BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,315	G→A	59.1%	Y79Y (TAC→TAT)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,315	0	G	A	59.1%	260.2 / inf	794	Y79Y (TAC→TAT)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base G (138/177); new base A (220/239); total (372/422)
Fisher's exact test for biased strand distribution p-value = 2.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.08e-01

Only 100 of 800 total aligned reads displayed.

TTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTC  >  CP000730/36175‑36455

 ttccAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGt                                                                                                                                             <  1:439720/139‑1 (MQ=17)

  tccAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTa                                                                                                                                            <  2:711440/140‑1 (MQ=17)

   ccAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAGTGATGATGTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTAc                                                                                                                                           >  2:698030/1‑141 (MQ=12)

   ccAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGAGTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCACTCTGTATCAATGGCACGATATAACTAGATACACATTACTTTTATTTTGATTTAc                                                                                                                                           >  2:720421/1‑141 (MQ=11)

      atttGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGtc                                                                                                                                        <  1:246468/139‑1 (MQ=12)

      aaTTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATTAATGGCACGATTTAAATAGCTGCAGTTTCCTTGTATTTTGCTATACggc                                                                                                                                        >  1:70289/1‑139 (MQ=11)

       aTTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGGTTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATTTCCGtcc                                                                                                                                       >  2:498711/1‑140 (MQ=12)

       aTTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGtct                                                                                                                                       >  1:760195/1‑141 (MQ=17)

       aTTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGtcc                                                                                                                                       >  2:136002/1‑140 (MQ=17)

          gTTTAATGAGACGTTGGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCtcat                                                                                                                                    <  1:41386/141‑1 (MQ=12)

             tAATGAGACGTTTGATAAACGTATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCaa                                                                                                                                 <  1:261176/141‑1 (MQ=17)

             tAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTCTTTTGATATTAGTCTCTTCaa                                                                                                                                 >  1:91709/1‑141 (MQ=11)

              aaTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAAt                                                                                                                                >  1:514914/1‑141 (MQ=17)

              aaTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCtcatcact                                                                                                                                >  2:753447/1‑139 (MQ=17)

              aaTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCGCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAAt                                                                                                                                <  1:163072/141‑1 (MQ=17)

                tGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATAc                                                                                                                              <  1:238465/141‑1 (MQ=12)

                tGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATAc                                                                                                                              <  2:671427/141‑1 (MQ=32)

                      gTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCAtt                                                                                                                        <  1:209282/141‑1 (MQ=17)

                        ttGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTAGTTTGATATACGTCTCATCATTACGACATTTg                                                                                                                      >  2:210747/1‑141 (MQ=11)

                             aaaCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGtaata                                                                                                                 <  1:507358/141‑1 (MQ=17)

                               aCGCATATGCTGAATGATTATCTCGTTGCTTAAGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAg                                                                                                               <  1:293731/141‑1 (MQ=255)

                                cGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCCTCAATACGCCATTTGTAATAAGc                                                                                                              >  1:273827/1‑141 (MQ=25)

                                cGCATATGCTGAATGATTATCTCGTTGCTTACACAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGc                                                                                                              >  2:84743/1‑141 (MQ=255)

                                  cATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCCCCTCAATACGCCCTTTGTGATAAGCtt                                                                                                            >  2:461155/1‑141 (MQ=11)

                                  cATATGCTGAATGATTATCTCGTTGCTTACGCAAACAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGcgt                                                                                                            >  2:389113/1‑139 (MQ=255)

                                       gCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTTCTATACGTCTCATCAATACGCCATTTGTAATACGCttttttg                                                                                                       >  1:406504/1‑140 (MQ=11)

                                        cTGAATGATTGTCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATACTTCAACGGCCCGTTTATTTTGATATACGTCTCATCATTCCGCCCTTTGTAATAAGCTTTTTTAt                                                                                                      >  1:556859/1‑141 (MQ=11)

                                        cTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTAt                                                                                                      <  2:26486/141‑1 (MQ=37)

                                         tGAATGATTATCTCGTTGCTTACGCAACCAAATCTCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATg                                                                                                     <  2:542390/141‑1 (MQ=255)

                                         tGAATGATTATCTCGTTGCTTACGCAACCAAATATCCAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATg                                                                                                     <  1:324371/141‑1 (MQ=255)

                                               aTTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACTTTTTATCATTACGCCATTTGTATTAAGCTTTTTTTTGCttttt                                                                                               >  1:33424/1‑141 (MQ=14)

                                               aTTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTTTGtttttt                                                                                               >  2:371983/1‑141 (MQ=255)

                                                 tATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttct                                                                                             <  1:656084/141‑1 (MQ=255)

                                                 tATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttct                                                                                             >  2:658585/1‑141 (MQ=39)

                                                 tATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttct                                                                                             >  1:484715/1‑141 (MQ=40)

                                                   tctcGTTGCTTACGCTACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAATTATCTCCATTTTCCTTTTATTTTGATATACGTCTCGTCAATACTCCATTTGTAATCAGCTTTTTTATATTTTttctac                                                                                           >  1:221486/1‑139 (MQ=11)

                                                   tctcGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttcttc                                                                                           >  1:693375/1‑141 (MQ=255)

                                                   tctcGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttcttc                                                                                           <  2:684837/141‑1 (MQ=255)

                                                       gTTGCTTACGCAACCCAATATCTAATGTATGTCTCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTATTCCaaa                                                                                       >  1:570332/1‑141 (MQ=255)

                                                        ttGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCATTTTATCTTGTAGAAAGTCGCATCAAGAAGACAATGGTTATAAGCTTTTTTCTGCTCATAATTCCAAAt                                                                                      <  2:734633/141‑1 (MQ=2)

                                                         tGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAATTAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCGATTTGTAATAAGCTTTTTTTGTCTTTTTCTTCAAGAtt                                                                                     >  1:180305/1‑141 (MQ=11)

                                                          gCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATTCGCCATTTCTAATAGACTGTTTTATGCTTTTTCTTCCATAttt                                                                                    >  1:197290/1‑141 (MQ=11)

                                                           cTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATTTGAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTg                                                                                   <  1:716448/141‑1 (MQ=255)

                                                            ttACGAAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGa                                                                                  <  1:350704/141‑1 (MQ=255)

                                                              aCGCAACCAAATATCTAATGTATGTCTCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATa                                                                                <  1:429487/141‑1 (MQ=255)

                                                               cGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTACAAATTTGAtcc                                                                               >  1:134093/1‑139 (MQ=255)

                                                                gCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATata                                                                              <  1:619969/141‑3 (MQ=255)

                                                                gCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATata                                                                              <  2:425639/141‑3 (MQ=39)

                                                                gCAACCAAATATCTAATGTATGTACCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATata                                                                              <  1:58691/141‑3 (MQ=255)

                                                                 cAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATaa                                                                             >  2:201786/1‑141 (MQ=255)

                                                                    ccAAAAATCTAATGTATATCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATCTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAAt                                                                          <  2:488436/141‑1 (MQ=255)

                                                                        atatCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTAATTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTggg                                                                      >  2:566894/1‑141 (MQ=255)

                                                                        atatCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTggg                                                                      <  1:306414/141‑1 (MQ=37)

                                                                          atCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGc                                                                    >  2:335667/1‑141 (MQ=255)

                                                                            cTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCat                                                                  <  2:184415/141‑1 (MQ=255)

                                                                             tAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCata                                                                 <  2:288626/141‑1 (MQ=37)

                                                                              aaTGTATGTCCCTCTGTATCAATGGCACGATATAAATGGCTCCCTGCTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGCTATAAAATTGGGGCatat                                                                >  1:526982/1‑141 (MQ=11)

                                                                              aaTGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCatat                                                                >  1:371049/1‑141 (MQ=38)

                                                                               atgtatgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATTAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTGCAAATTTGATATAAAATTGGGGCATAtt                                                               >  1:745248/1‑141 (MQ=11)

                                                                               atgtatgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTCGATATAAAATTAGAGCTTAtt                                                               >  1:113210/1‑141 (MQ=11)

                                                                                tgtatgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTTATGTACGTCTCATCAATACGCCATTTGTAATAAGCGTTTTTATGTGTTTTCTTCCAAATTTGATATAAAATTGGTGCATATTc                                                              >  1:636178/1‑141 (MQ=11)

                                                                                 gtctgtCCCTCTGTCTCCATGCCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCt                                                             <  2:110001/138‑1 (MQ=11)

                                                                                   atgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTTCTTTGATTGGGATATACGTCTCATCAATACGCCATTTGTAATAAGTTTTTTTATACTTTTTCTTCCAAATTTGTAATCAAATTGGTGTATACTGTTg                                                           >  1:150286/1‑141 (MQ=11)

                                                                                     gtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATCAGCTTTTTTGTGCTTTTTCTTCCAAATTTGTTATAAAATTGGGGCATATTCTTTaa                                                         >  2:357683/1‑141 (MQ=11)

                                                                                       cccTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAAcc                                                       <  2:514914/141‑1 (MQ=255)

                                                                                       cccTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATAGTCTTGAAcc                                                       >  1:728880/1‑141 (MQ=21)

                                                                                         ctctGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATAAGTCTAATAAATACGCCAGTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAATTTTGATATAAAATTGGGACATATTCTTGAAcccc                                                     >  2:304/1‑140 (MQ=11)

                                                                                           ctgtaTCCATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAAc                                                   <  1:391292/137‑1 (MQ=255)

                                                                                           ctGTATCAATGGCACGATATAAATAGCTCCATTTTCCATTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAAc                                                   <  2:120050/141‑1 (MQ=255)

                                                                                                tCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAACATGGGGGCATATTCTTGGACCCAACGGTAg                                              >  2:239446/1‑141 (MQ=255)

                                                                                                tCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAg                                              <  1:473922/141‑1 (MQ=21)

                                                                                                tCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTATTGAACCCAACGGTAg                                              <  1:383275/141‑1 (MQ=255)

                                                                                                 aaaTGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTATAATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAACTTGATATTAAATTGGGGCATATTCTTGAACCAAACGGTata                                             >  1:140610/2‑139 (MQ=11)

                                                                                                   aTGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGGATCATCAATACGCCGTTAGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTTATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGatg                                           >  2:277120/1‑139 (MQ=11)

                                                                                                    tGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCg                                          <  1:100306/141‑1 (MQ=21)

                                                                                                      gCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCAATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAAACCAACGGCAAATCGtt                                        >  2:555579/1‑141 (MQ=11)

                                                                                                           atatAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGCatg                                   >  2:587874/1‑141 (MQ=255)

                                                                                                             ttaAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatgat                                 <  2:218803/140‑1 (MQ=255)

                                                                                                              taAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAatgatg                                >  2:143701/1‑141 (MQ=18)

                                                                                                               aaaTAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAAtgatga                               <  1:453905/141‑1 (MQ=255)

                                                                                                               aaaTAGCCCCATTTTCCTTTTATTTTGTTATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAAtgatga                               <  2:16615/141‑1 (MQ=11)

                                                                                                                 aTAGCTCCATTTTCCTTTTATTTTGATGTACGTATCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATGCAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAAc                             >  2:506892/1‑141 (MQ=255)

                                                                                                                 aTAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAAc                             >  2:635855/1‑141 (MQ=255)

                                                                                                                   aGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGATTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGt                           >  1:360311/1‑141 (MQ=255)

                                                                                                                         aTTTTCCTTTTGTGTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATACTTTTTCTTCCAAATTTGATATAAAATTTGGGCATATTCTTGAACCCAACGGTAGATCTTTGAATGATGAACGTTTacac                     <  1:397529/141‑1 (MQ=11)

                                                                                                                          ttttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGtggtgtaa                    <  2:249234/141‑8 (MQ=11)

                                                                                                                          tttcCCTTTTATTCTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacc                    <  2:406504/141‑1 (MQ=11)

                                                                                                                            ttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcaccac                  <  1:679871/141‑1 (MQ=39)

                                                                                                                            ttCCTTTTATTTGGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcaccac                  <  1:54098/141‑1 (MQ=255)

                                                                                                                             tCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTTTTTTTTTTCTTCCAAATTTGATATAAAATAGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGACGAACGTTTACCCCACg                 >  2:320274/1‑141 (MQ=11)

                                                                                                                              ccTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCGAACGGTAGACCGTTGAATGATGAACGTTTACACCACGt                >  1:319801/1‑141 (MQ=21)

                                                                                                                               cTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTGCACCACGtt               >  1:122485/1‑141 (MQ=255)

                                                                                                                                ttttATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTc              >  1:494868/1‑141 (MQ=255)

                                                                                                                                    tttttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCtt          <  2:440356/140‑1 (MQ=11)

                                                                                                                                    ttttgGATATAGGACTCATCAATACGCCTTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCtt          <  1:9985/140‑1 (MQ=11)

                                                                                                                                    aTTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCtt          <  1:598698/141‑1 (MQ=11)

                                                                                                                                    aTTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCtt          <  2:606290/141‑1 (MQ=11)

                                                                                                                                      tttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTCTGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTaa        <  1:672550/141‑1 (MQ=21)

                                                                                                                                       ttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAat       <  2:620471/141‑1 (MQ=11)

                                                                                                                                        tGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAata      <  2:480405/141‑1 (MQ=11)

TTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTC  >  CP000730/36175‑36455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`