| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 2,810,588 | T→C | 10.2% | S266G (AGC→GGC) | secY2 ← | Sec family Type I general secretory pathway protein SecY |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 2,810,588 | 0 | T | C | 10.2% | 106.9 / 3.0 | 39 | S266G (AGC→GGC) | secY2 | Sec family Type I general secretory pathway protein SecY |
| Reads supporting (aligned to +/- strand): ref base T (12/23); new base C (2/2); total (14/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.09e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01 | |||||||||||
CTTTAAGAAATCTTTGGATTTTTGTTTAGTATTGATTAAAAATCTCGATAAAAAATAACCCAATAACATTTGAATCACTAAATATACTGAAATACCTACTGGACTATCAAATGTCAGCATTGGCATGTCATCTGATATGCTTTTATTAAACATAGATAAAATAAAATGAATGCCACTTTTTAAGAAAACAAAAGCTGAAATACTCATCATTAAAGTAATACTGCCTGCAGGGTTAACTTTCCAAGATAAATAAGATTTCATATTTGTTGCGGAAACGTTC > CP000730/2810448‑2810727 | ctTTAAGAAATCTTTGGATTTTTGTTTAGTATTGATTAAAAATCTCGATAAAAAATAACCCAATAACATTTGAATCACTAAATATACTGAAATACCTACTGGACTATCAAATGTCAGCATTGGCATGTCATCTGATATGCt > 1:456859/1‑141 (MQ=255)ctTTAAGAAATCTTTGGATTTTTGTTTAGTATTGATTAAAAATCTCGATAAAAAATAACCCAATAACATTTGAATCACTAAATATACTGAAATACCTACTGGACTATCAAATGTCAGCATTGGCATGTCATCTGATATGCt > 1:41536/1‑141 (MQ=255)ctTTAAGAAATCTTTGGATTTTTGTTTAGTATTGATTAAAAATCTCGATAAAAAATAACCCAATAACATTTGAATCACTAAATATACTGAAATACCTACTGGACTATCAAATGTCAGCATTGGCATGTCATCTGATATGCt < 1:528957/141‑1 (MQ=255) tttAAGAAATCTTTGGATTTTTGTTTAGTATTGATTAAAAATCTCGATAAAAAATAACCCAATAACATTTGAATCACTAAATATACTGAAATACCTACTGGACTATCAAATGTCAGCATTGGCATGTCATCTGATATGCtt > 1:231148/1‑141 (MQ=255) ggATTTTTGTTTAGTATTGATTAAAAATCTCGATAAAAAATAACCCAATAACATTTGAATCACTAAATATACTGAAATACCTACTGGACTATCAAATGTCAGCATTGGCATGTCATCTGATATGCTTTTATTAAACATAGa > 2:328961/1‑141 (MQ=255) gATTTTTGTTTAGTATTGATTAAAAATCTCGATAAAAAATAACCCAATAACATTTGAATCACTAAATATACTGAAATACCTACTGGACTATCAAATGTCAGCATTGGCATGTCATCTGATATGCTTTTATTAAACATAGat < 1:285261/141‑1 (MQ=255) tAGTATTGATTAAAAATCTCGATAAAAAATAACCCAATAACATTTGAATCACTAAATATACTGAAATACCTACTGGACTATCAAATGTCAGCATTGGCATGTCATCTGATATGCTTTTATTAAACATAGATaaaataaaat > 1:76757/1‑141 (MQ=255) aTTGATTAAAAATCTCGATAACAAATAACCCAATAACATTTGAATCACTAAATATACTGAAATACCTACTGGACTATCAAATGTCAGCATTGGCATGTCATCTGATATGCTTTTATTAAACATAGATAAAATAAaatgaat < 1:88806/141‑1 (MQ=255) ttGATTAAAAATCTCGATAAAAAATAACCCAATAACATTTGAATCACTAAATATACTGAAATACCTACTGGACTATCAAATGTCAGCATTGGCATGTCATCTGATATGCTTTTATTAAACATAGATAAAATAAaatgaatg < 1:334510/141‑1 (MQ=255) ttGATTAAAAATCTCGATAAAAAATAACCCAATAACATTTGAATCACTAAATATACTGAAATACCTACTGGACTATCAAATGTCAGCATTGGCATGTCATCTGATATGCTTTTATTAAACATAGATAAAATAAaatgaatg < 2:547991/141‑1 (MQ=255) tctcGATAAAAAATAACCCAATAACATTTGAATCACTAAATATACTGAAATACCTACTGGACTATCAAATGTCAGCATTGGCATGTCATCTGATATGCTTTTATTAAACATAGATAAAATAAAATGAATGCCACTTTTTaa > 2:130673/1‑141 (MQ=255) ctcGATAAAAAATAACCCAATAACATTTGAATCACTAAATATACTGAAATACCTACTGGACTATCAAATGTCAGCATTGGCATGTCATCTGATATGCTTTTATTAAACATAGATAAAATAAAATGAATGCCACTTTTTAAg < 1:63446/141‑1 (MQ=255) ctcGATAAAAAATAACCCAATAACATTTGAATCACTAAATATACTGAAATACCTACTGGACTATCAAATGTCAGCATTGGCATGTCATCTGATATGCTTTTATTAAACATAGATAAAATAAAATGAATGCCACTTTTTAAg < 2:20214/141‑1 (MQ=255) tAAAAAATAACCCAATAACATTTGAATCACTAAATATACTGAAATACCTACTGGACTATCAAATGTCAGCATTGGCATGTCATCTGATATGCTTTTATTAAACATAGATAAAATAAAATGAATGCCACTTTTTAAGAAAAc > 1:499434/1‑141 (MQ=255) tAACCCAATAACATTTGAATCACTAAATATACTGAAATACCTACTGGACTATCAAATGTCAGCATTGGCATGTCATCTGATATGCTTTTATTAAACATAGATAAAATAAAATGAATGCCACTTTTTAAGAAAACAAAAGCt > 1:67206/1‑141 (MQ=255) tAACCCAATAACATTTGAATCACTAAATATACTGAAATACCTACTGGACTATCAAATGTCAGCATTGGCATGTCATCTGATATGCTTTTATTAAACATAGATAAAATAAAATGAATGCCACTTTTTAAGAAAACAAAAGCt < 1:350060/141‑1 (MQ=255) aCCCAATAACATTTGAATCACTAAATATACTGAAATACCTACTGGACTATCAAATGTCAGCATTGGCATGTCATCTGATATGCTTTTATTAAACATAGATAAAATAAAATGAATGCCACTTTTTAAGAAAACAAAAGCTGa > 2:367963/1‑141 (MQ=255) aaCATTTGAATCACTAAATATACTGAAATACCTACTGGACTATCAAATGTCAGCATTGGCATGTCATCTGATATGCTTTTATTAAACATAGATAAAATAAAATGAATGCCACTTTTTAAGAAAACAAAAGCTGAAATACtc < 1:15186/141‑1 (MQ=255) tttGAATCACTAAATATACTGAAATACCTACTGGACTATCAAATGTCAGCATTGGCATGTCATCTGATATGCTTTTATTAAACATAGATAAAATAAAATGAATGCCACTTTTTAAGAAAACAAAAGCTGAAATACtcatca < 2:127122/141‑1 (MQ=255) acACTAAATAGACTGAACTACCTACGTGACTATCAAATGTCAGCATTGGCATGTCATCTGATATGCTTTTATTAAACATAGATAAAATAAAATGAATGCCACTTTTTAAGAAAACAAAAGCTGAAATACTCATCATTAAAg < 1:243595/140‑1 (MQ=255) cACTACATATACTGAAATACCTACTGGACTATCAAATGTCAGCATTGGCATGTCATCTGATATGCTTTTATTAAACATAGATAAAATAAAATGAATGCCACTTTTTAAGAAAACAAAAGCTGAAATACTCATCATTAAAGt < 1:265666/141‑1 (MQ=255) aaTATACTCAAATACCGACTGCACTATCAAATGTCAGCATTGGCATGTCATCTGATATGCTTTTATTAAACATAGATAAAATAAAATGAATGCCACTTTTTAAGAAAACAAAAGCTGAAATACTCATCATTAAAGTAATAc < 1:473298/141‑1 (MQ=255) cTGAAATACCTACTGGACTATCAAATGTCAGCATTGGCATGTCATCTGATATGCCTTTATTAAACATAGATAAAATAAAATGAATGCCACTTTTTAAGAAAACAAAAGCTGAAATACTCATCATTAAAGTAATActgcctg > 2:283421/1‑141 (MQ=255) cTGAAATACCTACTGGACTATCAAATGTCAGCATTGGCATGTCATCTGATATGCCTTTATTAAACATAGATAAAATAAAATGAATGCCACTTTTTAAGAAAAAAAAAGCTGAAATACTCATCATTAAAGTAATActgcctg > 2:150197/1‑141 (MQ=255) aCCTACGGGACTATCAAATGTCAGCCTTGGCATCTCCTCGTATATGCTTTTATTAAACATAGATAAAATAAAATGAATGCCACTTTTTAAGAAAACAAAAGCTGAAATACTCATCATTAAAGTAATACTGCCTGCAGGGtt < 1:156223/141‑1 (MQ=255) ccTACTGGACTATCAAATGTCAGCATTGGCATGTCATCTGATATGCTTTTATTAAACATAGATAAAATAAAATGAATGCCACTTTTTAAGAAAACAAAAGCTGAAATACTCATCATTAAAGTAATACTGCCTGCAGGGTTa < 1:83409/141‑1 (MQ=255) ccTACTGGACTATCAAATGTCAGCATTGGCATGTCATCTGATATGCTTCTATTCAATATAGATAAAATAAAATGAATGCCAGTTTTTAAGAAAACAAAAGCTGAAATACTCATCATTAAAGTAATTCTGCCTGCAGGGTTa < 1:421852/141‑1 (MQ=255) aTCAAATGTCAGCATTGGCATGTCATCTGATATGCTTTTATTAAACATAGATAAAATAAAATGAATGCCACTTTTTAAGAAAACAAAAGCTGAAATACTCATCATTAAAGTAATACTGCCTGCAGGGTTAACTTTCCAAGa < 1:328961/141‑1 (MQ=255) tCAAACGTCAGCATAGGCAGCTTATCTGATATGCTTTTATTAAACATAGATAAAATAAAATGAATGCCACTTTTTAAGAAAACAAAAGCTGAAATACTCATCATTAAAGTAATACTGCCTGCAGGGTTAACTTTCCAAGat < 1:215312/141‑1 (MQ=255) aaaTGTCAGCATTGGCATGTCATCTGATATGCTTTTATTAAACATAGATAAAATAAAATGAATGCCACTTTTTAAGAAAACAAAAGCTGAAATACTCATCATTAAAGTGATACTGCCTGCAGGGTTAACTTTCCAAGataa < 2:262753/141‑1 (MQ=255) aaaTGTCAGCATTGGCATGTCATCTGATATGCTTTTATTAAACATAGATAAAATAAAATGAATGCCACTTTTTAAGAAAACAAAAGCTGAAATACTCATCATTAAAGTAATACTGCCTGCAGGGTTAACTTTCCAAGttaa < 2:208840/141‑1 (MQ=255) aaaTGTCAGCATTGGCATGTCATCTGATATGCTTTTATTAAACATAGATAAAATAAAATGAATGCCACTTTTTAAGAAAACAAAAGCTGAAATACTCATCATTAAAGTAATACTGCCTGCAGGGTTAACTTTCCAAGataa < 1:84188/141‑1 (MQ=255) aTGTCAGCATTGGCATGTCATCTGATATGCTTTTATTAAACATAGATAAAATAAAATGAATGCCACTTTTTAAGAAAACAAAAGCTGAAATACTCATCATTAAAGTAATACTGCCTGCAGGGTTAACTTTCCAAGataaat > 2:28936/1‑141 (MQ=255) aGCATTGGCATGTCATCTGATATGCCTTTATTAAACATAGATAAAATAAAATGAATGCCACTTTTTAAGAAAACAAAAGCTGAAATACTCATCATTAAAGTAATACTGCCTGCAGGGTTAACTTTCCAAGATAAATAAGAt < 1:150197/141‑1 (MQ=255) aGCATTGGCATGTCATCTGATATGCCTTTATTAAACATAGATAAAATAAAATGAATGCCACTTTTTAAGAAAACAAAAGCTGAAATACTCATCATTAAAGTAATACTGCCTGCAGGGTTAACTTACCAAGATAAATAAGAt < 1:283421/141‑1 (MQ=255) cATTGGCATGTCATCTGATATGCTTTTATTAAACATAGATAAAATAAAATGAATGCCACTTTTTAAGAAAACAAAAGCTGAAATACTCATCATTAAAGTAATACTGCCTGCAGGGTTAACTTTCCAAGATAAATAAGAttt > 1:172296/1‑141 (MQ=255) ttGGCATGTCATCTGATATGCTTTTATTAAACATAGATAAAATAAAATGAATGCCACTTTTTAAGAAAACAAAAGCTGAAATACTCATCATTAAAGTAATACTGCCTGCAGGGTTAACTTTCCAAGATAAATAATATTTCa > 2:82683/1‑141 (MQ=255) ggCATGTCATCTGATATGCTTTTATTAAACATAGATAAAATAAAATGAATGCCACTTTTTAAGAAAACAAAAGCTGAAATACTCATCATTAAAGTAATACTGCCTGCAGGGTTAACTTTCCAAGATAAATAAGATTTCata > 2:95873/1‑141 (MQ=255) cTGATATGCTTTTATTAAACATAGATAAAATAAAATGAATGCCACTTTTTAAGAAAACAAAAGCTGAAATACTCATCATTAAAGTAATACTGCCTGCAGGGTTAACTTTCCAAGATAAATAAGATTTCATATTTGTTGCgg > 1:413572/1‑141 (MQ=255) tatGCTTTTATTAAACATAGATAAAATAAAATGAATGCCACTTTTTAAGAAAACAAAAGCTGAAATACTCATCATTAAAGTAATACTGCCTGCAGGGTTAACTTTCCAAGATAAATAAGATTTCATATTTGTTGCGGAAAc > 2:384278/1‑141 (MQ=255) tGCTTTTATTAAACATAGATAAAATAAAATGAATGCCACTTTTTAAGAAAACAAAAGCTGAAATACTCATCATTAAAGTAATACTGCCTGCAGGGTTAACTTTCCAAGATAAATAAGATTTCATATTTGTTGCGGAAACGt < 2:456859/141‑1 (MQ=255) tGCTTTTATTAAACATAGATAAAATAAAATGAATGCCACTTCTTAAGAAAACAAAAGCTGAAATACTCATCATTAAAGTAATACTGCCTGCAGGGTTAACTTTCCAAGATAAATAAGATTTCATATTTGTTGCGGAAACGt < 2:41536/141‑1 (MQ=255) cTTTTATTAAACATAGATAAAATAAAATGAATGCCACTTTTTAAGAAAACAAAAGCTGAAATACTCATCATTAAAGTAATACTGCCTGCAGGGTTAATTTTCCAAGATAAATAAGATTTCATATTTGTTGCGGAAACGTTc < 1:548438/141‑1 (MQ=255) | CTTTAAGAAATCTTTGGATTTTTGTTTAGTATTGATTAAAAATCTCGATAAAAAATAACCCAATAACATTTGAATCACTAAATATACTGAAATACCTACTGGACTATCAAATGTCAGCATTGGCATGTCATCTGATATGCTTTTATTAAACATAGATAAAATAAAATGAATGCCACTTTTTAAGAAAACAAAAGCTGAAATACTCATCATTAAAGTAATACTGCCTGCAGGGTTAACTTTCCAAGATAAATAAGATTTCATATTTGTTGCGGAAACGTTC > CP000730/2810448‑2810727 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |