Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,761,889 C→A 100% D388Y (GAT→TAT)  folC ← tetrahydrofolate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,761,8890CA96.2% 98.5 / ‑3.0 37D388Y (GAT→TAT) folCtetrahydrofolate synthase
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base A (18/16);  total (21/16)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CTTCATGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCGG  >  CP000730/1761754‑1762021
                                                                                                                                       |                                                                                                                                    
cttcATGGCTATTTTAGTTTTAGTTTTATAAGGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACGAAGGTATAACCTTGATAATTTTTTATACATTCAACGTAGCCATatact                                                                                                                                  >  2:55662/1‑140 (MQ=255)
  tcATGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTaa                                                                                                                                >  1:274941/1‑140 (MQ=255)
  tcATGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTaa                                                                                                                                >  1:485626/1‑140 (MQ=255)
   tattgCTATTTTATTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAAt                                                                                                                               <  1:503362/136‑1 (MQ=255)
    aTGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATAAAGACTACCGGTAATTACTAATGTATAAACTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAAtt                                                                                                                              >  1:344485/1‑140 (MQ=255)
     tGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTATGAATATACTAATTg                                                                                                                             >  2:194223/1‑140 (MQ=255)
            tttagttttagtTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATAACATTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTATATTtca                                                                                                                      >  1:59316/1‑140 (MQ=255)
               agttttagttttATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTtcatca                                                                                                                   >  2:92897/1‑140 (MQ=255)
               agttttagttttATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTtcatca                                                                                                                   >  2:450585/1‑140 (MQ=255)
                   ttagttttaTAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCAGATACTAATTGTATTTCATCAtttt                                                                                                               <  1:352826/140‑1 (MQ=255)
                               tGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATActaccta                                                                                                   <  1:280700/140‑1 (MQ=255)
                               tGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATActaccta                                                                                                   <  1:407120/140‑1 (MQ=255)
                               tGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATActaccta                                                                                                   <  2:228610/140‑1 (MQ=255)
                                 cTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACa                                                                                                 <  2:511374/140‑1 (MQ=255)
                                 cTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACa                                                                                                 <  1:364391/140‑1 (MQ=255)
                                    cAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTCGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAAtt                                                                                              <  2:275611/140‑1 (MQ=255)
                                         tCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCATTTTTATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATttcttc                                                                                         <  1:181853/140‑1 (MQ=255)
                                                 tGATTTAGCTTCACTTATGAGATACAGACTACCGGTAATTACTACTGTATCACCTTGATAATTTTTTATAAATTAAACGTAGTCATGTCCTAATGGTATTTCATCATTTTCAATACTACCTACAAGTTCTTCTTTTCGTa                                                                                 >  1:345126/1‑140 (MQ=255)
                                                    tttAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCTATACTACCTACAATTACTTCTTTGCGTAACg                                                                              >  1:96925/1‑140 (MQ=255)
                                                     ttAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTAAACTTTCTTCTTTGCTTCACGc                                                                             >  2:310660/1‑140 (MQ=255)
                                                            cACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGa                                                                      >  1:532295/1‑140 (MQ=255)
                                                               ttATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGaaaa                                                                   <  1:441974/140‑1 (MQ=255)
                                                                   gAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTAATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCaa                                                               >  1:44/1‑140 (MQ=255)
                                                                            acgaCCGTTAATTACTGATGTATCCCCTTGATAATTTTTTATTAAATCAACGTAGTCATATATTCATCGTATTTCATCATTTTCAATACTACCTATAATTTCTTCTTTGCGTAACGCTTTCGGAACATTAAATTCAGttt                                                      >  2:204563/4‑139 (MQ=255)
                                                                            aCTACCGGTAATTACTAATGTATCACCTTGATAATTTGTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATGTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTTGGAAAATCAAATTCAGTTg                                                      >  2:143434/1‑140 (MQ=255)
                                                                             cTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGc                                                     <  1:230994/140‑1 (MQ=255)
                                                                              tACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCa                                                    <  1:345931/140‑1 (MQ=255)
                                                                                    tAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCCACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAAc                                              <  1:457019/140‑1 (MQ=255)
                                                                                          ttAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGCAGTGCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATgc                                        <  1:126173/139‑1 (MQ=255)
                                                                                               gTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAAt                                   <  1:205422/140‑1 (MQ=255)
                                                                                                     ccTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGGAAATCATCATTTTCAATACTAACTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACt                             >  1:429032/1‑140 (MQ=255)
                                                                                                         gATAGTTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTaaa                         >  2:335365/1‑140 (MQ=255)
                                                                                                                   tataAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGAAATTAAACTTAAATGTTTGAcct               >  2:319475/1‑139 (MQ=255)
                                                                                                                    ataAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTATTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAGATGTTTCACCAt              >  2:396281/1‑140 (MQ=255)
                                                                                                                      aaaTTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCAtct            >  2:449497/1‑140 (MQ=255)
                                                                                                                              cGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATc    >  1:176537/1‑140 (MQ=255)
                                                                                                                               gTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCg   <  2:128320/140‑1 (MQ=255)
                                                                                                                                tAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTTCGTAACGCTTTCGGAAAATCAAATACAGTTGCATAAAACATATGCGCAATTAAACTTAAATGTTTGACCATCACGTTAATCgg  >  1:233655/1‑140 (MQ=255)
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CTTCATGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAATCGG  >  CP000730/1761754‑1762021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑