BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,929,925	T→A	51.0%	intergenic (‑1008/‑160)	USA300HOU_1794 ← / → USA300HOU_1796	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,929,925	0	T	A	51.0%	7.9 / 198.4	138	intergenic (‑1008/‑160)	USA300HOU_1794/USA300HOU_1796	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (35/32); new base A (42/28); total (78/60)
Fisher's exact test for biased strand distribution p-value = 3.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

Only 100 of 145 total aligned reads displayed.

GCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATTATA  >  CP000730/1929785‑1930059

gCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGTCATTGCGtt                                                                                                                                               <  2:690961/141‑1 (MQ=255)

   tGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattat                                                                                                                                           >  1:762306/1‑139 (MQ=255)

    gtatattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTTGCTAATCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattata                                                                                                                                          >  2:255734/12‑138 (MQ=255)

    gTATATTCCCAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattata                                                                                                                                          <  1:414022/141‑4 (MQ=255)

      atattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatact                                                                                                                                        >  2:452646/10‑136 (MQ=255)

       tatgcggcGATGATATAATAGTAGAGCCGCCTATCTCTCAGGTGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCt                                                                                                                                        <  1:94473/134‑1 (MQ=255)

       aattcacagatggtAAAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACtt                                                                                                                                       <  2:357554/133‑1 (MQ=255)

         ttcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAt                                                                                                                                     >  2:482053/7‑141 (MQ=255)

         ttcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAt                                                                                                                                     >  2:172752/7‑141 (MQ=255)

         ttcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAt                                                                                                                                     <  2:641712/135‑1 (MQ=255)

         ttcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑TGCTAATCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATCCTTAt                                                                                                                                     >  2:116941/7‑141 (MQ=255)

          tcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAtt                                                                                                                                    <  1:588051/136‑1 (MQ=255)

          tcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTC‑GTTCACATCATAGCACCGGTCATCAGTGGCGGTGCTGTTGCGTATTATACTTAtt                                                                                                                                    >  1:23374/6‑141 (MQ=255)

           tGCCGATGATATAATAGAAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTC‑GTTCACATCATAGCACGAGGCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTAtt                                                                                                                                    >  1:244006/1‑141 (MQ=255)

           cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg                                                                                                                                   >  1:744835/5‑141 (MQ=255)

           cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTC‑GTTCACATCATAGCACTAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg                                                                                                                                   >  1:26139/5‑141 (MQ=255)

           cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg                                                                                                                                   >  2:672549/5‑141 (MQ=255)

           cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg                                                                                                                                   >  2:254977/5‑141 (MQ=255)

           cacagatggtATAATAATAAAGTCGCCTATCTCTCAGGCGTCACTT‑‑GACGAAGGGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTG‑GCTCACAGCATAGCCCCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAttt                                                                                                                                   >  2:229468/5‑140 (MQ=255)

             ccGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTgg                                                                                                                                  >  1:666752/1‑141 (MQ=255)

             cagatggtATAATAATATAGTGGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTC‑GTTCACATCATAGCACCAGTCAGCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc                                                                                                                                 <  2:183178/139‑1 (MQ=255)

             cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc                                                                                                                                 >  1:493152/3‑141 (MQ=255)

             cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc                                                                                                                                 >  1:692460/3‑141 (MQ=255)

                atggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtta                                                                                                                              <  1:622819/141‑3 (MQ=255)

                 tggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTC‑GTTCACATCATAGCACCAGTCATCAGGGGCGGTCCTGTTGCGTATTATACTTATTGGCTTAg                                                                                                                             >  2:61134/4‑141 (MQ=255)

                 aggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTTGCTAATCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag                                                                                                                             <  2:690362/138‑4 (MQ=255)

                  ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGcatagt                                                                                                                            >  1:586731/3‑136 (MQ=255)

                   gtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGTGAGTTGGGGCA‑TTA‑C‑TTTGCTAATCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttatta                                                                                                                           >  1:720412/2‑136 (MQ=255)

                   gtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttcgta                                                                                                                           >  2:210700/2‑136 (MQ=255)

                    tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGGGGAGGTGCATTCC‑T‑T‑TGCTAATCATTTTCACATCGCCGGA‑AGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                                                                                                          >  1:177596/1‑140 (MQ=255)

                    tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                                                                                                          <  1:309208/141‑2 (MQ=255)

                    tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                                                                                                          <  1:642279/141‑2 (MQ=255)

                    tataATAAGAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                                                                                                          <  2:747866/141‑2 (MQ=255)

                      taataaTGGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaac                                                                                                                        <  2:251347/141‑4 (MQ=255)

                      taataaTAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaac                                                                                                                        <  2:657295/141‑4 (MQ=255)

                      taataaTAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAAc                                                                                                                        >  2:300211/1‑141 (MQ=255)

                       aataatAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACg                                                                                                                       >  1:81441/1‑141 (MQ=255)

                       aataatAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACg                                                                                                                       >  2:173057/1‑141 (MQ=255)

                        ataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                                                                                      <  2:740733/141‑1 (MQ=255)

                        ataCTAGAGTCGCCCACTTCTCACGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑TGCTAATCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                                                                                      <  2:597991/141‑1 (MQ=255)

                         taatagAGTCGCCTATCTCTCGGGCGGCAATT‑‑GACGAAGCGAGAAGGAATAGTTC‑T‑T‑TGCTAATCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa                                                                                                                     <  1:362513/138‑1 (MQ=255)

                          aatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑a                                                                                                                  <  1:702817/139‑1 (MQ=255)

                           gtagAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCa‑‑a                                                                                                                  <  1:103884/141‑1 (MQ=255)

                           atagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑TGCTAATAATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑ac                                                                                                                 >  2:195353/2‑140 (MQ=255)

                             agagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATGCTTATTGGCTTAGTAAACGCa‑‑acaa                                                                                                               <  1:98392/141‑4 (MQ=255)

                             agagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAAAGCa‑‑acaa                                                                                                               >  2:283390/1‑138 (MQ=255)

                             agagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaa                                                                                                               >  1:13729/1‑138 (MQ=255)

                             agagTCGCCTATCTCTCAGGAGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaa                                                                                                               >  1:466623/1‑138 (MQ=255)

                              gagTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATAc                                                                                                               <  2:708963/141‑1 (MQ=255)

                              gagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaa                                                                                                              <  2:521906/141‑5 (MQ=255)

                               agTCGCCTATCTCTCAAGCGTCAATTTAGACGCAGAGAGGAGGTGAA‑TAA‑G‑G‑TGATGCTTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAGTGCGTTTTTTTCTTAGTGGCCAAGTAGACGCA‑‑ATACa                                                                                                              >  1:193572/1‑141 (MQ=255)

                                gTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACaa                                                                                                             <  1:193340/141‑1 (MQ=255)

                                gTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaata                                                                                                            >  1:742323/1‑135 (MQ=255)

                                 tCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataa                                                                                                           >  1:653965/1‑134 (MQ=255)

                                 gcGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACaa‑‑a                                                                                                          <  1:79128/140‑1 (MQ=255)

                                  cGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataat                                                                                                          >  2:149529/1‑133 (MQ=255)

                                  cGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataat                                                                                                          >  1:645293/1‑133 (MQ=255)

                                   gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataatt                                                                                                         <  1:317320/141‑10 (MQ=255)

                                   gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTGTACTTATTGGCTTAGTAAACGCa‑‑acaaataatt                                                                                                         >  2:499373/1‑132 (MQ=255)

                                   gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataatt                                                                                                         <  2:409843/141‑10 (MQ=255)

                                    ccTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATa                                                                                                       >  2:263200/1‑141 (MQ=255)

                                     cTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTTAGTAGATGCA‑‑ATACAA‑‑AATAg                                                                                                      >  2:712681/1‑141 (MQ=255)

                                     cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataattaa                                                                                                       <  1:627713/141‑12 (MQ=255)

                                     cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataattaa                                                                                                       >  1:140733/1‑130 (MQ=255)

                                     cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑G‑T‑TGCTAATCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataattaa                                                                                                       >  1:32603/1‑130 (MQ=255)

                                      tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTCGCGTATTATACTTATTGGCTTAGCAAACGCa‑‑acaaataattaat                                                                                                      <  1:467103/141‑13 (MQ=255)

                                       ttctctcAGGCGTTAATTTTGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTTTTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTCCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGt                                                                                                    <  2:51561/140‑1 (MQ=255)

                                       aTCTCCCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataattaata                                                                                                     <  1:416478/141‑14 (MQ=255)

                                         ctatctggggTCCATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGa                                                                                                  <  2:269466/132‑1 (MQ=255)

                                            tcAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑at                                                                                              <  2:36661/141‑1 (MQ=255)

                                             cAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAccga                                                                                              >  1:322778/1‑138 (MQ=255)

                                             cAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑ata                                                                                             >  1:259031/1‑141 (MQ=255)

                                             cAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑ata                                                                                             <  1:46988/141‑1 (MQ=255)

                                                gCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑ATAT‑Ag                                                                                         >  1:689926/1‑141 (MQ=255)

                                                    cAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑Gc                                                                                    <  1:668149/141‑1 (MQ=255)

                                                     aaTTTAGACGCAGATAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑ATAT‑AGTC‑GCa                                                                                   <  1:547667/141‑1 (MQ=255)

                                                        ttAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCa                                                                                <  1:742979/141‑1 (MQ=255)

                                                        ttAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCa                                                                                <  1:123006/141‑1 (MQ=255)

                                                          aGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑GTAT‑AGCC‑GCACCAAt                                                                              <  1:568180/141‑1 (MQ=255)

                                                          aGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAAt                                                                              >  1:292570/1‑141 (MQ=255)

                                                                agagagGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAAt                                                                        >  1:503593/1‑141 (MQ=255)

                                                                agagagGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTC‑GTTCACAGCATAGCAACAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAAt                                                                        >  1:369635/1‑141 (MQ=255)

                                                                 gagagGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AACAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATc                                                                       <  2:582040/141‑1 (MQ=255)

                                                                        ggTGTA‑TAA‑G‑G‑TGATGCTTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGGTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCAc                                                                >  1:511171/1‑141 (MQ=255)

                                                                        ggTGTA‑TAA‑G‑G‑TGATGCTTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCAc                                                                >  2:321042/1‑141 (MQ=255)

                                                                                aa‑G‑G‑TGATGCTTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCa                                                         <  1:723494/141‑1 (MQ=255)

                                                                                aa‑G‑G‑GGATGCTTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCa                                                         <  1:233930/141‑1 (MQ=255)

                                                                                              tATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAggg                                              <  1:756786/141‑1 (MQ=255)

                                                                                               aTTTTC‑GTCCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAgggg                                             <  1:165511/141‑1 (MQ=255)

                                                                                               aTTTTC‑GATCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTATTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAgggg                                             >  1:484195/1‑141 (MQ=255)

                                                                                                ttttC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGa                                            <  1:273750/141‑1 (MQ=255)

                                                                                                ttttC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGa                                            >  1:281437/1‑141 (MQ=255)

                                                                                                                  gCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCAACAAATAATTAATAGGCGACTTTATAAGCCAGCATCGA‑AAAAATCCCCACACTACTGC‑AATAGTGTGGGGATTGGTGcattaa                                >  2:23121/1‑138 (MQ=255)

                                                                                                                   cACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATa                          >  2:432630/1‑141 (MQ=255)

                                                                                                                    aCCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATAc                         >  1:699499/1‑141 (MQ=255)

                                                                                                                             caGTGGCTGTGTCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATAATTATTTTCg                <  2:260684/141‑1 (MQ=255)

                                                                                                                                tGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCACATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTg             >  1:121964/1‑141 (MQ=255)

                                                                                                                                 ggCTGTGCCATTGCGTTTTTTTCTTATTGGCGAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGt            <  2:578995/141‑1 (MQ=255)

                                                                                                                                        ccATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AGACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTAGGGGGATTGGTGTATAAGTAAAGACTTATTATAGTTGTCTTAAtt     >  2:498752/1‑141 (MQ=255)

                                                                                                                                         cATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATta    <  2:389378/141‑1 (MQ=255)

GCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTC‑GTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATTATA  >  CP000730/1929785‑1930059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`