Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,857,867 C→T 7.6% L169L (TTG→TTA)  USA300HOU_1728 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,857,8670CT 7.6% 191.5 / 2.1 79L169L (TTG→TTA) USA300HOU_1728hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (34/38);  new base T (2/5);  total (36/43)
Fisher's exact test for biased strand distribution p-value = 4.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTATCCGCTTCTGCTTGTGCTTCTCTTTTAGCTTCTTGAATCTCTTGTG  >  CP000730/1857728‑1858006
                                                                                                                                           |                                                                                                                                            
ttttttttACCGCTGCTTGTTGTGCTTTCAATGCCACTGTTTTTGGTTCTTCATTTTTTATAGCTGCACTTTTATCTTTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCa                                                                                                                                             <  2:378800/137‑1 (MQ=17)
cttcttTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTTTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCa                                                                                                                                             <  2:63017/141‑1 (MQ=255)
cttcttTTATCGCTGCTTGTTGTGCTATCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCAGCTTGAGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGTCTCTTGTGATGTATCACTCa                                                                                                                                             <  1:468061/141‑1 (MQ=255)
  tcttTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCaaa                                                                                                                                           <  1:630128/141‑1 (MQ=255)
       tATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAattatt                                                                                                                                      >  1:435498/1‑141 (MQ=255)
       tATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCGTATGATGTttcattcaggttagt                                                                                                                                      >  2:178234/1‑134 (MQ=17)
             tgctTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACt                                                                                                                                <  1:146107/141‑1 (MQ=255)
               tttgttgTGCCTTCAATGCCCCTGCTTTTGGTTCTTCATTTGATTCTGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTCAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTg                                                                                                                              <  2:492310/140‑1 (MQ=25)
                ttgttgTGCTTTCACTGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGCATCTCTTGTGCCCCTTGTGATGTATCTCTCACattcttccccctcgc                                                                                                                             >  2:709246/1‑132 (MQ=37)
                 tgttgtGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCTTCTTGTGATGTATCACTCAAATTATTTGCACTTGct                                                                                                                            >  2:295273/1‑141 (MQ=255)
                      tgCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGcttcttc                                                                                                                       <  1:370140/141‑1 (MQ=255)
                        ttttCAGTGCCACTGTTTTTGGTTTTGCATTTGTTTGTGCTGCGCTTTTGTCTTTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGcttcttc‑tt                                                                                                                    <  2:137864/140‑1 (MQ=17)
                           tCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTTCTTTTTC‑TTTTa                                                                                                                 >  2:523532/1‑141 (MQ=255)
                           tCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTC‑ttttt                                                                                                                 >  2:313379/1‑140 (MQ=255)
                           tCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTC‑TTTTa                                                                                                                 >  1:376700/1‑141 (MQ=255)
                            cAATGCCGCTGCTTTTGGTTATTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACATGCTTCTTC‑TTTTAt                                                                                                                >  1:480277/1‑141 (MQ=255)
                            cAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTC‑TTTTAt                                                                                                                >  1:424260/1‑141 (MQ=255)
                              aTGCCACTGCTTTTGGTTCTTCATTTGCTACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAACTTATTTGCACTTGCTTCTTC‑TTTTATCg                                                                                                              <  1:301045/141‑1 (MQ=255)
                              aTGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTC‑TTTTATCg                                                                                                              >  1:55661/1‑141 (MQ=255)
                                  cACTGCTTTTGGTTCTTCATTTGATATGACTTCTCGTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTC‑TTTTATCgctgc                                                                                                          >  1:372262/1‑141 (MQ=17)
                                      tcTTTTGCTTCTTCATCTTTTGTTGATGCATTTTTATCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCttgt                                                                                                      <  2:372807/140‑1 (MQ=14)
                                          ttGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTtgtg                                                                                                  >  1:314932/1‑141 (MQ=255)
                                              ttcttcATTTGGTACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCttt                                                                                              <  2:625659/141‑1 (MQ=255)
                                                        gATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGTTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGATTTTAAttctcct                                                                                    >  2:108757/1‑135 (MQ=17)
                                                        gATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGCATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGcccag                                                                                    >  2:108771/1‑138 (MQ=255)
                                                        gATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACt                                                                                    >  1:236271/1‑141 (MQ=255)
                                                          tgttgatgtgtttttGTCTTTCTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGc                                                                                  <  2:358586/130‑1 (MQ=11)
                                                           aCAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTC‑TTTCATCGCTGCTTGCTGTGCCTTCAATGCCCCtccc                                                                                 >  2:508689/1‑138 (MQ=17)
                                                           aCAGCTGCACTTTTATCTGTCTCTGCTTGTGCTTCTTTTTTAGCATCTTGAATCTCTTGTGCCTCTTGTGATTTATGAATCTACGCACTTGCACTTGCCTCTTC‑TTTCATCGCTATTTGTTGTGCTTTCGATTCCAGTGCt                                                                                 >  2:421899/1‑141 (MQ=14)
                                                             aGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGAttt                                                                               >  1:300029/1‑141 (MQ=255)
                                                              gctgtgcTTTTGTCTCTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGCATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCtttt                                                                              <  1:348173/135‑1 (MQ=14)
                                                              gCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCtttt                                                                              <  1:591257/141‑1 (MQ=255)
                                                              gCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCtttt                                                                              >  1:759692/1‑141 (MQ=255)
                                                              gCTGCACTATTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCtttt                                                                              <  1:348228/141‑1 (MQ=255)
                                                                tgtgcTTTTGTCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTTTTGTATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGa                                                                            <  1:204110/137‑1 (MQ=255)
                                                                 tcatttttGTCTTTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGAt                                                                           <  2:158557/137‑1 (MQ=255)
                                                                      tttATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCTTCTTGTGATGTATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCttgt                                                                                                      <  2:358549/109‑1 (MQ=12)
                                                                      tttATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCttgt                                                                                                      >  1:358549/1‑109 (MQ=255)
                                                                      tttATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTTAATGCCACTTCtttttttttttt                                                                      >  2:692692/1‑133 (MQ=255)
                                                                      tttATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCttt                                                                      <  1:647950/141‑1 (MQ=255)
                                                                        tATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCGTCTTGTGATGTATCACTCAAATTCTTTGCACTTGCTTCTTC‑TTTTGTCGCTGCTTGTTGTGCTTTTAATGCCACTGGTTTTGTttttttct                                                                    >  1:233385/1‑139 (MQ=14)
                                                                         ttCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTAtt                                                                   <  1:708994/140‑1 (MQ=255)
                                                                           ctttttCTGCTTGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTGTGATTCTTTATTTg                                                                 <  1:411408/138‑1 (MQ=255)
                                                                              tttCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCACTGCCACTGCTTTTGATTCTTTATTTGATa                                                              >  1:263472/1‑141 (MQ=255)
                                                                                tCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACa                                                            <  1:450546/141‑1 (MQ=255)
                                                                                tCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTCTATTTGATACa                                                            <  1:633027/141‑1 (MQ=255)
                                                                                 ttGCTTGTGCTTCTTTTTTTGCTTCTTGATTCTCTTGTGCCTCTTTTTATGTATCTCTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCTCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTTATACAg                                                           <  2:253775/140‑1 (MQ=11)
                                                                                  tGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGc                                                          >  1:391263/1‑141 (MQ=255)
                                                                                     ttGTGCTTCTTTTTTAGCTTCTTGAACCTCTTGTGCCTCTTGTGATGTATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCcac                                                       >  1:244983/1‑141 (MQ=21)
                                                                                      tgtgCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGcttcttc                                                                                                                       >  1:257006/1‑77 (MQ=255)
                                                                                      tgtgCTTCTTTTTTAGCATCTTGAATCTCTTGTGCCTCTTGTGATAACTCACTCAAAATATTTGCACTTGCTTCTTC‑TGTTATCGCTGCTTGTTGTGCCTTCATTGCCTCTGCTTTTGATTATTTATTTGATACAGcaccc                                                      >  2:129261/1‑137 (MQ=11)
                                                                                             tttttttAGCTTCTTGATCCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTAt                                               <  2:244983/140‑1 (MQ=255)
                                                                                              tttttgAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTATc                                              <  1:74664/141‑1 (MQ=255)
                                                                                                 tttAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTGCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTATCCGc                                           >  2:614076/1‑141 (MQ=255)
                                                                                                 tttAGCTTCTTGAACCTCTTGTGCCTCTTGTGATGTATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGAt                                                               >  2:254875/1‑121 (MQ=2)
                                                                                                 tttAGCTTCTTGAACCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGAt                                                               <  1:254875/121‑1 (MQ=255)
                                                                                                  ttaatttcttGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTATCCGCt                                          <  2:305099/136‑1 (MQ=255)
                                                                                                  ttAGCTTCTTGACTCTCTTGTGCCTTTTGTGATGTATCACTCTAATTATTTGCACTTGCTTTTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTATCCGCt                                          <  2:300019/141‑1 (MQ=255)
                                                                                                  ttAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTATCCGCt                                          >  1:291891/1‑141 (MQ=255)
                                                                                                    aGCTTCTTGAATCTCTTGTGCCGCTTGGTATGTATCACTCAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTTTTTCTTTTTTTGATGAAGCTCCCCTTTTTTCTGCttt                                        >  2:426951/1‑140 (MQ=14)
                                                                                                          ttGAATCTTTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTTTTCTTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTATCCGCTTCTGCtt                                   <  2:397588/141‑1 (MQ=255)
                                                                                                             aaTCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCCTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTATCCGCTTCTGCTTGTGc                               <  1:105344/141‑1 (MQ=255)
                                                                                                              aTCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTTTTTTTTTATTTTATACAGCCACACTTTTACCTGCTTTTGCTTGTGCt                              >  2:696072/1‑141 (MQ=14)
                                                                                                              aTCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCGTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTATCCGCTTCTGCTTGTGCt                              >  1:304267/1‑141 (MQ=255)
                                                                                                               tctcTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTATCAGCTTCTGCTTGTGCtt                             >  1:416772/1‑141 (MQ=255)
                                                                                                               tctcTTGTGCCTATTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTATCCGCTTCTGCTTGTGCtt                             >  1:542028/1‑141 (MQ=255)
                                                                                                                  ctTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTATCCGCTTCTGCTTGTGCTtctc                          <  1:722176/141‑1 (MQ=255)
                                                                                                                    tgtgCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTATCCGCTTCTGCTTGTGCTTCTCtt                        <  1:237531/141‑1 (MQ=255)
                                                                                                                     ttgCCTCTTGTGATGTATCACTTAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTATCCGCTTCTGCTTGTGCTTCTCttt                       <  2:413491/140‑1 (MQ=255)
                                                                                                                     gtgCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAg                                                           <  1:542573/105‑1 (MQ=255)
                                                                                                                             tgtgATGTATCACTCAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTATCCGCTTCTGCTTGTGCTTCTCTTTTAGcttct               <  1:28670/141‑1 (MQ=255)
                                                                                                                             tgtgATGTATCACTCAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTATCCGCTTCTGCTTGTGCTTCTCTTTTAGcttct               <  1:402917/141‑1 (MQ=255)
                                                                                                                              gtgATGTATCACTCAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTATCCGCTTCTGCTTGTGCTTCTCTTTTAGcttctt              >  1:649935/1‑141 (MQ=255)
                                                                                                                               tgATGTATCACTCAAATTATTTGCGCTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTAGGCACTACTTCTTTTGATTCTTTATTTGATACAGCCACACTTTTATCCGCTTCTGCTTGTGCTTCTCTTTTAGCTTCTTg             <  1:178234/141‑1 (MQ=18)
                                                                                                                                  tGTATCACTCTAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGTTTCTTTATTTGATACAGCCACACTTTTATCCGCTTCTGCTTGTGCTTCTCTTTTAGCTTCTTGAAt          <  2:397684/141‑1 (MQ=255)
                                                                                                                                       cACTCAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATCCACCAACACTTTTAACTAAAGCTACTTGTGCTACTCTTTTAGCTTCTCGAATCTCtt     <  1:561351/141‑1 (MQ=14)
                                                                                                                                       cACTCAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTATCCGCTTCTGCTTGTGCTTCTCTTTTAGCTTCTTGAATCTCtt     <  1:403458/141‑1 (MQ=255)
                                                                                                                                       cACTCAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTATCCGCTTCTGCTTGGGCGGCTCTTTTAGCTTCTTGAATCTCtt     <  1:183771/141‑1 (MQ=255)
                                                                                                                                       cACTCAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGATTTTGATTCTTTATTTGATACATCCACACTTTTATCCGCTTCTTCTTGTGCTTCTCTTTTATCTTCTTTTATCTAtt     >  1:159790/1‑141 (MQ=17)
                                                                                                                                          tCAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTATCCGCTTCTGCTTGTGCTTCTCTTTTAGCTTCTTGAATCTCTtgtg  <  1:604177/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                            
CTTCTTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGGTTCTTCATTTGATACAGCTGCACTTTTATCTGTTTCTGCTTGTGCTTCTTTTTTAGCTTCTTGAATCTCTTGTGCCTCTTGTGATGTATCACTCAAATTATTTGCACTTGCTTCTTC‑TTTTATCGCTGCTTGTTGTGCTTTCAATGCCACTGCTTTTGATTCTTTATTTGATACAGCCACACTTTTATCCGCTTCTGCTTGTGCTTCTCTTTTAGCTTCTTGAATCTCTTGTG  >  CP000730/1857728‑1858006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑