Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,744,362 G→A 100% H104Y (CAC→TAC)  apt ← adenine phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,744,3620GA100.0% 180.6 / ‑6.5 64H104Y (CAC→TAC) aptadenine phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  major base A (24/35);  minor base C (2/1);  total (27/37)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTATAAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCC  >  CP000730/1744230‑1744497
                                                                                                                                    |                                                                                                                                       
ttATAAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTCATTGCATCTTTGTACATTGtt                                                                                                                                  >  2:508538/1‑140 (MQ=255)
 tataAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTATACATTGTTa                                                                                                                                 >  1:485917/1‑140 (MQ=255)
  ataAATGCACTACCTACTACTATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTaa                                                                                                                                <  1:118176/140‑1 (MQ=255)
  ataAATGCAATACCTACTACGATCCCGCCTACTTTTTCCCCTAATTTTATTGTTGCTTCAATCGTACCACCAGTAGCTAATAACTCATCTGTACTTAACACCCGTTGCCCTGGCTTCCTTGCATCTTTGTCCATTGTTac                                                                                                                                >  2:500515/1‑139 (MQ=255)
  ataAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTaa                                                                                                                                <  2:132833/140‑1 (MQ=255)
    aaaTGCAATACTTACTACGATCCCGCCTAATTTTTCAACTAATTGTATGGCTGCTTCAATCGTACCCCCAGTAGCTAATAAATCAGCTGTAATTAACACTCGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTaaat                                                                                                                              <  2:494554/140‑2 (MQ=255)
     aaTGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAAc                                                                                                                             <  1:385386/140‑1 (MQ=255)
        gCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGGTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTCCATGGTTaaccacgt                                                                                                                          >  2:548250/1‑134 (MQ=255)
        gCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACAAGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTACAACAtt                                                                                                                          >  2:536600/1‑140 (MQ=255)
          aaTACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACGTTTg                                                                                                                        <  1:374699/140‑1 (MQ=255)
           aTACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGt                                                                                                                       <  1:83589/140‑1 (MQ=255)
           aTACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGt                                                                                                                       <  1:315707/140‑1 (MQ=255)
           aTACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTCTACATTCTTAAAACCCTTGt                                                                                                                       <  2:267290/140‑1 (MQ=255)
              ccTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTAAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTAcc                                                                                                                    >  2:518603/1‑140 (MQ=255)
              ccTACTACGATACCGCCTAATTTTTCAACTAATTGTATGGCTGCTTCAATGTTAGGACCAGTGGCTAGTAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTAAATTTTTACAACAtttttccc                                                                                                                    >  2:130829/1‑135 (MQ=255)
               ctactaCGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCa                                                                                                                   <  1:177389/140‑1 (MQ=255)
                   tacGATACCGTCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTCGTACCATAtt                                                                                                               <  1:398105/140‑1 (MQ=255)
                     cGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCt                                                                                                             <  2:284871/140‑1 (MQ=255)
                     cGATACCGCCTAATTTTTCAACTAAGTTTATTGCTGCCTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCt                                                                                                             <  2:151627/140‑1 (MQ=255)
                      gATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTa                                                                                                            >  2:245255/1‑140 (MQ=255)
                           cgtcTACTTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCa                                                                                                       <  2:11306/137‑1 (MQ=255)
                              cTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATGGCATCTTTGTACATTGTTAACACATTTGTACGATATTCTAGGTCAtcc                                                                                                    >  1:253879/1‑138 (MQ=255)
                                  tttttCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCAt                                                                                                >  1:548136/1‑140 (MQ=255)
                                     ttCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAAc                                                                                             <  2:200686/140‑1 (MQ=255)
                                      tCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATTATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACg                                                                                            <  1:247014/140‑1 (MQ=255)
                                          cgaaTTTTATGGCTGCTGCAGTCGGACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATg                                                                                        >  2:404716/3‑140 (MQ=255)
                                              ttttATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACtt                                                                                    >  2:426784/1‑140 (MQ=255)
                                                    tgctgcTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACCCGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAg                                                                              >  2:518003/1‑140 (MQ=255)
                                                          ttCAATCGTACCACCAGTAGCTAATAAATCCTCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAAtt                                                                        <  2:301045/140‑1 (MQ=255)
                                                          ttCAATCGGACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAAtt                                                                        <  1:422370/140‑1 (MQ=255)
                                                           tCAGTCGTAACATCAGTACCTAATAAATCATCTGTAATTAACACATGTTGAGCTGGTTTAATTGCATCTTTTTATATTGTTAAAACATTTCTACCATATTCAAGGTAATACTCATAACGAATGACTTCACGAGGTAAttt                                                                       <  1:26269/140‑1 (MQ=255)
                                                           tCAATCGTACCACCAGTAGGTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTTTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAAttt                                                                       <  2:501888/140‑1 (MQ=255)
                                                           tCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTTGGTCATACTCATAACGAATGACTTCACGAGGTAAttt                                                                       <  2:500887/140‑1 (MQ=255)
                                                           tCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAAttt                                                                       <  1:502799/140‑1 (MQ=255)
                                                            taaTCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTTTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTc                                                                      <  1:520855/139‑1 (MQ=255)
                                                             aaccgcaccaccAGTAGCCAAGAAATCATCTGTAATTAACACACGTTGACCTGGTTTAACTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTcc                                                                     <  2:424135/134‑1 (MQ=255)
                                                             aaTCGTACCCCCCGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTcc                                                                     <  2:23821/140‑1 (MQ=255)
                                                              agcGTACCACCAGTAGCTAATCAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTccc                                                                    <  2:150138/138‑1 (MQ=255)
                                                              aTCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTccc                                                                    >  2:52857/1‑140 (MQ=255)
                                                                cGTACCACCAGTAGCTAATAGATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGCATGCCTTCACGAGGTAATTTACctt                                                                  >  1:335025/1‑140 (MQ=255)
                                                                cGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACAAATGACTTCACGAGTTAATTTCCctt                                                                  >  1:184701/1‑140 (MQ=255)
                                                                            aGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAg                                                      <  1:204718/140‑1 (MQ=255)
                                                                              cTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGt                                                    >  1:355108/1‑140 (MQ=255)
                                                                                aataaATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGc                                                  >  2:480253/1‑140 (MQ=255)
                                                                                aataaATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGGTAAAACATTTGTACCATATTCTCGGGCATACTCCTAACGAATGAATTCACGAGGTAAGTGCCCTTCTGTTCTAACAgtagc                                                  >  2:351630/1‑136 (MQ=255)
                                                                                    aaTCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAg                                              <  1:187605/140‑1 (MQ=255)
                                                                                      tcatcTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTCGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGGAATTTCCCTTCTTTTCTAACAGGTGCAAAGcc                                            <  2:283214/140‑1 (MQ=255)
                                                                                          cTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTTTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACTAATGACTTCACGAGGTAATTTCCATTCTTTTCTATCAGGTGCAAAGCCAATc                                        >  1:105578/1‑140 (MQ=255)
                                                                                          cTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCaaac                                        >  2:453120/1‑138 (MQ=255)
                                                                                            gTAATTACCACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCACAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATccc                                      <  1:332284/140‑1 (MQ=255)
                                                                                            gTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATccc                                      >  1:57613/1‑140 (MQ=255)
                                                                                            gTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATccc                                      <  1:426784/140‑1 (MQ=255)
                                                                                               aTTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTATCGTGGAAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCAt                                   <  1:429164/140‑1 (MQ=255)
                                                                                                       acGTTGACCTGGTTTAATTGCAGCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTGCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAg                           <  1:173371/140‑1 (MQ=255)
                                                                                                          ttGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGTTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTa                        >  1:19948/1‑140 (MQ=255)
                                                                                                          ttGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTa                        <  1:518003/140‑1 (MQ=255)
                                                                                                            gACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACa                      >  1:350836/1‑140 (MQ=255)
                                                                                                             tccTTGTTTAATTGCAGCTTTGTACATTGGTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGTACCTCCAATCCCCATTGAATAAGCTACAg                     <  2:253242/139‑1 (MQ=255)
                                                                                                             aCCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCAATTGAATAAGTTACAg                     <  2:392556/140‑1 (MQ=255)
                                                                                                             aCCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACTAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAg                     >  1:522686/1‑140 (MQ=255)
                                                                                                                   tttAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTACAAAGCCAAACCCCATTGAATAAGCTCAAGGACAGc               >  1:55666/1‑140 (MQ=255)
                                                                                                                     tAATTGCATCTTTGTCCACTGTTAAAACATTTGTACCATTTTCTAGGTCATACTCATAACGAATGACTTGACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCa             <  2:427166/140‑1 (MQ=255)
                                                                                                                             tCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGATTGACTTCACGTGTTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAAACCCCATTGAATAAGTTACAGGACAGCAAATTATaaa     >  1:251252/1‑140 (MQ=255)
                                                                                                                                ttGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACTAGTTAATTTCCCTTCTTTTCTAACATTTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGcc  <  1:415833/140‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                       
TTATAAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCC  >  CP000730/1744230‑1744497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: