BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,929,842	Δ1 bp	100%	intergenic (‑925/‑243)	USA300HOU_1794 ← / → USA300HOU_1796	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,929,842	0	T	.	59.9%	130.2 / inf	187	intergenic (‑925/‑243)	USA300HOU_1794/USA300HOU_1796	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (35/39); new base . (59/53); total (95/92)
Fisher's exact test for biased strand distribution p-value = 5.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 191 total aligned reads displayed.

CTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCA  >  CP000730/1929704‑1929981

  cATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGAGATAATAGGAGAGACGCCTATCTCTCAGGCGTCAATTTAGAc                                                                                                                                                >  1:582040/1‑141 (MQ=255)

      gAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCag                                                                                                                                            <  1:579322/141‑1 (MQ=255)

       aaTTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGCTATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCaga                                                                                                                                           <  2:491911/141‑1 (MQ=255)

        aTTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAATTGACTGTGCTTGTAGATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCagag                                                                                                                                          <  1:729192/141‑1 (MQ=255)

        aTTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAAACATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGAAATAAGAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCaaag                                                                                                                                          >  2:67412/1‑141 (MQ=255)

          ttGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCagagag                                                                                                                                        <  2:659781/141‑1 (MQ=255)

          ttGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCagagag                                                                                                                                        <  2:449417/141‑1 (MQ=255)

             tCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAgaggag                                                                                                                                     <  1:194253/141‑1 (MQ=255)

               cAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATGATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATGTAATAGTAGAGTCGCCTATCTCTCAGGCGTGAATTTAGACGCAGAGaggaggg                                                                                                                                   >  1:415104/1‑140 (MQ=255)

               cAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGCCGCTTCTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGgt                                                                                                                                   <  1:750339/141‑1 (MQ=255)

               cAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGgt                                                                                                                                   <  1:24811/141‑1 (MQ=255)

                   tAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGta‑t                                                                                                                              <  1:511575/141‑1 (MQ=255)

                              gATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGAGGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑t                                                                                                              >  2:286778/1‑141 (MQ=255)

                                   aaTCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTAttt                                                                                                         <  1:622391/141‑1 (MQ=255)

                                   aaTCTATCAGCTAGAATCATATAACTTATCAGAAGCAAATGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTAttt                                                                                                         >  2:576517/1‑141 (MQ=255)

                                   aaTCTATCAGCTAGAATCACATAACTTATCAGAAGCAAGTGACTGTGCTTGTAACTTCGCTCTGCATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTAttt                                                                                                         <  1:365724/141‑1 (MQ=255)

                                     tCTATCAGCTAGAATCATATAATTTAGCAGAAGCAAGTGACTGTGCTTTTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTc                                                                                                       <  2:171522/141‑1 (MQ=255)

                                                             tATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGtcatc                                                                              <  1:8121/141‑1 (MQ=255)

                                                                   tcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCt                                                                       <  1:715230/128‑1 (MQ=255)

                                                                   tcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTTGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCt                                                                       >  1:377026/14‑141 (MQ=255)

                                                                   gcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCt                                                                       <  1:87935/128‑1 (MQ=255)

                                                                   aaGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGc                                                                        <  1:652195/141‑1 (MQ=255)

                                                                   aaGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATATCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGtcagcctttga                                                                        >  2:19820/1‑133 (MQ=255)

                                                                     ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCtgt                                                                     <  1:62742/130‑1 (MQ=255)

                                                                     ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCtgt                                                                     <  1:63586/130‑1 (MQ=255)

                                                                      gaagtagatatgCTTGTTTATTCACAGATGATATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGATGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                                                    <  2:725921/131‑1 (MQ=255)

                                                                      gaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                                                    <  2:379441/131‑1 (MQ=255)

                                                                      gaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                                                    <  2:562541/131‑1 (MQ=255)

                                                                       aagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                                                   >  1:434977/10‑141 (MQ=255)

                                                                       aagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                                                   <  1:695351/132‑1 (MQ=255)

                                                                        agtcgatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑T‑TTGTGAA‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGct                                                                  <  2:498252/133‑2 (MQ=255)

                                                                        agtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGGGCA‑TTACT‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCCCCAGTCATCAGTGGCTGTGct                                                                  >  2:74521/9‑140 (MQ=255)

                                                                        aGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                                                   >  2:233191/1‑141 (MQ=255)

                                                                          tagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgt                                                                <  1:288542/135‑4 (MQ=255)

                                                                          tGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCa                                                                 >  2:756786/1‑141 (MQ=255)

                                                                           gACTGCGTTTGTATACTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTTGTCGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAt                                                                <  1:1077/141‑1 (MQ=255)

                                                                           gACCGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATGTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCCTCAGTGGCTGTGCCAt                                                                <  2:702695/141‑1 (MQ=255)

                                                                            gatttgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                                                              <  1:48652/137‑6 (MQ=255)

                                                                            gatatgCTTTTAAATTCACAGATGGTATAATAATAGAGCCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                                                              <  1:272892/137‑6 (MQ=255)

                                                                            gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                                                              >  1:245916/5‑136 (MQ=255)

                                                                            gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                                                              <  1:396443/137‑6 (MQ=255)

                                                                            gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACAAGTCATCAGTGGCTGTGctgttg                                                              >  1:11691/5‑136 (MQ=255)

                                                                              tgtgCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTGC‑GTTCCCATCATAGCACCAGTCATCTGTGGCTGTGCCAtttc                                                             >  2:331535/1‑139 (MQ=255)

                                                                              tgtgCTTGTATATTTGCCGATGATATAATAGTAAAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGc                                                             >  1:350511/1‑141 (MQ=255)

                                                                              tggccTTGTATATTTGCCCATGATATAATAGTAGTGTCGCCTATCGCTCAGGCGTCAATTTAGACGTAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCATATCATAGCACCAGTCATCAGTGGCTGTGCCATTGc                                                             <  2:206841/137‑1 (MQ=255)

                                                                               atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGt                                                           <  2:661469/140‑1 (MQ=255)

                                                                               atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGt                                                           >  2:317567/2‑141 (MQ=255)

                                                                                 gCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtat                                                         <  1:704492/141‑3 (MQ=255)

                                                                                  cTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatt                                                        <  1:416547/141‑4 (MQ=255)

                                                                                  cTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatt                                                        <  2:470088/141‑4 (MQ=255)

                                                                                   ttGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGtttt                                                        <  2:236979/141‑1 (MQ=255)

                                                                                    tGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattat                                                      >  1:762306/1‑139 (MQ=255)

                                                                                    tGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattat                                                      <  2:685505/141‑6 (MQ=255)

                                                                                     gtatattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTTGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattata                                                     >  2:255734/12‑138 (MQ=255)

                                                                                       atattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatact                                                   >  2:452646/10‑136 (MQ=255)

                                                                                          ttcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAt                                                <  2:641712/135‑1 (MQ=255)

                                                                                          ttcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATCCTTAt                                                >  2:116941/7‑141 (MQ=255)

                                                                                           ttGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCCGTGCCATTGCGTTTTTTTCTTAt                                                >  2:295084/1‑141 (MQ=255)

                                                                                           tcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAtt                                               <  1:588051/136‑1 (MQ=255)

                                                                                            tGCCGATGATATAATAGAAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACGAGGCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTAtt                                               >  1:244006/1‑141 (MQ=255)

                                                                                            cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACTAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg                                              >  1:46857/5‑141 (MQ=255)

                                                                                              ccGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTgg                                             >  1:666752/1‑141 (MQ=255)

                                                                                              cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc                                            >  1:493152/3‑141 (MQ=255)

                                                                                               agatggtATAATAATCGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGTAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACGAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGACt                                           >  2:214743/2‑141 (MQ=255)

                                                                                                  tggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGGGGCGGTCCTGTTGCGTATTATACTTATTGGCTTAg                                        >  2:61134/4‑141 (MQ=255)

                                                                                                  aggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTTGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag                                        <  2:690362/138‑4 (MQ=255)

                                                                                                    gtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGTGAGTTGGGGCA‑TTA‑C‑TTTGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttatta                                      >  1:720412/2‑136 (MQ=255)

                                                                                                     tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGGGGAGGTGCATTCC‑T‑T‑TGCTA‑A‑TCATTTTCACATCGCCGGA‑AGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                     >  1:177596/1‑140 (MQ=255)

                                                                                                     tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                     <  1:309208/141‑2 (MQ=255)

                                                                                                     tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                     <  1:642279/141‑2 (MQ=255)

                                                                                                       taataaTGGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaac                                   <  2:251347/141‑4 (MQ=255)

                                                                                                       taataaTAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaac                                   <  2:657295/141‑4 (MQ=255)

                                                                                                        aataatAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACg                                  >  2:173057/1‑141 (MQ=255)

                                                                                                           aatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaa                               <  1:323271/139‑1 (MQ=255)

                                                                                                           aatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaa                               <  1:702817/139‑1 (MQ=255)

                                                                                                            gtagAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCaa                               <  1:103884/141‑1 (MQ=255)

                                                                                                            atagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaac                              <  1:230485/140‑2 (MQ=255)

                                                                                                              agagTTCCCTATCGCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGCCGCAATa                             <  2:515252/141‑1 (MQ=255)

                                                                                                              agagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATGCTTATTGGCTTAGTAAACGCaacaa                            <  1:98392/141‑4 (MQ=255)

                                                                                                              agagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAAAGCaacaa                            >  2:283390/1‑138 (MQ=255)

                                                                                                              agagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaa                            >  1:13729/1‑138 (MQ=255)

                                                                                                              agagTCGCCTATCTCTCAGGAGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaa                            >  1:466623/1‑138 (MQ=255)

                                                                                                               gagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaa                           <  2:521906/141‑5 (MQ=255)

                                                                                                                agTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACa                           >  1:132538/1‑141 (MQ=255)

                                                                                                                 gTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAC‑G‑C‑T‑CTG‑CGTTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAACTAGACGCAATACaa                          <  1:402258/141‑1 (MQ=255)

                                                                                                                 gTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACaa                          <  1:193340/141‑1 (MQ=255)

                                                                                                                   cGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataat                       >  2:149529/1‑133 (MQ=255)

                                                                                                                    gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                      >  2:596056/1‑132 (MQ=255)

                                                                                                                    gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                      <  1:317320/141‑10 (MQ=255)

                                                                                                                    gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                      <  2:409843/141‑10 (MQ=255)

                                                                                                                     ccTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATa                      >  2:263200/1‑141 (MQ=255)

                                                                                                                     ccTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatta                     >  2:184971/1‑131 (MQ=255)

                                                                                                                      cTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTTAGTAGATGCAATACAAAATAg                     >  2:712681/1‑141 (MQ=255)

                                                                                                                          ctatctggggTCCATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGa                 <  2:269466/132‑1 (MQ=255)

                                                                                                                             tcAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACat              <  2:36661/141‑1 (MQ=255)

                                                                                                                              cAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACata             >  1:259031/1‑141 (MQ=255)

                                                                                                                                 gCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAg          >  1:689926/1‑141 (MQ=255)

                                                                                                                                      aaTTTAGACGCAGATAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGTCGCa     <  1:547667/141‑1 (MQ=255)

                                                                                                                                         ttAGACGCAGAGGGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCa  <  1:146770/141‑1 (MQ=255)

                                                                                                                                         ttAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCa  <  1:742979/141‑1 (MQ=255)

CTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCA  >  CP000730/1929704‑1929981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`