Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 39,378 C→A 100% D586Y (GAT→TAT)  mecA ← penicillin binding protein 2 prime

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073039,3780CA100.0% 165.9 / NA 55D586Y (GAT→TAT) mecApenicillin binding protein 2 prime
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (29/26);  total (29/26)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATCTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTTAATAAGTGAGGTGCGTTAATATTGCCA  >  CP000730/39254‑39511
                                                                                                                            |                                                                                                                                     
atGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGtttt                                                                                                                        >  1:321428/1‑140 (MQ=255)
  gTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTtttat                                                                                                                      >  2:269898/1‑140 (MQ=255)
      ggATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTAc                                                                                                                  <  1:212298/140‑1 (MQ=255)
              tttATCATATTGTATAAACCACCCAATTTGTCTGCCATTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATCTACGCTTTATGTGTTTTATTTACGACttgtt                                                                                                          <  2:733596/140‑1 (MQ=255)
              tttACCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACttgtt                                                                                                          <  2:551936/140‑1 (MQ=255)
                  tcagtggATATAAACCACCCAATGTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCTATTAATTTTTCATAAGATCTATAATTATATTCTTTATGTGTTTTATTTACGACTTGTTGCAt                                                                                                      <  2:22414/134‑1 (MQ=255)
                  tCATATGTTATAAACCACCCACTTTGTCTGCCATTTTCTCCTTGTTTCATTGTGAGTTCTGCAGTAACGGTTTTGCCACTTAAGGCTGCATAAGATCTATAAATATATTCTTGATGTGTTTTATTTACGACTTGTTGCAt                                                                                                      <  2:23083/140‑1 (MQ=255)
                    atatGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCTTAc                                                                                                    >  1:333305/1‑140 (MQ=255)
                    atatGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATAc                                                                                                    >  1:414253/1‑140 (MQ=255)
                       tGATATAAGCCACCCAATTTGTCTGCCAGTTGCTGCTTGGTACATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAACTATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCAt                                                                                                 <  2:728378/140‑1 (MQ=255)
                       cgctataAACCACCCAGGTGGACGGCCGTGTTCGCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATAGAGTCTTTATGTGTTTTATTTACGACTTGTTGCATACCAt                                                                                                 <  2:530614/137‑1 (MQ=255)
                          tataAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTAAAATCAGCAGCACCAAAAAAACCAATTGAGTTTGGATAAGAACTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCat                                                                                              >  1:835/1‑139 (MQ=255)
                           ataAACCACCCATTTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCAAAAGATCTATAAATATATTCTTTATTTGTTTTATTTACGACTTGTTGCATACCATCAGt                                                                                             <  1:719269/140‑1 (MQ=255)
                                    ccAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGAtt                                                                                    >  1:14923/1‑140 (MQ=255)
                                     cAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTg                                                                                   >  1:405245/1‑140 (MQ=255)
                                      aaTTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAACTATATTCTTTATGTGTTTTATTTACGGCTTGTTGCATACCATCCGTTAATAGATTgc                                                                                  >  1:147866/1‑139 (MQ=255)
                                      aaTTTGTCTGCCAGTTGCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGa                                                                                  <  2:553326/140‑1 (MQ=255)
                                      aaTTTGTCTGCCAGGTGCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGa                                                                                  <  2:687619/140‑1 (MQ=255)
                                       aTTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGTCTTGTTGCATACCATCAGTTAATAGATTGat                                                                                 >  1:89118/1‑140 (MQ=255)
                                       aTTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGat                                                                                 >  1:227133/1‑140 (MQ=255)
                                       aTTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGat                                                                                 >  1:371288/1‑140 (MQ=255)
                                       aTTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTACATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGat                                                                                 >  1:9017/1‑140 (MQ=255)
                                          tGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATAtt                                                                              <  1:497471/140‑1 (MQ=255)
                                          ggtttgtgctgttCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAATATCTAAAAATATATTCTTTAGGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATAtt                                                                              <  2:218682/129‑1 (MQ=255)
                                            tCTGCCAGTTTCTCCTTGTTTCTTTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTAAGACTTGTTGCATACCATCAGTTAATAGATTGATAtttt                                                                            <  2:226129/140‑1 (MQ=255)
                                            tCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATAtttt                                                                            <  1:352068/140‑1 (MQ=255)
                                            tCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATAtttt                                                                            <  1:238455/140‑1 (MQ=255)
                                            tCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATAtttt                                                                            <  1:658054/140‑1 (MQ=255)
                                                 cAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTg                                                                       >  1:545670/1‑140 (MQ=255)
                                                    tttCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGaa                                                                    >  2:200837/1‑140 (MQ=255)
                                                     ttCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGaaa                                                                   >  1:681359/1‑140 (MQ=255)
                                                                   ttttGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTttcttc                                                     >  1:301885/1‑140 (MQ=255)
                                                                    tttGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTcc                                                    <  1:686571/140‑1 (MQ=255)
                                                                         tttCTGCAGTACCGGATTTGCCAATTTAGTTTGCATATGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACt                                               <  1:650165/139‑1 (MQ=255)
                                                                          ttCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACtt                                              >  1:655507/1‑140 (MQ=255)
                                                                          ttCGGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATTTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACtt                                              <  1:268345/140‑1 (MQ=255)
                                                                           tCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACttt                                             <  1:269898/140‑1 (MQ=255)
                                                                           tCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACttt                                             >  2:335014/1‑140 (MQ=255)
                                                                             tGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGt                                           <  1:534915/140‑1 (MQ=255)
                                                                               cAGTACCGGATTTTCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATGGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGttt                                         <  2:487157/140‑1 (MQ=255)
                                                                                  tACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTc                                      <  2:676387/140‑1 (MQ=255)
                                                                                           tGCCAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCtttt                             >  2:545955/1‑140 (MQ=255)
                                                                                              cAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTtaat                          >  1:685800/1‑140 (MQ=255)
                                                                                              cAATTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTtaat                          >  1:190112/1‑140 (MQ=255)
                                                                                               aaTTAAGTTTGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTtaata                         >  1:471331/1‑140 (MQ=255)
                                                                                                aTTAAGTTTGCATAAGATTTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTtaataa                        <  1:314026/140‑1 (MQ=255)
                                                                                                      tttGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTtaat                          <  1:238981/132‑1 (MQ=255)
                                                                                                      tttGCATAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTtaat                          >  2:238981/1‑132 (MQ=255)
                                                                                                            tAAGATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTTAATAAGTGAGGTGCGtt            >  2:137451/1‑140 (MQ=255)
                                                                                                               gATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCTTTTTTTTTAATAAGTAAGGTGCGTTCat         >  2:592060/1‑140 (MQ=255)
                                                                                                               gATCTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTGGTTTTTCGTGTCTTTTAATAAGTGAGTTGCGTTAat         >  2:510211/1‑140 (MQ=255)
                                                                                                               gATCTATAAATATATTCTTTATGTGTATTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTGTTTCGTGTCTTTTAATAAGTGAGGTGCGTTAat         >  2:472632/1‑140 (MQ=255)
                                                                                                                  cTATAAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTTAATAAGTGAGGTGCGTTAATAtt      >  2:591690/1‑140 (MQ=255)
                                                                                                                     taAATATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCCAACTTTGTTTTTCGTGTCTTTTAATAAGTGAGGTGCGTTAATATTGcc   <  2:671311/140‑1 (MQ=255)
                                                                                                                      aaaTATATTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTTAATAAGTGAGGTGCGTTAATATTGCCa  >  1:525077/1‑140 (MQ=255)
                                                                                                                            |                                                                                                                                     
ATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATCTTCTTTATGTGTTTTATTTACGACTTGTTGCATACCATCAGTTAATAGATTGATATTTTCTTTGGAAATAATATTTTTCTTCCAAACTTTGTTTTTCGTGTCTTTTAATAAGTGAGGTGCGTTAATATTGCCA  >  CP000730/39254‑39511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: