Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 36,133 A→G 30.5% V140A (GTA→GCA)  USA300HOU_0026 ← IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073036,1330AG30.5% 568.6 / inf 592V140A (GTA→GCA) USA300HOU_0026IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base A (209/201);  new base G (83/98);  total (293/299)
Fisher's exact test for biased strand distribution p-value = 2.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.35e-01

Only 100 of 603 total aligned reads displayed.

TTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCA  >  CP000730/35993‑36273
                                                                                                                                            |                                                                                                                                            
  gCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACTTTGATCTTGCTCAATGTGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTAcc                                                                                                                                            >  1:598461/1‑141 (MQ=11)
  gCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTGTAAGGTTAAAAGCTTTCATTACTTTAACCATTGCTagc                                                                                                                                            >  2:711849/1‑139 (MQ=14)
   cTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTTAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCt                                                                                                                                           <  2:19359/141‑1 (MQ=255)
   cTGTATGGATACTTTGATACTTTCTCTTTCTTACTTTAATATGACGGTGATCTTTCTCAATGAGGTTATTCCGATATCTTGATGTACAATGGCCGGCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCt                                                                                                                                           <  1:130644/141‑1 (MQ=14)
    tGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCtt                                                                                                                                          <  2:271267/141‑1 (MQ=255)
     gTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGCTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGcttccttc                                                                                                                                         >  2:450344/1‑141 (MQ=255)
         tGATGCTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTg                                                                                                                                     <  2:642429/141‑1 (MQ=255)
         tGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTg                                                                                                                                     <  1:343707/141‑1 (MQ=255)
            tACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCTTTGCTGCCTTAGTTGAAg                                                                                                                                  >  1:81130/1‑141 (MQ=14)
            tACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAg                                                                                                                                  >  1:424340/1‑141 (MQ=255)
            tACTTTGACCTCTTGTTCTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTCCTACCTTCGTTGAAg                                                                                                                                  <  1:384145/141‑1 (MQ=14)
             aCTTTGTTACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAACGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTAGCTTCGTTGAAgg                                                                                                                                 <  2:285700/141‑1 (MQ=255)
             aCTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAgg                                                                                                                                 <  2:147829/141‑1 (MQ=255)
              ttttGATACTTTGTTTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTcaacgaaggt                                                                                                                                <  2:499232/140‑10 (MQ=11)
              cTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGt                                                                                                                                >  2:487766/1‑141 (MQ=255)
               tttGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTg                                                                                                                               <  1:661970/141‑1 (MQ=255)
               tttGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTg                                                                                                                               <  1:238573/141‑1 (MQ=255)
                ttGATATCTTGTCTTGTTTACTTTAATATGACGGTTATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGc                                                                                                                              <  1:73183/141‑1 (MQ=11)
                ttGATACCTTGTCTTTCTTACTTTAATATGACGGTGATGTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGc                                                                                                                              >  1:493847/1‑141 (MQ=255)
                ttGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGc                                                                                                                              >  1:442690/1‑141 (MQ=255)
                 tGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTAAGATATTTCGATGTACAATGTCAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGcc                                                                                                                             <  1:221218/141‑1 (MQ=11)
                 tGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGcc                                                                                                                             <  2:74125/141‑1 (MQ=255)
                 tGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGcc                                                                                                                             <  1:547912/141‑1 (MQ=255)
                  gATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCt                                                                                                                            >  1:30781/1‑141 (MQ=255)
                   atatcTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTg                                                                                                                           <  1:360370/137‑1 (MQ=255)
                    tatcTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGa                                                                                                                          >  2:581416/4‑141 (MQ=255)
                     aCCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTCAAGTCTTAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGAt                                                                                                                         >  1:145133/1‑141 (MQ=14)
                      tcTTGTCTTTCTTACATTAATATGACCGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTGTAAGTTTAAAAGCATTAATTATTTTAGCCATTGCTGCATTCGTTGAAGGTGCCTGATc                                                                                                                        <  1:464991/140‑1 (MQ=11)
                      tcTTGTCTTGCTTACTTTAATATGACGGTGACCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATc                                                                                                                        <  2:574678/140‑1 (MQ=255)
                      ccTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATc                                                                                                                        >  1:581133/1‑141 (MQ=255)
                          gTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTATAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTa                                                                                                                    >  2:696541/1‑141 (MQ=38)
                          gTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTa                                                                                                                    <  1:654880/141‑1 (MQ=255)
                           tctttcttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTaa                                                                                                                   <  1:618072/141‑1 (MQ=255)
                              ttcttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGCTATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTaacta                                                                                                                >  1:108540/1‑138 (MQ=255)
                               tcttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTaa                                                                                                               >  1:504859/1‑140 (MQ=255)
                                cttACTTTAATATGGCGGTGATCTTGCTCAATGAGGTTCTTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACAATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAAttcac                                                                                                              >  2:220136/1‑138 (MQ=14)
                                cttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTAcc                                                                                                              >  2:32049/1‑141 (MQ=255)
                                 ttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCt                                                                                                             <  2:393674/141‑1 (MQ=255)
                                 ttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCt                                                                                                             >  2:356391/1‑141 (MQ=38)
                                 ttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCt                                                                                                             >  1:27754/1‑141 (MQ=38)
                                 ttACCTGAATTTGACGGGTATCTTGCTCAATGAGGTTACTCAGATATTTCGACGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATCACTTTAACCATTGCTACCTTCGTGGAAGGGGCCTGATCTGTAATTACCt                                                                                                             <  1:54312/141‑1 (MQ=11)
                                   aCTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCttt                                                                                                           >  1:416487/1‑141 (MQ=255)
                                      ttAATATGACGGTGATCGTGCTCAATGCGGTCATTCAGATATTCCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTACTTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGa                                                                                                        >  2:629207/1‑141 (MQ=11)
                                      ttAATATGACGGAGATCTTGCTCAATGAGGTTAGTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTATAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTTATCTGTAATTACCTTTTGa                                                                                                        <  2:429928/141‑1 (MQ=11)
                                         atatGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGt                                                                                                     <  1:115981/141‑1 (MQ=25)
                                          tatGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAggct                                                                                                    <  1:695676/141‑3 (MQ=255)
                                          aatGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAggct                                                                                                    <  2:126809/140‑3 (MQ=255)
                                            tGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTa                                                                                                  <  2:178370/141‑1 (MQ=21)
                                             gACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTGAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTAc                                                                                                 <  1:295541/141‑1 (MQ=11)
                                             gACGGTGATCTTGCGCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAc                                                                                                 <  1:326033/141‑1 (MQ=255)
                                              aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAATTTTAAGAGCTTTAATTACCTTAATCATTGCTATGTTCGTTGTAGGTGCCTGTTCTGTAATTACCTTTTGAGGTTTAcc                                                                                                >  2:349657/1‑141 (MQ=11)
                                              aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAcc                                                                                                >  1:335722/1‑141 (MQ=21)
                                              aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAcc                                                                                                >  1:720070/1‑141 (MQ=21)
                                              aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCGGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGCTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGATTAcc                                                                                                >  2:590852/1‑141 (MQ=11)
                                              aCGGTGATCTTACTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTCATTACCTTTTGAGGTTTTcc                                                                                                >  2:754082/1‑141 (MQ=11)
                                               cagttaTCTTGCTCAATGAGGTTATTGAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTTCGGTTTACCa                                                                                               <  1:562395/136‑1 (MQ=11)
                                                 gcgATCCTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCaaa                                                                                             <  2:553944/139‑1 (MQ=11)
                                                 gTGATCTTGCTCAATGAGGTTATTGAGAAATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCaaa                                                                                             >  1:487970/1‑141 (MQ=11)
                                                 gTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCaaa                                                                                             <  1:196750/141‑1 (MQ=17)
                                                 gTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCaaa                                                                                             <  2:238871/141‑1 (MQ=11)
                                                     tCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGGTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGGTTACCAAATTGt                                                                                         >  2:673602/1‑141 (MQ=255)
                                                       ttGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTTCCTGATCTGTAATTACCTTTTGAGCTGTACCAAATTGttt                                                                                       >  2:307642/1‑141 (MQ=12)
                                                         gcgccaTGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTaa                                                                                     <  2:140281/136‑1 (MQ=11)
                                                         gCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTAACTTTTGAGGTTTACCAAATTGTTTaa                                                                                     >  1:480405/1‑141 (MQ=12)
                                                            cAATGAGGTTATTCAGATTTTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGCCATTGATTTCTCATGTTTACCAAATTGTTTAATga                                                                                  <  1:184072/141‑1 (MQ=11)
                                                               tGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGAc                                                                               >  2:4093/1‑141 (MQ=17)
                                                                gAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACg                                                                              >  2:725887/1‑141 (MQ=12)
                                                                gAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACg                                                                              <  1:100419/141‑1 (MQ=255)
                                                                 aGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGt                                                                             <  2:363073/141‑1 (MQ=17)
                                                                 aGGTTATTCAGATATTTCCATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAAGGAGACGt                                                                             <  2:243589/141‑1 (MQ=255)
                                                                  ggTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGtt                                                                            >  2:659866/1‑141 (MQ=32)
                                                                    ttATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTg                                                                          <  2:10550/141‑1 (MQ=11)
                                                                     tATTCAGTTATTTCGACGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGa                                                                         <  2:119144/141‑1 (MQ=255)
                                                                     tATTCAGATATTTCGCAGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGa                                                                         >  2:545377/1‑141 (MQ=11)
                                                                      aTTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGAt                                                                        >  2:518947/1‑141 (MQ=11)
                                                                          aGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAAc                                                                    <  1:622464/141‑1 (MQ=17)
                                                                          aGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAAc                                                                    <  1:374693/141‑1 (MQ=255)
                                                                           tatatTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACg                                                                   <  2:76460/140‑1 (MQ=12)
                                                                             tatTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCa                                                                 <  1:708921/141‑1 (MQ=11)
                                                                             tatTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCa                                                                 <  2:613243/141‑1 (MQ=11)
                                                                              atTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCat                                                                <  2:478516/141‑1 (MQ=255)
                                                                              atTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCATGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCat                                                                <  2:566836/141‑1 (MQ=11)
                                                                                ttCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCatat                                                              <  1:251451/141‑1 (MQ=17)
                                                                                   gATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAACAATTGCTGCCTTCGTTGAAGTTGCCTGATCTGTAACTAACTTTTTAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCt                                                           >  1:453784/1‑141 (MQ=11)
                                                                                   gATGTACAATGACAGTCAGGTTTAAGGTTAAAAGCTTTAATTATTTTAGACATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCAAATgat                                                           >  2:155933/1‑139 (MQ=11)
                                                                                          aaTGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGAt                                                    <  2:459698/141‑1 (MQ=11)
                                                                                           aTGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGAtt                                                   >  1:525952/1‑141 (MQ=255)
                                                                                           aTGACAGTCAGGATTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTACGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGAtt                                                   >  2:301171/1‑141 (MQ=11)
                                                                                            tGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTa                                                  <  2:256046/141‑1 (MQ=255)
                                                                                                                aacaGCTTCAATTACTTTAACCATTGCTACCTTGGTTGAAGGTGCCTGATCTGTCATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCa                              <  1:265985/138‑1 (MQ=1)
                                                                                                                               tttAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTTTCTCGTTGCTTACGCAACCAAATATCTAatgtatg               >  1:209807/1‑141 (MQ=11)
                                                                                                                                ttAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAatgtatgt              <  2:210315/141‑1 (MQ=14)
                                                                                                                                 taaccATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTc             <  2:416487/138‑1 (MQ=17)
                                                                                                                                 tAGCCATAGCTGCCTTCGTTGAAGGTGCCTGATCTGTAAATACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAATCAAACATCTAATGTATGTc             <  1:500566/141‑1 (MQ=11)
                                                                                                                                     cATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCctc         <  2:8357/141‑1 (MQ=12)
                                                                                                                                     cATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCctc         <  1:676248/141‑1 (MQ=32)
                                                                                                                                         gCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCa     <  2:686148/141‑1 (MQ=32)
                                                                                                                                           tACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATc   <  2:329205/141‑1 (MQ=32)
                                                                                                                                           tACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATc   >  1:161876/1‑141 (MQ=32)
                                                                                                                                           tACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCGAATGTATGTCCCTCTGCagc   <  2:376419/141‑3 (MQ=17)
                                                                                                                                            |                                                                                                                                            
TTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCA  >  CP000730/35993‑36273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: