Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,646,189 G→C 100% A155G (GCA→GGA)  USA300HOU_1531 ← M24B family Xaa‑Pro dipeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,646,1890GC100.0% 107.6 / ‑6.5 35A155G (GCA→GGA) USA300HOU_1531M24B family Xaa‑Pro dipeptidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (18/16);  minor base T (0/1);  total (18/17)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTGCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACG  >  CP000730/1646057‑1646325
                                                                                                                                    |                                                                                                                                        
tcccTTGCAACACCATGGGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTCGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTGTCAGTCATGCCTCCTTTTac                                                                                                                                   <  2:39928/137‑1 (MQ=255)
  aCTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTacaa                                                                                                                                 <  1:70368/140‑1 (MQ=255)
          cgccATGTGGGAAAGCATCTCTATTACCAGATTCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTATCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTaaa                                                                                                                         <  1:477414/138‑1 (MQ=255)
           acCATGTTGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTaaaa                                                                                                                        <  1:448893/140‑1 (MQ=255)
             cATGTTGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAata                                                                                                                      <  1:225068/140‑1 (MQ=255)
               tgtgGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTATAATATTGCCATTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAatata                                                                                                                    >  1:110930/1‑140 (MQ=255)
               tgtgATAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAatata                                                                                                                    >  1:376161/1‑140 (MQ=255)
                          atctctATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGttt                                                                                                         <  2:1879/138‑1 (MQ=255)
                             tctATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAGATATATTCATATGTTtcat                                                                                                      <  1:410043/140‑1 (MQ=255)
                                        aTGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTa                                                                                           <  1:232486/140‑1 (MQ=255)
                                         tGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTATTACAACAGTTAAAATATATTCATATGATACATCAACAATATTAg                                                                                          >  1:219622/1‑140 (MQ=255)
                                          gCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAgc                                                                                         >  1:153793/1‑140 (MQ=255)
                                           cTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATCTTAgct                                                                                        >  2:491089/1‑140 (MQ=255)
                                                       cGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAAtt                                                                            >  2:515499/1‑140 (MQ=255)
                                                          atgatgGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTCTCAGTCATGTCTCCTTTTACAACAGTTAAAATATATTCATATGTTTTATTAACATTATTAGCTGCTTTTTGAATTaaa                                                                         >  2:457660/1‑140 (MQ=255)
                                                               ggTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTTCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAATTATATTCATATGTTTCATCCACAATATTAGATGCTTTTTGAATTAAAGCAAt                                                                    >  2:289195/1‑140 (MQ=255)
                                                                gTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATCTTAGCTGCTTTTTGAATTAAAGCAAtt                                                                   >  2:274467/1‑140 (MQ=255)
                                                                            cTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCATCAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCt                                                       <  2:301209/140‑1 (MQ=255)
                                                                            cTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAACATCt                                                       <  2:69100/140‑1 (MQ=255)
                                                                               aTTCTAGCATTTTGCTTTCTAATATTGCCTTTGATTCTTTTTCAGTCACGCCTCCTTTTACAACCGTTAAAATATATTGATACGTTTCATCAACAATATTTGCTTCTTTTTGAATTAATGCAATTTCGTCCGCATCTTTg                                                    >  2:129621/1‑140 (MQ=255)
                                                                                      cATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTCAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTGTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGtctc                                             <  1:342655/140‑1 (MQ=255)
                                                                                        ttttGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTa                                           <  1:546012/140‑1 (MQ=255)
                                                                                           tGCTTTCTAATATTGCCTGTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAAtt                                        <  2:259431/140‑1 (MQ=255)
                                                                                             gtttCTTATATGGCCGTTAATTCTTTGTCAGTGACGCCGTCTTTTACAAAAGTTAAAAGATATTGATATGTTTCAACAACAATATTAGCTGCTTTTTGAATTAAAGAAATTTTGTCAGCAGCTTTGACGTCTCTAAtttt                                      <  2:63959/139‑1 (MQ=255)
                                                                                                           cTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATGTGTTTTTGGAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAg                        <  1:126999/140‑1 (MQ=255)
                                                                                                               aatgcttcctctttCATGCCTCCTTTTACCAAAGTGAAAATCAATTCGTATCAATCATCAACATTTTGAGTTGCTTTTTGAATTAAAGACATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTCGaaag                    >  2:388304/14‑139 (MQ=255)
                                                                                                                  tCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTCCAGTTTTAGAAATGCt                 >  2:254737/1‑140 (MQ=255)
                                                                                                                   cTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATCTCTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATCAGAAATGCtt                <  1:397977/140‑1 (MQ=255)
                                                                                                                   cTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCtt                >  2:468908/1‑140 (MQ=255)
                                                                                                                        taaGTCATGCCTCCTTTTACAACAGTTAAAATATATTCCTCTGTTTTATACCCAATTTCAGTTGATTAACCAATGAAAACAAGTACACCATCAACTTTGACGTCTCTAATTTTATCTACAGTATTAGCAATGCTTATTaa           >  1:108971/3‑140 (MQ=255)
                                                                                                                         cAGTCATGCCTCCTTGTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAAt          >  1:410876/1‑140 (MQ=255)
                                                                                                                          aGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATg         >  2:365091/1‑140 (MQ=255)
                                                                                                                          aGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTCGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATg         >  2:365120/1‑140 (MQ=255)
                                                                                                                            tCATGCCTCGTTTTACAACAGTTATAATTTATTCATATGTTTCATCAACAATATTTGTGAGCTGGTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGat       <  1:262205/140‑1 (MQ=255)
                                                                                                                             cATGCCTCCTTTTACAACAGTTAATATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATgttg      >  1:351030/1‑137 (MQ=255)
                                                                                                                                 cctcctTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACg  <  1:451786/140‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                        
TCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTGCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACG  >  CP000730/1646057‑1646325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑