Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 35,913 C→G 23.9% S213S (TCG→TCC USA300HOU_0026 ← IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073035,9130CG23.9% 445.8 / 222.5 321S213S (TCG→TCCUSA300HOU_0026IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base C (101/141);  new base G (37/39);  total (138/183)
Fisher's exact test for biased strand distribution p-value = 2.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.72e-01

Only 100 of 331 total aligned reads displayed.

CTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTC  >  CP000730/35773‑36053
                                                                                                                                            |                                                                                                                                             
               aaataaaatTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGCTc                                                                                                                                >  1:170029/1‑141 (MQ=255)
               aaataaaatTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATc                                                                                                                                >  1:94181/1‑141 (MQ=255)
                   aaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAaga                                                                                                                          <  2:325815/129‑1 (MQ=11)
                    aaatTGTTATCTAATAAAAATAGAAGTCGCTGTGGCAAAGTAACAAAATATAGCTAACCACTAATTTATCATGTCAGTGTCCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAag                                                                                                                           <  2:85341/141‑1 (MQ=14)
                    aaatTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAag                                                                                                                           <  1:669591/141‑1 (MQ=255)
                    aaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAagag                                                                                                                         <  1:741271/130‑1 (MQ=11)
                      atTGTGATCTAATAAAAATAGAAGGGTTTGTTGAAAAGAAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAagag                                                                                                                         <  2:273787/141‑1 (MQ=255)
                         gtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                                                                                                                      <  1:359196/141‑1 (MQ=255)
                         gtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                                                                                                                    >  2:529666/7‑141 (MQ=11)
                         gtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGATAATCACTAATATATAATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                                                                                                                    >  1:108390/7‑141 (MQ=11)
                           gATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAACTCAGTAGATCTAAAGAGACCTg                                                                                                                    >  2:720003/1‑141 (MQ=255)
                            ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                                                                                                 <  1:298128/138‑1 (MQ=11)
                            ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                                                                                                 <  2:54549/138‑1 (MQ=11)
                            ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCGAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                                                                                                 >  1:748206/4‑141 (MQ=11)
                             tCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                                                                                                                  <  1:19658/141‑1 (MQ=255)
                              ttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCAGtt                                                                                                               <  1:551527/140‑1 (MQ=11)
                                 ataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCtt                                                                                                            <  1:581494/141‑1 (MQ=11)
                                                                                              cAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTCAATACATTCAATACCTTTTAAAGTATGCTTTGCCGTATTTATAc                                                >  2:175143/1‑141 (MQ=11)
                                                                                              cAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACCCCCAATACCTTTTAAAGTATTCTTTCCTGTATTGATAc                                                >  2:199347/1‑141 (MQ=11)
                                                                                              cAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATAc                                                <  2:310403/141‑1 (MQ=11)
                                                                                              cAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATAc                                                <  2:698898/141‑1 (MQ=11)
                                                                                                gtgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt                                              >  1:588571/1‑141 (MQ=17)
                                                                                                gtgtTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt                                              <  1:186880/141‑1 (MQ=32)
                                                                                                gggttcctttAACTTGCCAGCATAATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt                                              <  1:412826/133‑1 (MQ=11)
                                                                                                 tgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACttt                                             >  2:351340/1‑141 (MQ=17)
                                                                                                 tggtCGCTTAACTTGCTAGCATGATGCTAATGTCGTGGCATGGCGAAAATCCGTAGATCTTAAGAGACCTGCGGTTCTTTTTATATAGTGCGTAAATACACTCCATACCTTTTAAAGTATTCTTTGCTGTATTGATACttt                                             <  2:127025/138‑1 (MQ=11)
                                                                                                  tgtCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTg                                            <  2:458465/139‑1 (MQ=12)
                                                                                                  gtTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTg                                            >  1:76460/1‑141 (MQ=17)
                                                                                                  gtTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTg                                            >  1:458020/1‑141 (MQ=17)
                                                                                                  gtTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTg                                            <  1:234466/141‑1 (MQ=17)
                                                                                                   ttCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGa                                           <  1:335445/141‑1 (MQ=11)
                                                                                                   ttCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa                                           <  2:687565/141‑1 (MQ=32)
                                                                                                    tcgtttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAt                                          >  1:136512/5‑141 (MQ=12)
                                                                                                    tCGTTTAACTTGCTAGCATGATGCTAATTGCGTGGCATGGGGAAAAACCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGAt                                          <  1:628231/141‑1 (MQ=11)
                                                                                                    tCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGATACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAt                                          <  1:320849/141‑1 (MQ=17)
                                                                                                     tgtttAACTTGCTAGCATGTTGCTAATTTCGTGGCATGGGGATAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATa                                         <  2:746290/138‑1 (MQ=11)
                                                                                                     cgtttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATa                                         >  1:12204/4‑141 (MQ=17)
                                                                                                     cgtttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATa                                         >  1:221795/4‑141 (MQ=17)
                                                                                                     cGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATa                                         >  2:229078/1‑141 (MQ=17)
                                                                                                     cGCTTAACTTGCTAGCATGATACTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGCGCGTAGATACACGCAATACCTTTTAAAGTATTCTTTGCTGTATTGCTACTTTGATa                                         >  1:60962/1‑141 (MQ=11)
                                                                                                     cGCTTAACTTGCTAGCATGAAGCTAATTTCGTGGCATGGCGAAAATGAGTAAGACTGGAGGAACAAGCGGTTCTTTTTATATAGAGAGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATa                                         >  2:717127/1‑141 (MQ=11)
                                                                                                      gtttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATat                                        <  2:582762/139‑2 (MQ=255)
                                                                                                       tttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc                                       <  1:76057/140‑1 (MQ=17)
                                                                                                        ttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATatct                                      >  2:146635/1‑138 (MQ=255)
                                                                                                        ttAACTGGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCt                                      <  1:273362/141‑1 (MQ=17)
                                                                                                         tAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGGATTCTTTGCCGTATTGATACTTTGATATCtt                                     >  1:211393/1‑141 (MQ=11)
                                                                                                         tAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACTCTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCtt                                     <  1:637615/141‑1 (MQ=17)
                                                                                                          aaCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTg                                    >  2:499624/1‑141 (MQ=17)
                                                                                                           aCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGt                                   <  2:671155/141‑1 (MQ=17)
                                                                                                            cTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGCTCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGCATTGATACTTTGATATCTTGtt                                  >  2:657855/1‑140 (MQ=11)
                                                                                                            cTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtc                                  <  2:605688/141‑1 (MQ=11)
                                                                                                            cTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTGTTTATACTTTGATACCTTGtc                                  >  1:66273/1‑141 (MQ=12)
                                                                                                            cTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtc                                  >  2:472534/1‑141 (MQ=17)
                                                                                                            cTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACAAACCATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtc                                  <  1:148808/141‑1 (MQ=12)
                                                                                                            cTTGCTAGCATGATGCTAATGTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtc                                  >  1:464780/1‑141 (MQ=17)
                                                                                                             ttGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtct                                 <  2:510762/141‑1 (MQ=17)
                                                                                                             ttGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtct                                 <  2:439767/141‑1 (MQ=17)
                                                                                                              tGCTAGCATGATTCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtctt                                <  2:401179/141‑1 (MQ=11)
                                                                                                              tGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctt                                <  1:344305/141‑1 (MQ=17)
                                                                                                              tGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctt                                <  1:433529/141‑1 (MQ=17)
                                                                                                              tGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGCTTCTTTTTATATATAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctt                                <  2:149820/141‑1 (MQ=12)
                                                                                                                cTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttc                              <  2:609683/141‑1 (MQ=17)
                                                                                                                cTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTACTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGGTACTTTGATACCTTGtctttc                              >  1:244059/1‑141 (MQ=12)
                                                                                                                 tAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttct                             <  2:77810/141‑1 (MQ=17)
                                                                                                                    cATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTAc                          >  2:379443/1‑141 (MQ=11)
                                                                                                                    cATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAGCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTATTTGCAGTATTCAAACTTTGATACCTTGTCTTTCTTAc                          >  2:246468/1‑141 (MQ=11)
                                                                                                                    cATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTAc                          >  2:30319/1‑141 (MQ=17)
                                                                                                                      tgatgCTAATTTCGTGGCATGGGGAGAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACtt                        >  1:387062/1‑141 (MQ=11)
                                                                                                                      tgatgCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGCCCTACGGTTCTTTTTATATAGAGCGTAAATACATCCACTACCTTTTAAAGTATTCTTTCCCGTATTGATACTTGCATTTCTTGTCTTTCTTCCtt                        >  2:116820/1‑141 (MQ=11)
                                                                                                                      tgatgCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACtt                        >  1:400598/1‑141 (MQ=255)
                                                                                                                       gattgtgaTTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACttt                       <  2:6277/134‑1 (MQ=11)
                                                                                                                        atgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTa                      >  1:351935/1‑141 (MQ=25)
                                                                                                                        atgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTa                      >  2:281050/1‑141 (MQ=25)
                                                                                                                        atgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTa                      >  2:257377/1‑141 (MQ=25)
                                                                                                                        atgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTAGTCTTTCTTACTTTa                      >  2:210125/1‑141 (MQ=21)
                                                                                                                         tgCTAATTTCGTGGCATGGGGAAAATCCGTAGCTCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTaa                     >  1:16526/1‑141 (MQ=11)
                                                                                                                          gCTAATTTCGTGGCATGGGGAAAATCCGTAGATCCGAAGAGACCTACGGTTCTGTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATCTCTTGTCTTTCTTACTTTAat                    >  2:414963/1‑141 (MQ=11)
                                                                                                                           cTAATTTCGTGGCATGGCGAAAAGGCTAACATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAata                   <  2:535534/141‑1 (MQ=11)
                                                                                                                            tAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTCTTCTTTGCCGTATTGATACTTTGATATCTTGTATTTCTTACTTTAatat                  >  1:306942/1‑141 (MQ=11)
                                                                                                                            tAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAACTTGTCTTTCTTACTTTAatat                  >  2:650796/1‑141 (MQ=21)
                                                                                                                            tAATTTCGTGGCATGGCGAAAATCCGTAGATATGAAGCGGTTGGCGGTTATTGTAGTATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAatat                  >  1:181217/1‑141 (MQ=11)
                                                                                                                             aaTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTGTTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATg                 <  2:565983/141‑1 (MQ=255)
                                                                                                                              aTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGa                <  1:255269/141‑1 (MQ=25)
                                                                                                                                 tCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCCTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACgg             <  1:715953/141‑1 (MQ=255)
                                                                                                                                  ggtagCATGGGGAACATCCGTAGATCTGGAGAAAAATACGGTTCTGTTTATATCGCGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTCTCTTACTTTAATATGACGGt            <  1:538047/137‑1 (MQ=14)
                                                                                                                                  cGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACATTTTATAGATTCATGTGCTGTATTGATCCTTTTATACCTTGTCTTTCTTACTTTAATAGGACgtt            <  2:734756/141‑3 (MQ=11)
                                                                                                                                   gTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTg           <  1:733059/141‑1 (MQ=255)
                                                                                                                                   gTGGCATGGCGAAAATGCGGAGATCTGAAGAGACCTGCAGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATACTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTg           >  1:429928/1‑141 (MQ=11)
                                                                                                                                   gTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTGATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGaggttg           >  2:495778/1‑136 (MQ=255)
                                                                                                                                   gTGGCATGGCGAAAATCCGTAGATCCGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTg           >  1:100074/1‑141 (MQ=255)
                                                                                                                                     ggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAt         >  1:486993/1‑141 (MQ=255)
                                                                                                                                     ggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAt         >  1:250474/1‑141 (MQ=38)
                                                                                                                                       cATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCt       >  2:219096/1‑141 (MQ=38)
                                                                                                                                       cATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGATGATCt       <  2:108390/141‑1 (MQ=255)
                                                                                                                                         tGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTg     <  1:521522/141‑1 (MQ=255)
                                                                                                                                         tGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTg     <  1:291779/141‑1 (MQ=255)
                                                                                                                                         tGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTg     <  2:3328/141‑1 (MQ=38)
                                                                                                                                         tGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTg     <  1:648401/141‑1 (MQ=255)
                                                                                                                                          ggCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGc    <  1:706818/141‑1 (MQ=39)
                                                                                                                                            cGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTc  <  1:68455/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                             
CTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTC  >  CP000730/35773‑36053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: