Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,532,298 A→T 100% T15T (ACA→ACT USA300HOU_2392 → FemAB family peptidoglycan biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,532,2980AT100.0% 247.0 / NA 76T15T (ACA→ACTUSA300HOU_2392FemAB family peptidoglycan biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (25/51);  total (25/51)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAGCAC  >  CP000730/2532166‑2532434
                                                                                                                                    |                                                                                                                                        
aTTTTTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGc                                                                                                                                   <  1:760841/140‑1 (MQ=255)
aTTTGGAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGc                                                                                                                                   <  2:534288/140‑1 (MQ=255)
      aaTCATTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATGATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCAttttt                                                                                                                             <  1:326861/140‑1 (MQ=255)
      aaTCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCAttttt                                                                                                                             <  2:736891/140‑1 (MQ=255)
         cAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTcac                                                                                                                          <  1:344710/140‑1 (MQ=255)
         cAGTTTGTGATAAAATAAAAAAATAGCATTATTAATAAATTTAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAAGGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTcac                                                                                                                          <  1:78784/140‑1 (MQ=255)
           gTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCACAGCTTGTAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACAt                                                                                                                        >  1:400719/1‑140 (MQ=255)
              tttGATACAATTATGAGATAGCGTGACTAATAACTTACAACCTGATAGCTCCATTCAGAGCGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTac                                                                                                                     <  1:343694/140‑1 (MQ=255)
                tGATAAAATAAAAAAATAGCATTTTTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTacac                                                                                                                   >  2:195333/1‑140 (MQ=255)
                tGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACACAGCATTTTTCACATTacac                                                                                                                   <  1:402655/140‑1 (MQ=255)
                  ataaaaaaaaAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTacacac                                                                                                                 <  2:325925/140‑1 (MQ=255)
                   taaaataaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATACAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACATAGCATTTTTCACATTAcacaca                                                                                                                <  2:518518/140‑1 (MQ=255)
                     aaataaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTCTATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACGTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAAt                                                                                                              <  1:604719/140‑1 (MQ=255)
                     aaataaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCTCATTACACaaaag                                                                                                              >  2:442180/1‑139 (MQ=255)
                      aataaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAAGTTAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATc                                                                                                             <  2:609718/140‑1 (MQ=255)
                        taaaaACATTGCGTTATTTATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAATATGTTTTTTGTATCGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAg                                                                                                           <  1:71721/140‑1 (MQ=255)
                          aaaaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAACGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCt                                                                                                         <  2:679447/140‑1 (MQ=255)
                           aaaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCGTTTTTCACATTACACACAATCGGCTa                                                                                                        >  2:447996/1‑140 (MQ=255)
                            aaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGCGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTAt                                                                                                       >  1:429303/1‑140 (MQ=255)
                            aaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTAt                                                                                                       <  2:220283/140‑1 (MQ=255)
                             aaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGAATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTATCACATTACACACAATCAGCTAtt                                                                                                      >  2:249533/1‑140 (MQ=255)
                              aaTAGCATTATTAATAAATTCAAAAATTATAGTTCACTGAATATAGAGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTc                                                                                                     <  1:709783/140‑1 (MQ=255)
                               aTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCa                                                                                                    <  2:27767/140‑1 (MQ=255)
                               aTAGCATTAATAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCa                                                                                                    <  2:689046/140‑1 (MQ=255)
                                tAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGGCTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCAt                                                                                                   <  1:680941/140‑1 (MQ=255)
                                 aacaataataaaaaATTCAAAAATTATAGTTCAAATCAGAGAGGGAAGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCAtt                                                                                                  <  1:158647/132‑1 (MQ=255)
                                   cATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTAc                                                                                                <  1:185933/140‑1 (MQ=255)
                                             aaaTTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATagag                                                                                      <  2:493685/140‑1 (MQ=255)
                                               aTTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGtt                                                                                    <  2:680522/140‑1 (MQ=255)
                                                 tCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGa                                                                                  <  1:54336/140‑1 (MQ=255)
                                                    aaaaTTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGAttt                                                                               >  2:185083/1‑140 (MQ=255)
                                                             gTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGa                                                                      >  1:268699/1‑140 (MQ=255)
                                                             gTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGAATAAGGATAAGCTCAATGCGATTACTACAAAGCAATCTTCACATCAACCACAATCAGCCACTCATGACAAACAAAGAGTTGATTTAAAAGGCGa                                                                      >  2:65642/1‑140 (MQ=255)
                                                               tCAATTCAGAGCGGGATGGGATAAAATGGACCTTGTAACGTTGACTTCGGATGAGGTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGGTGATTTAAAAGGCGAtg                                                                    <  2:86167/140‑1 (MQ=255)
                                                               tCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGAtg                                                                    <  2:566821/140‑1 (MQ=255)
                                                                  atgtaggcggggatgggatAAAATGATCTTTGTAACGTTGATTTCGGATGGGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGc                                                                 <  2:400719/131‑1 (MQ=255)
                                                                   ttCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCa                                                                >  2:737844/1‑140 (MQ=255)
                                                                     cAGAGAGGGATGGGATAATATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATc                                                              <  2:600435/140‑1 (MQ=255)
                                                                     cAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAGAGGGGATGTGCATc                                                              <  2:514923/140‑1 (MQ=255)
                                                                     cAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATc                                                              <  2:564282/140‑1 (MQ=255)
                                                                      agagagagATGGGATAAAACGAACTTTGTAACGTTGACTTCGGATGAGTTCACTGCGTTTCCTACAAAGCCTTTTTCACCTTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCt                                                             <  2:175001/140‑1 (MQ=255)
                                                                       gagagGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATATGCATCtt                                                            <  1:513614/140‑1 (MQ=255)
                                                                        agagGTATGGGATACAATTCACTTTGTAACGTTTACTTCGGATGAGTTTAATGCGTTTACTACAAAGCATTTTTCCCATTACACACCATCAGCTATTCATTACTATCATAGAGTTGATTTAATAGGCGATGTGCATCTTg                                                           <  2:626725/140‑1 (MQ=255)
                                                                             gatgggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTAACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGAAGTGCGTCTTGGAggg                                                      >  1:51003/1‑140 (MQ=255)
                                                                              atgggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCaaccgggaaggt                                                     >  2:530262/1‑129 (MQ=255)
                                                                               tggggtAAGATGGTCTGTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTATAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGt                                                    <  2:405861/140‑1 (MQ=255)
                                                                               tgggatAAAATTAACTTGTTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGt                                                    <  1:449546/140‑1 (MQ=255)
                                                                               tgggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGt                                                    <  1:281131/140‑1 (MQ=255)
                                                                               tgggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTAAAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGt                                                    <  2:405858/140‑1 (MQ=255)
                                                                               tgggatAAAATGAACTTTGTAACGTTGACTTCCGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGt                                                    <  1:584962/140‑1 (MQ=255)
                                                                               tgggatAAAATGAACTTTGGAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAAGCAGCTATTCATTACAATCACAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGt                                                    <  1:86135/140‑1 (MQ=255)
                                                                                 ggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGAGCATCTTGTAGGGATTa                                                  >  2:400040/1‑140 (MQ=255)
                                                                                  gatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTaa                                                 <  1:101117/140‑1 (MQ=255)
                                                                                      aaaTGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGAt                                             <  1:752959/140‑1 (MQ=255)
                                                                                      aaaTGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGAt                                             <  2:634803/140‑1 (MQ=255)
                                                                                      aaaTGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTATACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATCGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGAt                                             <  1:334448/140‑1 (MQ=255)
                                                                                       aaTGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACCTTACACACAATCGGCTATTCATTACAAGCATTGATTTAAGTTAAAATGCGATGGGCATCTTGTAGGGGTTAAAGATg                                            <  2:28406/140‑1 (MQ=255)
                                                                                            aCTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAAt                                       >  1:536597/1‑140 (MQ=255)
                                                                                                 gTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGTATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCa                                  <  1:322512/140‑1 (MQ=255)
                                                                                                       ttGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGAt                            <  1:675474/140‑1 (MQ=255)
                                                                                                        tGACGTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGAtt                           <  2:656424/140‑1 (MQ=255)
                                                                                                        ggACTTCGGATGAGTTCAATGCGTTTACTACAAATCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGAtt                           <  2:54398/139‑1 (MQ=255)
                                                                                                           cTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCa                        >  2:545296/1‑140 (MQ=255)
                                                                                                            ttCGGATGAGTTCAATGCGTTTACTACAAAGTATTTTTCACATTACACACAATCAGTTATTAATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAg                       <  1:287993/140‑1 (MQ=255)
                                                                                                              cGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATACAAAACCCGACGGTCATCTTATAGGGGTTAAAGATGACAATGGTCAAATGATTGCAGGa                     >  1:712966/1‑140 (MQ=255)
                                                                                                               ggttGAGTTCAACGCGTTTACTACAAAGCATTTTTCACATTACACAAAACCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGTGGTTAAAGACGACAATGGTCAAGTGATTGCAGGAt                    <  2:604127/137‑1 (MQ=255)
                                                                                                                gATGAGTTCAATGCGTTTAGTACAAGGCATTTTTCACATTACACGCAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGTGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATg                   >  1:416956/1‑140 (MQ=255)
                                                                                                                gATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATg                   >  2:638082/1‑140 (MQ=255)
                                                                                                                gATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTAAACTCATAGAGTTGATTTAAAAGGCGATGTGCAACTTGTAGGGGTCAAAGATGACAATGGTCAAGTGATTGCAGGATg                   >  2:184899/1‑140 (MQ=255)
                                                                                                                   gAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCtt                >  1:162691/1‑140 (MQ=255)
                                                                                                                     gTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTAt              >  1:190783/1‑140 (MQ=255)
                                                                                                                         aaTGCGTTTACTACAAAGCATTTTTCACATTACATCAAAACAGTTATGCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGAc          >  2:312204/1‑140 (MQ=255)
                                                                                                                         aaTGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCAATGAGCATCTTGTAGGGGTTAAAGATGACAATGGTAAAGTGATTGCAGGCAACTAATTGAc          >  1:179629/1‑140 (MQ=255)
                                                                                                                         aaTGCGTTTACTACAAAGAAGCTTTCACATAACACACAATCAGCTATACATTACAAAAATAGAGATGATTTAAAAGGCGATGTGCGCCTTATAGGGGGGAAAGATGACAATGGTCAAGTGATTGAAGGCTGGTTATGGAc          >  2:325419/1‑140 (MQ=255)
                                                                                                                          aTGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGAAAATAGAGATATGATGGCAGGATGCTTATTGACa         <  1:754976/140‑1 (MQ=255)
                                                                                                                              gTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAGGGCGATGTGCATCTTGTAGTGGCTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAg     >  2:730079/1‑140 (MQ=255)
                                                                                                                                 tactacAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATTTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACATAAgcac  <  2:276045/140‑1 (MQ=255)
                                                                                                                                 tactacAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAgcac  <  1:661769/140‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                        
ATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAGCAC  >  CP000730/2532166‑2532434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: