Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 110,660 T→A 12.3% I173I (ATT→ATA)  USA300HOU_0109 → staphylococcal tandem lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730110,6600TA12.3% 175.1 / 18.3 65I173I (ATT→ATA) USA300HOU_0109staphylococcal tandem lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (23/34);  new base A (4/4);  total (27/38)
Fisher's exact test for biased strand distribution p-value = 7.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.31e-01

GTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTCAGGG  >  CP000730/110523‑110795
                                                                                                                                          |                                                                                                                                       
gTTAAAATAGTCAATCATAAAATCTTTCCAAAAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCa                                                                                                                                      <  1:534373/141‑1 (MQ=17)
  tAAAATGGTTGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCata                                                                                                                                    <  1:496149/141‑1 (MQ=255)
      aTGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAAt                                                                                                                                >  2:113798/1‑141 (MQ=255)
          gagATAATAAAATCATGCGAAGAAAAGAAATGAAAAAGGAAACAATAAAAAAAGCAAGCGAAAACTTTAAGTTCTTTGTTCAATATTGTGCTTTAAAAAAAAAAAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCag                                                                                                                            <  2:142188/139‑1 (MQ=12)
                 aaaaaTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGcc                                                                                                                     <  1:309856/140‑1 (MQ=255)
                  aaaaTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCAACTTTAAAAATTTGAAAAATTATAAAGACGGAGAAAG‑TTCAGATACTCCAGAGGTGCCg                                                                                                                    >  2:439133/1‑141 (MQ=17)
                   aaaTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGa                                                                                                                   >  1:283775/1‑141 (MQ=255)
                    aaTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGCGATAT‑TTCATATAATCAAGAGATGCCggg                                                                                                                  >  1:95785/1‑139 (MQ=255)
                       cATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATCTGTCGACTGTAAAAATTGGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGttt                                                                                                               >  1:479013/1‑140 (MQ=255)
                            caacaaAAGAAATTAAAAATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCgg                                                                                                          <  2:479106/141‑1 (MQ=255)
                             aacaaAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGc                                                                                                         >  1:41082/1‑141 (MQ=255)
                              acaaAAGAAATTAAATATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCt                                                                                                        >  1:454266/1‑141 (MQ=255)
                              acaaAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCt                                                                                                        >  1:78316/1‑141 (MQ=255)
                                   aGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAGCTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGTCGAGTTATTCGGCTAAata                                                                                                   <  1:398044/141‑1 (MQ=255)
                                    aaaaTTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAatat                                                                                                  <  2:272906/140‑1 (MQ=255)
                                         tAAAGATGAACAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGACGAGTTATTCGGCTAAATATCAAtt                                                                                             >  2:309365/1‑141 (MQ=255)
                                         tAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAAtt                                                                                             <  1:113798/141‑1 (MQ=255)
                                         tAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAAtt                                                                                             <  1:490725/141‑1 (MQ=255)
                                         tAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTAAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAACTATCAAtt                                                                                             >  1:303120/1‑141 (MQ=255)
                                          aaaGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTa                                                                                            <  1:228055/141‑1 (MQ=255)
                                           aaGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTaa                                                                                           >  1:384402/1‑141 (MQ=255)
                                            aGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAAc                                                                                          <  1:92792/141‑1 (MQ=255)
                                                   aaaaTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTTTTCCATATGACGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatgat                                                                                   <  1:46714/141‑1 (MQ=255)
                                                   aaaaTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatgat                                                                                   <  1:524829/141‑1 (MQ=255)
                                                   aaaaTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatgat                                                                                   >  2:585723/1‑141 (MQ=255)
                                                        aaaaaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATta                                                                              <  2:443500/141‑1 (MQ=255)
                                                             aaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATg                                                                         <  1:61833/141‑1 (MQ=255)
                                                             aaGAAATCGAAAACTTTAAGTTCTTTGTGCAATATGGAGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATg                                                                         <  2:92104/141‑1 (MQ=255)
                                                                aaaTCGAAAACTTTAAGTTCTTTTATCAATATTGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaa                                                                      <  2:313325/141‑1 (MQ=255)
                                                                aaaTCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaa                                                                      >  2:541842/1‑141 (MQ=255)
                                                                   tCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTATAGAGCAATTAACTAATGATGATTATAATGTAAAAc                                                                   >  1:470542/1‑141 (MQ=255)
                                                                    cGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACa                                                                  >  1:65140/1‑141 (MQ=255)
                                                                     gAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACaa                                                                 >  2:222445/1‑141 (MQ=255)
                                                                      aaaaCTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCCGCTACATATCAATTAACCAATGATGATTATAATGTAAAACAAt                                                                <  2:510141/141‑1 (MQ=255)
                                                                           tttAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTACAGTCGAGGATAATGTAAAACAATTACGc                                                           <  1:449843/141‑1 (MQ=18)
                                                                           tttAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTAAGc                                                           >  2:142833/1‑141 (MQ=255)
                                                                           tttAAGTTCTTGGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATCCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGc                                                           <  1:419455/141‑1 (MQ=255)
                                                                               aGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCaaaa                                                       >  2:196730/1‑141 (MQ=255)
                                                                                 ttCCTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAga                                                     <  1:420929/141‑1 (MQ=255)
                                                                                        ttCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgatatgata                                              <  2:65140/141‑1 (MQ=255)
                                                                                               tGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAC‑TTCACATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACcgac                                       <  1:167462/141‑1 (MQ=255)
                                                                                                 gCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACcgacgt                                     >  2:608870/1‑140 (MQ=255)
                                                                                                    aCTTTAAAAATTTGGAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTa                                  <  2:41082/141‑1 (MQ=255)
                                                                                                    aCTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTa                                  >  1:282567/1‑141 (MQ=255)
                                                                                                     cTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTaa                                 <  2:513622/141‑1 (MQ=255)
                                                                                                         aaagatttaatgaaTTATAAAGATGGAGATAT‑ATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCt                             <  2:40809/129‑1 (MQ=11)
                                                                                                         aaagatttaatgaaTTATAAAGATGGAGATAT‑ATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCt                             >  1:117424/13‑141 (MQ=11)
                                                                                                           agatttaatgaaTTATAAAGATGGAGATAT‑ATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAGATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTcc                           <  2:311713/131‑1 (MQ=11)
                                                                                                           agatttaatgaaTTATAAAGATGGAGATAT‑ATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTcc                           >  1:357838/11‑141 (MQ=14)
                                                                                                           aaaTTTGAAAAATTATAAAGACGGAGATAT‑TTCATATACTCCAGGGCTGCCGAGTTATTCGGCTAAATATCCAATAACTAATGATGATTATAATGTAAAACACTTACGCAAAAGATATGATATACCGACGAGTAAAGCTcc                           <  2:290132/141‑1 (MQ=18)
                                                                                                           aaaTGTGAAAAATTACAACGGCAGGGAAGT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGTTATGATATACCGACGAGTAAAGCTcc                           <  2:302818/141‑1 (MQ=17)
                                                                                                            aattaagcaagttATAATGACGG‑GATGTATTTATAGAATCCAGAGGGGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACTAGTAAAGCTCCa                          <  2:303120/130‑1 (MQ=17)
                                                                                                                tGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGt                      <  1:499647/141‑1 (MQ=255)
                                                                                                                 aatgaaTTATAAAGATGGAGATAT‑ATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGtt                     <  1:278007/137‑1 (MQ=255)
                                                                                                                   aaaaTTATAAAGACGGAGATAT‑TTCATCTGATGCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTAt                   >  1:295997/1‑141 (MQ=255)
                                                                                                                   aaaaTTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTAt                   >  1:9535/1‑141 (MQ=255)
                                                                                                                         ataAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaa             <  1:514114/141‑1 (MQ=255)
                                                                                                                         ataAAGACGGAGATAA‑TTCATATAAACCAGAGGCGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaa             <  1:584664/141‑1 (MQ=255)
                                                                                                                          aaaaGATGCAGATAT‑ATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaaa            <  1:259898/140‑1 (MQ=255)
                                                                                                                              gatggAGATAT‑ATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGt        >  1:352719/4‑141 (MQ=255)
                                                                                                                              gatggAGATAT‑ATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGATATTGTTAAAAGGt        >  2:95267/4‑141 (MQ=255)
                                                                                                                              gACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGt        >  1:264865/1‑141 (MQ=255)
                                                                                                                                    gaTAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTCAggg  <  1:104383/141‑1 (MQ=255)
                                                                                                                                    gaTAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTCAggg  <  1:550360/141‑1 (MQ=255)
                                                                                                                                    gaTAT‑TTCATATAACCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTCAggg  <  1:281357/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                       
GTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTCAGGG  >  CP000730/110523‑110795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑