BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,161	T→C	29.0%	I131V (ATT→GTT)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,161	0	T	C	29.0%	571.1 / inf	554	I131V (ATT→GTT)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base T (208/182); new base C (75/85); total (285/269)
Fisher's exact test for biased strand distribution p-value = 1.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 565 total aligned reads displayed.

CTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTT‑AAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTC  >  CP000730/36021‑36301

   tcttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTT‑AAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTaa                                                                                                                                           >  1:504859/1‑140 (MQ=255)

   tcttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTT‑AAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTAc                                                                                                                                           <  2:281380/141‑1 (MQ=255)

   tcttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTT‑AAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTAc                                                                                                                                           <  2:148098/141‑1 (MQ=255)

     ttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTT‑AAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCt                                                                                                                                         <  1:298843/141‑1 (MQ=255)

     ttACTGTAAGAGGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTT‑AAGAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCt                                                                                                                                         <  2:634764/141‑1 (MQ=255)

       aCTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTT‑AAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCttt                                                                                                                                       >  1:416487/1‑141 (MQ=255)

        cTTTAATATGACGGAGATCTTGCTCAATGAGGTTATTCAGATATTTCGTTGTACAATGACCGTCAGGTTTAAGTTT‑AAAAGCTATACTTGTTTTAGCAATTCCTGCCTTCGTTGAAGGTGCCTGATCTGTGACTACCtttt                                                                                                                                      >  2:313911/1‑141 (MQ=11)

          ttAATATGACGGAGATCTTGCTCAATGAGGTTAGTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTT‑AAAAGCTATAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTTATCTGTAATTACCTTTTGa                                                                                                                                    <  2:429928/141‑1 (MQ=11)

           tAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTT‑AAAAGCTTTAATTACTTTAACCATTGCTACCGTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTAAg                                                                                                                                   >  1:711759/1‑141 (MQ=255)

             atagGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTT‑AAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGt                                                                                                                                 <  2:186360/141‑1 (MQ=255)

              tatGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTT‑AAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAggct                                                                                                                                <  1:695676/141‑3 (MQ=255)

                 gACGGTGATCTTGCGCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTT‑AAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAc                                                                                                                             <  1:326033/141‑1 (MQ=255)

                  aCGGTGGTCTTGCTCAATGAGGTTATTCAGATAGTTCGATGTACAATGACAGTCAGGTTTAAGTTT‑AAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTAcc                                                                                                                            <  2:139287/141‑1 (MQ=11)

                  aCGGTGATCTTGCTCAATGAGGTTATTCAGGTATTTCGATGTACAATGGCAGTCAGGTTTAAGTTT‑AAAAGCTTTAATTACTTTAACCAGTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAcc                                                                                                                            <  2:56711/141‑1 (MQ=255)

                  aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTT‑AAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAcc                                                                                                                            <  1:737126/141‑1 (MQ=255)

                  aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTT‑AAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAcc                                                                                                                            >  1:335722/1‑141 (MQ=21)

                  aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTT‑AAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGACGTTTatc                                                                                                                            <  2:470519/141‑3 (MQ=255)

                   cGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTT‑AAAAGCTGTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTTTATCTACCTTTTGAGGATTACCa                                                                                                                           >  2:619969/1‑141 (MQ=11)

                     gTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTT‑AAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCaaa                                                                                                                         <  1:196750/141‑1 (MQ=17)

                         tCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTACGTTT‑AAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGt                                                                                                                     >  1:677423/1‑141 (MQ=12)

                         tCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTT‑AAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGt                                                                                                                     >  2:662342/1‑141 (MQ=12)

                         tCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTT‑AAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGt                                                                                                                     >  2:325424/1‑141 (MQ=12)

                         tCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTGAAGTTT‑AAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGt                                                                                                                     >  2:125594/1‑141 (MQ=12)

                          tttGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTT‑AAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGtt                                                                                                                    <  2:736368/140‑1 (MQ=12)

                          cTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTT‑AAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGtt                                                                                                                    <  2:48010/141‑1 (MQ=17)

                           ttGCTCAATGAGGTTATTCAGATAGTTCGATGTACAATGGCAGTCAGGTTTAAGTTT‑AAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATGACCTTTTGAGGTTTACCAAATTGttt                                                                                                                   >  1:487215/1‑141 (MQ=255)

                                ccaTGAGGTTATTCATATATTTCGATGTACATGGGCATTCAGGTTTAAGTGT‑AAAAGCTTTAATTACTTTAACCATTGATACCTTCCTTGAATTGGCCTTATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATga                                                                                                              <  2:695564/139‑1 (MQ=11)

                                cAATGAGGTTATTCAGATTTTTCGATGTACAATGGCAGTCAGGTTTAAGTTT‑AAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGCCATTGATTTCTCATGTTTACCAAATTGTTTAATga                                                                                                              <  1:184072/141‑1 (MQ=11)

                                cAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTT‑AAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATga                                                                                                              <  2:351935/141‑1 (MQ=12)

                                 aaTGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTT‑AAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATgag                                                                                                             >  1:544892/1‑141 (MQ=17)

                                   tGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTT‑AAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTAGAGGTTTACCAAATTGTTTAATGAGAc                                                                                                           >  2:296110/1‑141 (MQ=12)

                                    gAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTT‑AAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACg                                                                                                          <  1:100419/141‑1 (MQ=255)

                                     aGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTT‑AAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGt                                                                                                         >  1:665066/1‑141 (MQ=17)

                                      ggTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTT‑AAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGtt                                                                                                        >  2:58691/1‑141 (MQ=12)

                                      ggTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTT‑AAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGtt                                                                                                        >  1:36956/1‑141 (MQ=17)

                                        ttattaAGCTATTACGATGTACCATGGCAGTCAGGTTTAAGTTT‑AAAAGCTTTAATCACCTTAACCAGTGCTACCGTCGTTTAAGGCGCCTGATCTGTAGTTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTg                                                                                                      >  2:241539/1‑141 (MQ=11)

                                         tATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTT‑AAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGa                                                                                                     <  1:285269/141‑1 (MQ=17)

                                         tATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTT‑AAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGa                                                                                                     <  2:338051/141‑1 (MQ=17)

                                         tATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTT‑AAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGa                                                                                                     <  1:428345/141‑1 (MQ=11)

                                          aTTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTT‑AAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGAt                                                                                                    >  1:271496/1‑141 (MQ=17)

                                           ttCAGATATTCCTATGCACAATGGCAGTCAGGTTTACGTTT‑AAAAGCTTTACTTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTTATTACCTTTTGACGTTTACCAAATTGTTTAATGAGACGTTTGATa                                                                                                   <  1:203056/141‑1 (MQ=11)

                                               gATATTTCGATTTACAATGGCAGTCAGGTTTAAGTTT‑AAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACg                                                                                               <  2:408400/141‑1 (MQ=12)

                                               gATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAAGCTTTGATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAAc                                                                                                <  2:170029/141‑1 (MQ=12)

                                                  ttttCGATGTACAATGACAGTCAGGTTTAAGTTT‑AAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCat                                                                                            <  2:207482/140‑1 (MQ=17)

                                                  atTTCGATGTACAATGGCAGTTAGGTTTAAGTTT‑AAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCat                                                                                            <  1:108185/141‑1 (MQ=12)

                                                    ttCGATGTACAATGGCAGTCAGGTTTAAGTTT‑AAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCatat                                                                                          <  1:251451/141‑1 (MQ=17)

                                                     tCGATGTACAATGACAGTCAGGTTTAAGTTT‑AAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATg                                                                                         >  1:621793/1‑141 (MQ=11)

                                                      cGATGTACAATGGCAGTCAGGTTTAAGTTT‑AAAAGCTTTAATTACTTGAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGc                                                                                        <  2:651117/141‑1 (MQ=12)

                                                      cGATGTACAATGACAGTCAGGTTTAAGTTT‑AAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGc                                                                                        <  1:462789/141‑1 (MQ=11)

                                                      cGAGGTACAATGTCAGTCAGGTTTAAGTTT‑AAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCTTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGc                                                                                        <  1:535716/141‑1 (MQ=11)

                                                         tGTACAATGGCAGTCAGGTTTAAGTTT‑AAAAGCTTTAATCACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGCGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGa                                                                                     >  1:350430/1‑141 (MQ=255)

                                                            aCAATGGCAGTCAGGTTTAAGTTT‑AAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATg                                                                                  >  2:68154/1‑141 (MQ=12)

                                                            aCAATGACAGTCAGGTTTAAGTTT‑AAAAGCTTTAATTATTTTAGTCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATg                                                                                  <  2:362764/141‑1 (MQ=11)

                                                             cAATGACAGTCAGGTTTAAGTTT‑AAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTCATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGctgactga                                                                                 >  1:220143/1‑141 (MQ=17)

                                                               aTGACAGTCAGGTTTAAGTTT‑AAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGAtt                                                                               >  1:490986/1‑141 (MQ=255)

                                                                tGACAGTCAGGTTTAAGTTT‑AAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTa                                                                              <  2:256046/141‑1 (MQ=255)

                                                                                                    tttAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAatgtatg                                           <  2:422166/141‑1 (MQ=2)

                                                                                                     ttaaccATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTGGTTGCTTACGCAACCAAATATCTAatgtatgt                                          >  1:55758/5‑141 (MQ=14)

                                                                                                     ttAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAatgtatgt                                          <  1:722230/141‑1 (MQ=9)

                                                                                                        accATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTccc                                       >  1:707438/2‑141 (MQ=17)

                                                                                                        accATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTccc                                       <  2:77726/140‑1 (MQ=17)

                                                                                                        accATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATGACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCTAATATCTAATGTATGTccc                                       <  1:499382/140‑1 (MQ=17)

                                                                                                         ccATTGCTACCTTCGTAGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCct                                      >  1:382363/1‑141 (MQ=17)

                                                                                                          cATTACTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGTGGCTTACCAAATTGTTTAATTAGACGTTTGATAAACGCATATGCTGAATTATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCctc                                     <  1:606823/141‑1 (MQ=11)

                                                                                                           aTTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATACACGCATATGCTGAATGATTATCTCGTTGCTGACGCAACCAAATATCTAATGTATGTCCctct                                    <  1:115156/141‑1 (MQ=17)

                                                                                                            ttGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCCCTg                                   >  2:612451/1‑141 (MQ=17)

                                                                                                              gCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTgta                                 >  2:754291/1‑139 (MQ=17)

                                                                                                              gCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCa                                 <  2:550380/141‑1 (MQ=32)

                                                                                                                tACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATc                               >  1:161876/1‑141 (MQ=32)

                                                                                                                tACCTTCGGTGAAGGTGTCTTATCTGTAATTACCTTTTGATGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATc                               <  2:220143/141‑1 (MQ=255)

                                                                                                                 aCCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCCTCa                              >  1:124380/1‑141 (MQ=17)

                                                                                                                 aCCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCa                              <  1:562842/141‑1 (MQ=32)

                                                                                                                 aCCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCa                              >  2:306414/1‑141 (MQ=32)

                                                                                                                  ccttcttggcagcggCCTGATCTGGAATTACCTTTTGAGTTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCaa                             <  2:714478/127‑1 (MQ=11)

                                                                                                                  ccTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCaa                             <  2:490130/141‑1 (MQ=32)

                                                                                                                  ccTTCGTTGAAGGTGCCTGACCTATATACACCTTTTGAGGTTTACCAAATTATTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCCCTGCATcca                             >  2:544342/1‑139 (MQ=11)

                                                                                                                   cTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTTATGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGGATGTCCCTCTGCATCAAt                            >  1:39236/1‑141 (MQ=12)

                                                                                                                   cTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAAt                            >  2:117568/1‑141 (MQ=32)

                                                                                                                   cTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATATAATGTATGTTTCTCTGCATCaag                            <  2:449469/141‑2 (MQ=12)

                                                                                                                       aacgAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGGTTGATAAACGCATATGCTGAATGATTATCTCATTACTTACCCAACCAAATAGCTGCTGTAGGTCCCTCTCCATCAATGGcc                        >  2:573381/4‑140 (MQ=11)

                                                                                                                            aGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGatt                   >  1:145924/1‑140 (MQ=17)

                                                                                                                             ggTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGatat                  >  1:651477/1‑141 (MQ=17)

                                                                                                                             ggTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAAGGCATATGCTGAATGATTATCTGGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTTTATAAATGTGACGatat                  >  1:312650/1‑141 (MQ=11)

                                                                                                                             ggTGCCTGAGCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGatat                  <  2:109063/141‑1 (MQ=17)

                                                                                                                                 ccTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTTGTTGTCTATTCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAAt              >  1:594307/1‑141 (MQ=11)

                                                                                                                                 ccTGATCTGTAAGTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAAt              <  1:472534/141‑1 (MQ=17)

                                                                                                                                  cTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATa             <  2:235813/141‑1 (MQ=32)

                                                                                                                                    gATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGc           >  1:85895/1‑141 (MQ=17)

                                                                                                                                    gATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTAGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGc           >  1:518259/1‑141 (MQ=17)

                                                                                                                                    gATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTGTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGc           >  2:501490/1‑141 (MQ=17)

                                                                                                                                     aTCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAATTTGCt          >  1:584272/1‑141 (MQ=17)

                                                                                                                                       cTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATTTATGTCCCTCTTCATCAATGGCACGATATAAATAACTcc        >  2:45847/1‑141 (MQ=12)

                                                                                                                                       cTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTcc        >  1:236897/1‑141 (MQ=32)

                                                                                                                                       cTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTcc        <  1:450344/141‑1 (MQ=32)

                                                                                                                                        tGTATTTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCa       <  2:156297/141‑1 (MQ=17)

                                                                                                                                        tGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGCTATCAATAGCTCCa       <  2:520139/141‑1 (MQ=17)

                                                                                                                                        tGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCa       <  2:383297/141‑1 (MQ=12)

                                                                                                                                          tAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTACAtt     >  1:140698/1‑141 (MQ=17)

                                                                                                                                            aTTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCa       <  2:392234/137‑1 (MQ=33)

                                                                                                                                            aTTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCCAATGTATGCCTCTCTGCATCAATGGCACGATACAAATAGCTCCAattc   >  2:8606/1‑140 (MQ=11)

CTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTT‑AAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTC  >  CP000730/36021‑36301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`