Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,238,026 T→A 9.4% L47H (CTT→CAT)  USA300HOU_1162 → glycerone kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,238,0260TA 9.4% 155.4 / 3.0 51L47H (CTT→CAT) USA300HOU_1162glycerone kinase
Reads supporting (aligned to +/- strand):  ref base T (21/24);  new base A (2/3);  total (23/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTAGCAAAATTAATGGTAAATTATTTGCCGATATGATTATACAAGGGGCACAAAATTTATCTAACAATGCAGATTTGGTAGATTCTTTGAATGTGTATCCAGTGCCAGATGGTGATACAGGAACAAATATGAATCTTACTATGACTTCAGGTCGCGAAGAAGTAGAGAATAATTTGTCGAAAAATATCGGCGAATTAGGTAAAACATTCTCGAAAGGTTTACTAATGGGTGCAAGAGGTAACTCTGGTGTCATCTTGTCACAATTATTCAGAGGA  >  CP000730/1237890‑1238165
                                                                                                                                        |                                                                                                                                           
aTTAGCAAAATTAATGGTAAATTATTTGCCGATATGATTATACAAGGGGCACAAGATTTATCTAACAATGCAGATTTGGTAGATTCTTTGAATGTGTATCCAGTGCCAGATGGTGATACAGGAACAAATATGAATCATACt                                                                                                                                         >  1:370777/1‑141 (MQ=255)
aTTAGCAAAATTAATGGTAAATTATTTGCCGATATGATTATACAAGGGGCACAAAATTTATCTAACAATGCAGATTTGGTAGATTCTTTGAATGTGTATCCAGTGCCAGATGGTGATACAGGAACAAATATGAATCATACt                                                                                                                                         >  1:370845/1‑141 (MQ=255)
      aaaaTTAATGGTAAATTATTTGCCGATATGATTATACAAGGGGCACAAAATTTATCTAACAATGCAGATTTGGTAGATTCTTTGAATGTGTATCCAGTGCCAGATGGTGATACAGGAACAAATATGAATCTTACTATGACt                                                                                                                                   <  1:273454/141‑1 (MQ=255)
             aTGGGAAATTATTTGCGGATATGATTATACAAGGGGCACAAAATTTAGCTAACAATGCAGATTTGGTAGATTCTTTGAATGTGTATCCAGTGCCAGATGGTGATACAGGAACAAATATGAATCTTACTATGACTTCAGGTc                                                                                                                            <  2:35776/141‑1 (MQ=255)
              tGGTAAATTATTTGCCGATATGATTATACAAGGGGCACAAAATTTATCTAACAATGCAGATTTGGTAGATTCTTTGAATGTGTATCCAGTGCCAGATGGTGGTACAGGAACAAATATGAATCTTACTATGACTTGAGGTcg                                                                                                                           >  1:330566/1‑141 (MQ=255)
                   aaTTATTTGCCGATATGATTATACAAGGGGCACAAAATTTATCTAACAATGCAGATTTGGTAGATTCTTTGAATGTGTATCCAGTGCCAGATGGTGATACAGGAACAAATATGAATCTTACTATGACTTCAGGTCGCgagg                                                                                                                      >  1:123711/1‑139 (MQ=255)
                      tattTGCCGATATGATTATACAAGGGGCACAAAATTTATCTAACAATGCAGATTTGGTAGATTCTTTGAATGTGTATCCAGTGCCAGATGGTGATACAGGAACAAATATGAATCTTACTATGACTTCAGCTCGCGaagaag                                                                                                                   >  2:483752/1‑141 (MQ=255)
                          tGCCGATATGATTATACAAGGGGCACAAAATTTATCTAACAATGCAGATTTGGTAGATTCTTTGAATGTGTATCCAGTGCCAGATGGTGATACAGGAACAAATATGAATCTTACTATGACTTCAGGTGGCGAAGAAGTaga                                                                                                               >  1:228006/1‑141 (MQ=255)
                          tGCCGATATGATTATACAAGGGGCACAAAATTTATCTAACAATGCAGATTTGGTAGATTCTTTGAATGTGTATCCAGTGCCAGATGGTGATACAGGAACAAATATGAATCTTACTATGACTTCAGGTCGCGAAGAAGTaga                                                                                                               >  2:367831/1‑141 (MQ=255)
                          tGCCGATATGATTATACAAGGGGCACAAAATTTATCTAACAATGCAGATTTGGTAGATTCTTTGAATGTGTATCCAGTGCCAGATGGTGATACAGGAACAAATATGAATCTTACTATGACTTCAGGTCGCGAAGAAGTaga                                                                                                               >  1:311209/1‑141 (MQ=255)
                            ccGATATGATTATACAAGGGGCACAAAATTTATCTAACAATGCAGATTTGGTAGATTCTTTGAATGTGTATCCAGTGCCAGATGGTGATACAGGAACAAATATGAATCTTACTATGACTTCAGGTCGCGAAGAAGTAgaga                                                                                                             <  2:399966/141‑1 (MQ=255)
                              gATATGATTATACAAGGGGCACAAAATTTATCTAACAATGCAGATTTGGTAGATTCTTTGAATGTGTATCCAGTGCCAGATGGTGATACAGGAACAAATATGAATCTTACTATGACTTCAGGTCGCGAAGAAGTAGAGaat                                                                                                           <  2:540651/141‑1 (MQ=255)
                                    aTTATACAAGGGGCACAAAATTTATCTAACAATGCAGATTTGGTAGATTCTTTGAATGTGTATCCAGTGCCAGATGGTGATACAGGAACAAATATGAATCTTACTATGACTTCAGGTCGCGAAGAAGTAGAGAATAATTTg                                                                                                     >  2:352158/1‑141 (MQ=255)
                                         aCAAGGGGCACAAAATTTATCTAACAATGCAGATTTGGTAGATTCTTTGAATGTGTATCCAGTGCCAGATGGTGATACAGGAACAAATATGAATCTTACTATGACTTCAGGTCGCGAAGAAGTAGAGAATAATTTGTCGaa                                                                                                <  2:227209/141‑1 (MQ=255)
                                             ggggCACAAAATTTATCTAACAATGCAGATTTGGTAGATTCTTTGAATGTGTATCCAGTGCCAGATGGTGATACAGGAACAAATATGAATCTTACTATGACTTCAGGTCGCGAAGAAGTAGAGAATAATTTGTCGAAAAat                                                                                            >  2:282224/1‑141 (MQ=255)
                                                gCACAAAATTTATCCAACAATGCAGATTTGGTAGATTCTTTGAATGTGTATCCAGTGCCAGATGGTGATACAGGAACAAATATGAATCTTACTATGACTTCAGGTCGCGAAGAAGTAGAGAATAATTTGTCGAAAAATATc                                                                                         <  1:499019/141‑1 (MQ=255)
                                                 cacaAAATTTATCTAACAATGCAGATTTGGTAGATTCTTTGAATGTGTATCCAGTGCCAGATGGTGATACAGGAACAAATATGAATCTTACTATGACTTCAGGTCGCGAAGAAGTAGAGAATAATTTGTCGAAAAATATCg                                                                                        <  1:354559/141‑1 (MQ=255)
                                                 cacaAAATTTATCTAACAATGCAGATTTGGTAGATTCTTTGAATGTGTATCCAGTGCCAGATGGTGATACAGGAACAAATATGAATCTTACTATGACTTCAGGTCGCGAAGAAGTAGAGAATAATTTGTCGAAAAATATCg                                                                                        <  1:247981/141‑1 (MQ=255)
                                                 cacaAAATTTATCTAACAATGCAGATTTGGTAGATTCTTTGAATGTGTATCCAGTGCCAGATGGTGATACAGGAACAAATATGAATCTTACTATGACTTCAGGTCGCGAAGAAGTAGAGAATAATTTGTCGAAAAATATCg                                                                                        >  2:504754/1‑141 (MQ=255)
                                                 cacaAAATTTATCTAACAATGCAGATTTGGTAGATTCTTTGAATGTGTATCCAGTGCCAGATGGTGATACAGGAACAAATATGAATCTTACTATGACTTCAGGTCGCGAAGAAGTAGAGAATAATTTGTCGAAAAATATCg                                                                                        <  1:533030/141‑1 (MQ=255)
                                                 cacaAAATTTATCTAACAATGCAGACTTGGTAGATTCTTTGAATGTGTATCCAGTGCCAGATGGTGATACAGGAACAAATATGGAAAAAATTACGCCTTTCGGCCTTTTATAAGTAGAGAATAATTTGTCGATAAATATCg                                                                                        <  1:562668/141‑1 (MQ=255)
                                                  acaAAATTTATCTAACAATGCAGATTTGGTAGATTCTTTGAATGTGTATCCAGTGCCAGATGGTGATACAGGAACAAATATGAATCTTACTATGACTTCAGGTCGCGAAGAAGTAGAGAATAATTTGTCGAAAAATATCgg                                                                                       <  2:104501/141‑1 (MQ=255)
                                                  acaAAATTTATCTAACAATGCAGAGTTGGTAGATTCTTTGAATGTGTATCCAGTGCCAGATGGTGATACAGGAACAAATATGAATCTTACTATGACTTCAGGTCGCGAAGAAGTAGAGAATAATTTGTCGAAAAATAGCgg                                                                                       >  1:201474/1‑141 (MQ=255)
                                                     aaaTTTATCTAACAATGCAGATTTGGTAGATTCTTTGAATGTGTATCCAGTGCCAGATGGTGATACAGGAACAAATATGAATCTTACTATGACTTCAGGTCGCGAAGAAGTACAGAATAATTTGTCCAAAAATATCGGCGa                                                                                    <  1:372929/141‑1 (MQ=255)
                                                             cTAACAATGCAGATTTGGTAGATTCTTTGAATGTGTATCCAGTGCCAGATGGTGATACAGGAACAAATATGAATCTTACTATGACTTCAGGTCGCGAAGAAGTAGAGAATAATTTGTCGAAAAATATCGGCGAATTAGGTa                                                                            <  2:20488/141‑1 (MQ=255)
                                                                 cAATGCAGATTTGGTAGATTCTTTGAATGTGTATCCAGTGCCAGATGGTGATACAGGAACAAATATGAATCTTACTATGACTTCAGGTCGCGAAGAAGTAGAGAATAATTTGTCGAAAAATATCGGCGAATTAGGTAAAAc                                                                        <  2:311209/141‑1 (MQ=255)
                                                                 cAATGCAGATTTGGTAGATTCTTTGAATGTGTATCCAGTGCCAGATGGTGATACAGGAACAAATATGAATCTTACTATGACTTCAGGTCGCGAAGAAGTAGAGAATAATTTGTCGAAAAATATCGGCGAATTAGGTAAAAc                                                                        <  2:174756/141‑1 (MQ=255)
                                                                     gCAGATTTGGTAGATTCTTTGAATGTGTATCCAGTGCCAGATGGTGATACAGGAACAAATATGAATCTTACTATGACTTCAGGTCGCGAAGAAGTAGAGAATAATTTGTCGAAAAATATCGGCGAATTAGGTAAAACATtc                                                                    >  2:503896/1‑141 (MQ=255)
                                                                      ggtgattGGTAGTATGTTTGAATGTGTATCCAGGTGCAGTTGGGTATTCGTGAACACATATGAATCGTACTCTGACTTCAGGTCGCGAATAAGTAGAGAAGAAGTTGTCGAAAAATATCGGCGAATTAGGTTAAACATtcg                                                                   <  2:435316/136‑2 (MQ=255)
                                                                         aTTTGGTAGATTCTTTGAATGTGTATCCAGTGCCAGATGGTGATACAGGAACAAATATGAATCTTACTATGACTTCAGGTCGCGAAGAAGTAGAGAATAATTTGTCGAAAAATATCGGCGAATTAGGTAAAACATTCTCGa                                                                <  2:414627/141‑1 (MQ=255)
                                                                         aTTTGGTAGATTCTTTGAATGTGTATCCAGTGCCAGATGGTGATACAGGAACAAATATGAATCTTACTATGACTTCAGGTCGCGAAGAAGTAGAGAATAATTTGTCGAAAAATATCGGCGAATTAGGTAAAACATTCTCGa                                                                >  2:494131/1‑141 (MQ=255)
                                                                          tttGGTAGATTCTTTGAATGTGTATCCAGTGCCAGATGGTGATACAGGAACAAATATGAATCTTACTATGACTTCAGGTCGCGAAGAAGTAGAGAATAATTTGTCGAAAAATATCGGCGAATTAGGTAAAAAATTATCGaa                                                               >  2:56127/1‑141 (MQ=255)
                                                                            tGGTAGATTCTTTGAATGTGTATCCAGTGCCAGATGGTGATACAGGAACAAATATGAATCTTACTATGACTTCAGGTCGCGAAGAAGTAGAGAATAATTTGTCGAAAAATATCGGCGAATTAGGTAAAACATTCTCGAAAg                                                             <  2:513040/141‑1 (MQ=255)
                                                                                 gATTCTTTGAATGTGTATCCAGTGCCAGATGGTGATACAGGAACAAATATGAATCTTACTATGACTTCAGGTCGCGAAGAAGTAGAGAATAATTTGTCGAAAAATATCGGCGAATTAGGTAAAACATTCTCGAAAGGTTTa                                                        <  1:352158/141‑1 (MQ=255)
                                                                                     ttttGAATGTGTAACCAGTGCCAGATGGTGATACAGGAACAAATATGAATCTTACTACGACTTCAGGTCGCGAAGAAGTAGAGAATAATTTGTCGAAAAATATCGGCGAATTAGGTAAAACATTCTCGAAAGGTTTACTaa                                                    <  2:434591/140‑1 (MQ=255)
                                                                                            tgtgTATCCAGTGCCAGATGGTGATACAGGAACAAATATGAATCTTACTATGACTTCAGGTCGCGAAGAAGTAGAGAATAATTTGTCGAAAAATATCGGCGAATTAGGTAAAACATTCTCGAAAGGTTTACTAATGGATga                                             >  1:187321/1‑140 (MQ=255)
                                                                                                tATCCAGTGCCAGATGGTGATACAGGAACAAATATGAATCTTACTATGACTTCAGGTCGCGAAGAAGTAGAGAATAATTTGTCGAAAAATATCGGCGAATTAGGTAAAACATTCTCGAAAGGTTTACTAATGGGTGCAaga                                         >  1:263294/1‑141 (MQ=255)
                                                                                                 aTCCAGTGCCAGATGGTGATACAGGAACAAATATGAATCTTACTATGACTTCAGGTCGCGAAGAAGTAGAGAATAATTTGTCGAAAAATATCGGCGAATTAGGTAAAACATTCTCGAAAGGTTTACTAATGGGTGCAagag                                        <  1:367831/141‑1 (MQ=255)
                                                                                                 aTCCAGTGCCAGATGGTGATACAGGAACAAATATGAATCTTACTATGACTTCAGGTCGCGAAGAAGTAGAGAATAATTTGTCGAAAAATATCGGCGAATTAGGTAAAACATTCTCGAAAGGTTTACTAATGGGTGCAagag                                        <  1:85841/141‑1 (MQ=255)
                                                                                                      gTGCCAGATGGTGATACAGGAACAAATATGAATCTTACTATGACTTCAGGTCGCGAAGAAGTAGAGAATAATTTGTCGAAAAATATCGGCGAATTAGGTAAAACATTCTCGAAAGGTTTACTAATGGGTGCAAGAGGTAAc                                   <  1:504454/141‑1 (MQ=255)
                                                                                                        gCCAGATGGTGATACAGGAACAAATATGAATCTTACTATGACTTCAGGTCGCGAAGAAGTAGAGAATAATTTGTCGAAAAATATCGGCGAATTAGGTAAAACATTCTCGAAAGGTTTACTAATGGGTGCAAGAGGTAAcac                                 >  1:145992/1‑139 (MQ=255)
                                                                                                         ccAGATGGTGATACAGGAACAAATATGAATCTTACTATGACTTCAGGTCGCGAAGAAGTAGAGAATAATTTGTCGAAAAATATCGGCGAATTAGGTAAAACAGTCTCGAAAGGTTTACTAATGGGTGCAAAAGGTAActct                                >  2:437750/1‑141 (MQ=255)
                                                                                                            gATGGTGATACAGGAACAAATATGAATCTTACTATGACTTCAGGTCGCGAAGAAGTAGAGAATAATTTGTCGAAAAATATCGGCGAATTCGGTAAAACATTCTCGAAAGGTTTACTAAGGGGTGCAAGAGGTAACTCTGgt                             >  1:413060/1‑141 (MQ=255)
                                                                                                            gATGGTGATACAGGAACAAATATGAATCTTACTATGACTTCAGGTCGCGAAGAAGTAGAGAATAATTTGTCGAAAAATATCGGCGAATTAGGTAAAACATTCTCGAAAGGTTTACTAATGGGTGCAAGAGGTAACTCTGgt                             >  1:57520/1‑141 (MQ=255)
                                                                                                                  gATACAGGAACAAATATGAATCTTACTATGACTTCAGGTCGCGAAGAAGTAGAGAATAATTTGTCGAAAAATATCGGCGAATTAGGTAAAACATTCTCGAAAGGTTTACTAATGGGTGCAAGAGGTAACTCTGGTGTCATc                       >  1:5764/1‑141 (MQ=255)
                                                                                                                           aCAAATATGAATCTTACTATGACTTCAGGTCGCGAAGAAGTAGAGAATAATTTGTCGAAAAATATCGGCGAATTAGGTAAAACATTCTCGAAAGGTTTACTAATGGGTGCAAGAGGTAACTCTGGTGTCATCTTGTCACaa              <  1:282224/141‑1 (MQ=255)
                                                                                                                           aCAAATATGAATCTTACTATGACTTCAGGTCGCGAAGAAGTAGAGAATAATTTGTCGAAAAATATCGGCGAATTAGGTAAAACATTCTCGAAAGGTTTACTAATGGGTGCAAGAGGTAACTCTGGTGTCATCTTGTCACaa              >  1:84858/1‑141 (MQ=255)
                                                                                                                                aatGAATCTTACTATGACTTCAGGTCGCGAAGAAGTAGAGCATAATTTGTCGAAAAAGATCGGCGAATTAGGTAAAACATTCTCGATAGGTTTACTAATGGGTGCAAGAGGTAACTCTGGTGTCATCTTGTCACAattatt         <  1:474421/140‑1 (MQ=255)
                                                                                                                                   gAATCATACTATGACTTCAGGTCGCGAAGAAGTAGAGAATAATTTGTCGAAAAATATCGGCGAATTAGGTAAAACATTCTCGAAAGGTTTACTAATGGGTGCAAGAGGTAACTCTGGTGTCATCTTGTCACAATTATTCag      <  2:370845/141‑1 (MQ=255)
                                                                                                                                   gAATCATACTATGACTTCAGGTCGCGAAGAAGTAGAGAATAATTTGTCGAAAAATATCGGCGAATTAGGTAAAACATTCTCGAAAGGTTTACTAATGGGTGCAAGAGGTAACTCTGGTGTCATCTTGTCACAATTATTCag      <  2:370777/141‑1 (MQ=255)
                                                                                                                                       cTTACTATGACTTCAGGTCGCGAAGAAGTAGAGAATAATTTGTCGAAAAATATCGGCGAATTAGGTAAAACATTCTCGAAAGGTTTACTAATGGGTGCAAGAGGTAACAGTGGTGTCATCTTGTCACAATTATTCAGAGGa  <  1:564521/141‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                           
ATTAGCAAAATTAATGGTAAATTATTTGCCGATATGATTATACAAGGGGCACAAAATTTATCTAACAATGCAGATTTGGTAGATTCTTTGAATGTGTATCCAGTGCCAGATGGTGATACAGGAACAAATATGAATCTTACTATGACTTCAGGTCGCGAAGAAGTAGAGAATAATTTGTCGAAAAATATCGGCGAATTAGGTAAAACATTCTCGAAAGGTTTACTAATGGGTGCAAGAGGTAACTCTGGTGTCATCTTGTCACAATTATTCAGAGGA  >  CP000730/1237890‑1238165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑