BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,269	C→T	58.3%	A95T (GCA→ACA)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,269	0	C	T	58.3%	204.2 / inf	777	A95T (GCA→ACA)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base C (151/171); new base T (223/224); total (379/398)
Fisher's exact test for biased strand distribution p-value = 4.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

Only 100 of 782 total aligned reads displayed.

TGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGG  >  CP000730/36129‑36408

   tACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATc                                                                                                                                          >  1:467878/1‑141 (MQ=17)

   tACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATc                                                                                                                                          >  1:161876/1‑141 (MQ=32)

    aCCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCa                                                                                                                                         <  1:562842/141‑1 (MQ=32)

     ccTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCaa                                                                                                                                        <  2:235008/141‑1 (MQ=32)

                ggTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGataa                                                                                                                             >  1:607230/1‑140 (MQ=17)

                ggTGCCTGAGCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGatat                                                                                                                             <  2:109063/141‑1 (MQ=17)

                        aTCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATTGGCGGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAGTGGCACGATATAAATAGcc                                                                                                                     >  1:523920/1‑140 (MQ=11)

                          cTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATCGCTcc                                                                                                                   >  2:304974/1‑141 (MQ=17)

                           tGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCa                                                                                                                  <  2:383297/141‑1 (MQ=12)

                            ttatttACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCAt                                                                                                                 <  2:569441/137‑1 (MQ=12)

                                ttACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGCCGGGCGCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTc                                                                                                             <  1:751543/141‑1 (MQ=11)

                                 tACCTTTTGAGGCTTACCAAATTGTTTACTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTcc                                                                                                            <  1:533557/141‑1 (MQ=12)

                                  aCCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCt                                                                                                           <  2:665066/141‑1 (MQ=32)

                                    cTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCttt                                                                                                         <  1:145892/141‑1 (MQ=17)

                                    cTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCttt                                                                                                         <  2:651477/141‑1 (MQ=17)

                                    cTTTTGACGCTTACCAAATTTGTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTATTGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCttt                                                                                                         <  2:81752/141‑1 (MQ=11)

                                     ttttGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCtttt                                                                                                        >  1:710516/1‑141 (MQ=32)

                                         gAGCTTTACCAAATTTTTTAATGAGACGTTTGATACACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTAttt                                                                                                    <  1:7757/141‑1 (MQ=12)

                                           gttttACCAAATTGTTTAATGATACGTTTTATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTg                                                                                                  <  2:444354/139‑1 (MQ=255)

                                           ggcttACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTg                                                                                                  >  1:420670/4‑141 (MQ=17)

                                           ggTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTg                                                                                                  >  1:89458/1‑141 (MQ=32)

                                             tttACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGATTGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATATCTCTATTTTCCTTTTATTTTGAt                                                                                                >  1:486723/1‑141 (MQ=12)

                                              ttACCAAATTGTTTAATGAGCCGTTTGATAAACGCATATGCTGAATGATTATCTAGTTTGGTACTCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGAta                                                                                               <  1:365997/141‑2 (MQ=11)

                                               tACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGAtat                                                                                              <  1:477670/141‑3 (MQ=17)

                                                aCCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTa                                                                                             <  2:489824/141‑1 (MQ=32)

                                                  cAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCCCGATATAAATAGCTCCCTCTTCCTTTTATTTTGAtatccg                                                                                           >  2:147294/1‑136 (MQ=11)

                                                  cAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCCCCATTTTCCTTTTATTTTGATCTACg                                                                                           >  2:169258/1‑141 (MQ=12)

                                                   aaaTTGTTTAATGTGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGt                                                                                          <  1:270438/141‑1 (MQ=17)

                                                     aTTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGAtatgagttt                                                                                        >  1:286360/1‑133 (MQ=12)

                                                     aTTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCATGATATAAATAACTCAATTTTCCTTTTCTTTTGTTTTACGtct                                                                                        >  2:498777/1‑141 (MQ=11)

                                                      ttGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAGTATCTTTTGTATGTCCTTTTGTATCAATGGCACGATATAAATAGTTGCATTTTTCTTTTATTTTGATATAGGtctt                                                                                       >  1:120050/1‑140 (MQ=11)

                                                      ttGGTTAATGAGAAGTTTGAAAAACGCATATGCTGCATGATTTTCTCTTCGCCGACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATTGCACGAGATAAATAGCTTCATTTTCCTTTTATTTTGATATACGtctc                                                                                       <  1:590852/141‑1 (MQ=11)

                                                       tttttAATGATTCGTTTGATAAACGCATATTCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCtca                                                                                      <  1:325424/139‑1 (MQ=11)

                                                        gTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATTTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTTtcat                                                                                     >  1:320260/1‑141 (MQ=255)

                                                        gTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCtcat                                                                                     >  1:700041/1‑141 (MQ=32)

                                                           tAATGAGACGTTTGATGAACGGATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCaa                                                                                  >  1:395037/1‑141 (MQ=255)

                                                            aaTGAGGCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGCTGTACGTGTCATCAAt                                                                                 <  2:547745/141‑1 (MQ=12)

                                                            aaTGAGACGTTTGATAAACTCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATCGCACGTTATAAACAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAAt                                                                                 <  2:697385/141‑1 (MQ=11)

                                                            aaTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGCTATACGTCTCATCAAt                                                                                 <  2:372591/141‑1 (MQ=12)

                                                            aaTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAAt                                                                                 >  1:514914/1‑141 (MQ=17)

                                                            aaTGAGACGTTTGATAAACACATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGTTATACGTGtcttcatt                                                                                 >  1:499657/1‑139 (MQ=11)

                                                             aTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTATCATCATTTGGATATACGTCTCATCAATa                                                                                <  2:353350/141‑1 (MQ=11)

                                                              tGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATAc                                                                               <  1:216086/141‑1 (MQ=32)

                                                                agaCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGc                                                                             <  1:377237/141‑1 (MQ=17)

                                                                  aCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCa                                                                           >  2:248276/1‑141 (MQ=17)

                                                                  aCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCa                                                                           >  1:638222/1‑141 (MQ=17)

                                                                  aCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCa                                                                           >  2:391689/1‑141 (MQ=32)

                                                                   cGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTGGATGTAGTTCTCATCAATACGCCAt                                                                          >  1:437515/1‑141 (MQ=12)

                                                                     tttGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGTTTTACGTCTCATCAATACCCCAttt                                                                        >  2:205073/1‑141 (MQ=255)

                                                                      ttGATAAACGCATATGCTGAATGAGTAGCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTg                                                                       >  2:757977/1‑141 (MQ=255)

                                                                        taTAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGta                                                                     <  1:250904/140‑1 (MQ=17)

                                                                         aTAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGtaa                                                                    <  1:691555/141‑1 (MQ=32)

                                                                             aCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATATAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCACTTTGTAATAAg                                                                >  2:13135/1‑141 (MQ=255)

                                                                             aCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATATAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGATCAATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAg                                                                >  2:185458/1‑141 (MQ=255)

                                                                              cGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTACTAAGc                                                               >  2:412226/1‑141 (MQ=255)

                                                                               gCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATATATGTCCCTCTGCATCAATGGCACGATATAAATCGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCATTTCGCACTTTGTAATAAgat                                                              >  2:139679/1‑139 (MQ=11)

                                                                                   atGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCttttt                                                          <  1:659866/141‑1 (MQ=35)

                                                                                        ttatcaTTATCTCCTTACTCACGCAACCAAATATATTATGTATGTCCCTCCGTATCAATGGCACGATATAAATAGCTCCATTTGCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGc                                                     <  2:516655/136‑1 (MQ=14)

                                                                                        gAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGc                                                     <  1:353730/141‑1 (MQ=39)

                                                                                        gAATCATTAGCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGc                                                     <  1:240331/141‑1 (MQ=255)

                                                                                         aaTGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCt                                                    <  1:309875/141‑1 (MQ=40)

                                                                                          aTGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCtt                                                   >  2:428490/1‑141 (MQ=255)

                                                                                            gATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATATGTCTCATCGATACGCCAGTTCTAATAAGCtttttttttatttt                                                 >  1:299144/1‑133 (MQ=11)

                                                                                             aTTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCttttt                                                <  1:186332/141‑1 (MQ=255)

                                                                                              ttATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTTATGTACGTCTTATCAATCCGCCATTTGTGATAAGCTTTTTTATGAtttttt                                               >  1:341040/1‑140 (MQ=14)

                                                                                              ttATCTCGTTGCTTACGCAACCAAATATATAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATAAGCCCTTTGTCATAAGATTTTTTTTGTtatttt                                               >  2:680456/1‑140 (MQ=11)

                                                                                               tATCTCGTTGCTTACGCATCCAAATATCTTATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttct                                              <  1:9894/141‑1 (MQ=255)

                                                                                               tATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATTGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCTATCCGCCATTTTTACTCTGCATTTTTATTGttttttt                                              >  1:2305/1‑139 (MQ=11)

                                                                                                aTCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttctt                                             <  1:103160/141‑1 (MQ=255)

                                                                                                aTCTCGTAGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTCTAATACGCTTTTTTTTGCTTTttctt                                             >  2:321224/1‑141 (MQ=14)

                                                                                                 tctcTTTGCTTACGCATCCAAATATCTAATTTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttcttc                                            <  2:632253/141‑1 (MQ=255)

                                                                                                  ctcGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGCTATATATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATTGTTTTTCTTcc                                           >  1:677377/1‑141 (MQ=14)

                                                                                                  ctcGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATATGTCTCATCAATACGCTATTTGTATTAAGTTTTTTTATTCTTTtttttct                                           >  2:61904/1‑140 (MQ=14)

                                                                                                    cGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACATCTCATCAATCAGCCATTTGTAATAAGCATTTTTATGCTTATTCTGCCat                                         >  1:445818/1‑140 (MQ=11)

                                                                                                      ttGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCATTTTATCTTGTAGAAAGTCGCATCAAGAAGACAATGGTTATAAGCTTTTTTCTGCTCATAATTCCAAAt                                       <  2:734633/141‑1 (MQ=2)

                                                                                                        gCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAAttt                                     >  2:712199/1‑141 (MQ=40)

                                                                                                         cTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAACTAGCGCCATTTTCCTTTTATTTTGATATACGTCTCATAAATACGCCCTTTGTAATAAGCGTTTTTATGCTTTTTCTTCCCAAtttt                                    >  1:717457/1‑140 (MQ=11)

                                                                                                          ttACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGa                                   <  2:510533/141‑1 (MQ=40)

                                                                                                           tACGCAACCAAATATATAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGAt                                  >  2:277437/1‑141 (MQ=255)

                                                                                                            aCGCAACCAAATATCTAATGTATGTCCCCCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATa                                 <  2:266179/141‑1 (MQ=255)

                                                                                                             cGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATat                                <  2:533228/141‑2 (MQ=255)

                                                                                                             cGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATat                                >  2:474922/1‑140 (MQ=255)

                                                                                                             cGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATATCTCCATTTTCCTTTTATTTTGATGTACGTCTCATAAATACGCCATTTGTAATCAGCTTTTTTATGCTTTTTTTTCCAAATTTGAtgc                                >  1:134192/1‑139 (MQ=255)

                                                                                                             cGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATAc                                <  1:409666/141‑1 (MQ=40)

                                                                                                              gCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATata                               <  1:619969/141‑3 (MQ=255)

                                                                                                               cAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATTCTTTTTCTTCCAAATTTTATATaa                              >  2:2164/1‑141 (MQ=11)

                                                                                                                  ccAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATATGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAAt                           >  1:11288/1‑141 (MQ=255)

                                                                                                                        atCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGc                     >  2:335667/1‑141 (MQ=255)

                                                                                                                        atCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGc                     >  2:587301/1‑141 (MQ=255)

                                                                                                                           tAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCata                  <  2:288630/141‑1 (MQ=37)

                                                                                                                            aaTGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATTTACGTCTCCTCAATACGCCATTTTTAATAAGCTTTTTTATACTTTTTCTTCCATATTTGATATAAAATTGGGACctat                 >  1:175321/1‑141 (MQ=11)

                                                                                                                               gtatgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCt              <  1:733712/141‑1 (MQ=255)

                                                                                                                                tatgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCATTTTTTTTTGATGTACGTCTCATCATTATGACATTTGTAATATGATTTTTTATGCTTTTTATTGCACATTTGATATAAAATTAGGGattattatt             >  1:543934/1‑141 (MQ=11)

                                                                                                                                 atgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTg            <  2:725922/141‑1 (MQ=255)

                                                                                                                                 atgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTTTCTTCAATACTCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATGTTAAATTGGGGACTATTCttt            >  1:583127/1‑140 (MQ=11)

                                                                                                                                  tgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATAAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCATATTTGATATAAAATTtgggaaccgtattgc           >  2:600640/1‑130 (MQ=11)

                                                                                                                                    tCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAAc         <  1:388953/141‑1 (MQ=18)

                                                                                                                                    tCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATAAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCACATTCTTGAAc         >  2:455341/1‑141 (MQ=255)

                                                                                                                                       ctctGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCa      <  1:202485/141‑1 (MQ=21)

                                                                                                                                        cctGCATCACTGGCACGATATAAATAGCTCCCTTTTCCTTTTATTTTGCTGTACGTCTCATCAATACGCCATTTGTAATAATCTTTTTTATGCTTTTTCTTCAAAATTTGATATAAAATTGGGGCATATTCTTGAACCCaa     <  2:117472/140‑1 (MQ=11)

TGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGG  >  CP000730/36129‑36408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`