Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 707,910 A→G 100% intergenic (‑1/‑121) USA300HOU_0653 ← / → USA300HOU_0654 ABC superfamily ATP binding cassette transporter, ABC protein/possible metal‑dependent transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730707,9100AG100.0% 157.4 / ‑6.5 48intergenic (‑1/‑121)USA300HOU_0653/USA300HOU_0654ABC superfamily ATP binding cassette transporter, ABC protein/possible metal‑dependent transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (26/21);  minor base C (0/1);  total (26/22)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAA  >  CP000730/707772‑708042
                                                                                                                                          |                                                                                                                                    
cTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGATGCATACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACaaatg                                                                                                                                    >  2:194606/1‑139 (MQ=255)
  tGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGtt                                                                                                                                  <  1:229834/141‑1 (MQ=255)
      aaGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTtatc                                                                                                                              >  2:68392/1‑140 (MQ=255)
        gggAAGATTTACCAGCAGCGTTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATTAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAGTCTTTTGTTTCTAACAAGCGTTTATAcc                                                                                                                            >  1:396448/1‑141 (MQ=255)
           aaGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATAcctcc                                                                                                                         <  1:37504/141‑1 (MQ=255)
                ttACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAAtt                                                                                                                    <  1:307907/141‑1 (MQ=255)
                ttACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAAtt                                                                                                                    >  1:167320/1‑141 (MQ=255)
                  aCCAGCACCTTTCGTGCCCATGATACCAATTATTTCGCCGCGTACTGTTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAACAACAGATTTAAATCTTTTGTTTCTACAAAGCGTTTAGACCTCCTAAGTaa                                                                                                                  <  2:477196/141‑1 (MQ=255)
                   ccAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTaaa                                                                                                                 >  2:108078/1‑141 (MQ=255)
                       cACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGtt                                                                                                             >  1:203561/1‑141 (MQ=255)
                       cACCATTCGGGCCCATGATACCAATTATTTAGCCGCGTAATGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACATAAAAGCACAGGTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGtt                                                                                                             >  1:158961/1‑141 (MQ=255)
                            ttCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGAACTCACCCTTTCGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTAAGGc                                                                                                        >  2:341900/1‑141 (MQ=255)
                                            caatgacttccgctcgTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTATATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGt                                                                                        <  2:256636/127‑1 (MQ=255)
                                                  tttCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTctaata                                                                                  >  2:414605/1‑141 (MQ=255)
                                                          cgTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTgaga                                                                          >  2:518905/1‑141 (MQ=255)
                                                           gTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGaa                                                                         >  2:278838/1‑141 (MQ=255)
                                                             aCTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAata                                                                       <  1:112594/141‑1 (MQ=255)
                                                                  tATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAACGTTTGGTCTAACCTAATTAATTGTATAATAAACTAAGAATATTTAt                                                                  >  1:156702/1‑141 (MQ=255)
                                                                    tCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCa                                                                >  1:335112/1‑141 (MQ=255)
                                                                       aTAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGt                                                             >  1:220532/1‑141 (MQ=255)
                                                                        tAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTc                                                            >  1:181940/1‑141 (MQ=255)
                                                                        tAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTc                                                            <  1:368074/141‑1 (MQ=255)
                                                                          aGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCaa                                                          <  2:396448/141‑1 (MQ=255)
                                                                             aaaTGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTa                                                       >  2:556018/1‑141 (MQ=255)
                                                                             aaaTGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTa                                                       >  1:119864/1‑141 (MQ=255)
                                                                               aTGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTaaa                                                     >  1:492270/1‑141 (MQ=255)
                                                                                tGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAAt                                                    >  1:478607/1‑141 (MQ=255)
                                                                                 gTTTTTAAGTACATGCTTATTACCTAGAGACAGATTTAAATCTTGTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAAGATTTATCATGTCAAGTAAAtt                                                   <  2:258557/141‑1 (MQ=255)
                                                                                    tttAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATAATGTCAAGTAAATTCgg                                                >  2:540710/1‑140 (MQ=255)
                                                                                     ttAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATCTTTATCATGTCAAGTAAATTCGTg                                               >  2:40392/1‑141 (MQ=255)
                                                                                     ttAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTg                                               >  2:192878/1‑141 (MQ=255)
                                                                                     ttAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTg                                               >  2:333100/1‑141 (MQ=255)
                                                                                     ttAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTg                                               >  2:165567/1‑141 (MQ=255)
                                                                                     ttAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTg                                               >  2:165512/1‑141 (MQ=255)
                                                                                            cATGCTTATTACCTAAAAACAGATTTAAGTCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGatataat                                        >  1:362028/1‑141 (MQ=255)
                                                                                                cTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAg                                    <  1:278838/141‑1 (MQ=255)
                                                                                                  tattaCCTAACAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGAc                                  >  2:243276/1‑141 (MQ=255)
                                                                                                  tattaCCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACGGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGAc                                  <  1:111215/141‑1 (MQ=255)
                                                                                                               cAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAgg                     <  1:518905/141‑1 (MQ=255)
                                                                                                               cAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAgg                     <  1:243276/141‑1 (MQ=255)
                                                                                                               cAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAgg                     <  1:535872/141‑1 (MQ=255)
                                                                                                                  aTTCAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGa                  <  1:385550/141‑1 (MQ=255)
                                                                                                                         tCTTTTGTTTCTATCAGGCGTTTATACCTCCTAATTAAAAGTTTAGGCTATCTTAATTAATTGTATAATAAACTGAGATTATTTATGATGTCAAGTAAATTCGTGAGTTAATATAGGCGATGTATGTGAGGTGAAAGTATg           <  2:3570/141‑1 (MQ=255)
                                                                                                                          tttttGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGt          <  2:203557/140‑1 (MQ=255)
                                                                                                                              ttctacTAACAACCATTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTAATATAATATAGACAATGTATGTGAGGTTAAAGTATGTTAAc      <  2:186853/136‑1 (MQ=255)
                                                                                                                              tGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAAc      <  1:241368/141‑1 (MQ=255)
                                                                                                                                tttCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTg    <  2:335112/141‑1 (MQ=255)
                                                                                                                                 ttCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTga   <  1:10708/141‑1 (MQ=255)
                                                                                                                                  tCTAACAAGCGTTTATAGCTCCTAATTAACAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTgaa  <  1:273584/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                    
CTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAA  >  CP000730/707772‑708042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: