Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 36,087 A→G 8.5% C155C (TGT→TGC)  USA300HOU_0026 ← IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073036,0870AG 8.5% 172.0 / 8.6 71C155C (TGT→TGC) USA300HOU_0026IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base A (36/28);  new base G (2/4);  total (38/33)
Fisher's exact test for biased strand distribution p-value = 4.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.08e-02

ATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAG‑GTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGAT  >  CP000730/35952‑36223
                                                                                                                                        |                                                                                                                                        
ttataGAGCGTAAATACATTCAATACCTTTTAAAGTATTTTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAG‑GTTATTCAGATATTTCGATGTACAATGACAGTc                                                                                                                                     <  2:606110/140‑1 (MQ=255)
atataGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAG‑GTTATTCAGATATTTCGATGTACAATGACAGTc                                                                                                                                     <  1:299872/141‑1 (MQ=255)
  ataGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAG‑GTTATTCAGATATTTCGATGTACAATGACAGTCAg                                                                                                                                   <  1:171427/141‑1 (MQ=255)
    agagCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCCATAAG‑GTTCTTCAGATATTTCGCTGTACAATGACGTTCAgat                                                                                                                                 >  1:185831/1‑139 (MQ=14)
    agagCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTCTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAG‑GTTATTCAGATATTTCGATGTACAATGACAGTCAGgt                                                                                                                                 >  1:541692/1‑141 (MQ=255)
       gCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAG‑GTTATTCAGATATTTCGATGTACAATGACAGTCAGgttta                                                                                                                              <  2:277316/141‑1 (MQ=40)
         gTAAATACATTCAATACCTTTTAAAGTATTCTTTTCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAG‑GTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaag                                                                                                                            <  1:200335/141‑1 (MQ=255)
         gTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAG‑GTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaag                                                                                                                            <  1:334490/141‑1 (MQ=255)
          tAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAG‑GTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagt                                                                                                                           <  1:563098/141‑1 (MQ=255)
            aaTACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACATTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAG‑GTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagttt                                                                                                                         <  1:438594/141‑1 (MQ=255)
             aTACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTTAATAAG‑GTTATTCATATATTTCGATGTACAATGACAGTCAGgttttagttta                                                                                                                        >  1:39995/1‑141 (MQ=255)
             aTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAG‑GTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagttta                                                                                                                        >  1:559015/1‑141 (MQ=255)
              tACATTCACTACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACATTGTCTTTCTTACTTTAATATGACGGTGATCTTGCGCAATGAG‑GTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagtttaa                                                                                                                       >  2:450680/1‑141 (MQ=255)
                 aTTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCACTGAG‑GTTATTCACATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAg                                                                                                                    <  1:418808/141‑1 (MQ=255)
                  ctCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAG‑GTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTGTAAGTTTAAAAGc                                                                                                                   >  2:174166/2‑141 (MQ=255)
                      aTACCTGTTAAAGGATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAG‑GTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTa                                                                                                               >  2:232474/1‑141 (MQ=255)
                          cTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAG‑GTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAAttt                                                                                                           >  1:435338/1‑140 (MQ=255)
                          cTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAG‑GTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTa                                                                                                           >  1:246764/1‑141 (MQ=40)
                           ttttAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAG‑GTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTAc                                                                                                          >  2:104044/1‑141 (MQ=255)
                                 aGTATTCTTTGCTGTATTGATACTTTGATACCCTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAG‑GTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGc                                                                                                    >  2:353433/1‑141 (MQ=255)
                                  gTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAG‑GTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAAcc                                                                                                   >  2:28464/1‑141 (MQ=255)
                                   tATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAG‑GTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCa                                                                                                  >  2:542336/1‑141 (MQ=255)
                                    aTTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAG‑GTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCAt                                                                                                 >  2:421508/1‑141 (MQ=255)
                                        tttGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAG‑GTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCt                                                                                             >  2:191249/1‑141 (MQ=255)
                                           gCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAG‑GTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTAcc                                                                                          <  1:414274/141‑1 (MQ=40)
                                           gCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAG‑GTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTAcc                                                                                          >  2:230587/1‑141 (MQ=255)
                                           gCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAG‑GTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTAcc                                                                                          >  2:230602/1‑141 (MQ=255)
                                            cTGTATTGATACTTTGATACCTTGTCTTTCTTACTATAATCTTGTTGTCATCTTGCTCAATGAG‑GTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCt                                                                                         <  2:277288/141‑1 (MQ=11)
                                             tGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAG‑GTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCtt                                                                                        <  1:135486/141‑1 (MQ=255)
                                              gTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAG‑GTTATTCAGATCTTTGGTGGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTc                                                                                       >  1:291331/1‑141 (MQ=14)
                                              gTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAG‑GTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAACTTTAATTACTTTAGCCATTGCTACCTTc                                                                                       >  2:486422/1‑141 (MQ=255)
                                                  tGATACTTTGGTACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAG‑GTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTg                                                                                   <  2:484166/141‑1 (MQ=255)
                                                     tACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAG‑GTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAATAGCTTTAATTACTTTAGCCATTGCTTCGTTTGtttgtt                                                                                >  2:123951/1‑137 (MQ=14)
                                                     tACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAG‑GTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGagg                                                                                >  1:278887/1‑139 (MQ=255)
                                                      aCTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAG‑GTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACATTCGTTGattt                                                                               >  1:164021/1‑138 (MQ=255)
                                                            aTACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAG‑GTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTg                                                                         <  1:168126/141‑1 (MQ=38)
                                                            aTACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAG‑GTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTg                                                                         >  2:545111/1‑141 (MQ=39)
                                                              aCCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAG‑GTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGAt                                                                       >  1:491180/1‑141 (MQ=38)
                                                              aCCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAG‑GTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGAt                                                                       >  2:574560/1‑141 (MQ=39)
                                                                 ttGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAG‑GTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTg                                                                    >  1:265363/1‑141 (MQ=39)
                                                                 ttGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAG‑GTTATTCAGATATTTCGATGTACAATGACAGACAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTCAAGTTGACTGATCtt                                                                    >  2:455784/1‑140 (MQ=255)
                                                                        tcttACTTTAATATGACGGTGATCTTGCTCAATGAG‑GTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTTCTACCTTCGTTGAAGGTGCCTGATCTGTAATTAc                                                             >  1:60829/1‑141 (MQ=37)
                                                                        tcttACTTTAATATGACGGTGATCTTGCTCAATGAG‑GTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTAc                                                             >  1:391854/1‑141 (MQ=38)
                                                                           tACTTTAATATGGCGGTGATCTTGAT‑AATGTGCGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCtt                                                          <  2:276517/141‑1 (MQ=11)
                                                                               ttAATATGACGGTGATCTTGCTCAATGAG‑GTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGa                                                      <  2:545265/141‑1 (MQ=34)
                                                                                tAATATGACGGTGATCTTGCTCAATGAG‑GTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAg                                                     <  2:265363/141‑1 (MQ=35)
                                                                                   tatGACGGTGATCTTGCTCAATGAG‑GTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGtt                                                  >  1:205022/1‑141 (MQ=35)
                                                                                     tGACGGTGATCTTGCTCAATGAG‑GTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTa                                                >  2:355394/1‑141 (MQ=34)
                                                                                     tGACGGTGATCTTGATCAATGAG‑GTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTACCTACCTTTTGAGGCTTa                                                <  1:104425/141‑1 (MQ=11)
                                                                                      gACGGTGATCTTGCTCAATGAG‑GTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAc                                               <  2:199603/141‑1 (MQ=25)
                                                                                      gACGGTGATCTTGCTCAATGAG‑GTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAc                                               <  1:389180/141‑1 (MQ=34)
                                                                                       aCGGTGATCTTGCTCAATGAG‑GTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAcc                                              <  2:458278/141‑1 (MQ=35)
                                                                                         ggTGATCTTGCTCAATGAG‑GTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCaa                                            <  1:191249/141‑1 (MQ=32)
                                                                                         ggTGATCTTGCTCAATGAG‑GTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCaa                                            <  1:142439/141‑1 (MQ=33)
                                                                                         ggTGATCTTGCTCAATGAG‑GTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAAATGCCTTTTGAGGTTTACCaa                                            <  2:588408/141‑1 (MQ=18)
                                                                                         ggTGATCTTGCTCAATGAG‑GTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGAGCCTGCTCTGTAATTACCTTTTGAGGTTTACCaa                                            >  2:38464/1‑141 (MQ=17)
                                                                                         ggTGATCTTGCTCAACGAG‑GGTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGCGGTTTACCaa                                            >  2:377296/1‑141 (MQ=16)
                                                                                          gTGATCCTGCTCAATGAG‑GTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCaaa                                           <  1:546345/141‑1 (MQ=17)
                                                                                              tCTTGCTCAATGAG‑GTTATTCTGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGt                                       <  2:461082/141‑1 (MQ=17)
                                                                                                ttGCTCGACGAG‑GTTATTCAGATACTGCGACGTACAATGACTGCCAGCGCTAAGTTTAAAGGCTTTAACTCCAATAACCATGTCTACCTTCGCCAAAGAGGCCTGATCTGTAATTACCTTTTTAGGTTTACCAAATTAttt                                     >  2:171576/1‑141 (MQ=2)
                                                                                                 tGCTCAATGAG‑GTTATTCAGATATCTCGCATTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCGTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCaattcggtta                                    <  2:276536/141‑9 (MQ=11)
                                                                                                         gAG‑GTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATCGTTACCCTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACg                            <  1:353433/141‑1 (MQ=12)
                                                                                                          aG‑GTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGt                           >  1:24372/1‑141 (MQ=32)
                                                                                                             tttATTCTGATTTTTCGATGTATATTGTCGGTCTGGTTTGCGTTTAACAGCCTTGGCTTTGGTGTCCATTGCTACCTTCGTTGAAGTGACTTCATCTACGGTTACCTTTTGAGGTTTACCAACTTGTTTAATGAGACGttt                         <  2:278887/140‑1 (MQ=2)
                                                                                                             gTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTACCTACCTTTTGAGGCTTACCAAAGTGTTTAATGAGACGttt                         <  1:542336/141‑1 (MQ=11)
                                                                                                                         tttCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCata             >  2:312544/1‑141 (MQ=32)
                                                                                                                            cGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGc          >  1:585779/1‑141 (MQ=32)
                                                                                                                             gATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGTTAAACGCATATGCt         >  1:312879/1‑141 (MQ=17)
                                                                                                                                    aaggACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCCTTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGAt  <  1:246785/138‑1 (MQ=2)
                                                                                                                                    aaTGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGAt  <  1:548517/141‑1 (MQ=2)
                                                                                                                                    aaTGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGAt  <  1:104044/141‑1 (MQ=32)
                                                                                                                                        |                                                                                                                                        
ATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAG‑GTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGAT  >  CP000730/35952‑36223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑