Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,416,458 A→T 100% I216N (ATT→AAT)  USA300HOU_1314 ← oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,416,4580AT100.0% 100.9 / NA 32I216N (ATT→AAT) USA300HOU_1314oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (16/16);  total (16/16)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAAATGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTG‑ATTACCGTTCAAAGCATCCAATGATGCAACTGGTTC  >  CP000730/1416333‑1416594
                                                                                                                             |                                                                                                                                         
cATAGTAATCCCTCTTTGATTGGGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATAtt                                                                                                                            <  1:385838/141‑1 (MQ=255)
    gTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCaa                                                                                                                        <  2:157849/141‑1 (MQ=255)
    gTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCaa                                                                                                                        >  2:183627/1‑141 (MQ=255)
            tctTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTAACAATATTTCAATACTTGAc                                                                                                                >  2:351525/1‑141 (MQ=255)
            cctTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGAc                                                                                                                <  1:49664/140‑1 (MQ=255)
              tttAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACtt                                                                                                              >  2:544055/1‑141 (MQ=255)
              tttAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACtt                                                                                                              >  2:287925/1‑141 (MQ=255)
              tttAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGAAtt                                                                                                              >  2:287930/1‑141 (MQ=255)
                       gtTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTAtgt                                                                                                     >  1:541085/1‑141 (MQ=255)
                        ttCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTCAAGGTTTCACGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTAtgtg                                                                                                    <  1:132688/141‑1 (MQ=255)
                         tCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTAtgtgt                                                                                                   <  1:470025/141‑1 (MQ=255)
                              atTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGataa                                                                                              <  1:183627/141‑1 (MQ=255)
                                           ttCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTg                                                                                 <  1:382810/141‑1 (MQ=255)
                                            tCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGa                                                                                >  1:240030/1‑141 (MQ=255)
                                                              ggcccagacctgcaaTGAATAAGATTACCTTGTTCAATGACTTCACGTTCCTTTAAAACATAATTGTACTGACAACATTTCTGTACCTGACTTAAGTTATGTGTGATAATAACGAATGTTGGACCATGTTCTAATACAata                                                              <  2:105474/128‑1 (MQ=255)
                                                                cTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTTTGATAAGAAATAATGTTTGACCATGTTCTAATACAATATg                                                            >  1:387029/1‑141 (MQ=255)
                                                                 tCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGc                                                           <  1:293532/141‑1 (MQ=255)
                                                                            aTGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATc                                                >  1:257553/1‑141 (MQ=255)
                                                                               aTTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCAt                                             >  2:230449/1‑141 (MQ=255)
                                                                               aTTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCAt                                             >  2:340610/1‑141 (MQ=255)
                                                                                  aaTATTATCTCTTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATGATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCAc                                          <  2:349645/141‑1 (MQ=255)
                                                                                               tCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAATTAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTG‑ATTACCGTTc                            >  2:368163/1‑141 (MQ=255)
                                                                                                 aaTGATTTGACCTTCTTTTTAGACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTG‑ATTACCGTTCaa                          >  1:137941/1‑141 (MQ=255)
                                                                                                  aTGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTC‑ATTATGATTCaaa                         >  2:542780/1‑141 (MQ=255)
                                                                                                       ttGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGATATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTG‑ATTACCGTTCAAAGCATc                    >  2:482074/1‑141 (MQ=255)
                                                                                                        tGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTG‑ATTACAGTTCAAAGCATca                   >  1:185732/1‑140 (MQ=255)
                                                                                                          aCCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTG‑ATTACCGTTCAAAGCATCCaa                 <  1:534523/141‑1 (MQ=255)
                                                                                                           ccTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATACATCCA‑CGCGTGCATTACCGTTCAAAGCATCCAat                <  1:420078/141‑1 (MQ=255)
                                                                                                                         aTAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTG‑ATTACCGTTCAAAGCATCCAATGATGCAACTGGTTc  <  1:448770/141‑1 (MQ=255)
                                                                                                                         aTAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTG‑ATTACCGTTCAAAGCATCCAATGATGCAACTGGTTc  <  1:446387/141‑1 (MQ=255)
                                                                                                                         aTAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTG‑ATTACCGTTCAAAGCATCCAATGATGCAACTGGTTc  <  1:292261/141‑1 (MQ=255)
                                                                                                                         aTAATTGGACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTG‑ATTACCGTTCAAAGCATCCAATGATGCAACTGGTTc  <  2:72632/141‑1 (MQ=255)
                                                                                                                             |                                                                                                                                         
CATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAAATGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTG‑ATTACCGTTCAAAGCATCCAATGATGCAACTGGTTC  >  CP000730/1416333‑1416594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑