGTATAATATATGTTGTTAATTAAACAGTTCGAAAGCGAGATGACATTATGGGACGTAAATGGAATAACATTGAAGAAAAAAAGGCCCAAAAAGATAAAAACACAAGTAGAatatat                                                                                        >  2:458108/1‑140 (MQ=255)
                                           tGTAT‑‑‑‑‑‑ATAAACATAAAAAAATCATGTATAATATATGTTGTTAATTAAACAGTTCGAAAGCGAGATGACATTATGGGACGTAAATGGAATAACATTGAAAAAAAAAAGGCCCAAAAAGATAAAAACACAAGTAGAatatat                                                                                        >  1:119176/1‑140 (MQ=255)
                                                    aacataaCATAAAAAATTGATGTATAACTTATGTTGTTAATTAAACAGTTCGAAAGCGAGATGACATTATGGGACGTAAACGGAATAACATTAAAGAAAAAAAGGCCCAAAAAGATAAAAACACAAGTAGAATATATGCg                                                                                     <  2:369400/135‑1 (MQ=255)
                                                               aaaaaaTCATGTATAATATATGTTGTTAATTAAACAGTTCGAAAGCGAGATGACATTATGAGACGTAAATGGAATAACATTAAAGAAAAAAAGGCCCAAAAAGATAAAAACACAAGTAGAATATATGAGCAACTTGGTaa                                                                          >  2:1217/1‑140 (MQ=255)
                                                                 aaaaTCATGTATAATATATGTTGCTTCTAAATTAGTTCGAAAGCGAGATGACATTATGGGACGTAAATGGAATAACATTGAAGAAAAAAAGGCCCAAAGAGAGAAAAACACAAGTAGAATATATGCGAAATTTGAGAAgg                                                                        >  2:529353/1‑140 (MQ=255)
                                                                      cATGAATAATATAATTTTATAATAAAACGTTTCAAAAGCGAGATAACATTATGGAACGTAAAAGAAATAACAATAAAAAAAAAAAGGCTCAAAAAGATAAAAACACAAGTAGATAACCGGCGTGAGTTGGTAAGGAGAtt                                                                   <  2:391155/140‑1 (MQ=255)
                                                                        tGTATAATATATGTTGTTAATTAAACAGTTCGAAAGCGAGATGACATTATGGGACGTAAATGGAATAACATTAAAGAAAAAAAGGCCCAAAAAGATAAAAACACAAGTAGAATATATGCGAAATTTGGTAAGGAGATTTa                                                                 >  1:312043/1‑140 (MQ=255)
                                                                                atatGTTGTTAATTAAACAGTTCGAAAGCGAGATGACATTATGGGACGTAAATGGAATAACATTAAAGAAAAAAAGGCCCAAAAAGATAAAAACACAATTAGAATATATGCGAAATTTGGTAAGGAGATTTATGTTgcag                                                         <  1:377213/140‑1 (MQ=255)
                                                                                atagGTTGTTAAGTAAACAGTTCGAAAGCGAGATGACATTGTGGGACGTAAATGGAATAACATTAAAGAAAAAAAGGCCCCAAAAGATTTAAACACAAGTAGAATATATGCGAAATTTGGTAAGGAGATTTATGTTgccg                                                         <  2:144060/140‑3 (MQ=255)
                                                                                              aaaCAGTTCGAAAGCGAGATGACATTATGGGACGTAAATGGAATAACATTAAAGAAAAAAAGGCCCAAAAAGATAAAAACACAAGTAGAATATATGCGAAATTTGGTAAGGAGATTTATGTTGCAGCAAAATCTGGTGaa                                           >  1:115867/1‑140 (MQ=255)
                                                                                              aaaCAGTTCGAAAGCGAGATGACATTATGGGACGTAAATGGAATAACATTAAAGAAAAAAAGGCCCAAAAAGATAAAAACACAAGTAGAATATATGCGAAATTTGGTAAGGAGATTTATGTTGCAGCAAAATCTGGTGaa                                           >  1:346846/1‑140 (MQ=255)
                                                                                                    ttCGAAAGCGAGATGACATTATGGGACGTAAATGGAATAACATTAAAGAAAAAAAGGCCCAAAAAGATAAAAACACAAGTAGAATATATGCGAAATTTGGTAAGGAGATTTATGTTGCAGCAAAATCTGGTGAACCCAAt                                     >  1:528300/1‑140 (MQ=255)
                                                                                                           gCGAGATGACATTATGGGACGTAAATGGAATAACATTAAAGAAAAAAAGGCCCAAAAAGATAAAAACACAAGTAGAATATATGCGAAATTTGGTAAGGAGATTTATGTTGCAGCAAAATCTGGTGAACCCAATCCAGAAt                              <  1:406693/140‑1 (MQ=255)
                                                                                                            cGAGATGACATTATGGGACGTAAATGGAATAACATTAAAGAAAAAAAGGCTCAAAAAGATAAAAACACAAGTAGAATATATGCGAAATTTGGTAAGGAGATTTATGTTGCAGCAAAATCTGGTGAAGCCAATCCAGAATc                             >  2:186080/1‑140 (MQ=255)
                                                                                                                         tGGGACGTAAATGGAATAACATTAAAGGAAAAAAGGCGTAACAAGATCAAAACACAAGTAGAATATATGCGACATTTGGTAAGGAGATGTATGTTGCAGAAAAGTCTGGTGAACACAATACAGCATCTAAcacagcctga                >  2:471922/1‑131 (MQ=255)
                                                                                                                                    tGGAATAACATTAAAGAAAAAAAGGCCCAAAAAGATAAAAACACAAGTAGAATATATGCGAAATTTGGTAAGGAGATTTATGTTGCAGCAAAATCTGGTGAACCCAATCCAGAATCTAACCAAGCTTTAAGGTTGGTGCt     <  2:312043/140‑1 (MQ=255)
                                                                                                                                     gtaacaaCATTAAAGAAAAAAAGGCCCAAAAAGATAACAACACACGTAGGATATATGCGAAATTTGGTAAGGAGATTTATGTTGCAGCAAAATCTGGTGAACCCAATCCAGAATCTAACCAAGCTGTAAGGTTGGTGCtt    <  2:376074/135‑1 (MQ=255)
                                                                                                                                       aaTAACATTGAAGAAAAAAAGGCCCAAAAAGATAAAAACACAAGTAGAATATATGCGAAATTTGGTAAGGAGATTTATGTTGCAGCAAAATCTGGTGAACCCAATCCAGAATCTAACCCAGCTGTAAGGTTGGTGCTTGa  <  2:119176/140‑1 (MQ=255)
                                                                                                                                       aaTAACATTGAAGAAAAAAAGGCCCAAAAAGATAAAAACACAAGTAGAATATATGCGAAATTTGGTAAGGAGATTTATGTTGCAGCAAAATCTGGTGAACCCAATCCAGAATCTAACCAAGCTTTAAGGTTGGTGCTTGa  <  1:458108/140‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                  
ACAGTTTGAAGAAATCGTAGTTCAATAATGTTAATTGTGAAAATGTAT‑‑‑‑‑‑ATAAACATAAAAAAATCATGTATAATATATGTTGTTAATTAAACAGTTCGAAAGCGAGATGACATTATGGGACGTAAATGGAATAACATTAAAGAAAAAAAGGCCCAAAAAGATAAAAACACAAGTAGAATATATGCGAAATTTGGTAAGGAGATTTATGTTGCAGCAAAATCTGGTGAACCCAATCCAGAATCTAACCAAGCTTTAAGGTTGGTGCTTGA  >  CP000730/745590‑745858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: