Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,289,262 C→G 100% W76C (TGG→TGC USA300HOU_2153 ← hypothetical membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,289,2620CG96.2% 186.1 / ‑1.9 68W76C (TGG→TGCUSA300HOU_2153hypothetical membrane protein
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base G (24/39);  total (28/40)
Fisher's exact test for biased strand distribution p-value = 5.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GATTGAATCAATTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGA  >  CP000730/2289131‑2289397
                                                                                                                                   |                                                                                                                                       
gATTGAATCAATTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAAt                                                                                                                                 >  2:666229/1‑140 (MQ=255)
gATTGAATCAATTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACACCGCATTGAAt                                                                                                                                 >  2:360334/1‑140 (MQ=255)
gATTGAATCAATTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTAAGTCGACCAAGGTGTTCTAAGGCACGTCTAAATTCTGGTAGAAATATTCCTACCAAAGCTACTACCCCGCATTGAAt                                                                                                                                 <  1:159031/140‑1 (MQ=255)
      aTCAATTTTTCTTCATCTTTACTAAACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGat                                                                                                                           <  2:726559/140‑1 (MQ=255)
          aTTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCg                                                                                                                       <  2:13093/140‑1 (MQ=255)
          aTTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCg                                                                                                                       <  2:447336/140‑1 (MQ=255)
           tttCTTTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGa                                                                                                                      <  2:320188/140‑1 (MQ=255)
              ctctTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATCCCCCCCATTGAATAACGATATCGaata                                                                                                                   >  2:432616/1‑140 (MQ=255)
               tctTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTGGTTCTAAAGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGTTCTAGaataa                                                                                                                  >  2:95413/1‑140 (MQ=255)
                 ttCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAAGGATATCGAATAAtt                                                                                                                <  2:517297/140‑1 (MQ=255)
                          aCTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTg                                                                                                       >  1:508017/1‑140 (MQ=255)
                             cgaCGTATTAGAAGTATAGCCTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAg                                                                                                    <  2:228331/138‑1 (MQ=255)
                              taCGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTGCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTTCAGt                                                                                                   <  2:461954/140‑1 (MQ=255)
                                   aTTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTATAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGt                                                                                              <  1:52116/140‑1 (MQ=255)
                                   aTTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGt                                                                                              >  2:398260/1‑140 (MQ=255)
                                   aTTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGt                                                                                              <  1:64682/140‑1 (MQ=255)
                                     tAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACTCACGTCTAATTTCTGGTTGGAATATTACTTTTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTc                                                                                            <  1:441899/140‑1 (MQ=255)
                                      aGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCa                                                                                           <  2:241227/140‑1 (MQ=255)
                                        caGTATAGGGTTTGAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTTCAGTCAAGTTCAat                                                                                         <  2:578625/139‑1 (MQ=255)
                                        acctataGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTTTTAAAGCTAATACCCCGCATTGAATAACGATAACGAATAATTTAGATGTTGCAGTCAAGTTCCat                                                                                         >  2:655437/4‑140 (MQ=255)
                                        aaGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTTAATAACGATATCGAATAATTTACATGGAGCCGTCAAGGTCaac                                                                                         >  2:38463/1‑139 (MQ=255)
                                           tataGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATc                                                                                      >  2:251499/1‑140 (MQ=255)
                                             taGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAGTAATATAGATGTTGCTGTTCAGCTCAATATAAt                                                                                    >  2:63363/1‑140 (MQ=255)
                                             taGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCAt                                                                                    >  1:9570/1‑140 (MQ=255)
                                              aGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATa                                                                                   <  2:596730/140‑1 (MQ=255)
                                               gCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAACTTTTTCCCCTCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATAc                                                                                  >  1:748362/1‑140 (MQ=255)
                                                 gTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACtt                                                                                >  1:312608/1‑140 (MQ=255)
                                                 cttttAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCAGCATTGAATACCGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACtt                                                                                <  2:610102/139‑1 (MQ=255)
                                                   tttAAAAAGCTCCCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTAt                                                                              <  2:712897/140‑1 (MQ=255)
                                                   tttAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTAt                                                                              <  2:215387/140‑1 (MQ=255)
                                                   tttAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTAt                                                                              <  2:194624/140‑1 (MQ=255)
                                                   tttAAAAAGCTACCTCGTCCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGCATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTAt                                                                              <  2:252683/140‑1 (MQ=255)
                                                     tAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATct                                                                            <  1:296829/140‑1 (MQ=255)
                                                     tAAAAAGCTACCTCTACCAAGTTGTTATAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGGTGCAGTCAAGTTCAATATCATACTTATct                                                                            <  1:350919/140‑1 (MQ=255)
                                                      aaaaaGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTTGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATAGAATAATTTAGATGTTGCAGTAAAGTTCAATATCATACTTAtttt                                                                           >  2:343024/1‑137 (MQ=255)
                                                        aaaGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATctgct                                                                         <  1:629742/140‑1 (MQ=255)
                                                        aaaGCTACCTCTACCAAGTTGGTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTAGTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATctgct                                                                         <  1:173528/140‑1 (MQ=255)
                                                           gCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGAc                                                                      <  2:502581/140‑1 (MQ=255)
                                                               ccTCTACCAAGTTTTTCTAACGTACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAAt                                                                  <  1:509479/140‑1 (MQ=255)
                                                               ccTCTACCAAGTTGTTCTAACGCACGTTTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAAt                                                                  <  1:286654/140‑1 (MQ=255)
                                                               ccTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAAt                                                                  <  1:422190/140‑1 (MQ=255)
                                                                 tctACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATaa                                                                >  2:135109/1‑140 (MQ=255)
                                                                        aGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAAATGTTTCAGTCAACTTCAATATCTTACTAATGTGCTTACCAATAACAATTAc                                                         >  1:287137/1‑140 (MQ=255)
                                                                         gttgttCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACt                                                        <  1:583171/140‑1 (MQ=255)
                                                                         gttgttCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACt                                                        <  1:631019/140‑1 (MQ=255)
                                                                         gtgcttCTAACGCACGGCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATCATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACt                                                        <  2:509167/136‑1 (MQ=255)
                                                                          ttgttCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTATATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACCATTACTa                                                       <  1:680562/140‑1 (MQ=255)
                                                                          ttgttCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTa                                                       <  1:500025/140‑1 (MQ=255)
                                                                             ttCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTACTGACCAATAACAATTACTAata                                                    >  1:37896/1‑140 (MQ=255)
                                                                               cTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACCATTACTAATATc                                                  >  2:384920/1‑140 (MQ=255)
                                                                               cTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAataac                                                  >  1:453027/1‑138 (MQ=255)
                                                                               cTAACGCACGACTAATTTCTGGTTGGAATATTACTAGTAACGGTCATACCCCATATGTAATAAGGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATc                                                  >  2:159797/1‑140 (MQ=255)
                                                                                 aaCGCACGTCTAACTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACttaattccc                                                <  2:658222/140‑9 (MQ=255)
                                                                                   cGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCtt                                              >  2:655195/1‑140 (MQ=255)
                                                                                   cGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACAAAATCAAATAATTTAGATGTAGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCACtt                                              >  1:200919/1‑140 (MQ=255)
                                                                                     cACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTTTCTGCTGACCAATAACCATTACTAATATcccgtaa                                            >  2:446043/1‑136 (MQ=255)
                                                                                          cTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTaa                                       >  2:366062/1‑140 (MQ=255)
                                                                                           tAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAAt                                      >  2:220502/1‑140 (MQ=255)
                                                                                           tAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAAt                                      >  2:663006/1‑140 (MQ=255)
                                                                                                 cTGGTTGGAATATTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATc                                >  1:434186/1‑140 (MQ=255)
                                                                                                      tGGAAGATTACTATTAAAGCTAATACCCCGCATTGAATAACGTGATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCttt                           <  2:170958/140‑1 (MQ=255)
                                                                                                           tatTACTATTAATGCTCATACCCCGCTTTGAATAACGATATCGAATATTTTAGATGTTGCAGTCTAGTTCTATTTCATTCTTTTCTGCTGATCAATATCTATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTc                      <  1:27236/140‑1 (MQ=255)
                                                                                                            atTACTATTAAAGCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTcc                     <  1:398260/140‑1 (MQ=255)
                                                                                                                        gCTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGt         <  1:382917/140‑1 (MQ=255)
                                                                                                                         cTAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGtg        <  1:350214/140‑1 (MQ=255)
                                                                                                                          tAATACCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGtga       >  2:634694/1‑140 (MQ=255)
                                                                                                                          gaaTACCCCGCATTGAATAACGATATCGAATAATTTAGGTGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGtga       <  2:412603/139‑1 (MQ=255)
                                                                                                                              aCCCCGCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATgag   <  1:263049/140‑1 (MQ=255)
                                                                                                                               ccccgcATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATgaga  <  1:536576/140‑1 (MQ=255)
                                                                                                                               ccccgcATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATgaga  <  2:434186/140‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                       
GATTGAATCAATTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCGTGATGAGA  >  CP000730/2289131‑2289397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: