Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | CP000730 | 2,279,705 | G→A | 100% | intergenic (+1373/+6659) | mtlD → / ← glmM | mannitol‑1‑phosphate 5‑dehydrogenase/phosphoglucosamine mutase |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | CP000730 | 2,279,705 | 0 | G | A | 100.0% | 149.6 / NA | 53 | intergenic (+1373/+6659) | mtlD/glmM | mannitol‑1‑phosphate 5‑dehydrogenase/phosphoglucosamine mutase |
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (29/24); total (29/24) | |||||||||||
Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
TGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTG > CP000730/2279569‑2279840 | tgtgCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACtg > 2:458515/1‑140 (MQ=255) ttattaGTTGATTCAACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTc > 2:373072/1‑140 (MQ=255) attaGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCAc > 1:446425/1‑140 (MQ=255) ttaGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGCTGTTACTGTTCTTTCAcc > 2:2530/1‑140 (MQ=255) gTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATAAGTTGCTGATGTTACTTTTGTTTCACCTGt > 2:226943/1‑140 (MQ=255) ttGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTTTCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGtt < 1:1024/140‑1 (MQ=255) aaatCTACTCCTGCTTTACCAGTTACAACGTCATCCATTGCCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGc < 2:94212/137‑1 (MQ=255) ttcttcttcTGGTTTACTAGTTACATTGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCg < 2:101435/140‑1 (MQ=255) cTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCa > 1:197696/1‑140 (MQ=255) aCTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGTGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTg < 1:190047/140‑1 (MQ=255) aCTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTg < 1:7503/140‑1 (MQ=255) tagttctttCGTTATCCATTCTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTCTTAGCTTGCGTTTTATCATTTGTATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGgt < 1:41416/132‑1 (MQ=255) tAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGgt > 1:440848/1‑140 (MQ=255) aGTTACATCGTTTTCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGgtg < 2:210424/140‑1 (MQ=255) ttACATGGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGtgtt > 2:392275/1‑140 (MQ=255) aCATCGGTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCCCATCCCTATCATTTGGtgttgt < 2:416183/140‑1 (MQ=255) tCGTTATCCATTGTCCGACTGTTTGATGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCgg > 1:275946/1‑140 (MQ=255) cGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTCGCTTGCGGTCTATAATTTGCATCATCAGTTTTTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGga > 2:265323/1‑140 (MQ=255) tCCATTGTCGTATTGTTTGTTGATGCATCTACACTAGAATTTTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGc < 1:220540/140‑1 (MQ=255) tCCATTGTCGGACTGTTTGTTGATGCATCTACACTATAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGc < 1:482572/140‑1 (MQ=255) tCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCCCTCTTATTTGGTGTTGTCGGGGAAGc > 2:533711/1‑140 (MQ=255) cATTGTCGGACTGTTTGTTGTTGCATCTTCTCTAGTATTGTTATTTGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGt < 1:232796/140‑1 (MQ=255) cATTGTCGGACTGTTTGTAAATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGt > 2:529990/1‑140 (MQ=255) ttGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATAACTATTATTTGGTGTTGTCGGAGAAGCGtgt > 1:184584/1‑138 (MQ=255) tGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCAGTGGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTg < 1:242130/140‑1 (MQ=255) gTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCTGAGAAGCGTCTGc > 1:271094/1‑140 (MQ=255) ggACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGGTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTAGTTGGTGTTGTCGGGGAAGCGTCTGCgtt > 2:293600/1‑140 (MQ=255) aCTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGc < 2:355025/140‑1 (MQ=255) aCTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTACATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGc > 1:144423/1‑140 (MQ=255) tGCATCTACACTAGAATTGTTATTTGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTgtcg < 2:383154/140‑1 (MQ=255) gCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCACTTGCTGATGTTACTGTTGTTTCACCTGTTCCTGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTgtcgt > 2:232436/1‑140 (MQ=255) tCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGactc > 2:178796/1‑140 (MQ=255) taccgtAGTATTGTTATTAGCTTGCGGTTTATCATTTGCGTCATCCGTTGCTGATGTTACTGTTGTTTGCCCTGTTGGCGCATTACTATTATTTGGTGTTGTTGGGGCAGCGTCGGCGTTGCCATTAGCTgtcgtataac < 2:142486/135‑6 (MQ=255) tACACTAGAATTGTTATTAGCTTGCGGTTTATCCTTTGCATCATCAGTTGCTGATGTTACTGCTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAg < 1:371521/140‑1 (MQ=255) tACACTAGAATTGTTATTAGCTTGCGGTTTAGCATTGGCATCTTGAAGGACCTATTTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAg < 1:536897/140‑1 (MQ=255) ggtgcTAGTATTGTGACTAGCCGGCCGTTTATCATTTGCATCATCAGCGCCTGATGCTACTGTTGTTTCCCCTGCTGCCGCATCACTATTATTTTGGGTTGTCGGAGAATCGGCTGCTTTGCCATTCGCCGGTGTCTCAg < 2:213693/136‑1 (MQ=255) aTTGTTATTAGCTTGCGGTTTATCATATGCATCATCAGTTGCTTTTGTTACTGTTGTTTCACCTGTTGCCGAATCACTATTATTTGGTGTTGTTGGGGAAGCGTCTGCGTTGCCATTATCTGTCGTCTTCGGTACGGTAg > 2:329398/1‑140 (MQ=255) ttattaGCTTGCGGTTTATCATGTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTg > 2:4790/1‑140 (MQ=255) tattaGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCGTTGCCATTAGCTGTCGTCTCCGATCCGTTAGGTTGt > 2:326318/1‑140 (MQ=255) tattaGCTGGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTACCTGTCGTCTCAGAAACGTTAGGTTGt > 2:346834/1‑140 (MQ=255) tgCTTGGGGTTGTTCATTTGGTTCATCAGTTTCTTATGTTACTGTTGTTTCACCTGTTGCCGCATCCCTATTATTTGGTGTTTTCGGAGAAGGGTCTGCTTTGCCATTAGTTGTCGTCTCAGATACGTTAGGTTGTCCAg < 2:263045/139‑1 (MQ=255) cGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTAtttt > 1:54800/1‑140 (MQ=255) gTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTCGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCt < 2:375840/140‑1 (MQ=255) tATCGTGTGCATCATCAGTTGCTGATGATACTGTTGTTTCACCTATTGCCGTAACCCGCTACAGCGCGGTGGTCGGTGTGGCGGCTGCTTTGCCGTAGGCGGGCGTCTCAGATACGTGAGGCTGTCCAGTATTTTCTgat > 2:223883/1‑138 (MQ=255) atcatcAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTACGTTGTCCAGTATTTTCTGGTGTTGCATTAg > 2:150726/1‑140 (MQ=255) atcatcAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCGGTCGTCTCAGATACGTTAGGTTGTCCAGTGTTTTCTGGTGTTGCATTAg > 2:172672/1‑140 (MQ=255) tcatcaGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCCCTATTATTTGGTGTTGTCGGAGAAGCGTATGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGc > 1:235235/1‑140 (MQ=255) tcatcaGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTATCTGTCGTCTCAGATACGTTAGATTGTCCAGTATTTTCTGGTGTTGCATTAGc > 2:126095/1‑140 (MQ=255) tcatcaGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCGGGTGTTGCATTacc > 2:265874/1‑138 (MQ=255) atcaGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCAt < 2:54800/140‑1 (MQ=255) caGTTGCTGATGTTACTGTTGTTTCACCTTTTGCCGCATCACTATTATTTGGTTTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCAttt < 1:506664/140‑1 (MQ=255) gATGTTACTTTTTTTTCACCTGTTGCCGCATCACTTTTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGc < 1:415005/140‑1 (MQ=255) tGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTg < 2:446425/140‑1 (MQ=255) | TGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTG > CP000730/2279569‑2279840 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |