BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,929,873	C→A	60.4%	intergenic (‑956/‑212)	USA300HOU_1794 ← / → USA300HOU_1796	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,929,873	0	C	A	60.4%	124.3 / 208.7	186	intergenic (‑956/‑212)	USA300HOU_1794/USA300HOU_1796	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (37/37); new base A (59/53); total (96/90)
Fisher's exact test for biased strand distribution p-value = 7.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 188 total aligned reads displayed.

CGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAG  >  CP000730/1929733‑1930013

cGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATCTTTGCCGATGATATAATAGGAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑c                                                                                                                                                >  2:273750/1‑141 (MQ=255)

      aaTCTATCAGCTAGAATCACATAACTTATCAGAAGCAAGTGACTGTGCTTGTAACTTCGCTCTGCATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTAttt                                                                                                                                         <  1:365724/141‑1 (MQ=255)

                                tATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGtcatc                                                                                                              <  1:8121/141‑1 (MQ=255)

                                     gAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCCAATCATAGCACCAGTCATCAGTgg                                                                                                         >  2:660837/1‑141 (MQ=255)

                                     atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGc                                                                                                        >  2:288542/15‑141 (MQ=255)

                                     atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGc                                                                                                        >  2:463539/15‑141 (MQ=255)

                                     atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCACCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGc                                                                                                        >  1:508949/15‑141 (MQ=255)

                                      gcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCt                                                                                                       <  1:87935/128‑1 (MQ=255)

                                      aaGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGc                                                                                                        <  1:652195/141‑1 (MQ=255)

                                      aaGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATATCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGtcagcctttga                                                                                                        >  2:19820/1‑133 (MQ=255)

                                       cggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCtg                                                                                                      >  2:400108/13‑141 (MQ=255)

                                       aGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCt                                                                                                       >  2:568180/1‑141 (MQ=255)

                                         gaagtagatatgCTTGTTTATTCACAGATGATATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGATGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                                                                                    <  2:725921/131‑1 (MQ=255)

                                          gagtcgatatgCTTGTATATTCACAGATGGTATCAGAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                                                                                   <  1:245784/132‑1 (MQ=255)

                                          aagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                                                                                   >  1:434977/10‑141 (MQ=255)

                                           agtcgatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑T‑TTGTGAA‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGct                                                                                                  <  2:498252/133‑2 (MQ=255)

                                           agtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGGGCA‑TTACT‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCCCCAGTCATCAGTGGCTGTGct                                                                                                  >  2:74521/9‑140 (MQ=255)

                                             tagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgt                                                                                                <  1:288542/135‑4 (MQ=255)

                                             tagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgt                                                                                                <  1:7058/135‑4 (MQ=255)

                                             tGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCa                                                                                                 >  2:756786/1‑141 (MQ=255)

                                              gACTGCGTTTGTATACTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTTGTCGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAt                                                                                                <  1:1077/141‑1 (MQ=255)

                                              gACCGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATGTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCCTCAGTGGCTGTGCCAt                                                                                                <  2:702695/141‑1 (MQ=255)

                                               gatttgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                                                                                              <  1:48652/137‑6 (MQ=255)

                                               gatatgCTTTTAAATTCACAGATGGTATAATAATAGAGCCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                                                                                              <  1:272892/137‑6 (MQ=255)

                                               gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                                                                                              >  1:257151/5‑136 (MQ=255)

                                               gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACAAGTCATCAGTGGCTGTGctgttg                                                                                              >  1:11691/5‑136 (MQ=255)

                                                 tgtgCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTGC‑GTTCCCATCATAGCACCAGTCATCTGTGGCTGTGCCAtttc                                                                                             >  2:331535/1‑139 (MQ=255)

                                                 tgtgCTTGTATATTTGCCGATGATATAATAGTAAAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGc                                                                                             >  1:350511/1‑141 (MQ=255)

                                                  atgCTTGTATATTCACAGATGGTATCATCATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGt                                                                                           >  1:304451/2‑141 (MQ=255)

                                                   tgCTTGTATATTTGGCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGt                                                                                           <  2:286128/141‑1 (MQ=255)

                                                   tgCTTGGATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGta                                                                                          <  1:212214/141‑2 (MQ=255)

                                                     cTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatt                                                                                        <  1:416547/141‑4 (MQ=255)

                                                      ttGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGtttt                                                                                        <  2:236979/141‑1 (MQ=255)

                                                       tGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattat                                                                                      >  1:762306/1‑139 (MQ=255)

                                                       tGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattat                                                                                      <  2:685505/141‑6 (MQ=255)

                                                        gtatattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTTGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattata                                                                                     >  2:255734/12‑138 (MQ=255)

                                                         tataTTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatac                                                                                    <  1:384396/141‑8 (MQ=255)

                                                             ttcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAt                                                                                >  2:482053/7‑141 (MQ=255)

                                                             ttcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAt                                                                                >  2:172752/7‑141 (MQ=255)

                                                             ttcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATCCTTAt                                                                                >  2:116941/7‑141 (MQ=255)

                                                               cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACTAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg                                                                              >  1:26139/5‑141 (MQ=255)

                                                               cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACTAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg                                                                              >  1:46857/5‑141 (MQ=255)

                                                               cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg                                                                              >  2:672549/5‑141 (MQ=255)

                                                               cacagatggtATAATAATAAAGTCGCCTATCTCTCAGGCGTCACTT‑‑GACGAAGGGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTG‑GCTCACAGCATAGCCCCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAttt                                                                              >  2:229468/5‑140 (MQ=255)

                                                                 cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc                                                                            >  1:493152/3‑141 (MQ=255)

                                                                 cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc                                                                            >  1:692460/3‑141 (MQ=255)

                                                                    atggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtta                                                                         <  1:622819/141‑3 (MQ=255)

                                                                     tggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGGGGCGGTCCTGTTGCGTATTATACTTATTGGCTTAg                                                                        >  2:61134/4‑141 (MQ=255)

                                                                     aggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTTGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag                                                                        <  2:690362/138‑4 (MQ=255)

                                                                      ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGcatagt                                                                       >  1:586731/3‑136 (MQ=255)

                                                                       gtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttcgta                                                                      >  2:210700/2‑136 (MQ=255)

                                                                        tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGGGGAGGTGCATTCC‑T‑T‑TGCTA‑A‑TCATTTTCACATCGCCGGA‑AGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                                                     >  1:177596/1‑140 (MQ=255)

                                                                        tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTggtctagtaa                                                                     >  2:15738/1‑133 (MQ=255)

                                                                        tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                                                     <  1:309208/141‑2 (MQ=255)

                                                                          taataaTGGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaac                                                                   <  2:251347/141‑4 (MQ=255)

                                                                          taataaTAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaac                                                                   <  2:657295/141‑4 (MQ=255)

                                                                          taataaTAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAAc                                                                   >  2:300211/1‑141 (MQ=255)

                                                                           aataatAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACg                                                                  >  2:173057/1‑141 (MQ=255)

                                                                            ataatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                                 <  1:608995/137‑1 (MQ=255)

                                                                              aatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaa                                                               <  1:323271/139‑1 (MQ=255)

                                                                              aatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaa                                                               <  1:702817/139‑1 (MQ=255)

                                                                               gtagAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCaa                                                               <  1:103884/141‑1 (MQ=255)

                                                                               atagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaac                                                              <  1:230485/140‑2 (MQ=255)

                                                                               atagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaac                                                              <  2:371298/140‑2 (MQ=255)

                                                                                 agagTTCCCTATCGCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGCCGCAATa                                                             <  2:515252/141‑1 (MQ=255)

                                                                                 agagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATGCTTATTGGCTTAGTAAACGCaacaa                                                            <  1:98392/141‑4 (MQ=255)

                                                                                 agagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaa                                                            >  1:13729/1‑138 (MQ=255)

                                                                                 agagTCGCCTATCTCTCAGGAGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaa                                                            >  1:466623/1‑138 (MQ=255)

                                                                                  gagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaa                                                           <  2:521906/141‑5 (MQ=255)

                                                                                  gagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaa                                                           >  1:719709/1‑137 (MQ=255)

                                                                                   agTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACa                                                           >  1:132538/1‑141 (MQ=255)

                                                                                    gTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAC‑G‑C‑T‑CTG‑CGTTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAACTAGACGCAATACaa                                                          <  1:402258/141‑1 (MQ=255)

                                                                                    gTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACaa                                                          <  1:193340/141‑1 (MQ=255)

                                                                                    gTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaata                                                         >  1:742323/1‑135 (MQ=255)

                                                                                     tCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataa                                                        >  1:653965/1‑134 (MQ=255)

                                                                                      cGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataat                                                       >  1:645293/1‑133 (MQ=255)

                                                                                       gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                                      >  2:596056/1‑132 (MQ=255)

                                                                                       gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                                      >  2:412835/1‑132 (MQ=255)

                                                                                       gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                                      <  1:317320/141‑10 (MQ=255)

                                                                                        ccTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTGTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATa                                                      >  1:639477/1‑141 (MQ=255)

                                                                                        ccTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatta                                                     >  2:266377/1‑131 (MQ=255)

                                                                                        ccTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatta                                                     >  2:184971/1‑131 (MQ=255)

                                                                                         cTATCTCTCAGGCGTCAATTTAGACGCCGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAg                                                     >  2:529813/1‑141 (MQ=255)

                                                                                         cTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTTAGTAGATGCAATACAAAATAg                                                     >  2:712681/1‑141 (MQ=255)

                                                                                         cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                                                    <  1:627713/141‑12 (MQ=255)

                                                                                           aTCTCCCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTA‑A‑TCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                                  <  1:416478/141‑14 (MQ=255)

                                                                                                tcAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACat                                              <  2:36661/141‑1 (MQ=255)

                                                                                                        cAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGc                                      <  1:668149/141‑1 (MQ=255)

                                                                                                        cAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATACGTGACATATAGCCGc                                      >  1:517510/1‑141 (MQ=255)

                                                                                                         aaTTTAGACGCAGATAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGTCGCa                                     <  1:547667/141‑1 (MQ=255)

                                                                                                            ttAGACGCAGAGGGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCa                                  <  1:146770/141‑1 (MQ=255)

                                                                                                            ttAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCa                                  <  1:742979/141‑1 (MQ=255)

                                                                                                              aGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACGTATAGCCGCACCAAt                                <  1:568180/141‑1 (MQ=255)

                                                                                                                    agagagGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACAGCATAGCAACAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAAt                          >  1:369635/1‑141 (MQ=255)

                                                                                                                     gagagGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAACAGGTGACATATAGCCGCACCAATAAAAATc                         <  2:582040/141‑1 (MQ=255)

                                                                                                                            ggTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCAc                  >  2:321042/1‑141 (MQ=255)

                                                                                                                                    aa‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCa           <  1:723494/141‑1 (MQ=255)

                                                                                                                                    aa‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCa           <  2:676258/141‑1 (MQ=255)

                                                                                                                                    aa‑G‑G‑GGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCa           <  1:233930/141‑1 (MQ=255)

                                                                                                                                                 c‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAg  >  2:255398/1‑141 (MQ=255)

CGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATG‑C‑TTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAG  >  CP000730/1929733‑1930013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`