Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 35,850 C→T 38.9% intergenic (+81/+27) USA300HOU_0025 → / ← USA300HOU_0026 hypothetical protein/IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073035,8500CT38.9% 137.2 / 90.6 103intergenic (+81/+27)USA300HOU_0025/USA300HOU_0026hypothetical protein/IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base C (34/28);  new base T (9/31);  total (44/59)
Fisher's exact test for biased strand distribution p-value = 1.89e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 103 total aligned reads displayed.

ATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATT  >  CP000730/35713‑35971
                                                                                                                                           |                                                                                                                          
aTTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCAc                                                                                                                         <  1:318002/141‑1 (MQ=255)
 ttGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTACACACt                                                                                                                        >  2:114339/1‑141 (MQ=255)
 ttGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGATTTTGGCGAAAAATGATTACTTGAACAAGAACTAAAA‑‑TAAAATTGTGAGCTAATAAAAATAGAAGGTTCTGTTGCAAAGTACAAAAATATAGCTAACCACt                                                                                                                        >  2:128784/1‑141 (MQ=255)
   gACAAAGTATTTAATGTAGCAGTGGTGCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTaa                                                                                                                      >  2:165874/1‑141 (MQ=39)
   gACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAACAATGATTACTTGAACAAGCGCCAACAGCTGAAATTGGACAAAGGAAAATACAAGGGACTGTTGCAAAGTAAAAAAAGATAGCTAACCACaaa                                                                                                                      >  2:734231/1‑141 (MQ=255)
    aCAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTGTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAGAAAATATAGCTAACCACTAAt                                                                                                                     >  1:187466/1‑141 (MQ=255)
       aaGTATTTAATGTAGAAGCTATTGAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTa                                                                                                                  >  2:548819/1‑141 (MQ=255)
       aaGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTCAtttt                                                                                                                  >  2:226169/1‑140 (MQ=255)
       aaGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTa                                                                                                                  >  1:534413/1‑141 (MQ=255)
       aaGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACAACTAATTTa                                                                                                                  >  1:431520/1‑141 (MQ=255)
         gTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATc                                                                                                                >  1:187510/1‑141 (MQ=255)
           aTTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCAt                                                                                                              <  1:414221/141‑1 (MQ=255)
            tttAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAGAAATGATTACTTGAACAAGAGCTAACA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTGTGTTGCAACGTAAAAAAATATAGATAACCAGTAATTTATTATg                                                                                                             >  2:617607/1‑141 (MQ=255)
            tttAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATg                                                                                                             >  1:374363/1‑141 (MQ=255)
              tAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTAGCATGTc                                                                                                           <  2:424938/141‑1 (MQ=255)
               aaTGTAGAAGCTATTGAGTATAAAGCTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCa                                                                                                          >  1:386021/1‑141 (MQ=255)
               aaTGGAGAAGCTAATCAGTATAAAGGTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTAATAAAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCa                                                                                                          <  2:417546/141‑1 (MQ=37)
                   tAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAgtgt                                                                                                      <  1:161337/141‑1 (MQ=255)
                     gAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGCGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTCTTGCAATGTAAAAAAATATGGCTAACCACTAATGTATCATGACAGCGTTc                                                                                                    >  1:78298/1‑141 (MQ=255)
                       aGCTACTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCGGTTGCAAAGTAAAAAAATATAGCTAACCTCTAATTTATCATGTCAGTGTTCGc                                                                                                  <  1:145417/141‑1 (MQ=255)
                        gCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCt                                                                                                 >  1:592981/1‑141 (MQ=255)
                           aTTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCAAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTACTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATATAAGTGTTCACATaa                                                                                              >  2:713768/1‑141 (MQ=37)
                              cAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACtt                                                                                           >  2:403176/1‑141 (MQ=255)
                               aGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTg                                                                                          >  1:631679/1‑141 (MQ=255)
                                gTATAAAGTTTTTGGCGAAAAATGATTAGTTGAACAAGAGCTAATA‑‑GAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGc                                                                                         <  2:113515/141‑1 (MQ=255)
                                        tttttGGCGAAAAATGATTACTTGAACCAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTACTTTATCATGTCAGTGTTCGCTTAACTTGCTAGCatga                                                                                 >  2:89857/1‑141 (MQ=255)
                                               cGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCatgatgatcac                                                                          >  2:377117/1‑136 (MQ=255)
                                                  aaaaTGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTc                                                                      <  1:404544/141‑1 (MQ=255)
                                                    aaTGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGt                                                                    <  2:744828/141‑1 (MQ=255)
                                                     aTGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTg                                                                   <  1:125692/141‑1 (MQ=255)
                                                      tGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTgg                                                                  >  1:238577/1‑141 (MQ=255)
                                                       gATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGc                                                                 >  1:117231/1‑141 (MQ=255)
                                                       gATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGc                                                                 >  1:367499/1‑141 (MQ=255)
                                                       gATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATACTAATTTCTTGGc                                                                 >  1:285700/1‑141 (MQ=255)
                                                        aTTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCa                                                                >  1:147266/1‑141 (MQ=255)
                                                            cTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGc                                                            <  1:283214/141‑1 (MQ=255)
                                                            cTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGc                                                            <  1:161676/141‑1 (MQ=255)
                                                            attaaaaaaGAGCTAAAA‑‑TAAAATTGTTATCTAAAAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGc                                                            <  2:534380/134‑1 (MQ=255)
                                                                    agagCTAAAA‑‑TAAAATTGTGGTCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATcc                                                    >  2:14839/1‑141 (MQ=255)
                                                                     gagCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCg                                                   <  2:210447/141‑1 (MQ=255)
                                                                     gagCTAAAA‑‑TAAAACTGGGATCTAATAAAAATCGAAGGTTCTGTTGCAACGACAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACGTGCTAGCATGATGCTACTTACGTGGccgtgcgagactccg                                                   >  2:172539/1‑127 (MQ=17)
                                                                      agCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAGAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAAGTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGt                                                  >  2:336004/1‑141 (MQ=255)
                                                                       gCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTGGCATGCTGCCCAATCTCTGGCATGGCGAAAATCCGTa                                                 <  1:184987/141‑1 (MQ=18)
                                                                           aaa‑‑taaaatTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGCTc                                             >  1:170029/1‑141 (MQ=255)
                                                                           aaa‑‑taaaatTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATc                                             >  1:94181/1‑141 (MQ=255)
                                                                               acaaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCCGaa                                         <  1:245252/127‑1 (MQ=11)
                                                                                taaaatTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATACCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGa                                          >  1:146200/1‑141 (MQ=255)
                                                                                 aaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAaga                                       <  2:325815/129‑1 (MQ=11)
                                                                                  aaatTGTTATCTAATAAAAATAGAAGTCGCTGTGGCAAAGTAACAAAATATAGCTAACCACTAATTTATCATGTCAGTGTCCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAag                                        <  2:85341/141‑1 (MQ=14)
                                                                                  aaatTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAag                                        <  1:669591/141‑1 (MQ=255)
                                                                                  aaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAagag                                      <  1:741271/130‑1 (MQ=11)
                                                                                    atTGTGATCTAATAAAAATAGAAGGGTTTGTTGAAAAGAAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAagag                                      <  2:273787/141‑1 (MQ=255)
                                                                                    agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                    <  1:102241/132‑1 (MQ=11)
                                                                                    agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                    <  2:461944/132‑1 (MQ=11)
                                                                                    agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                    <  1:104734/132‑1 (MQ=11)
                                                                                    agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                    <  1:640564/132‑1 (MQ=11)
                                                                                    agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                    <  1:452464/132‑1 (MQ=11)
                                                                                    agagtagttgaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                    <  2:374421/131‑1 (MQ=11)
                                                                                     gagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                                   <  1:98840/133‑1 (MQ=11)
                                                                                     gagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGATAATCACTAATATATCCTGTCAGTGTTCGTTTAACTTACTAGCATACTGCTACTTTCGGGGCATGGCGAAAATCCGTAGATATGAAGAAAcc                                   >  2:747799/9‑141 (MQ=2)
                                                                                      tgtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                    >  2:306295/1‑141 (MQ=255)
                                                                                       gtgATCTAATACAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                                   <  1:170459/141‑1 (MQ=255)
                                                                                       gtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                                   <  1:359196/141‑1 (MQ=255)
                                                                                       gtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                                 >  2:529666/7‑141 (MQ=11)
                                                                                       gtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGATAATCACTAATATATAATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                                 >  1:108390/7‑141 (MQ=11)
                                                                                       gtattttaataaAAACGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCGAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                                 <  1:23048/135‑1 (MQ=11)
                                                                                       gtattttaataaAAACGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACATGCTAGCATGATGTTAATTTCGTGGCATGGCGAAAATTCGTAGATCTGAAGAGACCTg                                 <  1:466066/135‑1 (MQ=11)
                                                                                        tattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAACATCCGTAGATCTGAAGAGACCTGc                                >  2:329494/6‑141 (MQ=11)
                                                                                        tattttaataaAAATGATGGTTCTGCAGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                <  1:587785/136‑1 (MQ=11)
                                                                                        gattttaataaAAACGATGGGTCTTTTGCAAAGTAAAAAACATAGCTAATCACGAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                <  1:347449/136‑1 (MQ=11)
                                                                                         gATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAACTCAGTAGATCTAAAGAGACCTg                                 >  2:720003/1‑141 (MQ=255)
                                                                                         gATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGATGAGACCTg                                 >  2:525534/1‑141 (MQ=255)
                                                                                         gATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                                 >  1:634764/1‑141 (MQ=255)
                                                                                         attttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAATTCCGTAGATCTGAAGAGACCTGCg                               <  2:589830/137‑1 (MQ=11)
                                                                                         attttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                               <  1:417923/137‑1 (MQ=11)
                                                                                          ttttaataaACATGATGGTTATGTTGCAGAGTAAAAAATATAGCTAATCACTAATATATAATGTCAGTGTGCGTTTAACTTGCTAGCATTATGCTATTTTCGTGGCATGGCGAAAATCAGTAGATCTGAAGATACCTGCgg                              >  2:238573/4‑141 (MQ=11)
                                                                                          ttttaataaAAATGATTGTTCTGTTGCAAATTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                              <  2:705854/138‑1 (MQ=11)
                                                                                          ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                              <  2:54549/138‑1 (MQ=11)
                                                                                          ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                              >  2:326033/4‑141 (MQ=11)
                                                                                          ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                              <  1:298128/138‑1 (MQ=11)
                                                                                          ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                              <  1:71200/138‑1 (MQ=11)
                                                                                          ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCGAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                              >  1:748206/4‑141 (MQ=11)
                                                                                          aTCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                <  1:130404/141‑1 (MQ=255)
                                                                                           ttaaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCCCTCATACATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt                             <  1:260537/138‑1 (MQ=11)
                                                                                           tCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                               <  1:19658/141‑1 (MQ=255)
                                                                                            ttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCTGTAGATCTGAAGAGACCTGCGGtt                            <  1:717824/140‑1 (MQ=11)
                                                                                            ttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGtt                            <  2:41180/140‑1 (MQ=11)
                                                                                            ttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCAGtt                            <  1:551527/140‑1 (MQ=11)
                                                                                              aataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAGCCCGTAGATCTGAAGAGACCTGCGGTTCt                          >  2:77445/1‑141 (MQ=11)
                                                                                              aataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCt                          <  2:541797/141‑1 (MQ=11)
                                                                                              aataaAAATGATGGTTCTGTCGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCt                          <  2:578283/141‑1 (MQ=11)
                                                                                              aataaAAAGAGAAGGTTATGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGtt                            <  2:224697/141‑1 (MQ=255)
                                                                                               ataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCtt                         <  1:581494/141‑1 (MQ=11)
                                                                                               ataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCtt                         <  2:624017/141‑1 (MQ=11)
                                                                                                    aaTAGAAGGCTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCttttt                      <  1:239082/141‑1 (MQ=21)
                                                                                                     aTAGACGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACGTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTta                     <  2:656717/141‑1 (MQ=17)
                                                                                                      tAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtat                    <  1:165874/141‑1 (MQ=32)
                                                                                                       aGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTATCGTAACGTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtata                   <  1:557842/141‑1 (MQ=255)
                                                                                                         aaGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCCGAAGAGACCTGCGGTTCTTTTtatata                 <  1:219886/141‑1 (MQ=11)
                                                                                                             ttcttgtGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTCGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCg            <  1:218007/135‑1 (MQ=1)
                                                                                                                                           |                                                                                                                          
ATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAA‑‑TAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATT  >  CP000730/35713‑35971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: