| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 861,896 | C→T | 6.6% | N459N (AAC→AAT) | pgm → | bisphosphoglycerate mutase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 861,896 | 0 | C | T | 6.6% | 178.5 / 2.9 | 61 | N459N (AAC→AAT) | pgm | bisphosphoglycerate mutase |
| Reads supporting (aligned to +/- strand): ref base C (29/28); new base T (2/2); total (31/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01 | |||||||||||
GAAGCGGTTGATGAATGTTTAGGAGAAGTGGTTGATAAGATTTTAGACATGGACGGTTATGCAATTATTACTGCTGACCATGGTAACTCTGATCAAGTATTGACGGATGATGATCAACCAATGACTACGCATACAACGAACCCAGTACCAGTGATTGTAACAAAAGAAGGCGTTACACTTAGAGAAACTGGTCGCTTAGGTGACTTAGCACCTACATTATTAGATTTATTAAATGTAGAACAACCTGAAGATATGACAGGTGAATCTTTAATT > CP000730/861756‑862028 | gAAGCGGTTGATGAATGTTTAGGAGAAGTGGTTGATAAGATTTTAGACATGGACGGTTATGCAATTATTACTGCTGACCATGGTAACTCTGATCAAGTATTGACGGATGATGATCAAACCATGACTACGCATACAACGAAc < 1:565895/141‑1 (MQ=255) aaGCGGTTGATGAATGTTTAGGAGAAGTGGTTGATAAGATTTTAGACATGGACGGTTATGCAATTATTACTGCTGACCATGGTAACTCTGATCAAGTATTGACGGATGATGATCAACCAATGACTACGCATACAACGAAcc > 1:541269/1‑141 (MQ=255) cGGTTGATGAATGTTTAGGAGAAGTGGTTGATAAGATTTTAGACATGGACGGTTATGCAATTATTACTGCTGACCATGGTAACTCTGATCAAGTATTGACGGATGATGATCAACCAATGACTACGCATACAACGAATCCAg > 1:404449/1‑141 (MQ=255) cGGTTGATGAATGTTTAGGAGAAGTGGTTGATAAGATTTTAGACATGGACGGTTATGCAATTATTACTGCTGACCATGGTAACTCTGATCAAGTATTGACGGATGATGATCAACCAATGACTACGCATACAACGAATCCAg > 1:404445/1‑141 (MQ=255) cGGTTGATGAATGTTTAGGAGAAGTGGTTGATAAGATTTTAGACATGGACGGTTATGCAATTATTACTGCTGACCATGGTAACTCTGATCAAGTATTGACGGATGATGATCAACCAATGACTACGCATACAACGAACCCAg > 1:166220/1‑141 (MQ=255) gTTGATGAATGTTTAGGAGAAGTGGTTGATAAGATTTTAGACATGGACGGTTATGCAATTATTACTGCTGACCATGGTAACTCTGATCAAGTATTGACGGATGATGATCAACCAATGACTACGCATACAACGAACCCAGTa < 1:511772/141‑1 (MQ=255) atgaATGTTTATGTGAAGCGTTTTATAAGATTTTAGATCTGGACGGTTATGCAATTCTTACTGCTGACCATGGTAACTCTCATCAACTGTTGACGGATGATGATCAACCAATGACTACGCACACAACGAACCCAGTACCAg < 2:250828/141‑1 (MQ=255) ttAGGAGAAGTGGTGGATAAGATTTTAGACATGGACGGTTATCCAATTATTACTGCTCACCATCGTAATGCTGATCAAGTATTGCCGGCTCACGATCAACCAATGACTACGCATCCAACGAACCCAGTACCAGTGATTGTa < 2:143949/141‑1 (MQ=255) ggAGAAGTGGTTGATAAGATTTTAGACATGGACGGTTATGCAATTATTACTGCTGACCATGGTAACTCTGATCAAGTATTGACGGATGATGATCAACCAATGACTACGCATACAACGAACCCAGTACCCGTGATTGTAACa > 1:494630/1‑141 (MQ=255) aaGTGGTTGATAAGATTTTAGACATGGACGGTTATGCAATTATTACTGCTGACCATGGTAACTCTGATCAAGTATTGACGGATGATGATCAACCAATGACTACGCATACAACGAACCCAGTACCAGTGATTGTAACAAaag > 1:462374/1‑141 (MQ=255) tttgTTTATAAGCTTTTAGACATGGACGGTTATGCAATTATTACTGCTGATCATGGTAACTCTGATCAAGTATTGACGGATGATGATCAACCAATGACTACGCATACAACGAACCCAGTACCAGTGATTGTAACAAaagaa < 2:298335/138‑1 (MQ=255) tGGTTGATAAGATTTAAGACATGGACGGTTATGCAATTATTACTGCTGACCATGGTAACTCTGATCAAGTATTGACGGATGATGATCAACCAATGACTACGCATACAACGAACCCAGTACCAGTGATTGTAACAAaagaag < 1:509127/141‑1 (MQ=255) gATAAGATTTTAGACATGGACGGTTATGCAATTATTACTGCTGACCATGGTAACTCTGATCAAGTATTGACGGATGATGATCAACCAATGACTACGCATACAACGAACCCAGTACCAGTGATTGTAACAAAAGAAGGCGtt < 1:361083/141‑1 (MQ=255) aTAAGATTTTAGACATGGACGGTTATGCAATTATTACTGCTGACCATGGTAACTCTGATCAAGTATTGACGGATGATGATCAACCAATGACTACGCATACAACGAACCCCGCACAAGTGATTGTAACAAAAGAAGGCGtaa > 2:130499/1‑139 (MQ=255) ttttAGACATGGACGGTTATGCAATTATTACTGCTGACCATGGTAACTCTGATCAAGTATTGACGGATGATGATCAACCAATGACTACGCATACAACGAACCCAGTACCAGTGATTGTAACAAAAGAAGGGGTTACACATa > 2:68787/1‑141 (MQ=255) ttttAGACATGGACGGTTATGCAATTATTACTGCTGACCATGGTAACTCTGATCAAGTATTGACGGATGATGATCAACCAATGACTACGCATACAACGAACCCAGTACCAGTGATTGTAACAAAAGAAGGCGTTACACTTa < 2:541269/141‑1 (MQ=255) ttttAGACATGGACGGTTATGCAATTATTACTGCTGACCATGGTAACTCTGATCAAGTATTGACGGATGATGATCAACCAATGACTACGCATACAACGAACCCAGTACCAGTGATTGTAACAAAAGAAGGCGTTACACTTa < 1:145372/141‑1 (MQ=255) ttAGACATGGACGGTTATGCAATTATTACTGCTGACCATGGTAACTCTGATCAAGTATTGACGGATGATGATCAACCAATGACTACGCATACAACGAACCCAGTACCAGTGATTGTAACAAAAGAAGGCGTTACACTTaga > 1:411534/1‑141 (MQ=255) tAGACATGGACGGTTATGCAATTATTACTGCTGACCATGGTAACTCTGATCAAGTATTGACGGATGATGATCAACCAATGACTACGCATACAACGAACCCAGTACCAGTGATTGTAACAAAAGAAGGCGTTACACTTagag < 2:396572/141‑1 (MQ=255) aGACATGGACGGTTGTGCAATTATTACTGCTGACCATGGTAACTCTGATCAAGTATTGACGGATGATGATCAACCAATGACTACGCATACAACGAACCCAGTACCAGTGATTGTAACAAAAGAAGGCGTTACACTTAgaga > 2:387387/1‑141 (MQ=255) aGACATGGACGGTTATGCAATTATTACTGCTGACCATGGTAACTCTGATCAAGTATTGACGGATGATGATCAACCAATGACTACGCATACAACGAACCCAGTACCAGTGATTGTAACAAAAGAAGGCGTTACACTTAgaga > 1:147686/1‑141 (MQ=255) gACATGGACGGTTATGCAATTATTACTGCTGACCATGGTAACTCTGATCAAGTATTGACGGATGATGATCAACCAATGACTACGCATACAACGAACCCAGTACCAGTGATTGTAACAAAAGAAGGCGTTACACTTAGAGaa > 2:130961/1‑141 (MQ=255) cATGGACGGTTATGCAATTATTACTGCTGACCATGGTAACTCTGATCAAGTATTGACGGATGATGATCAACCAATGACTACGCATACAACGAACCCAGTACCAGTGATTGTAACAAAAGAAGGCGTTACACTTAGAGAAAc > 1:406482/1‑141 (MQ=255) cATGGACGGTTATGCAATTATTACTGCTGACCATGGTAACTCTGATCAAGTATTGACGGATGATGATCAACCAATGACTACGCATACAACGAACCCAGTACCAGTGATTGTAACAAAAGAAGGCGTTACACTTAGAGAAAc > 2:422860/1‑141 (MQ=255) ggACGGTTATGCAATTATTACTGCTGACCATGGTAACTCTGATCAAGTATTGACGGATGATGATCAACCAATGACTACGCATACAACGAACCCAGTACCAGTGATTGTAACAAAAGAAGGCGTTACACTTAGAGAAACTgg > 1:175707/1‑141 (MQ=255) cGGTTATGCAATTATTACTGCTGACTATGGTAACTCTGATCAAGTATTGACGGATGATGATCAACCAATGACTACGCATACAACGAACCCAGTACCAGTGATTGTAACAAAAGAAGGCGTTACACTTAGAGAAACTGGTCg < 2:10675/141‑1 (MQ=255) tGCATTTATTACTGCTGACCATGGTAACTCTGATCAAGTATGGACGGATTATGATCAACTACTGACAAAGCATACAACGAACCCAGTACCCGGCAGAGTCACAAAAGAAGGCGTTACACTTAAAGCAACTGGTCGCTTAgg > 2:225373/1‑141 (MQ=255) gCAATTATTACTGCTGACCATGGTAACTCTGATCAAGTATTGACGGATGATGATCAACCAATGACTACGCATACAACGAACCCAGTACCAGTGATTGTAACAAAAGAAGGCGTTACACTTAGAGAAACTGGTCGCTTAggg > 2:329471/1‑140 (MQ=255) gCAATTATTACTGCTGACCATGGTAACTCTGATCAAGTATTGACGGATGATGATCAACCAATGACTACGCATACAACGAACCCAGTACCAGTGATTGTAACAAAAGAAGGCGTTACACTTAGAGAAACTGGTCGCTTAGGt < 1:144403/141‑1 (MQ=255) ttattaCTGCTGACCATGGTAACTCTGATCAAGTATTGACGGATGATGATCAACCAATGACTACGCATACAACGAACCCAGTACCAGTGATTGTAACAAAAGAAGGCGTTACACTTAGAGAAACTGGTCGCTTAGGTGACt < 1:245097/141‑1 (MQ=255) tgctgACCATGGTATCTCTGCTCAAGTATTGACGGATGATGATCAACCAATGACTACGCATACAACGAACCCAGTACCAGTGATTGTAACAAAAGAAGGCGTTACACTTAGAGAAACTGGTCGCGTAGGTGACTTAGCAcc < 1:284815/141‑1 (MQ=255) tgctgACCATGGTAACTCTGATCAAGTATTGACGGATGATGATCAACCAATGACTACGCATACAACGAACCCAGTACCAGTGATTGTAACAAAAGAAGGCGTTACACTTAGAGAAACTGGTCGCTTAGGTGACTTAGCAcc > 2:382219/1‑141 (MQ=255) gACCATGGTAACTCTGATCAAGTATTGACGGATGATGATCAACCAATGACTACGCATACAACGAACCCAGTACCAGTGATTGTAACAAAAGAAGGCGTTACACTTAGAGAAACTGGTCGCTTAGGTGACTTAGCACCTACa < 1:130961/141‑1 (MQ=255) ccATGGTAACTCTGATCAAGTATTGACGGATGATGATCAACCAATGACTACGCATACAACGAACCCAGTACCAGTGATTGTAACAAAAGAAGGCGTTACACTTAGAGAAACTGGTCGCTTAGGTGACTTAGCACCTACatt < 1:318067/141‑1 (MQ=255) tGGTAACTCTGATCAAGTATTGACGGATGATGATCAACCAATGACTACGCATACAACGAACCCAGTACCAGTGATTGTAACAAAAGAAGGCGTTACACTTAGAGAAACTGGTCGCTTAGGTGACTTAGCATCTACattatt < 1:414155/141‑1 (MQ=255) tGGTAACTCTGATCAAGTATTGACGGATGATGATCAACCAATGACTACGCATACAACGAACCCAGTACCAGTGATTGTAACAAAAGAAGGCGTTACACTTAGAGAAACTGGTCGCTTAGGTGACTTAGCACCTACattatt > 1:122817/1‑141 (MQ=255) tGGTAACTCTGATCAAGTATTGACGGATGATGATCAACCAATGACTACGCATACAACGAACCCAGTACCAGTGATTGTAACAAAAGAAGGCGTTACACTTAGAGAAACTGGTCGCTTAGGTGACTTAGCACCTACattatt < 1:381383/141‑1 (MQ=255) aCTCTGATCAAGTATTGACGGATGATGATCAACCAATGACTACGCATACAACGAACCCAGTACCAGTGATTGTAACAAAAGAAGGCGTTACACTTAGAGAAACTGGTCGCTTAGGTGACTTAGCACCTACATTATTAGAtt > 2:294206/1‑141 (MQ=255) tctGATCAAGTATTGACGGATGATGATCAACCAATGACTACGCATACAACGAATCCAGTACCAGTGATTGTAACAAAAGAAGGCGTTACGCTTAGAGAAACTGGTCGCTTAGGTGACTTAGCACCTACATTATTAGATtta < 2:404445/141‑1 (MQ=255) tctGATCAAGTATTGACGGATGATGATCAACCAATGACTACGCATACAACGAATCCAGTACCAGTGATTGTAACAAAAGAAGGCGTTACACTTAGAGAAACTGGTCGCTTAGGTGACTTAGCACCTACATTATTAGATtta < 2:404449/141‑1 (MQ=255) tctGATCAAGTATTGACGGATGATGATCAACCAATGACTACGCATACAACGAACCCAGTACCAGTGATTGTAACAAAAGAAGGCGTTACACTTAGAGAAGCTGGTCGCTTAGGTGACTACGCACCTACATTATTAGAttaa > 2:163160/1‑139 (MQ=255) tctGATCAAGTATTGACGGATGATGATCAACCAATGACTACGCATACAACGAACCCAGTACCAGTGATTGTAACAAAAGAAGGCGTTACACTTAGAGAAACTGGTCGCTTAGGTGACTTAGCACCTACATTATTAGATtta > 2:421939/1‑141 (MQ=255) gTATTGACGGATGATGATCAACCAATGACTACGCATACAACGAACCCAGTACCAGTGATTGTAACAAAAGAAGGCGTTACACTTAGAGAAACTGGTCGCTTAGGTGACTTAGCACCTACATTATTAGCTTTATTAAATGTa > 1:53661/1‑141 (MQ=255) aTTGACGGATGATGATCAACCAATGACTACGCATACAACGAACCCAGTACCAGTGATTGTAACAAAAGAAGGCGTTACACTTAGATAAACCGGTCGCTTAGGTGACTTAGCACCTACATTATTAGATTTATTAAATGTAGa < 1:474067/141‑1 (MQ=255) aTTGACGGATGATGATCAACCAATGACTACGCATACAACGAACCCAGTACCAGTGATTGTAACAAAAGAAGGCGTTACACTTAGAGAAACTGGTCGCTTAGGTGACTTAGCACCTACATTATTAGATTTATTAAATGTAGa > 1:561126/1‑141 (MQ=255) gACGGATGATGATCAACCAATGACTACGCATACAACGAACCCAGTACCAGTGATTGTAACAAAAGAAGGCGTTACACTTAGAGAAACTGGTCGCTTAGGTGACTTAGCACCTACATTATTAGATTTATTAAATGTAGaaca < 1:374492/141‑1 (MQ=255) gACGGATGATGATCAACCAATGACTACGCATACAACGAACCCAGTACCAGTGATTGTAACAAAAGAAGGCGTTACACTTAGAGAAACTGGTCGCTTAGGTGACTTAGCACCTACATTATTAGATTTATTAAATGTAGaaca > 1:312751/1‑141 (MQ=255) gACGGATGATGATCAACCAATGACTACGCATACAACGAACCCAGTACCAGTGATTGTAACAAAAGAAGGCGTTACACTTAGAGAAACTGGTCGCTTAGGTGAATTAGCACCTACATTATTGGCTTTATTGAATGTGGaaac > 1:490893/1‑139 (MQ=255) aCGGATGATGATCAACCAATGACTACGCATACAACGAACCCAGTACCAGTGATTGTAACAAAAGAAGGCGTTACACTTAGAGAAACTGGTCGCTTAGGTGACTTAGCACCTACATTATTAGATTTATTAAATGTAGaacaa > 2:383151/1‑141 (MQ=255) cGGATGATGATCAACCAATGACTACGCATACAACGAACCCAGTACCAGTGATTGTAACAAAAGAAGGCGTTACACTTAGAGAAACTGGTCGCTTAGGTGACTTAGCACCTACATTATTAGATTTATTAAATGTAGaacaac < 1:446358/141‑1 (MQ=255) gcatgatgatgAACCAATGACTACGCATACAACGAACCCAGTACCAGTGATTGGAACAAAAGAAGGCGTTCCACTTAGAGAAACTGGTCGCTTAGGTGACTTAGCACCTACATTATTAGATTTATTAAATGTAGAACAAcc < 2:315504/139‑1 (MQ=255) atgatgatCAACCAATGACTACGCATACAACGAACCCAGTACCAGTGATTGTAACAAAAGAAGGCGTTACACTTAGAGAAACTGGTCGCTTAGGTGACTTAGCACCTACATTATTAGATTTATTAAATGTAGAACAACCTg > 2:240662/1‑141 (MQ=255) tgatgaaCAACCAATGTCTACGCATACAACGAACCCAGTACCAGTGATTGTGTCAAAAGAAGGCGTTACACTTAGAGAAACTGGTCGCTTAGGTGACTTAGCACCTACATTATTAGATTTATTAAATGTAGAACAACCTGa < 1:130499/141‑1 (MQ=255) tCAACCAATGACTACGCATACAACGAACCCAGTACCAGTGATTGTAACAAAAGAAGGCGTTACACTTAGAGAAACTGGTCGCTTAGGTGACTTAGCACCTACATTATTAGATTTATTAAATGTAGAACAACCTGAAGatat < 1:363425/141‑1 (MQ=255) ccAATGACTACGCATACAACGAACCCAGTACCAGTGATTGTAACAAAAGAAGGCGTTACACTTAGAGAAACTGGTCGCTTAGGTGACTTAGCACCTACATTATTAGATTTATTAAATGTAGAACAACCTGAAGATATGACa > 2:162375/1‑141 (MQ=255) cAATGACTACGCATACAACGAACCCAGTACCAGTGATTGTGACAAAAGAAGGCGTTACGCTTAGAGAAACTGGTCGCTTAGGTGACTTAGCACCTACATTATTAGATTTATTAAATGTGGAACAACCTGAAGATATGACAg > 2:44239/1‑141 (MQ=255) cAAGGACTACGAAAACAACGAACCCAGTACCAGTGATTGTAACAAAAGAAGGCGTTACACTTAGAGAAACTGGTCGCTTAGGTGACTTAGCACCTACATTATTAGATTTATTAAATGTAGAACAACCTGAAGATATGACAg < 1:68787/141‑1 (MQ=255) gACTACGCATACAACGAACCCAGTACCAGTGATTGTAACTAAAGAAGGCGTTACACTTAGAGAAAATGGTCGCTTAGGTGACTTCGCACCTACATTATTAGATTTATTAAATGTAGGACAACCTGAAGATATGACAGGTGa > 2:282719/1‑141 (MQ=255) gACTACGCATACAACGAACCCAGTACCAGTGATTGTAACAAAAGAAGGCGTTACACTTAGAGAAACTGGTCGCTTAGGTGACTTAGCACCTACATTATTAGATTTATTAAATGTAGGACAACGTGACGATCTGAGAGGTGa > 1:162324/1‑141 (MQ=255) aCGCATACAACGAACCCAGTACCAGTGATTGTAACAAAAGAAGGCGTTACACTTAGAGAAACTGGTCGCTTAGGTGACTTAGCACCTACATTATTAGATTTATTAAATGTAGAACAACCTGAAGATATGACAGGTGAATCt < 1:489481/141‑1 (MQ=255) gCATACAACGAACCCAGTACCAGTGATTGTAACAAAAGAAGGCGTTACACTTAGAGAAACTGGTCGCTTAGGTGACTTAGCACCTACATTATTAGATTTATTAAATGTAGAACAACCTGAAGATATGACAGGTGAATCttt < 1:496029/141‑1 (MQ=255) tACAACGAACCCAGTACCAGTGATTGTAACAAAAGAAGGCGTTACACTTAGAGAAACTGGTCGCTTAGGTGACTTAGCACCTACATTATTAGATTTATTAAATGTAGAACAACCTGAAGATATGACAGGTGAATCTttaat < 1:382219/141‑1 (MQ=255) aataCGAACCCAGTACCAGTGATTGTAACAAAAGATCGCGATACGCGGAGAGAAACTGGTCGCTTAGGTGACTTAGCACCTACATTATTAGATTTATTAAATGGAGAACAACCGGAAGATATGACAGGTGAATCTttaatt < 1:13961/138‑1 (MQ=255) | GAAGCGGTTGATGAATGTTTAGGAGAAGTGGTTGATAAGATTTTAGACATGGACGGTTATGCAATTATTACTGCTGACCATGGTAACTCTGATCAAGTATTGACGGATGATGATCAACCAATGACTACGCATACAACGAACCCAGTACCAGTGATTGTAACAAAAGAAGGCGTTACACTTAGAGAAACTGGTCGCTTAGGTGACTTAGCACCTACATTATTAGATTTATTAAATGTAGAACAACCTGAAGATATGACAGGTGAATCTTTAATT > CP000730/861756‑862028 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |