Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 35,814 Δ1 bp 31.8% intergenic (+45/+63) USA300HOU_0025 → / ← USA300HOU_0026 hypothetical protein/IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073035,8140A.31.8% 215.7 / 132.1 110intergenic (+45/+63)USA300HOU_0025/USA300HOU_0026hypothetical protein/IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base A (41/30);  new base . (8/27);  total (50/60)
Fisher's exact test for biased strand distribution p-value = 8.59e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.20e-01

Only 100 of 112 total aligned reads displayed.

TATGCAAATTATTCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATA  >  CP000730/35674‑35954
                                                                                                                                              |                                                                                                                                             
  tGCAAATTATTCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGa                                                                                                                                             >  1:606278/1‑141 (MQ=255)
    cAAATTATTCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAg                                                                                                                                           >  1:573131/1‑141 (MQ=255)
         tattCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCt                                                                                                                                      >  2:557842/1‑141 (MQ=255)
                     aaaGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTaa                                                                                                                          >  1:90232/1‑141 (MQ=255)
                       aGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAGGGTTCTGTTGCAAAGTaaaa                                                                                                                        >  2:186880/1‑141 (MQ=255)
                              gatgatCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAAtata                                                                                                                 <  1:585347/141‑1 (MQ=255)
                              gatgatCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGAAAAAAGAAAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAAtata                                                                                                                 <  2:583935/141‑1 (MQ=255)
                               atgatCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAg                                                                                                                >  1:273787/1‑141 (MQ=255)
                                 gatCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAGA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGTAAAGTAAAAAAATATAGCt                                                                                                              <  1:461456/141‑1 (MQ=255)
                                  atCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTa                                                                                                             >  2:70965/1‑141 (MQ=255)
                                       aTTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCAc                                                                                                        <  1:318002/141‑1 (MQ=255)
                                        ttGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGATTTTGGCGAAAAATGATTACTTGAACAAGAACTAAAA‑‑TAAAATTGTGAGCTAATAAAAATAGAAGGTTCTGTTGCAAAGTACAAAAATATAGCTAACCACt                                                                                                       >  2:128784/1‑141 (MQ=255)
                                          gACAAAGTATTTAATGTAGCAGTGGTGCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTaa                                                                                                     >  2:165874/1‑141 (MQ=39)
                                          gACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAACAATGATTACTTGAACAAGCGCCAACAGCTGAAATTGGACAAAGGAAAATACAAGGGACTGTTGCAAAGTAAAAAAAGATAGCTAACCACaaa                                                                                                     >  2:734231/1‑141 (MQ=255)
                                           aCAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTGTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAGAAAATATAGCTAACCACTAAt                                                                                                    >  1:187466/1‑141 (MQ=255)
                                              aaGTATTTAATGTAGAAGCTATTGAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTa                                                                                                 >  2:548819/1‑141 (MQ=255)
                                              aaGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTCAtttt                                                                                                 >  2:226169/1‑140 (MQ=255)
                                              aaGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTa                                                                                                 >  1:534413/1‑141 (MQ=255)
                                              aaGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACAACTAATTTa                                                                                                 >  1:431520/1‑141 (MQ=255)
                                                gTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATc                                                                                               >  1:187510/1‑141 (MQ=255)
                                                  aTTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCAt                                                                                             <  1:414221/141‑1 (MQ=255)
                                                   tttAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAGAAATGATTACTTGAACAAGAGCTAACA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTGTGTTGCAACGTAAAAAAATATAGATAACCAGTAATTTATTATg                                                                                            >  2:617607/1‑141 (MQ=255)
                                                   tttAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATg                                                                                            >  1:374363/1‑141 (MQ=255)
                                                     tAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTAGCATGTc                                                                                          <  2:424938/141‑1 (MQ=255)
                                                      aaTGTAGAAGCTATTGAGTATAAAGCTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCa                                                                                         >  1:386021/1‑141 (MQ=255)
                                                      aaTGGAGAAGCTAATCAGTATAAAGGTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTAATAAAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCa                                                                                         <  2:417546/141‑1 (MQ=37)
                                                            gAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGCGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTCTTGCAATGTAAAAAAATATGGCTAACCACTAATGTATCATGACAGCGTTc                                                                                   >  1:78298/1‑141 (MQ=255)
                                                              aGCTACTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCGGTTGCAAAGTAAAAAAATATAGCTAACCTCTAATTTATCATGTCAGTGTTCGc                                                                                 <  1:145417/141‑1 (MQ=255)
                                                               gCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCt                                                                                >  1:592981/1‑141 (MQ=255)
                                                                  aTTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCAAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTACTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATATAAGTGTTCACATaa                                                                             >  2:713768/1‑141 (MQ=37)
                                                                     cAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACtt                                                                          >  2:403176/1‑141 (MQ=255)
                                                                      aGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTg                                                                         >  1:631679/1‑141 (MQ=255)
                                                                       gTATAAAGTTTTTGGCGAAAAATGATTAGTTGAACAAGAGCTAATA‑‑GAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGc                                                                        <  2:113515/141‑1 (MQ=255)
                                                                                         aaaaTGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTc                                                     <  1:404544/141‑1 (MQ=255)
                                                                                           aaTGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGt                                                   <  2:744828/141‑1 (MQ=255)
                                                                                            aTGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTg                                                  <  1:125692/141‑1 (MQ=255)
                                                                                             tGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTgg                                                 >  1:238577/1‑141 (MQ=255)
                                                                                              gATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGc                                                >  1:367499/1‑141 (MQ=255)
                                                                                              gATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGc                                                >  1:117231/1‑141 (MQ=255)
                                                                                              gATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATACTAATTTCTTGGc                                                >  1:285700/1‑141 (MQ=255)
                                                                                               aTTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCa                                               >  1:147266/1‑141 (MQ=255)
                                                                                                   cTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGc                                           <  1:283214/141‑1 (MQ=255)
                                                                                                   cTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGc                                           <  1:161676/141‑1 (MQ=255)
                                                                                                   attaaaaaaGAGCTAAAA‑‑TAAAATTGTTATCTAAAAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGc                                           <  2:534380/134‑1 (MQ=255)
                                                                                                           agagCTAAAA‑‑TAAAATTGTGGTCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATcc                                   >  2:14839/1‑141 (MQ=255)
                                                                                                            gagCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCg                                  <  2:210447/141‑1 (MQ=255)
                                                                                                            gagCTAAAA‑‑TAAAACTGGGATCTAATAAAAATCGAAGGTTCTGTTGCAACGACAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACGTGCTAGCATGATGCTACTTACGTGGccgtgcgagactccg                                  >  2:172539/1‑127 (MQ=17)
                                                                                                             agCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAGAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAAGTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGt                                 >  2:336004/1‑141 (MQ=255)
                                                                                                              gCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTGGCATGCTGCCCAATCTCTGGCATGGCGAAAATCCGTa                                <  1:184987/141‑1 (MQ=18)
                                                                                                                  aaa‑‑taaaatTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGCTc                            >  1:170029/1‑141 (MQ=255)
                                                                                                                  aaa‑‑taaaatTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATc                            >  1:94181/1‑141 (MQ=255)
                                                                                                                      acaaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCCGaa                        <  1:245252/127‑1 (MQ=11)
                                                                                                                       taaaatTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATACCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGa                         >  1:146200/1‑141 (MQ=255)
                                                                                                                        aaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAaga                      <  2:325815/129‑1 (MQ=11)
                                                                                                                         aaatTGTTATCTAATAAAAATAGAAGTCGCTGTGGCAAAGTAACAAAATATAGCTAACCACTAATTTATCATGTCAGTGTCCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAag                       <  2:85341/141‑1 (MQ=14)
                                                                                                                         aaatTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAag                       <  1:669591/141‑1 (MQ=255)
                                                                                                                         aaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAagag                     <  1:741271/130‑1 (MQ=11)
                                                                                                                           atTGTGATCTAATAAAAATAGAAGGGTTTGTTGAAAAGAAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAagag                     <  2:273787/141‑1 (MQ=255)
                                                                                                                           agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                   <  1:102241/132‑1 (MQ=11)
                                                                                                                           agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                   <  2:461944/132‑1 (MQ=11)
                                                                                                                           agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                   <  1:452464/132‑1 (MQ=11)
                                                                                                                           agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                   <  1:104734/132‑1 (MQ=11)
                                                                                                                           agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                   <  1:640564/132‑1 (MQ=11)
                                                                                                                           agagtagttgaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                   <  2:374421/131‑1 (MQ=11)
                                                                                                                            gagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                  <  2:240880/133‑1 (MQ=11)
                                                                                                                            gagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                  <  1:98840/133‑1 (MQ=11)
                                                                                                                            gagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGATAATCACTAATATATCCTGTCAGTGTTCGTTTAACTTACTAGCATACTGCTACTTTCGGGGCATGGCGAAAATCCGTAGATATGAAGAAAcc                  >  2:747799/9‑141 (MQ=2)
                                                                                                                             tgtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                   >  2:306295/1‑141 (MQ=255)
                                                                                                                              gtgATCTAATACAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                  <  1:170459/141‑1 (MQ=255)
                                                                                                                              gtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                  <  1:359196/141‑1 (MQ=255)
                                                                                                                              gtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                >  2:529666/7‑141 (MQ=11)
                                                                                                                              gtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGATAATCACTAATATATAATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                >  1:108390/7‑141 (MQ=11)
                                                                                                                              gtattttaataaAAACGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCGAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                <  1:23048/135‑1 (MQ=11)
                                                                                                                              gtattttaataaAAACGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACATGCTAGCATGATGTTAATTTCGTGGCATGGCGAAAATTCGTAGATCTGAAGAGACCTg                <  1:466066/135‑1 (MQ=11)
                                                                                                                               tattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAACATCCGTAGATCTGAAGAGACCTGc               >  2:329494/6‑141 (MQ=11)
                                                                                                                               tattttaataaAAATGATGGTTCTGCAGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc               <  1:587785/136‑1 (MQ=11)
                                                                                                                               gattttaataaAAACGATGGGTCTTTTGCAAAGTAAAAAACATAGCTAATCACGAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc               <  1:347449/136‑1 (MQ=11)
                                                                                                                                gATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAACTCAGTAGATCTAAAGAGACCTg                >  2:720003/1‑141 (MQ=255)
                                                                                                                                gATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGATGAGACCTg                >  2:525534/1‑141 (MQ=255)
                                                                                                                                gATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                >  1:634764/1‑141 (MQ=255)
                                                                                                                                attttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAATTCCGTAGATCTGAAGAGACCTGCg              <  2:589830/137‑1 (MQ=11)
                                                                                                                                 ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg             <  1:71200/138‑1 (MQ=11)
                                                                                                                                 ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg             >  2:326033/4‑141 (MQ=11)
                                                                                                                                 ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCGAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg             >  1:748206/4‑141 (MQ=11)
                                                                                                                                 aTCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc               <  1:130404/141‑1 (MQ=255)
                                                                                                                                  ttaaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCCCTCATACATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt            <  1:260537/138‑1 (MQ=11)
                                                                                                                                  tCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg              <  1:19658/141‑1 (MQ=255)
                                                                                                                                   ttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCTGTAGATCTGAAGAGACCTGCGGtt           <  1:717824/140‑1 (MQ=11)
                                                                                                                                   ttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGtt           <  1:399858/140‑1 (MQ=11)
                                                                                                                                   ttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGtt           <  2:41180/140‑1 (MQ=11)
                                                                                                                                   ttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCAGtt           <  1:551527/140‑1 (MQ=11)
                                                                                                                                     aataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAGCCCGTAGATCTGAAGAGACCTGCGGTTCt         >  2:77445/1‑141 (MQ=11)
                                                                                                                                     aataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCt         <  2:541797/141‑1 (MQ=11)
                                                                                                                                     aataaAAATGATGGTTCTGTCGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCt         <  2:578283/141‑1 (MQ=11)
                                                                                                                                     aataaAAAGAGAAGGTTATGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGtt           <  2:224697/141‑1 (MQ=255)
                                                                                                                                      ataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCtt        <  2:624017/141‑1 (MQ=11)
                                                                                                                                           aaTAGAAGGCTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCttttt     <  1:239082/141‑1 (MQ=21)
                                                                                                                                            aTAGACGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACGTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTta    <  2:656717/141‑1 (MQ=17)
                                                                                                                                             tAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtat   <  1:165874/141‑1 (MQ=32)
                                                                                                                                              aGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTATCGTAACGTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtata  <  1:557842/141‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                             
TATGCAAATTATTCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATA  >  CP000730/35674‑35954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: