Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,064,254 Δ1 bp 100% coding (235/1218 nt) USA300HOU_1000 ← LytR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,064,2530C.95.0% 343.2 / 7.8 78coding (236/1218 nt)USA300HOU_1000LytR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base . (42/32);  total (44/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.52e-01
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GGTAATCTTATCGTAATAGCCAACTTTAGGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCCTTTTTTTCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTAAGTTGGATTGACGATTAGCATTTTTGCTGTCTTGAGAAGATTCGA  >  CP000730/1064114‑1064385
                                                                                                                                           |                                                                                                                                      
ggTAATCTTATCGTAATAGCCAACTTTAGGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTc                                                                                                                                        >  1:236435/1‑140 (MQ=255)
ggTAATCTTATCGTAATAGCCAACTTTAGGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTc                                                                                                                                        >  2:661622/1‑140 (MQ=255)
 gTAATCTTATCGTAATAGCCAACTTTAGGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATGTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTct                                                                                                                                       <  1:252191/140‑2 (MQ=255)
  tAATCTTATCGTAATAGCCAACTTTAGGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTctt                                                                                                                                      <  1:370442/140‑3 (MQ=255)
  tAATCTTATCGTAATAGCCAACTTTAGGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTctt                                                                                                                                      >  1:634880/1‑138 (MQ=255)
    aTCTTATCGTAATAGCCAACTTTATGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCtttt                                                                                                                                    <  1:422511/140‑1 (MQ=255)
            gTAATAGCCAACTTTAGGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCTTTTTttcttct                                                                                                                          <  2:610474/140‑1 (MQ=255)
               aTAGTCAACTTTAGGAATGTAGCTTATAGTATCACGTGGAATACTAAGCATTCTAATTTTATTTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCTTTTTTTCTTCTTcc                                                                                                                       <  1:89759/140‑1 (MQ=255)
                 aGCCAACTTTAGGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTGAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTAAGCATCCTGTCTTTTTTTTTTCTTCCAt                                                                                                                     >  2:720415/1‑140 (MQ=255)
                   ccAACTTTAGGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTATGATCTAGAGTGTTCAGCATCCTGTCTTTTTTTCTTTTTCCTTCg                                                                                                                   >  2:272310/1‑140 (MQ=255)
                    cAACTTTAGGAATGTAGCTGATAGTATCACGTGGAATAATAAGCCTTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTATAATCAAAGCGGCTGATCTAGAGTGTTCAGCATCCTGTCTTTTTTTCTTCTTCCATCGt                                                                                                                  <  1:440553/140‑1 (MQ=255)
                     aaCTTTAGGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCTTTTTTTCTTCTTCCATCGtt                                                                                                                 >  1:740977/1‑140 (MQ=255)
                      aCTTTAGGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAAAGTGTTCAGCATCCTGTCTTTTTTTCTTCTTCCATCGTTa                                                                                                                >  2:686598/1‑140 (MQ=255)
                        tttAGGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTCACTTAGAGTGTTCAGAATACTGTATTTTTTTCTTCTTccgttcttttc                                                                                                              >  1:476066/1‑131 (MQ=255)
                         ttAGGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCTTTTTTTCTTCTTCCATCGTTatca                                                                                                             <  2:598845/140‑1 (MQ=255)
                          tAGGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCTTTTTTTCTTCTTCCATCGTTatcat                                                                                                            >  1:83126/1‑140 (MQ=255)
                            ggAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCTTTTTTTCTTCTTCCATCGTTATCATCg                                                                                                          <  2:475525/140‑1 (MQ=255)
                            ggAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCTTTCTTTCTTCTTCCATCGTTATCATCg                                                                                                          <  1:486027/140‑1 (MQ=255)
                            ggAATGTAGCTGATAGTATCACGTGGAATACTAAGCATACTACTTTGATGTTTTGATTGATTAAAAGTAGTTAAAATAGTAGCGTATGATATCGAGAGTTAAGAATACTGTCTTTTTTTCTTGTTCCATCGTTATCATCg                                                                                                          >  1:463503/1‑140 (MQ=255)
                                 gTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAACAGTAGTTAAAATAATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCTTTTTTTCTTCTTCCTTCGTTATCTTCGATaac                                                                                                     >  2:270181/1‑138 (MQ=255)
                                 gTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCTTTTTTTTTTTTTTTATTGTTATCATCGATccc                                                                                                     >  2:298208/1‑140 (MQ=255)
                                    gCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCTTTTTTTCTTCTTCCATCGTTATCATCGATACCTaa                                                                                                  <  2:710473/140‑1 (MQ=255)
                                      tGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAGAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCTTTTTTTATTCTTCCATCGTTATTAGAGATACCTaaga                                                                                                >  1:99060/1‑140 (MQ=255)
                                       gATAGTATCATGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCTTTTTTTCTTCTTCCATCGTTATCATCGATACCTaagaa                                                                                               <  1:564378/140‑1 (MQ=255)
                                       gATAGTATCAAGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCTTTTTTTCTTCTTCCATCGTTATCATCGATACCTaagaa                                                                                               <  1:356243/140‑1 (MQ=255)
                                        aTAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAAAGAGTTCAGCATCCTGTCTTTTTTTCTTCTTCCATCGTTATCATCGATACCTaataaa                                                                                              >  1:5134/1‑140 (MQ=255)
                                          aGTATCACGTGGAATACTAAGCATGCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCTTTTTTTCTTCTTCCATCGTTATCATCGATACCTaagaaaag                                                                                            <  2:740162/140‑1 (MQ=255)
                                            tATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCTTTTTTTCTTCTTCCATCGTTATCATCGATACCTaagaaaagaa                                                                                          >  1:443784/1‑140 (MQ=255)
                                              tCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCTTTTTTTCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATa                                                                                        >  2:708313/1‑140 (MQ=255)
                                                 cGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCTTTTTTTCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATagag                                                                                     >  2:474484/1‑140 (MQ=255)
                                                  gTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCTTTTTTTCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAgaga                                                                                    <  1:122907/140‑1 (MQ=255)
                                                    ggAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCTTTTTTTCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATa                                                                                  >  1:106762/1‑140 (MQ=255)
                                                     gAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCTTTTTTTCTTCTTCCATCGTTCTCATCGATACCTAAGAAAAGAATAGAGATAg                                                                                 >  2:702735/1‑140 (MQ=255)
                                                       aTACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCTTTTTTTCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGgt                                                                               >  2:109223/1‑140 (MQ=255)
                                                       aTACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCTTTTTTTCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGgt                                                                               >  2:292309/1‑140 (MQ=255)
                                                        tACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGGGGGTTCAGCATCCTGTCTTTTTTTCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGgtt                                                                              <  1:672066/140‑1 (MQ=255)
                                                        tACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCTTTTTTTCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGgtt                                                                              <  1:572123/140‑1 (MQ=255)
                                                        tACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCTTTTTTTCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGgtt                                                                              <  1:572113/140‑1 (MQ=255)
                                                        tACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCTTTTTTTCTTATTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGgtt                                                                              <  1:718083/140‑1 (MQ=255)
                                                          cTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCCTTTTTTTCTTCTTCCATCGTTATCATCGATACCTACGAACAGAATATAGATAGgttg                                                                             >  2:686701/1‑140 (MQ=255)
                                                            aaGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCTTTTTTTCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGttc                                                                          >  1:508517/1‑140 (MQ=255)
                                                            aaGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGAGTCAGATCTAGAGTGTTCAGCATCCTGTCTTTTTTTCTTCTTCCATCGTAATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGttc                                                                          >  1:632460/1‑140 (MQ=255)
                                                                  tCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCTTTTTTTCTTGTTCCATCGTTATCATCGATACCAAAGAAAAGAATAGAGATAGGTTGTTCTTCggg                                                                    >  1:359946/1‑140 (MQ=255)
                                                                  tCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCTTTTTTTCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCggg                                                                    >  1:22599/1‑140 (MQ=255)
                                                                   cTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCTTTTTTTCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGa                                                                   <  2:296485/140‑1 (MQ=255)
                                                                   cTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCTTTTTTTCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAAAGATAGgtttttgcgcttaa                                                                   >  2:530271/1‑132 (MQ=255)
                                                                       tttGATTCTTTAATTGATTAAAGGTATTGAAAATCATAGCGTCTGATCTAGAGTGTTAATTATCCTTTCTTTTTTTCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGa                                                               <  1:203360/140‑1 (MQ=255)
                                                                       tttGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCTTTTTTTCTTCTTCCATCGTTATCATCGATACCTAAAAAAAGAATAGAGATAGGTTGTTCTTCGGGATTgc                                                               >  2:194301/1‑139 (MQ=255)
                                                                        ttGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTCCAGCGTTCAGCATCCTGTCTTTTTTTAACCTAATACTTCG‑‑ACCAGCCATACCTTAGAAAAGAATAGAGATAGGTTGTTCGTCGGGATTGAc                                                              >  2:730790/1‑140 (MQ=255)
                                                                        ttGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCTTTTTTTCTTCTGCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGAc                                                              >  2:544389/1‑140 (MQ=255)
                                                                         tGATGTTTTGATTGTTTAAAAGTAGTTAATATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCTTTTTTTCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACt                                                             <  1:116513/140‑1 (MQ=255)
                                                                         tGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCTTTTTTTCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACt                                                             <  1:570671/140‑1 (MQ=255)
                                                                           aTGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCTTTTTTTCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACttt                                                           >  1:29772/1‑140 (MQ=255)
                                                                            tGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCTTTTTTTCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTtta                                                          <  1:204593/140‑1 (MQ=255)
                                                                               tttGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCTTTTTTTCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTttatta                                                       <  1:756893/140‑1 (MQ=255)
                                                                                 tgattgatTAAAAGTAGTTAAAATCATAGCGGCTGATCTAGAGTGTTCAGCATCCTGTCTTTTTTTCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTAtc                                                     <  2:329459/140‑1 (MQ=255)
                                                                                        ttAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCTTTTTTTCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTAAg                                              <  2:490360/140‑1 (MQ=255)
                                                                                        ctAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCTTTTTTTCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTAAg                                              <  2:644522/139‑1 (MQ=255)
                                                                                             agtagtTAAAATCAGAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCTTTTTTTCTTCTTCCATCGTTATCATCGATACCTAAGAAAAAAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTAAGTTGGa                                         >  1:397199/1‑140 (MQ=255)
                                                                                              gtagtTAATATCATAGCGTCTGATCTAGAGTGTTCCTCATCCTGTTTTTTTTTTTTCTTCCATCGTTATCATGGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTAAGTTGGAt                                        <  1:236001/140‑1 (MQ=255)
                                                                                              gtagtTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCTTTTTTTCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTAAGTTGGAt                                        <  1:696667/140‑1 (MQ=255)
                                                                                                 gtTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCTTTTTTTCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTAAGTTGGATTGa                                     >  1:657638/1‑140 (MQ=255)
                                                                                                  ttAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCTTTTTTTCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTAAGTTGGATTGAc                                    <  2:740977/140‑1 (MQ=255)
                                                                                                     aaaTCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCTTTTTTTTTTTTTTTTTCGTTATCATCGATACCTAAGAAAAGAATAGTGATAGGTTGTTCTTCGGGATTGACTTTATTATCTATTAAGTTGGATAGACGAt                                 >  2:76297/1‑140 (MQ=255)
                                                                                                     aaaTCATAGCGTCTGATCTAGAGTGTTCAGCATACTGTCTTTTTTTTTTCTTTATTTATTATCAACAGTGCATAAGCAAAGAATAGAAATGGTTATTTCTTTGGATTTGACTTTTTTATCGATTAAGTTTGATAGACTAt                                 >  2:230013/1‑140 (MQ=255)
                                                                                                     aaaTCATACCGTCTGAACTAGAGTGTTCAGCATCCTGTCTTTTTTTCTTCTTCGATCGTAATCATCTATACCTAAGAAAAGAATAATGATATGTTGTTGTTCTGGATTGACTTTATTATCTCTTCAGTTGGAttacagat                                 >  2:214983/1‑134 (MQ=255)
                                                                                                      aaTCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCTTTTTTTCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTAAGTTGGATTGACGAtt                                <  1:474484/140‑1 (MQ=255)
                                                                                                      aaTCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCTTTTTTTCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTAAGTTGGATTGACGAtt                                >  1:604453/1‑140 (MQ=255)
                                                                                                       aTCATAGCGTCTGATCTAGATTGTTCAGCATCCTGTCTTTTTTTCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTAAGTTGGATTGACGATTa                               <  2:179090/140‑1 (MQ=255)
                                                                                                        tCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCTTTTTGTCTTCTTCCATCGTTATCATCGATACCCAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTACGTTGGATTGACGATTAg                              >  1:519914/1‑140 (MQ=255)
                                                                                                        ccATAGCGTCTGATCTAGCGTGTTCAGCCTCCTGTGTTTTTTTCTTCTTCCAGCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTAAGTTGGATTGACGATTAg                              <  2:135526/139‑1 (MQ=255)
                                                                                                          aTAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCTTTTTTTTTTTTTTTATCGTTATAATCGGTGCCTAAGAAAAGAATAGAGATAGGTGGTTCTTCGGGGTTGACTTTCTTATCTCTTAAAAAGGATTGCCGAGTAGCa                            >  2:674194/1‑140 (MQ=255)
                                                                                                           tAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCTTTTTTTCTTCTTCCATCGTTATTTTCGTTACTTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTAAGTTGGATTGACGATTAGCAt                           <  1:250173/140‑1 (MQ=255)
                                                                                                           tAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCTTTTTTTCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTAAGTTGGATTGACGATTAGCAt                           >  1:645444/1‑140 (MQ=255)
                                                                                                           tAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCTTTTTTTCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTAAGTTGGATTGACGATTAGCAt                           >  1:447026/1‑140 (MQ=255)
                                                                                                              cGTCTGATCTAGAGTGTTCAGCATCCTGTCTTTTTTTCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTAAGTTGGATTGACGATTAGCAtttt                       <  1:708313/140‑1 (MQ=255)
                                                                                                                tCTGATCTAGAGTGTTCAGCATCCTGTCTTTTTTTCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTAAGTTGGATTGACGATTAGCATTTTTg                     <  1:429597/140‑1 (MQ=255)
                                                                                                                           gtgtTCAGCATCCTGTCTTTTTTTTTTTTTTTTTTTTTATCATCGATACCTAAGAAAAGAATAGAGTTAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTAAGTTGGATTGACGATTAGCATTTTTTCTTTCTTtagt          >  2:289047/1‑139 (MQ=255)
                                                                                                                             gtTCAGCATCCTGTCTTTTTTTCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTAAGTTGGATTGACGATTAGCATTTTTGCTGTCTTGagaag        >  1:627132/1‑140 (MQ=255)
                                                                                                                                cAGCATCCTGTCTTTTTTTCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATAATCTCTTAAGTTGGATTGTCGCATACATCTTTTTCTGTCTTgcacaaatt     >  1:511116/1‑132 (MQ=255)
                                                                                                                                  gCATCCTGTCTTTTTTTCTTCTTCCATCGTTATCATCGATACCTTAGCAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTAAGTTGTATTGTCGATTAGCATTTTTGCTTTCTTGTGAATATTCg   >  2:405094/1‑140 (MQ=255)
                                                                                                                                   cATCCTGTCTTTTTTTCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTAAGTTGGATTGACGATTAGCATTTTTGCTGTCTTGAGAAGATTCGa  >  1:641098/1‑140 (MQ=255)
                                                                                                                                   cATCCTGTCTTTTTTTCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTAAGTTGGATTGACGATTAGCATTTTTGCTGTCTTGAGAAGATTCGa  >  1:539605/1‑140 (MQ=255)
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GGTAATCTTATCGTAATAGCCAACTTTAGGAATGTAGCTGATAGTATCACGTGGAATACTAAGCATTCTAATTTGATGTTTTGATTGATTAAAAGTAGTTAAAATCATAGCGTCTGATCTAGAGTGTTCAGCATCCTGTCCTTTTTTTCTTCTTCCATCGTTATCATCGATACCTAAGAAAAGAATAGAGATAGGTTGTTCTTCGGGATTGACTTTATTATCTCTTAAGTTGGATTGACGATTAGCATTTTTGCTGTCTTGAGAAGATTCGA  >  CP000730/1064114‑1064385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: