Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	110,651	C→T	9.2%	D170D (GAC→GAT)	USA300HOU_0109 →	staphylococcal tandem lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	110,651	0	C	T	9.2%	173.8 / 6.1	65	D170D (GAC→GAT)	USA300HOU_0109	staphylococcal tandem lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (26/33);  new base T (2/4);  total (28/37)
Fisher's exact test for biased strand distribution p-value = 6.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.76e-01

AGATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGT  >  CP000730/110514‑110789                                                                                                                                          |                                                                                                                                            aGATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGga                                                                                                                                          <  2:12120/141‑1 (MQ=255) aGATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTATTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGTCGga                                                                                                                                          >  1:313325/1‑141 (MQ=255)    tatCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAACTTATAAAGACGgagaa                                                                                                                                       >  1:240813/1‑140 (MQ=255)      tCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAATTTCTTTGTTCAATTTGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGatat                                                                                                                                     >  2:278464/1‑141 (MQ=255)      tCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGatat                                                                                                                                     <  1:610582/141‑1 (MQ=255)          gTTAAAATAGTCAATCATAAAATCTTTCCAAAAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCa                                                                                                                                <  1:534373/141‑1 (MQ=17)            tAAAATGGTTGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCata                                                                                                                              <  1:496149/141‑1 (MQ=255)                aTGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAAt                                                                                                                          >  2:113798/1‑141 (MQ=255)                    gagATAATAAAATCATGCGAAGAAAAGAAATGAAAAAGGAAACAATAAAAAAAGCAAGCGAAAACTTTAAGTTCTTTGTTCAATATTGTGCTTTAAAAAAAAAAAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCag                                                                                                                      <  2:142188/139‑1 (MQ=12)                           aaaaaTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGcc                                                                                                               <  1:309856/140‑1 (MQ=255)                            aaaaTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCAACTTTAAAAATTTGAAAAATTATAAAGACGGAGAAAG‑TTCAGATACTCCAGAGGTGCCg                                                                                                              >  2:439133/1‑141 (MQ=17)                             aaaTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGa                                                                                                             >  1:283775/1‑141 (MQ=255)                              aaTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGCGATAT‑TTCATATAATCAAGAGATGCCggg                                                                                                            >  1:95785/1‑139 (MQ=255)                                 cATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATCTGTCGACTGTAAAAATTGGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGttt                                                                                                         >  1:479013/1‑140 (MQ=255)                                      caacaaAAGAAATTAAAAATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCgg                                                                                                    <  2:479106/141‑1 (MQ=255)                                       aacaaAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGc                                                                                                   >  1:41082/1‑141 (MQ=255)                                        acaaAAGAAATTAAATATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCt                                                                                                  >  1:454266/1‑141 (MQ=255)                                        acaaAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCt                                                                                                  >  1:78316/1‑141 (MQ=255)                                             aGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAGCTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGTCGAGTTATTCGGCTAAata                                                                                             <  1:398044/141‑1 (MQ=255)                                              aaaaTTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAatat                                                                                            <  2:272906/140‑1 (MQ=255)                                                   tAAAGATGAACAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGACGAGTTATTCGGCTAAATATCAAtt                                                                                       >  2:309365/1‑141 (MQ=255)                                                   tAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAAtt                                                                                       <  1:113798/141‑1 (MQ=255)                                                   tAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAAtt                                                                                       <  1:490725/141‑1 (MQ=255)                                                   tAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTAAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAACTATCAAtt                                                                                       >  1:303120/1‑141 (MQ=255)                                                    aaaGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTa                                                                                      <  1:228055/141‑1 (MQ=255)                                                     aaGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTaa                                                                                     >  1:384402/1‑141 (MQ=255)                                                      aGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAAc                                                                                    <  1:92792/141‑1 (MQ=255)                                                             aaaaTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTTTTCCATATGACGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatgat                                                                             <  1:46714/141‑1 (MQ=255)                                                             aaaaTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatgat                                                                             <  1:524829/141‑1 (MQ=255)                                                             aaaaTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatgat                                                                             >  2:585723/1‑141 (MQ=255)                                                                  aaaaaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATta                                                                        <  2:443500/141‑1 (MQ=255)                                                                       aaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATg                                                                   <  1:61833/141‑1 (MQ=255)                                                                       aaGAAATCGAAAACTTTAAGTTCTTTGTGCAATATGGAGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATg                                                                   <  2:92104/141‑1 (MQ=255)                                                                          aaaTCGAAAACTTTAAGTTCTTTTATCAATATTGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaa                                                                <  2:313325/141‑1 (MQ=255)                                                                          aaaTCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaa                                                                >  2:541842/1‑141 (MQ=255)                                                                             tCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTATAGAGCAATTAACTAATGATGATTATAATGTAAAAc                                                             >  1:470542/1‑141 (MQ=255)                                                                              cGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACa                                                            >  1:65140/1‑141 (MQ=255)                                                                               gAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACaa                                                           >  2:222445/1‑141 (MQ=255)                                                                                aaaaCTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCCGCTACATATCAATTAACCAATGATGATTATAATGTAAAACAAt                                                          <  2:510141/141‑1 (MQ=255)                                                                                     tttAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTACAGTCGAGGATAATGTAAAACAATTACGc                                                     <  1:449843/141‑1 (MQ=18)                                                                                     tttAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTAAGc                                                     >  2:142833/1‑141 (MQ=255)                                                                                     tttAAGTTCTTGGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATCCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGc                                                     <  1:419455/141‑1 (MQ=255)                                                                                         aGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCaaaa                                                 >  2:196730/1‑141 (MQ=255)                                                                                           ttCCTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAga                                               <  1:420929/141‑1 (MQ=255)                                                                                                  ttCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgatatgata                                        <  2:65140/141‑1 (MQ=255)                                                                                                         tGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAC‑TTCACATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACcgac                                 <  1:167462/141‑1 (MQ=255)                                                                                                           gCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACcgacgt                               >  2:608870/1‑140 (MQ=255)                                                                                                              aCTTTAAAAATTTGGAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTa                            <  2:41082/141‑1 (MQ=255)                                                                                                              aCTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTa                            >  1:282567/1‑141 (MQ=255)                                                                                                               cTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTaa                           <  2:513622/141‑1 (MQ=255)                                                                                                                   aaagatttaatgaaTTATAAAGATGGAGATAT‑ATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCt                       >  1:117424/13‑141 (MQ=11)                                                                                                                   aaagatttaatgaaTTATAAAGATGGAGATAT‑ATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCt                       <  2:40809/129‑1 (MQ=11)                                                                                                                     agatttaatgaaTTATAAAGATGGAGATAT‑ATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAGATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTcc                     <  2:311713/131‑1 (MQ=11)                                                                                                                     agatttaatgaaTTATAAAGATGGAGATAT‑ATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTcc                     >  1:357838/11‑141 (MQ=14)                                                                                                                     aaaTTTGAAAAATTATAAAGACGGAGATAT‑TTCATATACTCCAGGGCTGCCGAGTTATTCGGCTAAATATCCAATAACTAATGATGATTATAATGTAAAACACTTACGCAAAAGATATGATATACCGACGAGTAAAGCTcc                     <  2:290132/141‑1 (MQ=18)                                                                                                                     aaaTGTGAAAAATTACAACGGCAGGGAAGT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGTTATGATATACCGACGAGTAAAGCTcc                     <  2:302818/141‑1 (MQ=17)                                                                                                                      aattaagcaagttATAATGACGG‑GATGTATTTATAGAATCCAGAGGGGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACTAGTAAAGCTCCa                    <  2:303120/130‑1 (MQ=17)                                                                                                                          tGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGt                <  1:499647/141‑1 (MQ=255)                                                                                                                           aatgaaTTATAAAGATGGAGATAT‑ATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGtt               <  1:278007/137‑1 (MQ=255)                                                                                                                             aaaaTTATAAAGACGGAGATAT‑TTCATCTGATGCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTAt             >  1:295997/1‑141 (MQ=255)                                                                                                                             aaaaTTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTAt             >  1:9535/1‑141 (MQ=255)                                                                                                                                   ataAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaa       <  1:514114/141‑1 (MQ=255)                                                                                                                                   ataAAGACGGAGATAA‑TTCATATAAACCAGAGGCGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaa       <  1:584664/141‑1 (MQ=255)                                                                                                                                    aaaaGATGCAGATAT‑ATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaaa      <  1:259898/140‑1 (MQ=255)                                                                                                                                        gACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGt  >  1:264865/1‑141 (MQ=255)                                                                                                                                          |                                                                                                                                            AGATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATAT‑TTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGT  >  CP000730/110514‑110789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑