Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 874,718 A→C 100% K541N (AAA→AAC clfA → fibrinogen‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730874,7180AC100.0% 196.8 / NA 61K541N (AAA→AACclfAfibrinogen‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (32/29);  total (32/29)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GATCCGAATAGCAAAGGTGATTTAGCTTTACGTTCAACTTTATATGGGTATAACTCGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAAACCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCGGTTCAGATTCGGGTAGTGATTCTACAT  >  CP000730/874581‑874854
                                                                                                                                         |                                                                                                                                        
gATCCGAATAGCAACGGTGATTTAGCTTTACGTTCAACTTTATATGGGTATAACCCGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGGTCAGGTGACGGTATCGATAAcccc                                                                                                                                       >  2:804/1‑140 (MQ=255)
gATCCGAATAGCAAAGGTGATTTAGCTTTACGTTCAACTTTATATGGGTATAACTCGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCa                                                                                                                                       <  2:316217/141‑1 (MQ=255)
 aTCCGAATAGCAAAGGTGATTTAGCTTTACGTTCAACTTTATATGGGTATAACTCGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAg                                                                                                                                      >  2:163823/1‑141 (MQ=255)
         aGCAAAGGTGATTTAGCTTTACGTTCAACTTTATATGGGTATAACTCGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACAGATCAGGCTCGGGTGACGGTATCGATAACCCAGTTGTTCCt                                                                                                                              >  1:245567/1‑141 (MQ=255)
           cAAAGGTGACTTAGCTTTACGTTCAACTTTATATGGGTATAACTCGAATATAATTTGGCGCTCTATGTCATGGGACAACGCAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTcagga                                                                                                                            <  1:249091/141‑5 (MQ=255)
                            tACGTTCAACTTTATATGGGTATAACTGGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTg                                                                                                           <  2:134722/141‑1 (MQ=255)
                                ttCAACTTTATATGGGTATAACTCGAATATAATTTGGCGCTCTATGTCATGGGACAACCAAGTAGCATTGAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGa                                                                                                       <  2:486764/141‑1 (MQ=255)
                                 tCAACTTTATATGGGTATAACTCGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGaa                                                                                                      >  2:186809/1‑141 (MQ=255)
                                      tttATATGGGTATAACTCGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTCATGAGCCTGGTGAAATTGa                                                                                                 >  2:202277/1‑141 (MQ=255)
                                           atGGGTATAACTCGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAAAAACGGATCAGGTTCTGGGGACGGTATCGATAACCCAGTTGTTCCTAAACAACCTGATGAGACTGGTGGAAATGAACCaa                                                                                            >  1:56301/1‑141 (MQ=255)
                                            tGGGTATAACTCGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACCAACTGACGGGCCTGGTGataaggattcaat                                                                                           >  1:556581/1‑129 (MQ=255)
                                            tGGGTATAACTCGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCGGAGGGGCCTCGTGCAAACGAAGCAAt                                                                                           >  1:556441/1‑141 (MQ=255)
                                             gggTATAACTCGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTGCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAAtt                                                                                          >  2:72750/1‑141 (MQ=255)
                                             gggTATAACTCGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGAGCACTCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGCAATTTAACCAAtt                                                                                          >  1:158728/1‑141 (MQ=255)
                                             gggTATAACTCGAATATAATTTGGCGCTCTATGTAATGCGACCCCCAACTAACATTTAAAAAAGGATCAGGTTCTGCACTCGCTATAGCTAACCCAGCTGTGCCGGAACAGCCTGATGAGTCTCGGGAAATGTAACCAAtt                                                                                          >  2:331717/1‑141 (MQ=255)
                                               gTATAACTCGAATCTAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTcc                                                                                        <  2:22575/141‑1 (MQ=255)
                                                  taACTCGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAAAAACCTGATGAGCCTGGTGAAATTGAACCAATTCAaga                                                                                     >  2:103162/1‑141 (MQ=255)
                                                         aaTATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCa                                                                              >  2:376353/1‑141 (MQ=255)
                                                          atatAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATCGAACCAATTCCAGAGGATTCAg                                                                             <  2:417930/141‑1 (MQ=255)
                                                              aaTTTGGCGCTCTAGGTCATGGGACAACGAAGTAGCATTTAATAAAGGAGCAGGAGCGGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTc                                                                         <  1:442468/141‑1 (MQ=255)
                                                                 ttGGCGCTCTATGTCATGGGACACCGAAGTAGCATTTCATAACGGATCAGGTTCTGGTTACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGCTGATCCTGGTGAAATTGAACAACTTCCAGAGGATTCAAATTCTGa                                                                      >  2:8411/1‑141 (MQ=255)
                                                                    gcgcTCTATGTCATGGGACAACGAAGTAGCATTTAATACTGGCTCAGCTTCGGGCGGCAGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGAccc                                                                   <  2:174167/141‑1 (MQ=255)
                                                                         ctATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGtt                                                              <  2:73352/141‑1 (MQ=255)
                                                                          tATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTc                                                             <  2:410425/141‑1 (MQ=255)
                                                                          tATGTCAGGGGCCAACGTAGTAGCATTTAAGAACGGACCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTc                                                             <  2:361119/141‑1 (MQ=255)
                                                                           aTGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCGTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCa                                                            >  2:178825/1‑141 (MQ=255)
                                                                            tGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAg                                                           >  2:539796/1‑141 (MQ=255)
                                                                              tCATGGGACGACGAAGTAGCATTTAATAACGGTTCAGGTTCTGGTGACGGTATCGATACCCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGAt                                                         <  2:68598/141‑1 (MQ=255)
                                                                                 tGGTACAACTAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGTCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCt                                                      <  2:438095/141‑1 (MQ=255)
                                                                                    gACAACGAAGTAGGATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGc                                                   <  2:210783/141‑1 (MQ=255)
                                                                                     aCAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTGTGACCCAGGGTCAGATCCTGGcg                                                  >  2:445261/1‑140 (MQ=255)
                                                                                       aaCGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGc                                                >  1:515433/1‑141 (MQ=255)
                                                                                         cGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCcgcgg                                              >  1:144475/1‑140 (MQ=255)
                                                                                            aGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTc                                           <  1:237038/141‑1 (MQ=255)
                                                                                               aGCATTTAATAACGTATCAGGTGCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTaa                                        <  1:181516/141‑1 (MQ=255)
                                                                                                  aTTTAATAACGGATCAGGGTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTc                                     <  2:66320/141‑1 (MQ=255)
                                                                                                       ataaCGGATCATGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGTACAACCTGATGAGCCTGGTGAAATTGAACCACTTCCAGCGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATa                                >  2:171952/1‑141 (MQ=255)
                                                                                                       ataaCGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATa                                >  1:163864/1‑141 (MQ=255)
                                                                                                       ataaCGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCACATa                                >  2:35276/1‑141 (MQ=255)
                                                                                                         aaCGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGc                              >  1:32440/1‑141 (MQ=255)
                                                                                                         aaCGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGAGAGc                              >  2:529796/1‑141 (MQ=255)
                                                                                                          aCGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGATCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCg                             >  1:349962/1‑141 (MQ=255)
                                                                                                           cGGATCAGGTTTTGGTGACGGTATCGATCACCCATTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCgg                            <  2:456210/141‑1 (MQ=255)
                                                                                                           cGGATCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATCCATATAGCgg                            >  2:46595/1‑141 (MQ=255)
                                                                                                             gaccAGGTTCTGGTGACGGTATCGATAACCCAGCTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCGGtt                          <  1:99673/138‑1 (MQ=255)
                                                                                                               tCAGGTTCTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAGGTCAGATAGTGGTGCa                        >  1:129126/1‑141 (MQ=255)
                                                                                                                      cTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACGTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCGGCGATTCTAATTCAGATAGCGGTTTAGagtaag                 >  1:10330/1‑136 (MQ=255)
                                                                                                                      cTGGTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCCGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCGGTTCAGCTTCgg                 >  1:131704/1‑141 (MQ=255)
                                                                                                                        ggTGGCGGTATGGATGACCCCGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAAGTGAACCCATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATGGCGGTTCAGATTCGGGt               <  2:221507/141‑1 (MQ=255)
                                                                                                                         gTGACGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCGGTTCAGATTCGGGTa              >  2:130716/1‑141 (MQ=255)
                                                                                                                            aCGGTTTCGATAACCCAGCGGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCGGTTCAGATTCGGGTAGTg           <  2:477983/141‑1 (MQ=255)
                                                                                                                             cGGTATCGATTACCCAGGTGCTACTGAACAACCTGATGACCCTGGTGAAATCGAACCAATTCCAGAGGATTCAGATGCTGACCCAGGTTCAGATTCTGGCCGCGATTCTAATTCAGATAGCCGTTCAGATTCGGGTAGTGa          <  2:41389/141‑1 (MQ=255)
                                                                                                                             cGGTATCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGAAAGCGGTTCAGATTCGGGTAGTGa          >  2:149598/1‑141 (MQ=255)
                                                                                                                                taccgctAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCGGTTCAGATTCGGGTAGTGATTc       <  1:37472/135‑1 (MQ=255)
                                                                                                                                tATCGATACCCCAGTTGTTCGTGAACAACCTGCGGGGCCCGGAGGGTTCGAGCCAATTCTAGAGGATTCATATTCGGGCCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCGGTTCAGATTCGGGTAGTGATTc       <  1:270021/141‑1 (MQ=255)
                                                                                                                                tATCGATAACCCAGTTGTTCCGGAACAACCTCATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCGGTTCAGATTCGGGTAGTGATTc       <  1:37456/141‑1 (MQ=255)
                                                                                                                                  tCGATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCGGTTCAGATTCGGGTAGTGATTCTa     >  2:429058/1‑141 (MQ=255)
                                                                                                                                   ccaTAACCCGGTTGTTCCTGAACAACCTTATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGCCCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCGGTTCAGATTCGGGTAGTGATTCTAc    <  2:98919/139‑1 (MQ=255)
                                                                                                                                    gATAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCGGTTCAGATTCGGGTAGTGATTCTACa   <  2:525840/141‑1 (MQ=255)
                                                                                                                                     aTAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCGGTTCAGATTCGGGTAGTGATTCTACAt  <  1:511802/141‑1 (MQ=255)
                                                                                                                                     aTAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCGGTTCAGATTCGGGTAGTGATTCTACAt  <  1:539796/141‑1 (MQ=255)
                                                                                                                                     aTAACCCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCGGGTCAGATTCGGGTGGTGATTCTACaa  >  2:154816/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                        
GATCCGAATAGCAAAGGTGATTTAGCTTTACGTTCAACTTTATATGGGTATAACTCGAATATAATTTGGCGCTCTATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGATAAACCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGATTCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCGGTTCAGATTCGGGTAGTGATTCTACAT  >  CP000730/874581‑874854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: