Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 110,660 T→A 8.4% I173I (ATT→ATA USA300HOU_0109 → staphylococcal tandem lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730110,6600TA 8.4% 202.7 / 11.9 72I173I (ATT→ATAUSA300HOU_0109staphylococcal tandem lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (25/40);  new base A (4/2);  total (29/43)
Fisher's exact test for biased strand distribution p-value = 2.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.31e-01

CCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTCAGGGAATT  >  CP000730/110520‑110799
                                                                                                                                            |                                                                                                                                           
ccGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGAGGGAGATAtt                                                                                                                                             >  1:289468/1‑141 (MQ=255)
 cGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAttt                                                                                                                                            <  1:5727/141‑1 (MQ=255)
 cGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAttt                                                                                                                                            <  1:30260/141‑1 (MQ=255)
 cGGTTAAAATGGTCGATAATAAAATCATTCAAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTATAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACAGAGATAttt                                                                                                                                            >  2:7468/1‑141 (MQ=255)
          tGGTCGATAATAAAATAATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAAGCGAAAACTTTAAGTTATTTGTTCAATCTGGGGCATTTAAAGATTTGCAAAATTATAAGGTAGGAGATATTTAatcaagtt                                                                                                                                   >  1:4852/1‑135 (MQ=14)
           ggTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATca                                                                                                                                  >  2:173950/1‑140 (MQ=255)
             tCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTATGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCag                                                                                                                                <  2:655460/141‑1 (MQ=255)
             gcGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGACAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCag                                                                                                                                <  2:273923/140‑1 (MQ=255)
              cGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCaga                                                                                                                               >  2:681973/1‑141 (MQ=255)
               gATAATAAAATCATTACAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCagag                                                                                                                              <  2:346056/141‑1 (MQ=255)
                    taaAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGAGTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGcc                                                                                                                         >  2:446031/1‑141 (MQ=255)
                    taaAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGcc                                                                                                                         <  1:364283/141‑1 (MQ=255)
                     aaaaCCATTTCAACAAAAGAAATTAAAAATTCAAATATAAAAAAAGAAATCGAAAACTTTAAGTTCTATGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCg                                                                                                                        <  1:210373/141‑1 (MQ=17)
                      aaaTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAATCTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGa                                                                                                                       <  2:244062/141‑1 (MQ=255)
                       aaTGATTCCAACAGAAGAAATTAATGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAg                                                                                                                      <  2:174000/141‑1 (MQ=255)
                        aTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGt                                                                                                                     >  2:440788/1‑141 (MQ=255)
                          cATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTa                                                                                                                   <  1:446031/141‑1 (MQ=255)
                              ccAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCg                                                                                                               >  2:588755/1‑141 (MQ=255)
                                aacaaAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGc                                                                                                             <  1:487328/141‑1 (MQ=255)
                                aacaaAAGAAATGAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGc                                                                                                             <  1:705225/141‑1 (MQ=255)
                                      aGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAata                                                                                                       <  1:743079/141‑1 (MQ=255)
                                         aaTTAAAGATGAAAAAATAAAAAAAGAGATGGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCa                                                                                                    >  1:757315/1‑141 (MQ=255)
                                         aaTTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCa                                                                                                    >  1:95294/1‑141 (MQ=255)
                                            tAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAAtt                                                                                                 <  1:576312/141‑1 (MQ=255)
                                             aaaGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCAAGAGTTGCCGAGGTATACGGATAAATATCAAtga                                                                                                >  1:58480/1‑139 (MQ=17)
                                               aGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAAc                                                                                              <  1:243887/141‑1 (MQ=255)
                                                gATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCAGAGTTATTCGGCTAAATATCAATTAAca                                                                                             >  2:188898/1‑140 (MQ=255)
                                                gATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAAAGGTGCCGAATTATTCGGCTAAATATCAATTAACt                                                                                             >  2:660524/1‑141 (MQ=255)
                                                      aaaaTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatgat                                                                                       <  1:693279/141‑1 (MQ=255)
                                                           aaaaaaaGAAATCGAANACTTTATCGCATTTGTTCAAAATGGCGACGTTAAAAATTTGAAAAATTATAAAGACGGATAGATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATta                                                                                  <  1:609917/141‑1 (MQ=14)
                                                           aaaaaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATta                                                                                  <  1:22848/141‑1 (MQ=255)
                                                           aaaaaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATta                                                                                  >  1:48648/1‑141 (MQ=255)
                                                              aaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATaa                                                                               <  1:322096/141‑1 (MQ=255)
                                                              aaaaGAAATAGAACACTGTAAATCCTTTGTTCAATATGGCGACTTGAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCATAGTTGCCGATTTATTCGGCTAAATATCAATTAACTACTCACGCTTATaa                                                                               <  2:286603/141‑1 (MQ=17)
                                                                    aaTCTAAAATTTTAAGTTCTTTATTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGCCTAAATATCAGTTAACTAATGATGATTATAATGTaaa                                                                         <  2:640541/141‑1 (MQ=17)
                                                                    aaTCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaaa                                                                         <  2:349136/141‑1 (MQ=255)
                                                                       cGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACa                                                                      >  1:236833/1‑141 (MQ=255)
                                                                         aaaaCTTTAAGTGCTTTTTTCAATATGGCGATTTTAAAAATTTGAAAAATTATAAATACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAAt                                                                    <  2:101115/141‑1 (MQ=255)
                                                                          aaaCTTTAAGTTCTTTGTTCAATTTGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGATTTATTCGGCTAAATATCAATTGACTGATGATGATTATAATGTAAAACAAtt                                                                   <  2:319114/141‑1 (MQ=255)
                                                                           actcttAAGTTGTTTGTTCAATGTGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTa                                                                  <  2:719858/137‑1 (MQ=255)
                                                                            aCTTTAATTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAGATTATAAAGACGGCGATATTTCATATAATCCAGAGGTCCCTAGTTAGACTGCAAAAGATCATGTAACTAATGATGATTATAATGTAAAAAAAGTaa                                                                 >  2:540045/1‑140 (MQ=17)
                                                                            aCTTTAATTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTAc                                                                 <  2:131767/141‑1 (MQ=255)
                                                                            aCTTTAATTTCTTTGTTCAATATGGCGACTGTAAAAATTTGAAAAATTATAAAGTCGGAGATATTTCATATAATCCAGAGGTGCCGAGTTTTTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAACACAATTAc                                                                 <  2:564997/141‑1 (MQ=17)
                                                                              tttAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGc                                                               <  1:656237/141‑1 (MQ=255)
                                                                                 aaGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCaaa                                                            <  1:588755/141‑1 (MQ=255)
                                                                                  aGTTCTTTGTTCAATATGGCGACGTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAAAAAATACGGaaaa                                                           >  2:217234/1‑141 (MQ=255)
                                                                                       tttGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGAGATTTCATATAATCCAGAGGTGCCGAGTTGTTCGGCTAAATATCAAGTAACTAATGGTGATAATAATGTAGAACAATTACGCAAAAgatat                                                      >  1:295439/1‑141 (MQ=18)
                                                                                            tCAATAAGGAGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgatatgatat                                                 <  2:54954/141‑1 (MQ=255)
                                                                                             cAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGAtata                                                <  1:390977/141‑1 (MQ=255)
                                                                                                   ggCGACATCAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACcgacg                                          <  2:577793/141‑1 (MQ=255)
                                                                                                        cTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTaa                                     <  1:566117/141‑1 (MQ=255)
                                                                                                           tAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGCGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCGAAAGATATGAGATACCGACGAGGAAAgg                                  >  1:445652/1‑140 (MQ=255)
                                                                                                           tAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGCTATACCGACGAGTAAAGc                                  >  2:310038/1‑141 (MQ=255)
                                                                                                           tAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGc                                  >  1:94850/1‑141 (MQ=255)
                                                                                                           tAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACAGACGGGTAAAgt                                  >  2:99781/1‑140 (MQ=255)
                                                                                                            aaagatttaatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCt                                 <  1:559759/129‑1 (MQ=11)
                                                                                                             aagatttaatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTc                                >  1:239402/12‑141 (MQ=11)
                                                                                                              agatttaatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTcc                               >  2:346286/11‑141 (MQ=11)
                                                                                                              agatttaatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTcc                               <  1:665315/131‑1 (MQ=14)
                                                                                                              aaaTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGACAGGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTcc                               <  1:213404/141‑1 (MQ=255)
                                                                                                              aaaTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGCGTAGCTTGATGCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTcc                               <  1:213415/141‑1 (MQ=21)
                                                                                                                 tttGAAAAATTATCAAGACGGATATATGTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTATTGATGATTATAATGTAAAACAATTACGCAAAAGATATTATATACCGACGAGTAAAGCTCCaaa                            <  2:135044/141‑1 (MQ=18)
                                                                                                                 tttGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCaaa                            <  2:607597/141‑1 (MQ=255)
                                                                                                                 ttggAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCaaa                            <  1:227012/138‑1 (MQ=255)
                                                                                                                  ttGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAg                           >  1:706356/1‑141 (MQ=255)
                                                                                                                      aaaaTTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTAt                       <  1:129626/141‑1 (MQ=255)
                                                                                                                       aaaGTATAAAGACGGGGATATTTCATATAAGCCAGAGGTGCCGAGTTATTCGGCTAAATATGAATTAACTAATGATGATTATAATGTAAGAAAATTACGCAAAGGATATGAATTACCGGCGAATAAAGCTCCAAAGtggtt                      >  2:70813/1‑137 (MQ=14)
                                                                                                                         aTTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTAGAATGTAAAACAATTACGCAAAAGATATGTTATACCGACGAGTAAAGCTCCAAAGTTATTGt                    <  2:579368/141‑1 (MQ=255)
                                                                                                                             taAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaaa                >  1:350676/1‑141 (MQ=255)
                                                                                                                               aaGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGATAAAAg              >  2:328355/1‑141 (MQ=255)
                                                                                                                                aGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACTCAAAAGATATGCTATACGTACACGTAAAGCTCCAAAGTTATAGTTAAAAgg             >  2:191006/1‑141 (MQ=17)
                                                                                                                                 gatggAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGt            >  1:547018/4‑141 (MQ=255)
                                                                                                                                   cGGAGCTATTTCACATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTc          <  2:393545/141‑1 (MQ=255)
                                                                                                                                          atTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTCAGGGAAt   >  1:140685/1‑141 (MQ=255)
                                                                                                                                          atTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTCAGGGAAt   <  2:270672/141‑1 (MQ=255)
                                                                                                                                          atTCCATATAATCCAGAGGTGCTGAATTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTCAGGGAAt   <  2:395923/141‑1 (MQ=255)
                                                                                                                                           tttCATCTAATCCAGACGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTCAGGGAAtt  <  2:688115/141‑1 (MQ=255)
                                                                                                                                           tttCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTCAGGGAAtt  >  2:318760/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                           
CCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTCAGGGAATT  >  CP000730/110520‑110799

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: