Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 36,378 C→T 12.2% L58L (TTG→TTA)  USA300HOU_0026 ← IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073036,3780CT12.2% 150.6 / 12.0 65L58L (TTG→TTA) USA300HOU_0026IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base C (31/25);  new base T (5/3);  total (36/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.97e-01

CGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCT  >  CP000730/36238‑36504
                                                                                                                                            |                                                                                                                              
cGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATAc                                                                                                                                <  1:312544/141‑1 (MQ=40)
agCAACCAAATATCTAATGTATGTCTCTCTGCATCAATTGCACGATATAAATCGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATAc                                                                                                                                <  1:355394/140‑1 (MQ=255)
  cAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACaa                                                                                                                              <  2:246764/141‑1 (MQ=255)
  cAACCAAATATCTAATGTATGGCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACaa                                                                                                                              <  2:435338/141‑1 (MQ=255)
    aCCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAATCTTTTTTATGGTTTTTCTTCCAAATTTGATACaaaa                                                                                                                            >  1:540058/1‑141 (MQ=255)
    aCCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAACTTTTTTATGCTTTTTCCTCCAAATTTGATATaaaa                                                                                                                            >  1:243191/1‑141 (MQ=255)
      cAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAAtt                                                                                                                          >  2:74738/1‑141 (MQ=255)
          tatCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTgggg                                                                                                                      <  2:232590/141‑1 (MQ=255)
          tatCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGATCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCTTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTTATACAAAATTgggg                                                                                                                      >  2:336857/1‑141 (MQ=255)
           atCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGc                                                                                                                     >  2:174817/1‑141 (MQ=255)
                   tatgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCtt                                                                                                             >  2:486745/1‑141 (MQ=255)
                    ttgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTg                                                                                                            <  1:61055/140‑1 (MQ=255)
                      gtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGac                                                                                                          >  2:332576/1‑140 (MQ=255)
                       tCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAAc                                                                                                         >  2:182778/1‑141 (MQ=21)
                       tCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAACTGGGGGCATCTTGtttcac                                                                                                         >  1:380277/1‑137 (MQ=14)
                       tCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGTGGCATATTCTTGAAc                                                                                                         >  1:142263/1‑141 (MQ=255)
                       tCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAAc                                                                                                         <  1:123951/141‑1 (MQ=40)
                         ccTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAAccc                                                                                                       <  1:377296/141‑1 (MQ=40)
                               cATCAATGGTACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATATGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCTTATCCTTGAACCCAACGGt                                                                                                 <  1:455784/141‑1 (MQ=255)
                                   aaTGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCAATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACGAAATTGGGGCATATCCTTGAACCCaaagtgagcc                                                                                             >  2:483970/1‑133 (MQ=14)
                                       gCACGTTATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTCTACCGtt                                                                                         <  1:216200/141‑1 (MQ=255)
                                          cGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTTaa                                                                                      >  1:68961/1‑141 (MQ=255)
                                          cGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGaa                                                                                      >  1:96042/1‑141 (MQ=40)
                                            atatAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAatg                                                                                    <  2:379554/141‑1 (MQ=40)
                                                aattAGCTCCATTTTCCTCTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAAtgatga                                                                                <  1:483970/138‑1 (MQ=255)
                                                 aaTAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGATATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGaa                                                                               >  1:541872/1‑141 (MQ=11)
                                                 aaTACCGCCATTTTCCTTTTATTTTGATGTACGTCGCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATCAGAAAAAACACTGGGGCATATTCTTGAACCCAACGGTAGACCGTTCAATGATGaa                                                                               <  2:585779/141‑1 (MQ=11)
                                                    aGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCACCGGTAGACCGTTGGATGATGAACGt                                                                            >  1:544553/1‑141 (MQ=255)
                                                    aGCTCCATTTTCCTGTGATTTTGATGTACGCCTCATCAATACGCCATTTGTAATAAGCGTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATGGAGGAACATTCTTCAACCCAACCGACAACCCTGGGATGATGAACGt                                                                            >  2:559397/1‑141 (MQ=11)
                                                      cTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGttt                                                                          >  1:392754/1‑141 (MQ=255)
                                                         cATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTaca                                                                       >  1:481481/1‑141 (MQ=40)
                                                          aTTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTTATACAACATTGGGGCATATTCTTGAACCCAACGGTAGACCGCTGCATGGTCACCGtttttac                                                                      >  2:247622/1‑137 (MQ=14)
                                                          aTTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTacac                                                                      >  1:525587/1‑141 (MQ=40)
                                                           ttttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcacc                                                                     <  1:38464/141‑1 (MQ=40)
                                                               ccTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGt                                                                 <  2:96042/141‑1 (MQ=40)
                                                               ccTTTTATTTTGACAAACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGTACGTTTACACCACGt                                                                 <  2:467641/141‑1 (MQ=11)
                                                                 ttttATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTc                                                               <  1:455602/141‑1 (MQ=255)
                                                                  tttATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTcc                                                              >  1:32683/1‑141 (MQ=40)
                                                                   ttATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTccc                                                             <  1:174817/141‑1 (MQ=40)
                                                                   ttATTGTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTccc                                                             <  1:300952/141‑1 (MQ=255)
                                                                     tttttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTTTGGTGTGTCTGCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCtt                                                           <  1:17232/140‑1 (MQ=14)
                                                                     aTTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCtt                                                           <  2:461496/141‑1 (MQ=39)
                                                                          gATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGATTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGGAGACCGTTGAATGATGAACGTTTAAACAACGTGCCCTTGatat                                                      >  1:507434/1‑141 (MQ=14)
                                                                          gATGTACGTCTCATCAATACGCAATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAAATGGGAAATATAATTACACCCAACGGTAACACGTTGAATGATGAACGTTTACACCACGTTCCCTTAatat                                                      >  1:580722/1‑141 (MQ=14)
                                                                             gTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAGATTTGATACAAAATTGGGGAATATTCGTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTACCTTAATATTTc                                                   >  2:336583/1‑141 (MQ=255)
                                                                                 ttctcATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGat                                               <  2:312879/140‑1 (MQ=40)
                                                                                  tctcATCAATACGCCATTTGTAATAAGTTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGata                                              <  2:515355/141‑1 (MQ=255)
                                                                                   ctcATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGatat                                             >  1:251282/1‑141 (MQ=40)
                                                                                       tcaATACGCCATTTGTAATCAAAGATTTGATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAATGGTAGACCGTTGAATGATGAACGTTTACACAACGTTCCCTTAATATTTCAGATATATCa                                         >  2:281828/1‑141 (MQ=11)
                                                                                       tcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCa                                         <  1:572591/141‑1 (MQ=40)
                                                                                          aTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGa                                      <  1:447603/141‑1 (MQ=39)
                                                                                          aTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGa                                      <  1:486745/141‑1 (MQ=39)
                                                                                          aTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAAGTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGa                                      <  2:366754/141‑1 (MQ=255)
                                                                                             cGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGTCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATag                                   >  2:177700/1‑140 (MQ=255)
                                                                                               ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAAGGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACt                                 >  2:84331/1‑141 (MQ=255)
                                                                                                        ataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGtata                        <  1:191739/141‑1 (MQ=40)
                                                                                                           aGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCt                     <  2:408153/141‑1 (MQ=38)
                                                                                                           aGCTTGTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTCCCCTTAATATTTCAGATATATCACGATAACTCAATGTATctct                     >  2:86107/1‑141 (MQ=255)
                                                                                                             cTTTTTTAGGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTa                   <  1:426853/141‑1 (MQ=37)
                                                                                                              ttttttATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAg                  >  1:356141/1‑141 (MQ=35)
                                                                                                               tttttATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGa                 >  2:553933/1‑141 (MQ=35)
                                                                                                                ttttATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACAATAACTCAATGTATATCTTAGaa                >  2:116333/1‑140 (MQ=25)
                                                                                                                       atttttCTTCCACAGTTGAAACCAAACCGGGGCATATTCTTGAACCCAACGGTAGGCCGTTGAATGATGAACGTGTACACCACGTTCCCTTAATGTTTCAGATATATCACCATACCTCAATGTATATCTAAGATAATGGcc         >  2:37945/2‑141 (MQ=11)
                                                                                                                          tttCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCACTGTATATCTTAGATAGTAGCCAAc      >  1:241858/1‑141 (MQ=25)
                                                                                                                            tcttcCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGAAGACCGTTGGATGATGAACGTGTACACCACGTTCCCTTACTATTTCAGATATACCACGCTAACCCCGTGTCTATCTTAGAtagtcaacgtagc    >  1:283545/1‑132 (MQ=11)
                                                                                                                             cttcCAAATTGGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGc   <  1:576262/141‑1 (MQ=25)
                                                                                                                              ttcCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGGTACCTCAATGTATATCTTAGCTAGTAGACAACggat  >  2:499882/1‑139 (MQ=255)
                                                                                                                                            |                                                                                                                              
CGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCT  >  CP000730/36238‑36504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑