Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,454,626 A→T 12.0% L48L (CTA→CTT USA300HOU_2315 → hypothetical membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,454,6260AT12.0% 192.5 / 15.8 73L48L (CTA→CTTUSA300HOU_2315hypothetical membrane protein
Reads supporting (aligned to +/- strand):  ref base A (35/29);  new base T (4/5);  total (39/34)
Fisher's exact test for biased strand distribution p-value = 7.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.84e-01

AGAACAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCTTTAATTTTACCTTTAATTATTCTAAT  >  CP000730/2454487‑2454766
                                                                                                                                           |                                                                                                                                            
agaaCAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTATATAATTGTTGTCAAACACTTTATATTTGAAGTTACGGATCTAg                                                                                                                                             >  2:472832/1‑141 (MQ=255)
      aTAAATTTTCTGGTTTTCAAGGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCAt                                                                                                                                       <  2:289036/141‑1 (MQ=255)
       tAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCAtt                                                                                                                                      <  2:66418/141‑1 (MQ=255)
                 ggTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCa                                                                                                                            <  1:351556/141‑1 (MQ=255)
                  gTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCaa                                                                                                                           >  2:422207/1‑141 (MQ=255)
                  gTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCaa                                                                                                                           <  2:236612/141‑1 (MQ=255)
                      tCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACGGCTCTAGAACCATTAATGGCTCAAATctt                                                                                                                       >  2:491785/1‑139 (MQ=255)
                         aTGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTg                                                                                                                    <  1:562867/141‑1 (MQ=255)
                          tGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGTTGTCAAACAATTTATATTTGAAGTTACAGATCTTGCACCATTATTTGCTGCAATCATTTGt                                                                                                                   >  1:232495/1‑141 (MQ=255)
                           gggCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGATATCCATTATGCTCAGAGATTTACAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTGATTGCTGCAATCATTTGta                                                                                                                  >  2:162100/1‑141 (MQ=255)
                           gggCAATGACCATTCTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTGta                                                                                                                  <  1:12380/141‑1 (MQ=255)
                            ggCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTGtat                                                                                                                 >  1:234643/1‑141 (MQ=255)
                             gCATTGACGATTTTCGTCTTCTTTGTCATTACAATGGAGTTATCCATTAAGCCCAGAGATTTCCAGTCTATAATTGGTATCAACCACTTTATATTTGAAGTTACAGATCTACCACCATTAATTGCTGAAATCATTTCtatc                                                                                                                >  2:226400/1‑140 (MQ=255)
                             gCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTGtata                                                                                                                >  2:467433/1‑141 (MQ=255)
                              ccgtGACGCTTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGGGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCAGTTGTATAc                                                                                                               <  2:388510/138‑1 (MQ=255)
                               aaagACGATTTTCATCTTCTATGAAAACACAATGACATAATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTGATATTTGAAGTTACAGACCTAGCACCATTAATTGCTGCAATCATTTGTATACt                                                                                                              <  1:250263/138‑1 (MQ=255)
                                aTGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTGAGAGATTACCATACTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCCATCATTTGTATACTc                                                                                                             >  2:34483/1‑141 (MQ=255)
                                aTGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTGTATACTc                                                                                                             <  1:270302/141‑1 (MQ=255)
                                aTGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTGTATACTc                                                                                                             <  1:467433/141‑1 (MQ=255)
                                  gACGTTTTTCGTTTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTGTATACTCGt                                                                                                           <  2:49157/141‑1 (MQ=255)
                                           cGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAata                                                                                                  >  2:480742/1‑141 (MQ=255)
                                                tcttTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATaaaa                                                                                             >  1:180128/1‑141 (MQ=255)
                                                tcttTGTCATTACAATGGCATTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGCGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATaaaa                                                                                             >  1:367487/1‑141 (MQ=255)
                                                     gTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTc                                                                                        >  1:411666/1‑141 (MQ=255)
                                                           aCAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACtt                                                                                  >  1:603443/1‑141 (MQ=255)
                                                              aTGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCa                                                                               <  1:493211/141‑1 (MQ=255)
                                                              aTGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCa                                                                               <  1:493254/141‑1 (MQ=255)
                                                               tGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAg                                                                              >  1:402530/1‑141 (MQ=255)
                                                                ggCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGTTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAgg                                                                             <  2:377422/141‑1 (MQ=255)
                                                                ggCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAgg                                                                             <  2:377494/141‑1 (MQ=255)
                                                                 gcattATCCATTATGCTTTGAGTATTCCAGTCTATAATTGGTGTCGAACACTTTATATTTGAAGTTACAACTCTAGTACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGt                                                                            <  1:491785/138‑1 (MQ=255)
                                                                      aTCCATTATGCGCATAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAACCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTaaa                                                                       <  2:145388/141‑1 (MQ=255)
                                                                       tCCATTATGCTCAGAGTTTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGCAGTGACAGATCTAGCACCATTAATTGCTGCAATCATTTGTATACTCGGTTGCAAATATAAAAAGGACAAACGTGCAGGTTTaaaa                                                                      >  2:536389/1‑141 (MQ=255)
                                                                         cATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAAtt                                                                    <  1:368736/141‑1 (MQ=255)
                                                                               gCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTGTATACTCGTTGTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCCAt                                                              >  2:196150/1‑141 (MQ=255)
                                                                                cTCAGAGATTTCCAGTCTATAAGTCGCGTCAAACACTGTATATTTGTTGTTTCTGGTCTAGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATc                                                             <  1:441820/141‑1 (MQ=255)
                                                                                 tCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCa                                                            >  1:212331/1‑141 (MQ=255)
                                                                                    gagaTTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGGTACAGATCTAGCACAATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAAGATAAAAAGGTCCAACTTGCAGGTTTAAAATTCGCAATCAccc                                                         >  1:485855/1‑141 (MQ=255)
                                                                                         ttCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGaaa                                                    <  2:405866/141‑1 (MQ=255)
                                                                                         ttCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGaaa                                                    >  1:474889/1‑141 (MQ=255)
                                                                                          tCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAg                                                   >  2:605319/1‑141 (MQ=255)
                                                                                          tCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGGTACAGATCGAGCACCATTAATTGCTGCAATCATTTGTATACTCGATTACAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAg                                                   <  2:302429/141‑1 (MQ=255)
                                                                                          tCCAGGCTATAATTGGTGTCAAACACTGTATATTTGAAGTTACAGATCGAGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAAGATAAAAAGGTCCAACTTGCAGGTTTAAAAGTCTCAATCAGCCTGAAAg                                                   <  1:275026/141‑1 (MQ=255)
                                                                                           ccAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGt                                                  >  2:399399/1‑141 (MQ=255)
                                                                                                 tataATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGa                                            >  1:602975/1‑141 (MQ=255)
                                                                                                  ataATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGaa                                           >  2:119501/1‑141 (MQ=255)
                                                                                                  ataATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGaa                                           >  1:563856/1‑141 (MQ=255)
                                                                                                  ataATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGAAATCCTTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGaa                                           >  1:83204/1‑141 (MQ=255)
                                                                                                     atcggTGTCAAACACTTTATATTTGAAGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGt                                        <  1:84493/138‑1 (MQ=255)
                                                                                                          tgtCAAACACTTTATATTTGAAGTTACAGATCTAGCACGATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTAAATCAGCCGGAAAGTAATTGAACGTATAtt                                   >  2:401890/1‑141 (MQ=255)
                                                                                                          tgtCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGCACGTCTAtt                                   >  1:33805/1‑141 (MQ=255)
                                                                                                                  acTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAGAGTAATTGAACGTCTATTGCTAGCtt                           <  2:367487/141‑1 (MQ=255)
                                                                                                                  acTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCtt                           <  2:180128/141‑1 (MQ=255)
                                                                                                                  acTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCtt                           <  2:431937/141‑1 (MQ=255)
                                                                                                                     ttATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCTTTaa                        >  1:504980/1‑141 (MQ=255)
                                                                                                                      tataTTTGAAGTTCCAGATCTAGCGCCATTGATTGCTGCAATGATTGGTATGCTCGTGGTGAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCTTTAAt                       >  2:509907/1‑141 (MQ=255)
                                                                                                                       atatTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCTTTAAtt                      >  1:553628/1‑141 (MQ=255)
                                                                                                                       atatTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATCTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAATGAATTGAACGTCAATTGCTATCTTTAAtt                      >  1:347024/1‑141 (MQ=255)
                                                                                                                        tctttGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCTTTAAttt                     <  2:328950/139‑1 (MQ=255)
                                                                                                                        tatTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCTTTAAttt                     <  2:234643/141‑1 (MQ=255)
                                                                                                                        tatTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCTTTAAttt                     >  1:185022/1‑141 (MQ=255)
                                                                                                                        tatTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCTTTAAttt                     >  1:511518/1‑141 (MQ=255)
                                                                                                                        tatTTGAAGTTACAGATCTAACACCATTAATTGCCGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCTTTAAttt                     <  2:566377/141‑1 (MQ=255)
                                                                                                                              aaGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCTTTAATTTTACCtt               <  1:593023/141‑1 (MQ=255)
                                                                                                                              aaGTTACAGATCTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTAAGCGTCTATTGCTAGCTTTAATTTTACCtt               >  1:316950/1‑141 (MQ=255)
                                                                                                                                gTTACAGATCTAGCAACAATAGCTCGGAGAATGATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCATGAAACTAATTGAACGACTATTGTGACCTTTAATTTTACTTTTa             >  1:146357/1‑141 (MQ=255)
                                                                                                                                 tttcAGATTTTGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCTTTAATTTTACCTTTaa            <  2:262692/138‑1 (MQ=255)
                                                                                                                                 ttACAGATCTAGTACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCTTTAATTTTACCTTTaa            <  2:262614/141‑1 (MQ=255)
                                                                                                                                   aCAGATCTAGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCTTTAATTTTACCTTTAatt          >  1:11947/1‑141 (MQ=255)
                                                                                                                                   aCAGATCTAGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAAAGTCTATTGCTAGCTTTAATTTTACCTTTAatt          >  1:273186/1‑141 (MQ=255)
                                                                                                                                       aTCTAGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCTTTAATTTTACCTTTAATTATTc      <  1:480742/141‑1 (MQ=255)
                                                                                                                                       aTCTAGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCTTTAATTTTACCTTTAATTATTc      <  1:605319/141‑1 (MQ=255)
                                                                                                                                        tCTAGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTACTAGCTTTAATTTTACCTTTACTTATTCt     >  1:240144/1‑141 (MQ=255)
                                                                                                                                           aGCACCATTAATTGCTGCAATCATTAGTATACTCGTTTTCAAATATAAAAACATCCAACTTGAAGGTTTAAAAATCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTACACTTAATTTCACCTCTCattgctaagtt  >  2:570588/1‑133 (MQ=255)
                                                                                                                                           |                                                                                                                                            
AGAACAATAAAATTTCTGGTTTTCAATGGGCAATGACGATTTTCGTCTTCTTTGTCATTACAATGGCGTTATCCATTATGCTCAGAGATTTCCAGTCTATAATTGGTGTCAAACACTTTATATTTGAAGTTACAGATCTAGCACCATTAATTGCTGCAATCATTTGTATACTCGTTTTCAAATATAAAAAGGTCCAACTTGCAGGTTTAAAATTCTCAATCAGCCTGAAAGTAATTGAACGTCTATTGCTAGCTTTAATTTTACCTTTAATTATTCTAAT  >  CP000730/2454487‑2454766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: