BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,929,843	Δ1 bp	58.6%	intergenic (‑926/‑242)	USA300HOU_1794 ← / → USA300HOU_1796	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,929,843	0	A	.	58.6%	110.2 / inf	191	intergenic (‑926/‑242)	USA300HOU_1794/USA300HOU_1796	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (36/40); new base . (59/53); total (96/95)
Fisher's exact test for biased strand distribution p-value = 5.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 193 total aligned reads displayed.

CTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAAT  >  CP000730/1929704‑1929983

ctcATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAg                                                                                                                                                    >  2:622391/1‑141 (MQ=255)

   aTAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCGTATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAAAATCGCCTATCTCTCAGGCGTCAATTAAgctg                                                                                                                                                 >  2:233930/1‑138 (MQ=255)

      gAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCag                                                                                                                                              <  1:579322/141‑1 (MQ=255)

      gAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCag                                                                                                                                              <  2:204198/141‑1 (MQ=255)

       aaTTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGCTATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCaga                                                                                                                                             <  2:491911/141‑1 (MQ=255)

        aTTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAAACATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGAAATAAGAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCaaag                                                                                                                                            >  2:67412/1‑141 (MQ=255)

          ttGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCagagag                                                                                                                                          <  2:659781/141‑1 (MQ=255)

             tCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAgaggag                                                                                                                                       <  1:194253/141‑1 (MQ=255)

               cAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATGATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATGTAATAGTAGAGTCGCCTATCTCTCAGGCGTGAATTTAGACGCAGAGaggaggg                                                                                                                                     >  1:415104/1‑140 (MQ=255)

                   tAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGta‑t                                                                                                                                <  1:759823/141‑1 (MQ=255)

                              gATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGAGGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑t                                                                                                                >  2:286778/1‑141 (MQ=255)

                                   aaTCTATCAGCTAGAATCATATAACTTATCAGAAGCAAATGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTAttt                                                                                                           >  2:576517/1‑141 (MQ=255)

                                     tCTATCAGCTAGAATCATATAATTTAGCAGAAGCAAGTGACTGTGCTTTTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTc                                                                                                         <  2:171522/141‑1 (MQ=255)

                                            gCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTcaca                                                                                                 >  2:242692/1‑141 (MQ=255)

                                                       ataACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATACTCGCTAAGGCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCa                                                                                      <  1:556458/141‑1 (MQ=255)

                                                                  gAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTgg                                                                           >  1:36661/1‑141 (MQ=255)

                                                                  atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGCCATCAGCGGc                                                                          >  2:583412/15‑141 (MQ=255)

                                                                  atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCACCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGc                                                                          >  1:508949/15‑141 (MQ=255)

                                                                   tcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTTGCTA‑A‑TCATTTTC‑GTTCCCATCATAGCACCAGTCATCAGTGGcg                                                                         >  2:328926/14‑140 (MQ=255)

                                                                    cggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCtg                                                                        >  2:400108/13‑141 (MQ=255)

                                                                      gaagtagatatgCTTGTTTATTCACAGATGATATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGATGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                                                      <  2:725921/131‑1 (MQ=255)

                                                                      gaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                                                      <  2:562541/131‑1 (MQ=255)

                                                                       aagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                                                     <  1:695351/132‑1 (MQ=255)

                                                                       aagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                                                     >  1:434977/10‑141 (MQ=255)

                                                                        agtcgatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑T‑TTGTGAA‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGct                                                                    <  2:498252/133‑2 (MQ=255)

                                                                        agtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGGGCA‑TTACT‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCCCCAGTCATCAGTGGCTGTGct                                                                    >  2:74521/9‑140 (MQ=255)

                                                                          tagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgt                                                                  <  1:7058/135‑4 (MQ=255)

                                                                          tagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgt                                                                  <  1:288542/135‑4 (MQ=255)

                                                                          tGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCa                                                                   >  2:756786/1‑141 (MQ=255)

                                                                           gACTGCGTTTGTATACTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTTGTCGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAt                                                                  <  1:1077/141‑1 (MQ=255)

                                                                           gACCGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATGTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCCTCAGTGGCTGTGCCAt                                                                  <  2:702695/141‑1 (MQ=255)

                                                                            gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                                                                >  1:245916/5‑136 (MQ=255)

                                                                            gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                                                                >  1:257151/5‑136 (MQ=255)

                                                                            gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACAAGTCATCAGTGGCTGTGctgttg                                                                >  1:11691/5‑136 (MQ=255)

                                                                            aatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                                                                <  1:727175/137‑6 (MQ=255)

                                                                               atgCTTGTATATTCACAGATGGTATCATCATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGt                                                             >  1:304451/2‑141 (MQ=255)

                                                                               atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGt                                                             <  2:661469/140‑1 (MQ=255)

                                                                                  cTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatt                                                          <  2:470088/141‑4 (MQ=255)

                                                                                  cTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatt                                                          <  1:416547/141‑4 (MQ=255)

                                                                                    tGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattat                                                        <  2:685505/141‑6 (MQ=255)

                                                                                      tataTTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatac                                                      <  1:384396/141‑8 (MQ=255)

                                                                                       atattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatact                                                     >  2:452646/10‑136 (MQ=255)

                                                                                        tatgcggcGATGATATAATAGTAGAGCCGCCTATCTCTCAGGTGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCt                                                     <  1:94473/134‑1 (MQ=255)

                                                                                        aattcacagatggtAAAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACtt                                                    <  2:357554/133‑1 (MQ=255)

                                                                                          ttcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAt                                                  >  2:172752/7‑141 (MQ=255)

                                                                                           ttGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCCGTGCCATTGCGTTTTTTTCTTAt                                                  >  2:295084/1‑141 (MQ=255)

                                                                                            tGCCGATGATATAATAGAAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACGAGGCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTAtt                                                 >  1:244006/1‑141 (MQ=255)

                                                                                            cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg                                                >  1:744835/5‑141 (MQ=255)

                                                                                            cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACTAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg                                                >  1:46857/5‑141 (MQ=255)

                                                                                            cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACTAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg                                                >  1:26139/5‑141 (MQ=255)

                                                                                            cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg                                                >  2:254977/5‑141 (MQ=255)

                                                                                            cacagatggtATAATAATAAAGTCGCCTATCTCTCAGGCGTCACTT‑‑GACGAAGGGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTG‑GCTCACAGCATAGCCCCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAttt                                                >  2:229468/5‑140 (MQ=255)

                                                                                              cagatggtATAATAATATAGTGGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCAGCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc                                              <  2:183178/139‑1 (MQ=255)

                                                                                                 atggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtta                                           <  1:622819/141‑3 (MQ=255)

                                                                                                  aggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTTGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag                                          <  2:690362/138‑4 (MQ=255)

                                                                                                   ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGcatagt                                         >  1:586731/3‑136 (MQ=255)

                                                                                                    gtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttcgta                                        >  2:210700/2‑136 (MQ=255)

                                                                                                     tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGGGGAGGTGCATTCC‑T‑T‑TGCTA‑A‑TCATTTTCACATCGCCGGA‑AGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                       >  1:177596/1‑140 (MQ=255)

                                                                                                       taataaTAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaac                                     <  2:657295/141‑4 (MQ=255)

                                                                                                       taataaTAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAAc                                     >  2:300211/1‑141 (MQ=255)

                                                                                                        aataatAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACg                                    >  1:81441/1‑141 (MQ=255)

                                                                                                         ataatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                   <  1:608995/137‑1 (MQ=255)

                                                                                                         ataCTAGAGTCGCCCACTTCTCACGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                   <  2:597991/141‑1 (MQ=255)

                                                                                                          taatagAGTCGCCTATCTCTCGGGCGGCAATT‑‑GACGAAGCGAGAAGGAATAGTTC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa                                  <  1:362513/138‑1 (MQ=255)

                                                                                                           aatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaa                                 <  1:702817/139‑1 (MQ=255)

                                                                                                            gtagAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCaa                                 <  1:103884/141‑1 (MQ=255)

                                                                                                            atagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaac                                <  2:371298/140‑2 (MQ=255)

                                                                                                            atagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaac                                <  1:230485/140‑2 (MQ=255)

                                                                                                            atagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑TGCTA‑A‑TAATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaac                                >  2:195353/2‑140 (MQ=255)

                                                                                                            atagAGTCGCCTAGCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTACACTTATTGGCTTAGTAAACGCaac                                <  1:492174/140‑2 (MQ=255)

                                                                                                              agagTTCCCTATCGCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGCCGCAATa                               <  2:515252/141‑1 (MQ=255)

                                                                                                              agagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAAAGCaacaa                              >  2:283390/1‑138 (MQ=255)

                                                                                                              agagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaa                              >  1:13729/1‑138 (MQ=255)

                                                                                                              agagTCGCCTATCTCTCAGGAGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaa                              >  1:466623/1‑138 (MQ=255)

                                                                                                               gagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaa                             <  2:521906/141‑5 (MQ=255)

                                                                                                               gagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaa                             >  1:719709/1‑137 (MQ=255)

                                                                                                                agTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACa                             >  1:132538/1‑141 (MQ=255)

                                                                                                                 gTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAC‑G‑C‑T‑CTG‑CGTTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAACTAGACGCAATACaa                            <  1:402258/141‑1 (MQ=255)

                                                                                                                 gTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACaa                            <  1:193340/141‑1 (MQ=255)

                                                                                                                 gTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaata                           >  1:742323/1‑135 (MQ=255)

                                                                                                                  tCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataa                          >  1:653965/1‑134 (MQ=255)

                                                                                                                  gcGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACaaa                           <  1:79128/140‑1 (MQ=255)

                                                                                                                   cGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataat                         >  2:149529/1‑133 (MQ=255)

                                                                                                                   cGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataat                         >  1:645293/1‑133 (MQ=255)

                                                                                                                    gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                        >  2:412835/1‑132 (MQ=255)

                                                                                                                     ccTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATa                        >  2:263200/1‑141 (MQ=255)

                                                                                                                     ccTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTGTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATa                        >  1:639477/1‑141 (MQ=255)

                                                                                                                      cTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTTAGTAGATGCAATACAAAATAg                       >  2:712681/1‑141 (MQ=255)

                                                                                                                      cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                      <  1:627713/141‑12 (MQ=255)

                                                                                                                      cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑G‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                      >  1:32603/1‑130 (MQ=255)

                                                                                                                       tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaagtaattaat                     >  1:286993/1‑129 (MQ=255)

                                                                                                                          ctatctggggTCCATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGa                   <  2:269466/132‑1 (MQ=255)

                                                                                                                              cAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGAccga               >  1:322778/1‑138 (MQ=255)

                                                                                                                              cAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACata               <  1:46988/141‑1 (MQ=255)

                                                                                                                              cAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑C‑TCATTTTA‑GTTCACATCCTAGCACCAGTCATCAGTGGCTGTGCTGTTCCGTATTATACTTATTGGCTTAGTAAACGCAACACATAAttaataggcgac              >  2:111110/1‑130 (MQ=255)

                                                                                                                                 gCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAg            >  1:689926/1‑141 (MQ=255)

                                                                                                                                     cAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGc        <  1:668149/141‑1 (MQ=255)

                                                                                                                                     cAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATACGTGACATATAGCCGc        >  1:517510/1‑141 (MQ=255)

                                                                                                                                         ttAGACGCAGAGGGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCa    <  1:146770/141‑1 (MQ=255)

                                                                                                                                          tAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGccacaa   >  1:3115/1‑136 (MQ=255)

CTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAAT  >  CP000730/1929704‑1929983

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`