BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,471	A→G	18.3%	S27S (AGT→AGC)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,471	0	A	G	18.3%	220.8 / 47.1	120	S27S (AGT→AGC)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base A (46/52); new base G (10/12); total (56/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.67e-01

Only 100 of 126 total aligned reads displayed.

CGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTAT  >  CP000730/36331‑36611

cGCCATTTGTAATGAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATaa                                                                                                                                              <  1:1837/141‑1 (MQ=17)

 gCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAAc                                                                                                                                             <  1:544342/141‑1 (MQ=21)

 cccATCTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAAc                                                                                                                                             <  1:370346/140‑1 (MQ=21)

  ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCt                                                                                                                                            >  1:476484/1‑141 (MQ=11)

  ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCt                                                                                                                                            >  2:680991/1‑141 (MQ=255)

  ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAAcc                                                                                                                                            >  1:761214/1‑140 (MQ=21)

  ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACt                                                                                                                                            >  1:423351/1‑141 (MQ=25)

   cctttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGCTATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACTCCACGTTCTCTTAATATTTCAGATATATCACGATAGCTc                                                                                                                                           <  2:323795/139‑1 (MQ=11)

   cctttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTc                                                                                                                                           <  2:551712/139‑1 (MQ=11)

   cATTTGTAATAAGCTTTTTTATGCTTTTTTTTTCTTTTTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACGGTTGAATGATGAACCTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACtt                                                                                                                                           >  2:78169/1‑140 (MQ=11)

   cATTTGTAATAAGCTGTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAGTGATGAACGTTTACTCCACGTTCCCTTAATATTTCAGATATATCACGATAACTc                                                                                                                                           <  2:755141/141‑1 (MQ=255)

   cATTTATAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATAGAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTc                                                                                                                                           <  1:367767/141‑1 (MQ=11)

     tttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCaa                                                                                                                                         >  1:695621/1‑141 (MQ=11)

     tttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCaa                                                                                                                                         <  1:258988/141‑1 (MQ=18)

     tttGTAATAAGCTTTTTTATGCTTTTTCGTCCAAATTTGATATAAAAGTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCaa                                                                                                                                         <  2:167940/141‑1 (MQ=11)

     tgtGTAATAAGCTTTTTTATGTTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAACTCaa                                                                                                                                         <  1:595163/139‑1 (MQ=255)

      ttGTAATAAGCTTTTTTATGCTTTTTTTTTTTTTTTTTTTATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAAATATATCACTATTGCTCatt                                                                                                                                        >  2:485333/1‑139 (MQ=11)

      ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAAt                                                                                                                                        <  2:599485/141‑1 (MQ=255)

      ttGTAATAAGCTTTTTTATACTTTTTCTTCCAATTTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAAt                                                                                                                                        >  1:409006/1‑141 (MQ=11)

       tGTATTAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATAACCTAGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATg                                                                                                                                       <  2:486723/141‑1 (MQ=11)

       tGTAATATGCTTTTTTATGCTTTTTCTTTCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATg                                                                                                                                       <  1:251246/141‑1 (MQ=255)

       tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACTGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAAGATTTCAGATATATCACGATAACTCAATg                                                                                                                                       <  1:481338/141‑1 (MQ=255)

       tGTAATAAGCTTTTTTATGCTTTGTCCTCCAACTTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATg                                                                                                                                       <  1:618393/141‑1 (MQ=11)

       tGTAATAAGCGTTTTTATGCCTTTTCGTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATg                                                                                                                                       <  2:678825/141‑1 (MQ=11)

        gTAATAAGCTTTTTTATGCTTTTTCTTCCACACTTGATATCAATTTGGGTCATATTCTTGAACCCAATGAGGGATGGTTCACAGTTTTGAAGTTACTCCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgc                                                                                                                                      <  1:754291/141‑2 (MQ=2)

        gTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATgc                                                                                                                                      >  2:425613/1‑140 (MQ=18)

         taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAATTACATGCACATTCTTGGACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATAGTTCCGATATATCATGATAACTCAATgca                                                                                                                                     <  2:538106/141‑3 (MQ=11)

         taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                                                                                                                     <  1:241130/141‑3 (MQ=11)

         taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                                                                                                                     <  2:753103/141‑3 (MQ=11)

         taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTAAATgca                                                                                                                                     >  1:435072/1‑139 (MQ=11)

         taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATgca                                                                                                                                     <  1:753447/141‑3 (MQ=17)

          aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCCCGATAGCTCCATgcct                                                                                                                                    >  1:102734/1‑138 (MQ=11)

          aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATGaat                                                                                                                                    >  2:377731/1‑141 (MQ=11)

             aaGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATc                                                                                                                                 >  1:228653/1‑141 (MQ=38)

                      tATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGAtagt                                                                                                                        >  2:35258/1‑141 (MQ=34)

                         gCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGc                                                                                                                     >  2:134848/1‑141 (MQ=35)

                         gCTTTTTCTTCCAAATTTGATACAAAATCGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGc                                                                                                                     >  1:495119/1‑141 (MQ=25)

                          cTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGcg                                                                                                                    >  1:647411/1‑140 (MQ=21)

                              ttcttcCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACg                                                                                                                <  1:134848/141‑1 (MQ=35)

                                cttcCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGc                                                                                                              <  1:384518/141‑1 (MQ=35)

                                   ccAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTATCCAACGGCTAc                                                                                                           <  2:168121/141‑1 (MQ=25)

                                          tGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATa                                                                                                    <  1:506892/141‑1 (MQ=35)

                                           gATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACATCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATaa                                                                                                   >  2:727337/1‑141 (MQ=25)

                                            aTACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATAACGATAACTCAATGTATAACTTAGATAGTAGCCAACGGCTCCAGTGTTAAc                                                                                                  >  1:175958/1‑141 (MQ=255)

                                              aCAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACac                                                                                                >  1:363742/1‑140 (MQ=18)

                                                   aTTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCAAACGGCTACAGTGATAACATCCttt                                                                                           >  2:71592/1‑140 (MQ=17)

                                                    ttGGGGCATATTCTTGACCCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGt                                                                                          <  1:451276/141‑1 (MQ=17)

                                                    ttGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGt                                                                                          <  2:187890/141‑1 (MQ=32)

                                                      ggggCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTa                                                                                        >  2:453354/1‑141 (MQ=32)

                                                          cATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATACTGGTTAAAtt                                                                                    >  1:543031/1‑141 (MQ=17)

                                                            tatTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGt                                                                                  <  1:431976/141‑1 (MQ=32)

                                                                cTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTtat                                                                              >  1:135965/1‑141 (MQ=32)

                                                                 ttGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTtata                                                                             <  2:341040/141‑1 (MQ=32)

                                                                 ttGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTtata                                                                             >  1:248243/1‑141 (MQ=32)

                                                                 ttGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTtata                                                                             <  1:302797/141‑1 (MQ=32)

                                                                   gaccccAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATc                                                                           <  2:596757/138‑1 (MQ=17)

                                                                        cAACGGTAGACCGTTGAATTATGAACGTTTACACCGCGTTCCCTCATTATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGaaa                                                                      <  2:747462/141‑1 (MQ=255)

                                                                        cAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGaaa                                                                      >  1:267058/1‑141 (MQ=32)

                                                                        cAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGaaa                                                                      <  1:297646/141‑1 (MQ=32)

                                                                            ggtctaCCGTTGGATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGt                                                                  >  2:46287/6‑141 (MQ=12)

                                                                              tctaCCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGtta                                                                >  2:425417/4‑140 (MQ=17)

                                                                                gACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCAt                                                              >  2:25809/1‑141 (MQ=32)

                                                                                   cGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACa                                                           <  2:360401/141‑1 (MQ=32)

                                                                                     ttGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACaga                                                         <  1:297053/141‑1 (MQ=32)

                                                                                       gtgtgatgcACCTTTAGACCACGTGTCTTTGATTTTTCAGGGATATGATGATAACTTAGTGTATGTCTTCGATAGGGGCCCACGGCTACAGGGATAACATCCTGGTTAAATTGTTTATATCTGAAATAGTTCATACagaag                                                       <  2:597678/138‑1 (MQ=2)

                                                                                          tgatgaACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACt                                                    <  2:91867/141‑1 (MQ=32)

                                                                                          tgatgaACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACt                                                    <  1:13135/141‑1 (MQ=32)

                                                                                            atgaACGTTTACACCACGTTCAATGGTGTCAGCATATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCGACGGCTACAGTGATAACATCCTTGTTAACTTGTTTATATGTGAAATAGTTCTTCCAGAAGACTcc                                                  >  1:184189/1‑141 (MQ=11)

                                                                                                aCGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCtttt                                              >  2:249089/1‑141 (MQ=32)

                                                                                                 cGTTTACATCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCttttt                                             <  1:727337/141‑1 (MQ=17)

                                                                                                 cGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCttttt                                             <  2:134192/141‑1 (MQ=32)

                                                                                                 cGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCttttt                                             <  2:134093/141‑1 (MQ=32)

                                                                                                  gTTTACACCACGTTCCCTTAATATTTCAGTTATATCACGACAACTCAAGGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGATTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTg                                            <  2:248243/141‑1 (MQ=255)

                                                                                                  gTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTg                                            >  2:510075/1‑141 (MQ=32)

                                                                                                    ttACACCACGTTCCCTTAATATTTCATATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCAAACAGAAGACTCCTTTTTGtt                                          <  1:465239/141‑1 (MQ=17)

                                                                                                    tagcaccacGTTCACTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCTAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGtt                                          <  2:570136/138‑1 (MQ=12)

                                                                                                     gtgtaaacGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTa                                         >  2:539844/7‑141 (MQ=17)

                                                                                                      tgtaaacGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTaa                                        >  2:1732/6‑141 (MQ=17)

                                                                                                       caccacGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTCGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTaaa                                       >  1:483533/1‑141 (MQ=17)

                                                                                                        accacGTTCACTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAAGGTAATACCACTGAAAGAGTTCATACAGAAGACTCCTTTTTGTTaaaa                                      <  1:369614/141‑1 (MQ=11)

                                                                                                          cgcGTTCCCTTTATATTTCAGATATATCTCGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAAtt                                    <  2:432688/139‑1 (MQ=12)

                                                                                                           acGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATta                                   >  1:652610/1‑141 (MQ=32)

                                                                                                              ttCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGGTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATAc                                >  1:724255/1‑141 (MQ=17)

                                                                                                                     aaTATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAAt                         <  1:650688/141‑1 (MQ=35)

                                                                                                                      atgtgtCAGATATATCACGATAACTACATGAATATCTTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAAtt                        <  2:485960/136‑1 (MQ=11)

                                                                                                                            cAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACtt                  >  1:93647/1‑141 (MQ=35)

                                                                                                                             aGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACttt                 <  1:578819/141‑1 (MQ=35)

                                                                                                                                  tatCCCGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAAc            <  1:207680/141‑1 (MQ=25)

                                                                                                                                  tatCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCaaa            >  2:402712/1‑140 (MQ=21)

                                                                                                                                  tatCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAAc            >  1:745558/1‑141 (MQ=35)

                                                                                                                                  tatCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAAc            >  1:484238/1‑141 (MQ=35)

                                                                                                                                     cACGATAACTCAATTTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGa         <  1:336527/141‑1 (MQ=25)

                                                                                                                                     cACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGATGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGa         <  2:492918/141‑1 (MQ=25)

                                                                                                                                      aCGCTAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGaa        >  1:552389/1‑141 (MQ=25)

                                                                                                                                         aTAGCTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCg     <  2:445236/141‑1 (MQ=9)

                                                                                                                                          tAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGt    >  2:8100/1‑141 (MQ=37)

                                                                                                                                          caaCGCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTACAACAGAACCGt    >  2:702215/2‑141 (MQ=255)

                                                                                                                                           aaCTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGta   >  1:221879/1‑141 (MQ=37)

                                                                                                                                            aCTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGtat  <  1:17649/141‑1 (MQ=38)

                                                                                                                                            aCTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGtat  >  1:635992/1‑141 (MQ=39)

CGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTAT  >  CP000730/36331‑36611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`