Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 36,087 A→G 61.6% C155C (TGT→TGC)  USA300HOU_0026 ← IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073036,0870AG61.6% 587.3 / inf 811C155C (TGT→TGC) USA300HOU_0026IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base A (150/162);  new base G (265/230);  total (417/394)
Fisher's exact test for biased strand distribution p-value = 1.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

Only 100 of 811 total aligned reads displayed.

TTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACG‑GTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTA  >  CP000730/35948‑36225
                                                                                                                                             |                                                                                                                                          
   tatataGAGCGTAAATACATTCAATACCTTTTAAAGTAT‑TCTTTGGCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACG‑GTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGt                                                                                                                                        <  2:34321/141‑1 (MQ=255)
       taGAGCGTCAACACCATAAATACCTTTTGAAGTAT‑TCTCTGGTGTATTTATACTTTGATACCTTGTCTTTCTTACTTTAATATGACG‑GTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAgg                                                                                                                                    <  1:392757/141‑1 (MQ=14)
       taGAGCGTAAATACACTCAATACGTTTT‑ACGTCTGGCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACG‑GTGGTCTTGCTCAATGAGGTTATTCAGGTATTTTGTTGTACAATGGCAGTCAgg                                                                                                                                    >  2:508064/1‑141 (MQ=14)
          agCGTTAATACACTCAATACCTTTTACAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACG‑GTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgttt                                                                                                                                 <  1:552536/141‑1 (MQ=255)
               aaaTACACTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACG‑GTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagtt                                                                                                                            <  2:748206/141‑1 (MQ=255)
                aaTACATTCAATACCTTTTAAAGTAT‑TCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACG‑GTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagttt                                                                                                                           <  1:710814/141‑1 (MQ=255)
                aaTACACTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATTCCTTGTCTTTCTTACTTTAATATGACG‑GTGATCTTGCTCAATGGGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagttt                                                                                                                           <  1:657588/141‑1 (MQ=255)
                aaTACACTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACG‑GTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagttt                                                                                                                           >  1:305866/1‑141 (MQ=255)
                 aTACATTCAATACCTTTTAAAGGAT‑TCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACG‑GTGATCTTGCTCAATGAGGTTATTCAGATATTTTGATGTACAATGACAGTCAGgtttaagttta                                                                                                                          <  2:299340/141‑1 (MQ=255)
                  tACACTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACG‑GTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagtttaa                                                                                                                         >  2:238465/1‑141 (MQ=255)
                  tACACTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACG‑GTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgcttaagtttaa                                                                                                                         >  1:243425/1‑141 (MQ=255)
                  tACACTCAATACCTTTCACAGTTT‑TCTTTGCTGTATTGATACTTTGATAACTTGTCTTTCTTACTTTAATATGACG‑GTGATCTTGCTCTATTTGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagtttaa                                                                                                                         <  1:256176/141‑1 (MQ=14)
                    cactCAAAACCTTTTAAAGTAT‑TCGTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACG‑GTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCCGTCAGGTTTAAGTTTaaaa                                                                                                                       >  1:488436/4‑141 (MQ=14)
                        taaTGCCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACG‑GTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCtt                                                                                                                   <  2:187510/140‑1 (MQ=255)
                         aaTACCTTTTAAAGTAT‑TCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACG‑GTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCttt                                                                                                                  <  2:562040/141‑1 (MQ=255)
                          aTACCTTTTATAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACG‑GTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTa                                                                                                                 <  2:581271/141‑1 (MQ=255)
                           tACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACG‑GTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTaa                                                                                                                <  2:176775/141‑1 (MQ=255)
                            tccTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACG‑GTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAAt                                                                                                               <  1:646943/140‑1 (MQ=255)
                            aCCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACG‑GTGATCTTGCTCAATGAGGTTATTCAGATCTTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAAt                                                                                                               >  1:272117/1‑141 (MQ=255)
                            aCCTTTTAAAGTAT‑TCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACG‑GTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAAt                                                                                                               <  2:642082/141‑1 (MQ=255)
                             ccTTTTAAAGTAT‑TCTTTGGTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACG‑GTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAAtt                                                                                                              >  2:354292/1‑141 (MQ=255)
                               ttttAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAAAATGACG‑GTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTAc                                                                                                            >  1:145989/1‑141 (MQ=255)
                                  tAAAGTAT‑TCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACG‑GTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTAtttt                                                                                                         >  1:426055/1‑141 (MQ=255)
                                   aaaGTAT‑TCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACG‑GTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTACTTATTTTa                                                                                                        >  2:446406/1‑141 (MQ=255)
                                      gTAT‑TCTTTGCCGTATTGATACTTTGATCTCTTGTTTTTCTTACTTTAATATGACG‑GTGATCTTGCTCAATGGGGTTATTCAGATATTTCGATGTACATGTACACTCAGGTTTAAGTTTGAAGGTTTTAATTATTTTAGcc                                                                                                     <  2:87454/141‑1 (MQ=11)
                                        aT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACG‑GTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAGAAGCTTTAATTACTCTAGACAt                                                                                                   >  2:417626/1‑141 (MQ=255)
                                        aT‑TCTTTGCCGTATTGGTACTTTGATATCTTGTCTTTCTTACTTTAATATGACG‑GTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCAt                                                                                                   >  1:382984/1‑141 (MQ=255)
                                           tCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACG‑GTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCAttt                                                                                                 >  2:128045/1‑140 (MQ=255)
                                           tCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACG‑GTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTg                                                                                                 <  1:225062/141‑1 (MQ=255)
                                            cTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACG‑GTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGc                                                                                                <  1:264926/141‑1 (MQ=255)
                                             tttggcgTATTGATACTTTGATATCTTGTCTTTTTTACTTTAATATGACG‑GTGATCTTGCTCAATTAGGTTATTCAGATATTTCTATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTGATTATTTTAGCCATTGCt                                                                                               <  2:66904/135‑1 (MQ=14)
                                             tttGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACG‑GTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCt                                                                                               >  2:103160/1‑141 (MQ=255)
                                              ttGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACG‑GTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAGACTTTGGTTATTTTAGCCATTGCtt                                                                                              >  1:76804/1‑140 (MQ=14)
                                                gCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACT‑TTGATCTTGCTCAATGTGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTAcc                                                                                            >  1:598461/1‑141 (MQ=11)
                                                 ccgTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACG‑GTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCt                                                                                           <  2:592981/139‑1 (MQ=255)
                                                 cTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATTTGAGG‑TTTTTCTTGCTTAATGAGGTTATTCAGATATTTCGTTGTATAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCt                                                                                           <  1:210419/141‑1 (MQ=14)
                                                   gTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATAT‑ACGAGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTc                                                                                         >  1:338295/1‑141 (MQ=255)
                                                    tATTTATACTTTGATATCTTTTCTTTCTTACTTTAATATGACG‑GTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCg                                                                                        <  2:699844/141‑1 (MQ=14)
                                                    tATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACG‑GTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCg                                                                                        >  2:709688/1‑141 (MQ=255)
                                                     aTTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACG‑GTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGt                                                                                       <  1:452669/141‑1 (MQ=255)
                                                     aTTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACG‑GTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGt                                                                                       <  2:762752/141‑1 (MQ=255)
                                                       tGATACTTTGATACCTTGTCTTTCTTACTTTAATATGCCG‑GTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTACCCATTTCTACCTTCGTTg                                                                                     >  1:367117/1‑141 (MQ=255)
                                                         aTACTTTGATATCTTGTCTTTCTTACTTTAATATGACG‑GTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGaa                                                                                   <  1:438812/141‑1 (MQ=255)
                                                          tACTTTGATATCTTGTCTTTCTTACTTTAATATGACG‑GTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAg                                                                                  <  2:393663/141‑1 (MQ=255)
                                                           aCTTTGTTACCTTGTCTTTCTTACTTTAATATGACG‑GTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAACGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTAGCTTCGTTGAAgg                                                                                 <  2:285700/141‑1 (MQ=255)
                                                            cTTTGATATCTTGTCTTTCTTACTTTAATATGACG‑GTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGt                                                                                >  2:487766/1‑141 (MQ=255)
                                                              ttGATACCTTGTCTTTCTTACTTTAATATGACG‑GTGATGTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGc                                                                              >  1:493847/1‑141 (MQ=255)
                                                                 aTACCTTGTCTTTCTTACTTTAATATGACG‑GTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTg                                                                           >  1:510645/1‑141 (MQ=255)
                                                                  tACCTTGTCTTTCTTACTTTAATATGACG‑GTGATCTTGCTCAATGAGGTTCTTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGa                                                                          >  1:13876/1‑141 (MQ=255)
                                                                   aCCTTGTCTTTCTTACTTTAATATGACG‑GTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGAt                                                                         >  2:368461/1‑141 (MQ=255)
                                                                    ccTTGTCTTTCTTACTTTAATATGACG‑GTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATc                                                                        >  1:581133/1‑141 (MQ=255)
                                                                     cTTGTCTTTCTTACTTTAATATGACG‑GTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTTCTGCCTTCGTTGAAGGTGCCTGATCt                                                                       >  1:19706/1‑141 (MQ=255)
                                                                       tGTCTTTCTTTCTTTAATATGACG‑GTGATCTTGCTTAATGGGGTTATTCTGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGt                                                                     <  2:244059/141‑1 (MQ=11)
                                                                        gTCTTTGTTACTTGAATATGTCG‑GTGATCTTGATGAATGAGGTTATTCAGATATATCGTTGTACACTGACAGTCAGGGTTAGGTTTATAAGCTTTAATTCCTTTAGCCATTGCTACCTTCGTTGAAGGTCCCGGATCTGTa                                                                    >  2:261176/1‑141 (MQ=11)
                                                                         tctttcttACTTTAATATGACG‑GTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTaa                                                                   >  1:162924/1‑141 (MQ=255)
                                                                         tctttcttACTTTAATATGACG‑GTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTaa                                                                   >  1:293392/1‑141 (MQ=255)
                                                                             tcttACTTTAATATGACG‑GTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACTATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTAc                                                               >  1:566836/1‑141 (MQ=255)
                                                                             tcttACTTTAATATGACG‑GTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTAc                                                               <  2:148098/141‑1 (MQ=255)
                                                                              cttACTTTAATATGGCG‑GTGATCTTGCTCAATGAGGTTCTTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACAATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAAttcac                                                              >  2:220136/1‑138 (MQ=14)
                                                                              cttACTTTAATATGACG‑GTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTAcc                                                              <  1:329494/141‑1 (MQ=255)
                                                                               ttACTTTAATATGACG‑GTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGAACtaatatgttct                                                             >  2:156675/1‑131 (MQ=14)
                                                                               ttACTGTAAGAGGACG‑GTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAGAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCt                                                             <  2:634764/141‑1 (MQ=255)
                                                                                 tcTTTAATATGATG‑GTGATTTTGCTTAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTATCTACtttt                                                           <  2:326434/140‑1 (MQ=11)
                                                                                 aCTTTAATATGACG‑GTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCttt                                                           >  1:416487/1‑141 (MQ=255)
                                                                                  cTTTAATATGACG‑GTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTATAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCtttt                                                          >  2:571161/1‑141 (MQ=255)
                                                                                    ttAATATGACG‑GAGATCTTGCTCAATGAGGTTAGTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTATAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTTATCTGTAATTACCTTTTGa                                                        <  2:429928/141‑1 (MQ=11)
                                                                                     tAATATGACG‑GTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAg                                                       >  1:543504/1‑141 (MQ=25)
                                                                                           gACG‑GTGATCTTGCTCACGGTGGTTTTTCCGCTATTTCGATGTACAATTACAGTCAGGTTTAAGTTTAAAAGTTTTTATTATTTTAGCCATGGCTGCCGTCGTTGAAGATACCTGATCTGTAACTACCTTTTGATGCTTAc                                                 <  2:6891/141‑1 (MQ=2)
                                                                                            aCG‑GTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAcc                                                >  1:720070/1‑141 (MQ=21)
                                                                                                gTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGTTCTGTAACTACCTTTTGAGGCTTACCaaa                                             >  2:312583/1‑141 (MQ=11)
                                                                                                    tCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGt                                         >  1:464104/1‑141 (MQ=255)
                                                                                                     cTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGtt                                        >  2:656084/1‑141 (MQ=17)
                                                                                                     cTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGtt                                        <  1:120466/141‑1 (MQ=11)
                                                                                                      ttGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGttt                                       >  1:501132/1‑141 (MQ=255)
                                                                                                        gcgccaTGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTaa                                     <  2:140281/136‑1 (MQ=11)
                                                                                                        gCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTAGCTTTTGAGGTTTACCAAATTGTTTaa                                     >  2:202485/1‑141 (MQ=12)
                                                                                                        gCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTAACTTTTGAGGTTTACCAAATTGTTTaa                                     >  1:480405/1‑141 (MQ=12)
                                                                                                            aaTGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTATAATTACCTTTTGAGGTTTTCCAAATTGTTTAATgag                                 >  1:697316/1‑141 (MQ=255)
                                                                                                             aTGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGGTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTTGTTGAAGGTGCCTGATTTGTTATTACCTTTTGAGGTTTTCGAAATGTTTTAAtagga                                >  2:497477/1‑137 (MQ=11)
                                                                                                              tGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGAc                               >  2:4093/1‑141 (MQ=17)
                                                                                                              tGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGAc                               >  2:547343/1‑141 (MQ=12)
                                                                                                               gAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACg                              <  1:100419/141‑1 (MQ=255)
                                                                                                                aGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGt                             <  1:662342/141‑1 (MQ=12)
                                                                                                                aGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGt                             <  2:618529/141‑1 (MQ=17)
                                                                                                                 ggTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTGGAGGTTTACCAAATTGTTTACTGAGACGtt                            >  2:718771/1‑141 (MQ=255)
                                                                                                                  ggttgtcagacttgtCGATGTACAATGGTAGTCAGGTTTAAGTTTAAAGGCTTTTATTACTTTAACCATTGCTACCTTCGTTGAAGGGGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGttt                           <  2:362250/127‑1 (MQ=11)
                                                                                                                    tATTCAGTTATTTCGACGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGa                         <  2:119144/141‑1 (MQ=255)
                                                                                                                    tATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGCGGCTTACCAAATTGTTTAATGAGACGTTTGa                         >  2:736426/1‑141 (MQ=11)
                                                                                                                          gATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACg                   >  1:182905/1‑141 (MQ=255)
                                                                                                                           atatTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGc                  >  1:111524/1‑141 (MQ=12)
                                                                                                                            tatTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGCTAAACGCa                 >  2:529556/1‑141 (MQ=11)
                                                                                                                             atTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCat                <  2:478516/141‑1 (MQ=255)
                                                                                                                                tCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATg             >  1:621793/1‑141 (MQ=11)
                                                                                                                                 cGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGc            <  1:313861/141‑1 (MQ=255)
                                                                                                                                    tGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATCACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGCGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGa         >  1:350430/1‑141 (MQ=255)
                                                                                                                                      tACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGACGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAAt       >  1:678825/1‑141 (MQ=12)
                                                                                                                                        cAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGa     >  1:734633/1‑141 (MQ=14)
                                                                                                                                         aaTGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGAt    <  2:459698/141‑1 (MQ=11)
                                                                                                                                          aTGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGAtt   >  1:525952/1‑141 (MQ=255)
                                                                                                                                           tGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTa  >  2:268408/1‑141 (MQ=31)
                                                                                                                                             |                                                                                                                                          
TTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTAT‑TCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACG‑GTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTA  >  CP000730/35948‑36225

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑