Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 665,392 T→G 100% L79* (TTA→TGA)  USA300HOU_0608 → possible acetytransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730665,3920TG100.0% 156.0 / ‑6.5 46L79* (TTA→TGA) USA300HOU_0608possible acetytransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (29/16);  minor base C (1/0);  total (30/16)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ACAACAGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGATAATAAAAAT  >  CP000730/665256‑665522
                                                                                                                                        |                                                                                                                                  
acaacaGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGatat                                                                                                                                >  2:89133/1‑141 (MQ=255)
 caacaGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATAtt                                                                                                                               <  1:167506/141‑1 (MQ=255)
 aaacaGTGAATCAATTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGTCTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATAtt                                                                                                                               <  2:232346/140‑1 (MQ=255)
  aacaGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATAttt                                                                                                                              >  1:141761/1‑141 (MQ=255)
   acaGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTACCTTTATTTACGGTGAAGAATGATAtttt                                                                                                                             >  2:36227/1‑140 (MQ=255)
     aGTTAATCACTTGTTAGAAGCTGCATAGAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATc                                                                                                                           >  2:334051/1‑141 (MQ=255)
               ttGTTAGAAGCTTCATATAATCAACAGGATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTAt                                                                                                                 <  2:571260/141‑1 (MQ=255)
                 gTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGt                                                                                                               >  1:393474/1‑141 (MQ=255)
                        gCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCa                                                                                                        <  1:473398/141‑1 (MQ=255)
                        gCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACAACGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCa                                                                                                        >  2:413500/1‑141 (MQ=255)
                          ttCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGa                                                                                                      >  1:94029/1‑141 (MQ=255)
                          ttCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTAGGTTAAACCAGa                                                                                                      >  1:427298/1‑141 (MQ=255)
                                      aCATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGCTGGCTTTGCTAACTTTATTCACGGTGAAGAATCATATTTATCCGCTCATTATGTTAAACCAGAACCGccccctac                                                                                          >  2:56705/1‑134 (MQ=255)
                                        atcatTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAg                                                                                        >  2:352947/1‑141 (MQ=255)
                                         tcatTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAgg                                                                                       >  1:467091/1‑141 (MQ=255)
                                            tttAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTa                                                                                    >  2:80076/1‑141 (MQ=255)
                                                 agaaaagaCTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATggaa                                                                               >  2:242552/1‑139 (MQ=255)
                                                     aagaCTCCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAga                                                                           >  2:519294/1‑140 (MQ=255)
                                                      agaCTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCa                                                                          >  2:361508/1‑141 (MQ=255)
                                                      aaatttCAATAACAATATTTCTTCTTCGTTGACTAAGGTATTCCAAACTTATGCTTTGCTTACTTTATTTACGGTGAAGAATGAAGTATATCACCTCATTATGTTAAACCATAATCGCAACATACAGGTTCTGGTACAGCa                                                                          <  2:283724/137‑1 (MQ=255)
                                                       ctcTTCAAGAACAATTAGTCGTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCGGCTCATTATGTTAAACCAGAATCGCAACATACTGGTTATGGTAAAGCAt                                                                         >  2:378110/3‑141 (MQ=255)
                                                        aCTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCAtt                                                                        >  2:85698/1‑141 (MQ=255)
                                                           tCAAGAAGAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGtt                                                                     <  2:79121/141‑1 (MQ=255)
                                                                   aaTTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTCACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAATCCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAg                                                             >  2:366416/1‑141 (MQ=255)
                                                                     ttattCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGa                                                           <  1:477256/141‑1 (MQ=255)
                                                                          cTTAGTCGTTGAAGCAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGCTATGGTACAGCATTGTTAAATGAAGGATTATc                                                      <  1:408998/141‑1 (MQ=255)
                                                                          cTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATc                                                      <  1:334051/141‑1 (MQ=255)
                                                                                 gTTGAAGAAGGCAATGAAATCGTTGGCTTTGCTAACTTTATTGACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGtttt                                               <  1:373591/141‑1 (MQ=255)
                                                                                      agaagGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGa                                          <  1:126354/141‑1 (MQ=255)
                                                                                             aaTGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAAttt                                   <  2:141761/141‑1 (MQ=255)
                                                                                              ttGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTg                                  <  2:347641/140‑1 (MQ=255)
                                                                                                gACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATATGaa                                >  1:538998/1‑141 (MQ=255)
                                                                                                  cATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATCAATTTGAAgg                              >  2:175542/1‑141 (MQ=255)
                                                                                                  cATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAgg                              <  1:200006/141‑1 (MQ=255)
                                                                                                     cGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATAAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTAACACGTTTTGAAGATAAATTTGAAGgtgt                           >  1:323292/1‑141 (MQ=255)
                                                                                                      gTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGtt                          >  1:96659/1‑141 (MQ=255)
                                                                                                         ggCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTAc                       >  1:22143/1‑141 (MQ=255)
                                                                                                          gCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACt                      <  1:361508/141‑1 (MQ=255)
                                                                                                            tttGCTAACTTTATTTCCGGTGAAGAATGATATTTATCAGCTCATTATGTTGAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTa                    <  1:501645/141‑1 (MQ=255)
                                                                                                                   aCTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAg             >  2:44955/1‑141 (MQ=255)
                                                                                                                         tttaCGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAAGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGataata       >  1:320593/1‑141 (MQ=255)
                                                                                                                          ttaCGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACCGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGAtaataa      >  1:79063/1‑141 (MQ=255)
                                                                                                                          ttaCGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGAtaataa      >  1:476414/1‑141 (MQ=255)
                                                                                                                            aCGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGATAATaaaa    >  2:204711/1‑141 (MQ=255)
                                                                                                                            aCGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGATAATaaaa    >  1:170950/1‑141 (MQ=255)
                                                                                                                              ggTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTCTGGTACAGCATTGTTAAATGAAGGATTATCACGTTTGTAAGATAAATTTAAAGGTGTTTACTTAGAGGTAGATAGTAAAAAt  >  2:290168/1‑141 (MQ=255)
                                                                                                                                        |                                                                                                                                  
ACAACAGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGATAATAAAAAT  >  CP000730/665256‑665522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑