Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 35,817 A→T 35.2% intergenic (+48/+60) USA300HOU_0025 → / ← USA300HOU_0026 hypothetical protein/IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073035,8170AT35.2% 163.3 / 100.8 110intergenic (+48/+60)USA300HOU_0025/USA300HOU_0026hypothetical protein/IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base A (40/30);  new base T (8/30);  total (49/61)
Fisher's exact test for biased strand distribution p-value = 4.82e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.04e-01

Only 100 of 111 total aligned reads displayed.

CAAATTATTCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATA  >  CP000730/35678‑35956
                                                                                                                                             |                                                                                                                                            
cAAATTATTCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAg                                                                                                                                             >  1:573131/1‑141 (MQ=255)
   attattCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGtt                                                                                                                                          <  1:685382/141‑1 (MQ=255)
     tattCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCt                                                                                                                                        >  2:557842/1‑141 (MQ=255)
                   aGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAGGGTTCTGTTGCAAAGTaaaa                                                                                                                          >  2:186880/1‑141 (MQ=255)
                          gatgatCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAAtata                                                                                                                   <  1:585347/141‑1 (MQ=255)
                          gatgatCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGAAAAAAGAAAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAAtata                                                                                                                   <  2:583935/141‑1 (MQ=255)
                           atgatCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAg                                                                                                                  >  1:273787/1‑141 (MQ=255)
                              atCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTa                                                                                                               >  2:70965/1‑141 (MQ=255)
                                   aTTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCAc                                                                                                          <  1:318002/141‑1 (MQ=255)
                                    ttGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTACACACt                                                                                                         >  2:114339/1‑141 (MQ=255)
                                    ttGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGATTTTGGCGAAAAATGATTACTTGAACAAGAACTAAAA‑‑TAAAATTGTGAGCTAATAAAAATAGAAGGTTCTGTTGCAAAGTACAAAAATATAGCTAACCACt                                                                                                         >  2:128784/1‑141 (MQ=255)
                                      gACAAAGTATTTAATGTAGCAGTGGTGCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTaa                                                                                                       >  2:165874/1‑141 (MQ=39)
                                      gACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAACAATGATTACTTGAACAAGCGCCAACAGCTGAAATTGGACAAAGGAAAATACAAGGGACTGTTGCAAAGTAAAAAAAGATAGCTAACCACaaa                                                                                                       >  2:734231/1‑141 (MQ=255)
                                       aCAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTGTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAGAAAATATAGCTAACCACTAAt                                                                                                      >  1:187466/1‑141 (MQ=255)
                                          aaGTATTTAATGTAGAAGCTATTGAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTa                                                                                                   >  2:548819/1‑141 (MQ=255)
                                          aaGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTCAtttt                                                                                                   >  2:226169/1‑140 (MQ=255)
                                          aaGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTa                                                                                                   >  1:534413/1‑141 (MQ=255)
                                          aaGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACAACTAATTTa                                                                                                   >  1:431520/1‑141 (MQ=255)
                                            gTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATc                                                                                                 >  1:187510/1‑141 (MQ=255)
                                              aTTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCAt                                                                                               <  1:414221/141‑1 (MQ=255)
                                               tttAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAGAAATGATTACTTGAACAAGAGCTAACA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTGTGTTGCAACGTAAAAAAATATAGATAACCAGTAATTTATTATg                                                                                              >  2:617607/1‑141 (MQ=255)
                                               tttAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATg                                                                                              >  1:374363/1‑141 (MQ=255)
                                                 tAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTAGCATGTc                                                                                            <  2:424938/141‑1 (MQ=255)
                                                  aaTGTAGAAGCTATTGAGTATAAAGCTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCa                                                                                           >  1:386021/1‑141 (MQ=255)
                                                  aaTGGAGAAGCTAATCAGTATAAAGGTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTAATAAAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCa                                                                                           <  2:417546/141‑1 (MQ=37)
                                                      tAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAgtgt                                                                                       <  1:161337/141‑1 (MQ=255)
                                                        gAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGCGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTCTTGCAATGTAAAAAAATATGGCTAACCACTAATGTATCATGACAGCGTTc                                                                                     >  1:78298/1‑141 (MQ=255)
                                                          aGCTACTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCGGTTGCAAAGTAAAAAAATATAGCTAACCTCTAATTTATCATGTCAGTGTTCGc                                                                                   <  1:145417/141‑1 (MQ=255)
                                                           gCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCt                                                                                  >  1:592981/1‑141 (MQ=255)
                                                              aTTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCAAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTACTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATATAAGTGTTCACATaa                                                                               >  2:713768/1‑141 (MQ=37)
                                                                 cAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACtt                                                                            >  2:403176/1‑141 (MQ=255)
                                                                   gTATAAAGTTTTTGGCGAAAAATGATTAGTTGAACAAGAGCTAATA‑‑GAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGc                                                                          <  2:113515/141‑1 (MQ=255)
                                                                                  cGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCatgatgatcac                                                           >  2:377117/1‑136 (MQ=255)
                                                                                     aaaaTGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTc                                                       <  1:404544/141‑1 (MQ=255)
                                                                                       aaTGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGt                                                     <  2:744828/141‑1 (MQ=255)
                                                                                        aTGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTg                                                    <  1:125692/141‑1 (MQ=255)
                                                                                          gATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGc                                                  >  1:117231/1‑141 (MQ=255)
                                                                                          gATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGc                                                  >  1:367499/1‑141 (MQ=255)
                                                                                          gATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATACTAATTTCTTGGc                                                  >  1:285700/1‑141 (MQ=255)
                                                                                           aTTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCa                                                 >  1:147266/1‑141 (MQ=255)
                                                                                               cTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGc                                             <  1:283214/141‑1 (MQ=255)
                                                                                                       agagCTAAAA‑‑TAAAATTGTGGTCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATcc                                     >  2:14839/1‑141 (MQ=255)
                                                                                                        gagCTAAAA‑‑TAAAACTGGGATCTAATAAAAATCGAAGGTTCTGTTGCAACGACAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACGTGCTAGCATGATGCTACTTACGTGGccgtgcgagactccg                                    >  2:172539/1‑127 (MQ=17)
                                                                                                          gCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTGGCATGCTGCCCAATCTCTGGCATGGCGAAAATCCGTa                                  <  1:184987/141‑1 (MQ=18)
                                                                                                              aaa‑‑taaaatTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGCTc                              >  1:170029/1‑141 (MQ=255)
                                                                                                              aaa‑‑taaaatTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATc                              >  1:94181/1‑141 (MQ=255)
                                                                                                                  acaaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCCGaa                          <  1:245252/127‑1 (MQ=11)
                                                                                                                   taaaatTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATACCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGa                           >  1:146200/1‑141 (MQ=255)
                                                                                                                    aaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAaga                        <  2:325815/129‑1 (MQ=11)
                                                                                                                     aaatTGTTATCTAATAAAAATAGAAGTCGCTGTGGCAAAGTAACAAAATATAGCTAACCACTAATTTATCATGTCAGTGTCCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAag                         <  2:85341/141‑1 (MQ=14)
                                                                                                                     aaatTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAag                         <  1:669591/141‑1 (MQ=255)
                                                                                                                     aaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAagag                       <  1:741271/130‑1 (MQ=11)
                                                                                                                       atTGTGATCTAATAAAAATAGAAGGGTTTGTTGAAAAGAAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAagag                       <  2:273787/141‑1 (MQ=255)
                                                                                                                       agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                     <  1:102241/132‑1 (MQ=11)
                                                                                                                       agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                     <  2:461944/132‑1 (MQ=11)
                                                                                                                       agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                     <  1:104734/132‑1 (MQ=11)
                                                                                                                       agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                     <  1:452464/132‑1 (MQ=11)
                                                                                                                       agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                     <  1:640564/132‑1 (MQ=11)
                                                                                                                       agagtagttgaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                     <  2:374421/131‑1 (MQ=11)
                                                                                                                        gagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                    <  1:98840/133‑1 (MQ=11)
                                                                                                                        gagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                    <  2:240880/133‑1 (MQ=11)
                                                                                                                        gagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGATAATCACTAATATATCCTGTCAGTGTTCGTTTAACTTACTAGCATACTGCTACTTTCGGGGCATGGCGAAAATCCGTAGATATGAAGAAAcc                    >  2:747799/9‑141 (MQ=2)
                                                                                                                         tgtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                     >  2:306295/1‑141 (MQ=255)
                                                                                                                          gtgATCTAATACAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                    <  1:170459/141‑1 (MQ=255)
                                                                                                                          gtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                  >  2:529666/7‑141 (MQ=11)
                                                                                                                          gtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGATAATCACTAATATATAATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                  >  1:108390/7‑141 (MQ=11)
                                                                                                                          gtattttaataaAAACGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCGAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                  <  1:23048/135‑1 (MQ=11)
                                                                                                                          gtattttaataaAAACGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACATGCTAGCATGATGTTAATTTCGTGGCATGGCGAAAATTCGTAGATCTGAAGAGACCTg                  <  1:466066/135‑1 (MQ=11)
                                                                                                                           tattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAACATCCGTAGATCTGAAGAGACCTGc                 >  2:329494/6‑141 (MQ=11)
                                                                                                                           tattttaataaAAATGATGGTTCTGCAGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                 <  1:587785/136‑1 (MQ=11)
                                                                                                                           gattttaataaAAACGATGGGTCTTTTGCAAAGTAAAAAACATAGCTAATCACGAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                 <  1:347449/136‑1 (MQ=11)
                                                                                                                            gATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAACTCAGTAGATCTAAAGAGACCTg                  >  2:720003/1‑141 (MQ=255)
                                                                                                                            gATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGATGAGACCTg                  >  2:525534/1‑141 (MQ=255)
                                                                                                                            gATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                  >  1:634764/1‑141 (MQ=255)
                                                                                                                            attttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAATTCCGTAGATCTGAAGAGACCTGCg                <  2:589830/137‑1 (MQ=11)
                                                                                                                            attttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                <  1:417923/137‑1 (MQ=11)
                                                                                                                             ttttaataaACATGATGGTTATGTTGCAGAGTAAAAAATATAGCTAATCACTAATATATAATGTCAGTGTGCGTTTAACTTGCTAGCATTATGCTATTTTCGTGGCATGGCGAAAATCAGTAGATCTGAAGATACCTGCgg               >  2:238573/4‑141 (MQ=11)
                                                                                                                             ttttaataaAAATGATTGTTCTGTTGCAAATTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg               <  2:705854/138‑1 (MQ=11)
                                                                                                                             ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg               <  1:298128/138‑1 (MQ=11)
                                                                                                                             ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg               >  2:326033/4‑141 (MQ=11)
                                                                                                                             ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg               <  1:71200/138‑1 (MQ=11)
                                                                                                                             ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg               <  2:54549/138‑1 (MQ=11)
                                                                                                                             ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCGAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg               >  1:748206/4‑141 (MQ=11)
                                                                                                                             aTCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                 <  1:130404/141‑1 (MQ=255)
                                                                                                                              ttaaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCCCTCATACATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt              <  1:260537/138‑1 (MQ=11)
                                                                                                                              tCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                <  1:19658/141‑1 (MQ=255)
                                                                                                                               ttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCTGTAGATCTGAAGAGACCTGCGGtt             <  1:717824/140‑1 (MQ=11)
                                                                                                                               ttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGtt             <  2:41180/140‑1 (MQ=11)
                                                                                                                               ttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGtt             <  1:399858/140‑1 (MQ=11)
                                                                                                                               ttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCAGtt             <  1:551527/140‑1 (MQ=11)
                                                                                                                                 aataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAGCCCGTAGATCTGAAGAGACCTGCGGTTCt           >  2:77445/1‑141 (MQ=11)
                                                                                                                                 aataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCt           <  2:541797/141‑1 (MQ=11)
                                                                                                                                 aataaAAATGATGGTTCTGTCGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCt           <  2:578283/141‑1 (MQ=11)
                                                                                                                                 aataaAAAGAGAAGGTTATGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGtt             <  2:224697/141‑1 (MQ=255)
                                                                                                                                  ataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCtt          <  1:581494/141‑1 (MQ=11)
                                                                                                                                  ataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCtt          <  2:624017/141‑1 (MQ=11)
                                                                                                                                       aaTAGAAGGCTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCttttt       <  1:239082/141‑1 (MQ=21)
                                                                                                                                         tAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtat     <  1:165874/141‑1 (MQ=32)
                                                                                                                                          aGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTATCGTAACGTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtata    <  1:557842/141‑1 (MQ=255)
                                                                                                                                            aaGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCCGAAGAGACCTGCGGTTCTTTTtatata  <  1:219886/141‑1 (MQ=11)
                                                                                                                                             |                                                                                                                                            
CAAATTATTCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATA  >  CP000730/35678‑35956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: