Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,614,662 A→G 7.8% I3I (ATT→ATC)  USA300HOU_2478 ← lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,614,6620AG 7.8% 177.8 / 4.0 70I3I (ATT→ATC) USA300HOU_2478lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (43/19);  new base G (3/3);  total (46/24)
Fisher's exact test for biased strand distribution p-value = 3.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

TGGATACAGGCTCAACGTTTTGTTGAAGTTTTCTTTAATTTGTTTATCTTTACTGCTTTCTTCCTTTTTCATTCCGCAAGCCCCTATAAAAACAGCTAATAATACTAAATACAGCCAGAGCCTCAACCTTTTTGAGTGAATCATCATTTCACATCCCCATTTTTATTTTTGATGCAAAGTTAAGTTCAAATTCCAATATTAATAATATTAGAGGAAATTTTATGATTAAATCCTCGTGGATTTTAATTGTTAGTTGTATTTTTCACATATCAACAT  >  CP000730/2614524‑2614799
                                                                                                                                          |                                                                                                                                         
tGGATACAGGCTCAACGTTTTGTTGAAGTTTTCTTTAATTTGTTTATCTTTACTGCTTTCTTCCTTTTTCATTCCGCAAGCCCCTATAAAAACAGCTAATAATACTAAATACAGCCAGAGCCTCAACCTTTTTGAGTGaa                                                                                                                                          <  1:594741/140‑1 (MQ=255)
tGGATACAGGCTCAACGTTTTGTTGAAGTTTTCTTTAATTTGTTTATCTTTACTGCTTTCTTCCTTTTTCATTCCGCAAGCCCCTATAAAAACAGCTAATAATACTAAATACAGCCAGAGCCTCAACCTTTTTGAGTGaa                                                                                                                                          <  1:594731/140‑1 (MQ=255)
tGGATACAGGCTCAACGTTTTGTTGAAGTTTTCTTTAATTTGTTTATCTTTACTGCTTTCTTCCTTTTTCATTCCGCAAGCCCCTATAAAAACAGCTAATAATACTAAATACAGCCAGAGCCTCAACCTTTTTGAGTGaa                                                                                                                                          <  1:272077/140‑1 (MQ=255)
 ggttACAGGCTCAACGTTTTGTTGAAGTTTTCTTTAATTTGTTTATCTTTACTGCTTTCTTCCTTTTTCATTCCGCAAGCCCCTATAAAAACAGCTAATAATACTAAATACAGCCAGAGCCTCAACCTTTTTGAGTGAat                                                                                                                                         <  2:75441/137‑1 (MQ=255)
        ggCTCAACGTTTTGTTGAAGTTTTCTTTAATTTGTTTATCTTTACTGCTTTCTTCCTTTTTCATTCCGCAAGCCCCTATAAAAACAGCTAATAATACTAAATACAGCCAGAGCCTCAACCTTTTTGAGTGAATCATCAtt                                                                                                                                  >  1:292974/1‑140 (MQ=255)
        ggCTCAACGTTTTGTTGAAGGTTTCTTTAATTTGTTTATCTTTATTGCTTTCTTCCTTTTTCATTCCGCAAGCCCCTATAAAAACGGGTAATAATACTAAATACAGCCAGAGCCTCAACCTTTTTGAGTGAATCATCAtt                                                                                                                                  >  2:416931/1‑140 (MQ=255)
            cAACGTTTTGTTGAAGTTTTCTTTAATTTGTTTATCTTTACTGCTTTCTTCCTTTTTCATTCCGCAAGCCCCTATAAAAACAGCTAATAATACTAAATACAGCCAGAGCCTCAACCTTTTTGAGTGAATCATCATTTcac                                                                                                                              >  1:652952/1‑140 (MQ=255)
            cAACGTTTTGTTGAAGTTTTCTTTAATTTGTTTATCTTTACTGCTTTCTTCCTTTTTCATTCCGCAAGCCCCTATAAAAACAGCTAATAATACTAAATACAGCCAGAGCCTCAACCTCTTTAAGTGAATCATCATTTcac                                                                                                                              >  1:633434/1‑140 (MQ=255)
               cggtttGTTGAAGTTTTCTTTAATTTGTTTATCTTTACTGCTTTCTTCCTTTTTCATTCCGCAAGCCCCTATAAAAACAGCTAATAATACTAAATACAGCCAGAGCCTCAACCTTTTTGAGTGGATCATCATTTCACATc                                                                                                                           >  2:203561/4‑140 (MQ=255)
               cGTTTTGTTGAAGTTTTCTTTAATTTGTTTATCTTTACTGCTTTCTTCCTTTTTCATTCCGCAAGCCCCTATAAAAACAGCTAATAATACTTAATACAACCAGAGCCCCAACCTATTTGAGTGAATCATCATTTCACATc                                                                                                                           >  1:127068/1‑140 (MQ=255)
               cGTTTTGTTGAAGTTTTCTTTAATTTGTTTATCTTTACTGCTTTCTTCCTTTTTCATTCCGCAAGCCCCTATAAAAACAGCTAATAATACTAAATACAGCCAGAGCCTCAACCTTTTTGAGTGAATCATCATTTCACATc                                                                                                                           >  2:578888/1‑140 (MQ=255)
                gTTTTGTTGAAGTCTTCTTTAATTTGTTTATCTTTACTGCTTTCTTCCTTTTTCATTCCGCAAGCCCCTATAAAAACAGCTAATAATACTAAATACAGCCAGAGCCTCAACCTTTTTGAGTGAATCAACATTTCACATcc                                                                                                                          >  2:179752/1‑140 (MQ=255)
                gTTTAGTTGAAGTTTTCTTTAATTTGTTTATCTTTACTGCTTTCTTCCTTTTTCATTCCGCAAGCCCCTATAAAAACAGCTAATAATACTAAATACAGCCAAAGCCTCAACCTTTTTGAGTGAATCATCATTTCACATcc                                                                                                                          >  2:461674/1‑140 (MQ=255)
                 ttttGTTGAAGTTTTCTTTAATTTGTTTATCTTTACTGCTTTCTTCCTTTTTCATTCCGCAAGCCCCTATAAAAACAGCTAATAATACTAAATACAGCCAGAGCCTCAACCTTTTTGAGTGAATCATCATTTCACATccc                                                                                                                         >  2:411011/1‑140 (MQ=255)
                  tttGTTGAAGTTTTCTTTAATTTGTTTATCTTTACTGCTTTCTTCCTTTTTCATTCCGCAAGCCCCTATAAAAACAGCTAATAATACTAAATACAGCCAGAGCCTCAACCTTTTTGAGTGGATCATCATTTCACATcccc                                                                                                                        >  1:445533/1‑140 (MQ=255)
                  tttGTTGAAGTTTTCTTTAATTTGATTATCTTTACTGCTTTCTTCCTTTTTCCTTCCGCAAGCCGCTATTGGAACAGCTAATAATACTAAATACAGCCAGAGCCTCAACCTTTTTGAGTGGATCATCATTTCACATcccc                                                                                                                        >  1:556207/1‑140 (MQ=255)
                               tCTTTAATTTGTTTATCTGTACTGCTTTCTTCCTTTTTCATTCCGCAAGCCCCTATAAAAACAGCTAATAATACTAAATACAGCCAGAGCCTCAACCTTTTTGAATGAATCATCATTTCACATCCCCATTTTTATTTTTg                                                                                                           >  2:368010/1‑140 (MQ=255)
                                  ttAATTTGTTTATCTTTACTGCTTTCTTCCTTTTTCATTCCGCAAGCCCCTATAAAAACAGCTAATAATACTAAATACAGCCAGAGCCTCAACCTTTTTGAGTGAATCCTCATTTCACATCCCCATTTTTATTTTTGATg                                                                                                        >  2:365819/1‑140 (MQ=255)
                                          tttATCTTTACTGCTTTCTTCCTTTTTCATTCCGCAAGCCCCTATAAAAACAGCTAATAATACTAAATACAGCCAGAGCCTCAACCTTTTTGAGTGAATCATCATTTCACATCCCCATTTTTATTTTTGATGCAaagtta                                                                                                >  1:701197/1‑140 (MQ=255)
                                          tttATCTTTACTGCATTCTACCTTTTTCATTCCGCACGACCCTATAAAAACAGCTAATAATTCTAAATCCAGCCAGAGCCTCAACCTTTTTGAGTGAATAATAAATTCCTTTCCCCATTTTTATTTTTGAtaatacgtta                                                                                                >  2:511422/1‑131 (MQ=255)
                                            tATCTTTACTGCTTTCTTCCTTTTTCATTCCGCAAGCCCCTATAAAAACAGCTAATAATACTAAATACAGCCAGAGCCTCAACCATTTTAAGTGAATCATCATTTCACATCCCCATTTTTATTTTTGATGCAaagttaag                                                                                              >  1:422735/1‑140 (MQ=255)
                                               cTTTACTGCTTTCTTCCTTTTTCATTCTTCAAGCCCCTATAAAAACAGCTAAAAATAATAAATACAGCCAGAGCCTCAACCTTTTTTAGTGAATCATCATTTCACATTCGATTTTATATTTTTTAttaataatttagttg                                                                                           >  2:117000/1‑126 (MQ=255)
                                                    cTGCTTTCTTCCTTTTTCATTCCGCAAGCCCCTATAAAAACAGCTAATAATACTAAATACAGCCAGAGCCTCAACCTTTTTGAGTGAATTATCATTTCACATCCCCATTTTTATTTTTGATGCAAAGTTAAGTTCAAAtt                                                                                      <  2:264087/140‑1 (MQ=255)
                                                    cTGCTTTCTTCCTTTTTCATTCCGCAAGCCCCTATAAAAACAGCTAATAATACTAAATACAGCCAGAGCCTCAACCTTTTTGAGTGAATCATCATTTCACATCCCCATTTTTATTTTTGATGCAAAGTTAAGTTCAAAtt                                                                                      <  1:650372/140‑1 (MQ=255)
                                                      gCTTTCTTCCTTTTTCATTCCGCAAGCCCCTATAAAAACAGCTAATAATACTAAATACAGCCAGAGCCTCAACCTTTTTGAGTGAATCATCATTTCACATCCCCATTTTTATTTTTGATGCAAAGTTAAGTTCAAATTcc                                                                                    <  1:46621/140‑1 (MQ=255)
                                                      gCTTTCTTCCTTTTTCATTCCGCAAGCCCCTATAAAAACAGCTAATAATACTAAATACAGCCAGAGCCTCAACCTTTTTGAGTGAATCATCATTTCACATCCCCATTTTTATTTTTGATGCAAAGTTAAGTTCAAATTcc                                                                                    <  2:682745/140‑1 (MQ=255)
                                                         ttcttcCTTTTTCATTCCGCAAGCCCCTATAAAAACAGCTAATAATACTAAATACAGCCAGAGCCTCAACCTTTTTGAGTGAATCATCATTTCACATCCCCATTTTTATTTTTGATGCAAAGTTAAGTTCAAATTCCAat                                                                                 >  1:313502/1‑140 (MQ=255)
                                                              ccTTTTTCATTCCGCAAGCCCCTATAAAAACAGCTAATAATACTAAATACAGCCAGAGCCTCAACCTTTTTGAGTGAATCATCATTTCACATCCCCATTTTTATTTTTGATGCAAAGTTGAGTTCAAATTCCAATATtaa                                                                            >  1:225666/1‑140 (MQ=255)
                                                               cTTTTTCATTCCGCAAGCCCCTATCAAAACCGCTAATAATACTAAATACAGCCAGAGCCTCCACCTTTTTGATTGCATCATCATTTCACATCCCCATTTTTATGTTTGATGCAAAGTTAAGTTCAAATTCCAATATtaat                                                                           <  2:459233/140‑1 (MQ=255)
                                                                cttttCATTCCGCAAGCCCCTATAAAAACAGCTAATAATACTAAATACAGCCAGAGCCTCAACCTTTTTGAGTGAATCATCATTTCACATCCCCATTTTTATTTTTGATGCAAAGTTAAGTTCAAATTCCAATATtaata                                                                          <  2:132767/139‑1 (MQ=255)
                                                                     cATTCCGCAAGCCCCTATAAAAACAGCTAATAATACTAAATACAGCCAGAGCCTCAACCTTTTTGAGTGAATCATCATTTCCCATCCCCATTTTTATTTTTGATGCAAAGTTAAGTTCAAATTCCAAAATTAATAATAtt                                                                     >  1:360751/1‑140 (MQ=255)
                                                                       ttCCGTAAGCCTCTATAATACCATTCAATAATACTAATTACAGCCAGAGCCTCAACCATTTTGAGTGAATCATCATTTCACATCCCCATTTTCATTTTTGATTCAAAGTTCAGTTCAAATTCCAAGATTAATAATATTag                                                                   <  2:568288/140‑1 (MQ=255)
                                                                               gCCCCTATAAAAACAGCTAATAATACTAAATACAGCCAGAGCCTCAACCTTTTTGAGTGAATCATCATTTCACATCCCCATTTTTATTTTTGATGCAAAGTTAAGTTCAAATTCCAATATTAATAATATTAGAGGAAAtt                                                           >  1:458908/1‑140 (MQ=255)
                                                                                          aacagccaccgatatTAAATACAGCCAGAGCCTCAACCTTTTTGAGTGAATCATCATTTAACATCCCCATTTTTATTTTTGATGCAAAGCTAAGATCAAATTCCAATATTAATAATATTAGAGGAAATTTGATGATTaaa                                                >  2:89124/12‑140 (MQ=255)
                                                                                          aaCAGCTAATAATACTAAATACAGCCAGAGCCTCAACCTTTTTGAGTGAATCCTCATTTCACATCCCCATTTTTATTTTTGATGCAAAGTTAAGTTCAAATTCAAATATTAATATTATTAGAGGAAATTTTATGATTaaa                                                >  1:291941/1‑140 (MQ=255)
                                                                                            taGCTAATAATACTAAATACAGCCAGAGCCTCAATCTTTTTGAGTGAATCATCATTTCACATCCCCATTTTTATTTTTGATGTAAAGTTAAGTTCAAATTCCAATATTAATAATATTAGAGGAAATTTTATGATTAAATc                                              <  2:169315/139‑1 (MQ=255)
                                                                                            cAGCTAATAATACTAAATACAGCCAGAGCCTCAACCTTTTTGAGTGAATCATCATTTCCCATCCCCATTTTTATTTTTGATGCAAAGTTAAGTTCAAATTCCAATATTAATAATATTAGAGGAAATTTTATGATTAAATc                                              <  2:569262/140‑1 (MQ=255)
                                                                                            cAGCTAATAATACTAAATACAGCCAGAGCCTCAACCTTTTTGAGTGAATCATCATTTCACATCCCCATTTTTATTTTTGATGCAAAGTTAAGTTCAAATTCCAATATTAATAATATTACAGGAAATTTTATGATTAAATc                                              >  2:281668/1‑140 (MQ=255)
                                                                                            cAGATAATAATACTAAATACAGCTAGTGCCTCAACCTTTTTGAGTGAATCATCATTTCACATCCCCATTTTTATTTTTAATGCAAAGTTAAGTTCAAATTCCAATATTAATAATATTAGAGAAGATTTTATGAttgtgtc                                              <  2:362292/140‑6 (MQ=255)
                                                                                             aGCTAATAATACTAAATACAGCCAGAGCCTCAACCTTTTTGTGTGAATCATCATTTCACATCCCCATTTTTATTTTTGATGCAAAGTTAAGTTCAAATTCCAATATTAATAATATTAGAGGAAATTTTATGATTAAATcc                                             <  2:91818/140‑1 (MQ=255)
                                                                                             aGCTAATAATACTAAATACAGCCAGAGCCTCAACCTTTTTGAGTGAATCATCATTTCACATCCCCATTTTTATTTTTGATGCAAAGTTAAGTTCAAATTCCAATATTAATAATATTAGAGGAAATTTTATGATTAAATcc                                             <  2:755703/140‑1 (MQ=255)
                                                                                             aGCTAATAATACTAAATACAGCCAGAGCCTCAACCTTTTTGAGTGAATCATCATTTCACATCCCCATTTTTATTTTTGATGCAAAGTTAAGTTCAAATTCCAATATTAATAATATTAGAGGAAATTTTATGATTAAATcc                                             <  1:267438/140‑1 (MQ=255)
                                                                                               cTAATAATACTAAATACAGCCAGAGCTTCAACCTTTTTGAGTGAATCATCATTTCACATCCCCATTTTTATTTTGGATGCAAAGTTAAGTTCAAATTCCAATATTAATAATATTAGAGGAAATTTTATGATTAAATCCTc                                           <  1:111418/140‑1 (MQ=255)
                                                                                               cTAATAATACTAAATACAGCCAGAGCCTCAACCTTTTTGAGTGAATCATCATTTCACATCCCCATTTTTTTTTTTGTTTTATTTTTATTTTCAAATTCCAATATTAATAATATTTGAGGAAATTCTATGATTaattactt                                           >  2:704611/1‑134 (MQ=255)
                                                                                               cTAATAATACTAAATACAGCCAGAGCCTCAACCTTTTTGAGTGAATCATCATTTCACATCCCCATTTTTATTTTTGATGCAAAGTTAAGTTCAAATTCCAATATTAATAATATTAGAGGAAATTTTATGATTAAATCCTc                                           >  1:387484/1‑140 (MQ=255)
                                                                                               cTAATAATACTAAATACAGCCAGAGCCTCAACCTTTTTGAGTGAATCATCATTTCACATCCCCATTTTTATTTTTGATGCAAAGTTAAGTTCAAATTCCAATATTAATAATATTAGAGACAATTTTATGTTTAACTCTTc                                           >  2:597704/1‑140 (MQ=255)
                                                                                                 aataatACTAAATACAGCCAGAGCCTCAACCTTTTTGAGTGGATCATCATTTCACATCCCCATTTTTATTTTTGATGCAAAGTTAAGTTCAAATTCCAATATTAATAATATTAGAGGAAATTTTATGATTAACACCTCGt                                         <  2:445533/140‑1 (MQ=255)
                                                                                                 aataatACTAAATACAGCCAGAGCCTCAACCTTTTTGAGTGGATCATCATTTCACATCCCCATTTTTATTTTTGATGCAAAGTTAAGTTCAAATTCCAATATTAATAATATTAGAGGAAATTTTATGATTAAATCCTCGt                                         <  2:556207/140‑1 (MQ=255)
                                                                                                 aataatACTAAATACAGCCAGAGCCTCAACCTTTTTGAGTGAATCATCATTTCACATCCCCATTTTTATTTTTGATGCAAAGTTAAGTTCAAATTCCAATATTAATAATATTAGAGGAAATTTTATGATTAAATCCTCGt                                         <  1:411011/140‑1 (MQ=255)
                                                                                                 aataatACTAAATACAGCCAGAGCCTCAACCTTTTTGAGTGAATCATCATTTCACATCCCCATTTTTATTTTTGATGCAAAGTTAAGTTCAAATTCCAATATTAATAATATTAGAGGAAATTTTATGATTAAATCCTCGt                                         >  2:205021/1‑140 (MQ=255)
                                                                                                 aataatACTAAATACAGCCAGAGCCTCAACCTTTTTGAGTGAATCATCATTTCACATCCCCATTTTTATTTTTGATGCAAAGTTAAGTTCAAATTCCAATATTAATAATATTAGAGGAAATTTTATGATTAAATCCTCGt                                         >  2:478242/1‑140 (MQ=255)
                                                                                                  ataataCTAAATACAGCCAGAGCCTCAACCTTTTTGAGTGAATCATCATTTCACATCCCCATTTTTATTTTTGATGCAAAGTTAAGTTCAAATTCCAATATTAATAATATTAGAGGAAATTTTATGATTAAATCCTCGTg                                        >  1:734086/1‑140 (MQ=255)
                                                                                                    aataCTAAATACAGCCAGAGCCTCAACCTTTTTGAGTGAATCATCATTTCACATCCCCATTTTTATTTTTGATGCAAAGTTAAGTTCAAATTCCAATATTAATAATATTAGAGGAAATTTTATGATTAAATCCTCGTGGa                                      >  1:107783/1‑140 (MQ=255)
                                                                                                    aataCTAAATACAGCCAGAGCCTCAACCTTTTTGAGTGAATCATCATTTCACATCCCCATTTTTATTTTTGATGCAAAGTTAAGTTCAAATTCCAATATTAATAATATTAGAGGAAATTTTATGATTAAATCCTCGTGGa                                      >  1:664577/1‑140 (MQ=255)
                                                                                                     ataCTAATTACTGCCAGTGCCTCAACCTTTTTGAGTGAATCTTCATTTCACATCCCCATTTTTATTTTTGATGCAAAGTTAAGTTCAAATTCCAATATTAATAATATTAGAGGAAATTTTATGATTAAATCCTCGTGGAt                                     <  1:58343/140‑1 (MQ=255)
                                                                                                     ataCTAAATACAGCCCGAGCCTCAACCTTTTTGAGTGAATCATCATTTCACATCCCCATTTTTATTTTTGATGCAAAGTTAAGTTCAAATTACAATATTAATAATATTAGAGGAAATTTTATGATTAAATCCTCGTGGAt                                     >  1:193313/1‑140 (MQ=255)
                                                                                                          aaaTACAGCCAGAGCCTCAACCTTTTTGAGTGAATCATCATTTCACATCCCCATTTTTATTTTTGATGCAAAGTTAAGTTCAAATTCCAATATTAATAATATTAGAGGAAATTTTATGATTAAATCCTCGTGGATTTTaa                                <  1:756564/140‑1 (MQ=255)
                                                                                                             tacacgcAGAGCCTTAATCTTTTTGAGCTGATCATCATTTCACCTCCCCATTTTTATTTTTGATGCAAAGTTAAGTTCAAATTCCAATATTAATAATATTAGAGGAAATTTTATGATTAAATCCTCGTGGATTTTAATTg                             <  1:169664/134‑1 (MQ=255)
                                                                                                               cAGCCAGAGCATTAACATTTTTCAGTGCATCATCATTTCACATCCCCTTTTTTATTTTTGTTGCTAAGTTAATTTGAAATTCCAATATTAATAATCTTAGAGGAAATTTTATGATTAAATCCTCGTGGATTTTAATTGtt                           <  1:673751/140‑1 (MQ=255)
                                                                                                                 gCCAGAGCCTCAACCTTTTTGAGTGAATCATCATTTCACATCCCCATTTTTATTTTTGATGCAAAGTTAAGTTCAAATTCCAATATTAATAATATTAGAGGAAATTTTATGATTAAATCCTCGTGGATTTTAATTGTTAg                         >  1:732935/1‑140 (MQ=255)
                                                                                                                 gCCAGAGCCTCAACCTTTTTGAGTGAATCATCATTTCACATCCCCATTTTTATTTTTGATGCAAAGTTAAGTTCAAATTCCAATATTAATAATATTAGAGGAAATTTTATGATTAAATCCTCGTGGATTTTAATTGTTAg                         >  1:648750/1‑140 (MQ=255)
                                                                                                                 gCCAGAGCCTCAACCTTTTTGAGTGAATCATCATTTCACATCCCCATTTTTATTTTTGATGCAAAGTTAAGTTCAAATTCAAATATTAATAATATTAGAGGAAATTTTATGATTAAATCCTCGTGGATTTTAATTGTTAg                         >  1:347873/1‑140 (MQ=255)
                                                                                                                   catagCCTCAACCTTTTTGAGTGAATCATCATTTCACATCCCCATTTTTATTTTTGATGCAAAGTTAAGTTCAAATTCCAATATTAATAATATTAGAGGAAATTTTATGATTAAATCCTCGTGGATTTTAATTGTTAGtt                       <  1:595354/137‑1 (MQ=255)
                                                                                                                     gagCCTCAACCTTTTTGAGTGAATCATCATTTCACATCCCCATTTTTATTTTTGATGCAAAGTTAAGTTCAAATTCCAATATTAATAATATTAGAGGAAATTTTATGATTAAATCCTCGTGGATTTTAATTGTTAGTTGt                     >  1:677811/1‑140 (MQ=255)
                                                                                                                     gagCCTCAACCTTTTTGAGTGAATCATCATTTCACATCCCCATTTTTATTTTTGATGCAAAGTTAAGTTCAAATTCCAATATTAATAATATTAGAGGAAATTTTATGATTAAATCATCGTGGATTTTAATTGTTAGTTGt                     >  1:717506/1‑140 (MQ=255)
                                                                                                                       gCCTCAACCTTTTTGAGTGAATCATCATTTCACATCCCCATTTTTATTTTTGATGCAAAGTTAAGTTCAAATTCCAATATTAATAATATTAGAGGAAATTTTATGATTAAATCCTCGTGGATTTTAATTGTTAGTTGTAt                   >  2:320920/1‑140 (MQ=255)
                                                                                                                        ccTCAACCTTTTTGAGTGAATCATCCTTTCACATCCCCATTTTTATTTTTGATGCAAAGTTAAGTTCAAATTCCAATATTAATAATATTAGAGGAAATTTTATGATTAAATCCTCGTGGGTTTTAATTGTTAGTTGTAtt                  >  2:421312/1‑140 (MQ=255)
                                                                                                                        ccTCAACCTTTTTGAGTGAATCATCATTTCACATCCCCATTTTTATTTTTGATGCAAAGTTAAGTTCAAATTCCAATATTAATAATATTAGAGGAAATTTTATGATTAAATCCTCGTGGATTTTAATTGTTAGTTGTAtt                  >  1:229596/1‑140 (MQ=255)
                                                                                                                                tttttGAGTGAATCATCATTTCACATCCCCAGTTTTATTTTTAATGCAAAGTTAAGTTCAAATTCCATTATTAATAATATTAGAGGAAATTTTATGATTAAATCCTAGTGGATTTTAATTGTTAGTTGTATTTTTCACat          >  2:180655/1‑140 (MQ=255)
                                                                                                                                   ttGAGTGAATCATCATTTCACATCCCCATTTTTATTTTTGATGCCAAGTTAAGTTCAAATTCCAATATTAATAATATTAGAGGTGTTCTTATGATTAAATCCTCGTGGATTTTAATTGTTAGTTGTATTTTTCACATATc       >  1:360092/1‑140 (MQ=255)
                                                                                                                                    ttttttaaTCATCATTTCACATCCCCATTTTTATTTTTGATGCAAAGTTAAGTTCAAATTCCATTATTAATAATATTAGAGCAAATTTTATGATTTAATCCTCGCGGATTTTAATTGTTAGTTGTATTTTTCACATATCa      >  2:334397/7‑140 (MQ=255)
                                                                                                                                    tGAGTGAATCATCATTTCACATCCCCATTTTTATTTTTGATGCAAAGTTAAGTTCAAATTCCAATATTAATAATATTAGAGGAAATTTTATGATTAAATCCTCGTGGATTTTAATTGTTAGTTGTATTTTTCACATATCa      <  1:565978/140‑1 (MQ=255)
                                                                                                                                       gTGAATCATCATTTCACATCCCCATTTTTATTTTTGATGCATAGTTAAGTTCAAATTCCAATATTAATAATATTAGAGGAAATTTTATGATTAAATCCTCGTGGATTTTAATTGTTAGTTGTATTTTTCACATATCAACa   >  1:520167/1‑140 (MQ=255)
                                                                                                                                        tGAATCATCATTTCACATCCCCATTTTTATTTTTGATGCAAAGTTAAGTTCAAATTCCAATATTAATAATATTAGAGGAAATTTTATGATTAAATCCTCGTGGATTTTAATTGTTAGTTGTATTTTTCACATATCAACAt  >  1:753618/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                         
TGGATACAGGCTCAACGTTTTGTTGAAGTTTTCTTTAATTTGTTTATCTTTACTGCTTTCTTCCTTTTTCATTCCGCAAGCCCCTATAAAAACAGCTAATAATACTAAATACAGCCAGAGCCTCAACCTTTTTGAGTGAATCATCATTTCACATCCCCATTTTTATTTTTGATGCAAAGTTAAGTTCAAATTCCAATATTAATAATATTAGAGGAAATTTTATGATTAAATCCTCGTGGATTTTAATTGTTAGTTGTATTTTTCACATATCAACAT  >  CP000730/2614524‑2614799

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑