Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,680,184 T→A 6.3% intergenic (+236/+296) USA300HOU_2536 → / ← USA300HOU_2537 possible secretory antigen/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,680,1840TA 6.3% 184.9 / 2.4 63intergenic (+236/+296)USA300HOU_2536/USA300HOU_2537possible secretory antigen/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (30/29);  new base A (2/2);  total (32/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.71e-01

AATTGCCGTAATTGAACGTATATTTCTTCTTCAACTATTATTTCATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATTGATGACTCAAAACCTTTATCCCCTTTTCACTCGGTTTAATTGCTTTAATATTTAACACAGTCTCA  >  CP000730/2680044‑2680324
                                                                                                                                             |                                                                                                                                              
aaTTGCCGTAATTGAACGTATATTTCTTCTACAACTATTATTTCATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATATGTTTTCGATATAAAACTTTTTTTAAAtct                                                                                                                                                >  1:183472/1‑141 (MQ=255)
 aTTGCCGTAATTGAACGTATATTTCTTCTTCAACTATTATTTCATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAATTTAACATACGTTTTCGATATAAACCTTGTTCTAActctt                                                                                                                                               >  2:457899/1‑140 (MQ=255)
   tGCCGTAATTGAACGTATATTTCTTCTTCAACTATTATTTCATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCaa                                                                                                                                            >  1:443802/1‑141 (MQ=255)
       gTAATTGAACGTATATTTCTTCTTCAACTATTATTTCATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACATTGTTCTAAATCTCaataat                                                                                                                                        >  2:489994/1‑141 (MQ=255)
               aCGTATATTTCTTCTTCAACTATTATTTCATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCt                                                                                                                               <  2:746177/141‑1 (MQ=255)
               aCGTATATTTCTTCTTCAACTATTATTTCATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCt                                                                                                                               <  2:232470/141‑1 (MQ=255)
                cGTATATTTCTTCTTCAACTATTATTTCATATTTAGTATAGTATCTTTGTTATCACGTTCATCAGTGTGGAAATAATTCGCTATCCTCTGTCATACTTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTg                                                                                                                              <  2:350155/141‑1 (MQ=255)
                 gTATATTTCTTCTTCAACTATTATTTCATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGt                                                                                                                             <  1:461613/141‑1 (MQ=255)
                 gTATATTTCTTCTTCAACTATTATTTCATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGt                                                                                                                             <  1:470687/141‑1 (MQ=255)
                         cttcttcAACTATTATTTCATCTTTAGCATAATCTATATATAAAATTTTCTGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCg                                                                                                                     <  1:213309/141‑1 (MQ=255)
                         cttcttcAACTATTAGTTCATCTTTAGCATAATCTATATATAAAATTTTCTGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCg                                                                                                                     <  1:213139/141‑1 (MQ=255)
                           tcttcAACTATTATTTCATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCACTAATTTTTTGCTGTTTTCATCGTc                                                                                                                   >  2:760860/1‑141 (MQ=255)
                            cttcAACTATTATTTCATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCa                                                                                                                  <  2:131742/141‑1 (MQ=255)
                            cttcAACTATTATTTCATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCa                                                                                                                  <  2:671814/141‑1 (MQ=255)
                             ttcAACTATTATTTCATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAGTAATTCGCTATAACTTAAAATACGTTTTCGATATAAACCTTGTTCTAAATATCAATAATTTTTTGCTGTTTTGATTGTCAt                                                                                                                 >  1:254246/1‑141 (MQ=255)
                               cAACTATTATTTCATCTTTAGCATAATCTATATGTAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGttatta                                                                                                               <  1:521445/141‑1 (MQ=255)
                                 aCTATTATTTCATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGt                                                                                                             >  1:714437/1‑141 (MQ=255)
                                     ttattTCATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCTTCGTTATTAGTTaaa                                                                                                         >  2:115200/1‑141 (MQ=255)
                                           catcttcggCATAGTCTAAGTGTAAAATTTTATGCTTTTGTTTAAGCAATCCACCATAACTTAACATACGTTTTCGATACAAACCTTTTTCTAAATCACTTACAATTTCTTGATTTTTTTCGTCGTCATCAGTTaaaaagt                                                                                                     >  2:277158/9‑139 (MQ=255)
                                           catcttcgacATAGTCTAAGTATAAAATTTTATGTTTTTGTTTAAGCAATCCACCATAACTTAACATACGTTTTCGATACGAACCTTTTTCTAAATCACTTACAATTTCTTGATTTTTTTCATCATCATCCGTTaaaaaat                                                                                                     >  1:397679/10‑140 (MQ=255)
                                           cATCTTCGGCATCGTCTAAGTTTAAAATTTTATGCTTTTGTTTAAGCAATCCACCATAACTTAACATACGTTTTCGATACAAACCTTTTTCTAAATCACTTACAATTTCTTGATTTTTTTCGTCGTCATCAGTTaaaaagt                                                                                                     <  1:468003/141‑3 (MQ=255)
                                            atcgtcggCATAGTCTAAGTTTAAAATTTTATGCTTTTGTTTAAGCAATCCACCATAACTTAACATACGTTTTCGATACAAACCTTTTTCTAAATCACTTACAATTTTTTGATTTTTTTCGTCGTCATCAGTTaaaaagtc                                                                                                    <  1:219701/134‑4 (MQ=255)
                                                 tAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTTTAAATCTCAATAATTTTTTGCTGTTTTTATCGTCATTAGTTAAAAAAATAATTTat                                                                                             >  2:564485/1‑140 (MQ=255)
                                                   gCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACt                                                                                           >  2:546254/1‑141 (MQ=255)
                                                   gCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTAGATATAAACCTTGTTATAAATCTCACTAATTTTTTGCTGTTTTCATCGGCATTAGTTAAAAAAATAATTTCACt                                                                                           >  2:149684/1‑141 (MQ=255)
                                                     aTAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATACGTTCACATGCGTTTTTGATATAAACCGTGTTCTAAATCTCAAAAATTTTTTTCTGTTTTCATCGTCCTTAGTTATAAAAATAATTTAATTgg                                                                                         >  1:368957/1‑140 (MQ=255)
                                                     aTAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGa                                                                                         <  1:188527/141‑1 (MQ=255)
                                                        aTCTATATATAAAATTTTATGCTATTATTTAAATAATTCACTATAATTTAAACTCCATTTTCGATATAAACCTTGTTCTAAATCTCAATAATCTTTTGCTGTTTTTATCTTCATTAGTTAAAAAAATCAtttcaatgtctt                                                                                      >  2:446534/1‑132 (MQ=255)
                                                         tCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTTAATAACTATTTTCTGGTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGttt                                                                                     <  2:304389/141‑1 (MQ=255)
                                                            ttatataAATTTTTATGCTATTATTTAATTAATTCGCTATAACTTAACATACTTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTg                                                                                  <  2:59289/140‑1 (MQ=255)
                                                            atatataAAATTTTATGGTGTTATTTGAGTAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTg                                                                                  >  1:185125/1‑141 (MQ=255)
                                                            atatataAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATCCGTTTTCGATATAAACCTTGTTCTAAATCTCCATCAACTTTTGCTGCGTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTg                                                                                  <  2:735278/141‑1 (MQ=255)
                                                            atatataAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTTGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGttttta                                                                                  >  2:141951/1‑140 (MQ=255)
                                                             tatataAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTga                                                                                 <  2:687252/141‑1 (MQ=255)
                                                             tatataAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTga                                                                                 <  1:624711/141‑1 (MQ=255)
                                                              atataAAATTTTTGGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTgag                                                                                <  2:291856/141‑1 (MQ=255)
                                                                 ataAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGAc                                                                              <  1:647447/141‑1 (MQ=255)
                                                                  taAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGTGAcc                                                                             >  1:294142/1‑141 (MQ=255)
                                                                        tttATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTa                                                                       <  1:251338/141‑1 (MQ=255)
                                                                           aTGCTATTATTTAAATAATTCGCTATAACTTAACCTAGGCCGATGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAAt                                                                    >  1:181540/1‑141 (MQ=255)
                                                                           aTGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAATTGATTTTTTgtttactgttttaat                                                                    >  2:306007/1‑127 (MQ=255)
                                                                           aTGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAAt                                                                    >  2:131039/1‑141 (MQ=255)
                                                                           aTGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAAt                                                                    >  2:251906/1‑141 (MQ=255)
                                                                               tattatTTAAATAATTAGCTACAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTGAATtgat                                                                >  1:395127/1‑141 (MQ=255)
                                                                               tattagTTAAATAATTGGCTCTAACTTAACAAGCGTTTGCGATAGAAGCCTGATTCTAAATCTAAATAATTGTTGGTTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTTAGTTTTTGAGACCTGTTTAAATtgat                                                                <  2:169744/141‑1 (MQ=255)
                                                                                 ttattTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAACAtgatga                                                              >  1:109170/1‑141 (MQ=255)
                                                                                   attTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATTGATGACt                                                            <  1:99653/141‑1 (MQ=255)
                                                                                    tttAAACAATTCGTTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATTGATGACTc                                                           <  2:147816/141‑1 (MQ=255)
                                                                                     ttAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAAACTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCTGTATTAATTAAAAAAATAATTTAACTGTGTTTTTAAGACCGTTTTAAATTCATACCTCa                                                          >  2:153173/1‑141 (MQ=255)
                                                                                          taatTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATTGATGACTCAAAAcc                                                     <  1:91065/141‑1 (MQ=255)
                                                                                            atTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATTGATGACTCAAAACCtt                                                   <  1:183043/141‑1 (MQ=255)
                                                                                                gCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAAATGACTTTTTGAGACCTGTTTAAATTGATGACTCAAAACCTTTATc                                               >  1:712763/1‑141 (MQ=255)
                                                                                                     aaCTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATTGATGACTCAAAACCTTTATCACCtt                                          >  1:115436/1‑141 (MQ=255)
                                                                                                       cTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATTGATGACTCAAAACCTTTATCCCCtttt                                        >  1:435315/1‑141 (MQ=255)
                                                                                                                cGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATTGATGACTCAAAACCTTTATCCCCTTTTCACTCGGtt                               >  1:71813/1‑141 (MQ=255)
                                                                                                                      cGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATTGATGACTCAAAACCTTTATCCCCTTTTCACTCGGTTTAATTg                         >  1:189898/1‑141 (MQ=255)
                                                                                                                      cGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATTGATGACTCAAAACCTTTATCCCCTTTTCACTCGGTTTAATTg                         >  2:24154/1‑141 (MQ=255)
                                                                                                                        atatAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATTGATGACTCAAAACCTTTATCCCCTTTTCCCTCGGTTTAATTGCt                       <  2:303610/141‑1 (MQ=255)
                                                                                                                        atatAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATTGATGACTCAAAACCTTTATCCCCTTTTCACTCGGTTTAATTGCt                       <  1:278381/141‑1 (MQ=255)
                                                                                                                         tataAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAAATGAGTTTTTGAGACCTGTTTAAATTGATGACTCAAAACCTTTATACCCTTTTCACTCGGTTTAATTGCtt                      >  2:323951/1‑141 (MQ=255)
                                                                                                                          ttaAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATTGATGACTCAAAACCTTTATCCCCTTTTCACTCGGTTTAATTGCttt                     <  2:443802/140‑1 (MQ=255)
                                                                                                                               ccTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATACTTTAACTGAGTTTTTGAGACCTGTTTAAATTGATGACTCAAAACCTTTATCCCCTTTTCACTCGGTTTAATTGCTTTActat                >  2:302848/1‑141 (MQ=255)
                                                                                                                                 ttGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAGTTTAACTGAGTTTTTGAGACCTGTTTAAATTGATGACTCAAAACCTTTATCCCCTTTTCACTCGGTTTAATTGCTTTAATAttt              <  2:447300/141‑1 (MQ=255)
                                                                                                                                         aaTCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATTGATGACTCAAAACCTTTATCCCCTTTTCACTCGGTTTAATTGCTTTAATATTTAACACAGt      >  1:98388/1‑141 (MQ=255)
                                                                                                                                          aTCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATTGATGACTCAAAACCTTTATCCCCTTTTCACTCGGTTTAATTGCTTTAATATTTAACACAGtc     <  1:760860/141‑1 (MQ=255)
                                                                                                                                             tcAATACTTTTTTGCTGCTTTCCTCGTCATTAGATAAAAAAATAATTTAACTGAGCTTTCGAGACCTGTTTAAATTGATGACACAAAACCTTTATCCCCTTTTCACTCGGTTTCATTGCTTTCATATTTACCACAGtctcc  >  2:151964/1‑140 (MQ=255)
                                                                                                                                             |                                                                                                                                              
AATTGCCGTAATTGAACGTATATTTCTTCTTCAACTATTATTTCATCTTTAGCATAATCTATATATAAAATTTTATGCTATTATTTAAATAATTCGCTATAACTTAACATACGTTTTCGATATAAACCTTGTTCTAAATCTCAATAATTTTTTGCTGTTTTCATCGTCATTAGTTAAAAAAATAATTTAACTGAGTTTTTGAGACCTGTTTAAATTGATGACTCAAAACCTTTATCCCCTTTTCACTCGGTTTAATTGCTTTAATATTTAACACAGTCTCA  >  CP000730/2680044‑2680324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: