BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	2,712,590	A→G	3.9%	S105P (TCA→CCA)	isaA ←	immunodominant antigen A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,712,590	0	A	G	3.9%	300.4 / 2.4	102	S105P (TCA→CCA)	isaA	immunodominant antigen A
Reads supporting (aligned to +/- strand): ref base A (50/48); new base G (2/2); total (52/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.98e-01

Only 100 of 105 total aligned reads displayed.

CATTGCTTAATCTCACTGAACTTGAAGTAGTTGAAGTGCTGTAGTTATGGTAAGTTGGAGCTGAAACAGCTTCAACGTTTGAGTTACTTGATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACCAGCAGTTTGACCATTAGCTGCTTCATAGCTCCATGACCATGTAGTACCATTTGAAGTGAAGTTATATTGGAAACCATCTTTTACAAAGTGGATGTCATATGCA  >  CP000730/2712452‑2712729

cATTGCTTAATCTCACTGAACTTGAAGTAGTTGAAGTGCTGTAGATATGGTAAGTTGGAGCTGAAACAGCTTCAACGTTTGAGTTACTTAATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTAGTATGaa                                                                                                                                            >  1:325935/1‑140 (MQ=255)

     tttAATCTCACTGAACTTGAAGTAGTTGAAGTGCTGTAGTTATGGTAAGTTGGAGCTGAAACAGCTTCAACGTTTGAGTTACTTGATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAagtagt                                                                                                                                       <  2:450952/139‑1 (MQ=255)

       tAATCTCACTGAACTTGAAGTAGTTTAATTGCTGTAGTTATGGGAAGTTGGAGCTGAAACAGCTTCAACGTTTGAGTTACTTGATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAgtagtgt                                                                                                                                     >  2:367267/1‑140 (MQ=255)

       tAATCTCACTGAACTTGAAGTAGTTGAAGTGCTGTAGTTATGGTAAGTTGGAGCTGAAACAGCTTCAACGTTTGAGTTACTTGATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAgtagtgt                                                                                                                                     >  1:166059/1‑140 (MQ=255)

       tAATCTCACTGAACTTGAAGTAGTTGAAGTGCTGTAGTTATGGTAAGTTGGAGCTGAAACAGCTTCAACGTTTGAGTTACTTGATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAgtagtgt                                                                                                                                     >  2:531806/1‑140 (MQ=255)

       tAATATCACTGAACTTGAAGTAGTTGAAGTGCTGTAGATATGGTAAGTTGGAGCTGAAACAGCTTCAACGTTTGAGTTACTTGATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAgtagagt                                                                                                                                     >  1:517602/1‑140 (MQ=255)

              aCTGAACTTGAAGTAGTTGAAGTGCTGTAGTTATGGTAAGTTGGAGCTGAAACAGCTTCAACGTTTGAGTTACTTGATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGc                                                                                                                              <  1:353736/140‑1 (MQ=255)

              aCTGAACTTGAAGTAGCTGAAGAGCTGTAGTTATGGTACGTTGGAGCTGAAACAGCGTCAGCGGTTGAGTTACTTGATTGTGCATTGTAGCTTACTGATTG‑TACAGTTGAGCCTTGGTTGTATGAAGTAGTGTAGTCTGc                                                                                                                              >  2:525219/1‑140 (MQ=255)

                tGAACTTGAAGTAGTTGAAGTGCTGTAGTTATGGTAAGTTGGAGCTGAAACAGCTTCAACGTTTTAGTTACTTGATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCAc                                                                                                                            <  1:222934/140‑1 (MQ=255)

                agAACTCGAAGGAGTNTAACTGCTGTAGTTATGGTAAGTTGGAGCTGAAACAGCTTCAACGTTTGAATTACTTGAATGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCAc                                                                                                                            <  1:600928/139‑1 (MQ=255)

                  aaCTTGAAGTAGTTGAAGTGCTGTAGTTATGGTAAGTTGGAGCTGAAACAGCTTCAACGTTTGAGTTACTTGATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCt                                                                                                                          <  1:96930/140‑1 (MQ=255)

                   aCTTGAAGTAGTTGAAGTGCTGTAGTTATGGTAAGTTGGAGCTGAAACAGCTTCAACGTTTGAGTTACTTGATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTg                                                                                                                         >  1:310214/1‑140 (MQ=255)

                    cTTGAAGTAGTTGAAGTGCTGTAGTTATGGTAAGTTGGAGCTGAAACAGCTTCAACGTTTGAGTTACTTGATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGc                                                                                                                        <  1:737504/140‑1 (MQ=255)

                    cTTGAAGTAGTTGAAGTGCTGTAGTTATGGTAAGTTGGAGCTGAAACAGCTTCAACGTTTGAGTTACTTGATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGc                                                                                                                        >  1:604860/1‑140 (MQ=255)

                    cTTGAAGTAGTTGAAGTGCTGTAGTTATGGTAAGTTGGAGCTGAAACAGCTTCAACGTTTGAGTTACTTGATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGc                                                                                                                        >  2:18884/1‑140 (MQ=255)

                    cTTGAAGTAGTTGAAGTGCTGTAGTTATGGTAAGTTGGAGCTGAAACAGCTTCAACGTTTGAGTTACTTGATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGAAGTGTAGTCTGCACCTGc                                                                                                                        >  1:340937/1‑140 (MQ=255)

                      tGAAGTATTTGAAGTGCTGTAGTTATGGTAAGTGGGAGCTGAAACAGCTTCAACGTTTGAGTTGCTTGATTGTGCATTGTAGCTTACTGGTTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCac                                                                                                                      >  2:294031/1‑139 (MQ=255)

                      tGAAGTAGTTGAAGTGATGTGGTAGGGGTAAGTTGGAGCTGAAACAGCTTCAACGTTTGAGTTACTTGATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTATTGTAGACTGCACCTGCaa                                                                                                                      >  1:181605/1‑140 (MQ=255)

                       gaagtaaacgacgggCTGGAGTTATGGTAAGTTAGACATGAAACAGCGTCAACGTTTGAGTTACTTGATTGTGCATTGTAGCTTACTGCTCGTTACTTTTG‑ACCTTGATTGTATGAAGTAGTCTATTCCGCACCTGCAAc                                                                                                                     >  2:518573/15‑140 (MQ=255)

                         aatagtCGAAGGGCTGTAATTATGGTAACCTGACACCTAAACAGCCTCAACCTCTGAGTTACTTAATTGTGCATTGTAGCTTACTGATTT‑TACATTCAAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGt                                                                                                                   <  2:344168/138‑1 (MQ=255)

                                gAAGTGCTGTAGTTATGGTAAGTTGGAGCTGAAACAGCTTCAACGTTTGAGTTACTTGATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGTTTGAGaa                                                                                                            <  1:723454/140‑1 (MQ=255)

                                   gTGCTGTAGTTATGGTCAGTTGGAGCTGAAACAGCTTCAACGTTTGAGTTACTTGATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGGAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAAcc                                                                                                         >  1:378462/1‑140 (MQ=255)

                                   gTGCTGTAGTTATGGTAAGTTGGAGCTGAAACAGCTTCAACGTTTGAGTTACTTGATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGGAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAAcc                                                                                                         >  1:539148/1‑140 (MQ=255)

                                    tGCTGGAGTTATGGTAGGTTGGAGCTGAAACAGCTTGAACGTTTGAGTTACTTGATTGTGCATTGGCGCTTACGGATTG‑GACATTTGAACCTTTGGTGTATGAAGTAGTGTAGTCTGCACCTGCGACGCTTGAGAAACca                                                                                                        <  2:36174/140‑1 (MQ=255)

                                     gCTGTAGTTATGGTAAGTTGGAGCTGAAACAGCTTCAACGTTTGAGTTACTTGATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACcag                                                                                                       <  1:612616/140‑1 (MQ=255)

                                      cTGTAGTTATGGTAAGTTGGAGCTGAAACAGCTTCAACGTTTGAGTTACTTGATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACcagc                                                                                                      <  2:15413/140‑1 (MQ=255)

                                      cTGTAGTTATGGTAAGTTGGAGCTGAAACAGCTTCAACGTTTGAGTTACTTGATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACcagc                                                                                                      >  2:233180/1‑140 (MQ=255)

                                      cTGTAGTTATGGTAAGTTGGAGCTGAAACAGCTTCAACGTTTGAGTTACTTGATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACcagc                                                                                                      <  2:357839/140‑1 (MQ=255)

                                      cTGTAGTTATGGTAAGTTGGAGCTGAAACAGCTTCAACGTTTGAGTTACTTGATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTAGGAAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACcagc                                                                                                      <  1:521887/140‑1 (MQ=255)

                                       tGTAGTTATGGTAAGTTGGAGCTGAAACAGCTTCAACGTTTGAGTTACTTGATTGTTCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTTTGCACCTGCAACGTTTGAGAAACcagca                                                                                                     >  1:63202/1‑140 (MQ=255)

                                        gTAGTTATGGTAAGTTGGAGCTGAAACAGCTTCAACGTTTGAGTTACTTGATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACcagcag                                                                                                    <  1:735723/140‑1 (MQ=255)

                                            ttATGGTAAGTTGGAGCTGAAACAGCTTCAACGTTTGAGTTACTTGATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACTTTTAAGAAACCAGCAGtttt                                                                                                >  1:758581/1‑139 (MQ=255)

                                               tGGTAGGTTGGCTCTGAAACCGCTTCACGGTTTGGTGTAATTGATTGTGCATTGTAGCTTACTGAGTG‑GACCTTTGAGCTGTGCTTGTATGAAGTCGTGTAGTCTGCGCCTGCAACGTTTGAGAAACCAGAATTTTGAcc                                                                                             <  2:325935/140‑1 (MQ=255)

                                               tGGTAAGTTGGAGTTGAAACAGCTTTTATTTTTGAGTTTTTTGATTGTGCATTGTAGCTTACTGATTG‑TACATTGGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACCAGCAGTTTGAcc                                                                                             <  1:302299/140‑1 (MQ=255)

                                                 gTAAGTTGGAGCTGAAACAGCTTCAACGTTTGAGTTACTTGATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACCAGCAGTTTGACCAt                                                                                           >  2:597887/1‑140 (MQ=255)

                                                 gTAAGTTGGAGCTGAAACAGCTTCAACGTTTGAGTTACTTGATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGACTGCAACTGCAACGTTTGAGAAACCAGCAGTTAGACCAt                                                                                           >  2:522192/1‑140 (MQ=255)

                                                  tAAGTTGGAGCTGAAACAGCTTCAACGTTTGAGTTACTTGATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACCAGCAGTTTGACCAtt                                                                                          >  1:173802/1‑140 (MQ=255)

                                                     gTTGGAGCTGAAACAGCTTCAACGTTTGAGTTACTTGATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTTTGGGCTGCACTTGCAACGTTTGAGAAACCAGCAGTTTGACCATTAgc                                                                                       >  1:57888/1‑140 (MQ=255)

                                                     gTTGGAGCTGAAACAGCTTCAACGTTTGAGTTACTTGATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACCAGCAGTTTGACCATTAgc                                                                                       >  2:165113/1‑140 (MQ=255)

                                                     gTTGGAGCTGAAACAGCTTCAACGTTTGAGTTACTTGATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACCAGCAGTTTGACCATTAgc                                                                                       <  1:375343/140‑1 (MQ=255)

                                                     gTTGGAGCTGAAACAGCTTCAACGTTTGAGTTACTTGATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACCAGCAGTTTGACCATTAgc                                                                                       >  1:533753/1‑140 (MQ=255)

                                                     gTTGGAGCTGAAACAGCTTCAACGTTTGAGTTACTTGATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACATTGGTTGTATGAAGTAGTGTAGTCTGCACATGCAACGTTTGAGAAACCAGCAGTTTGACCATTAgc                                                                                       >  1:478529/1‑140 (MQ=255)

                                                          accTGAAACAGCTTCAACGTTTGAGTTACTTGATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACCAGCAGTTTGACCATTAGCTGCtt                                                                                  <  2:619881/138‑1 (MQ=255)

                                                               aaaCAGCTTCAACGTTTGAGTTACTTGATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACCAGCAGTTTGACCATTAGCTGCTTCATAg                                                                             <  1:260435/140‑1 (MQ=255)

                                                               aaaCAGCTTCAACGTTTCGGTTACTTGATTGTGCATTTGAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGGAGTCTGCACCTGCAACGTTTGAGAAACCAGCAGTTTGACCATTAGCTGCTTCATAg                                                                             <  1:254639/140‑1 (MQ=255)

                                                                  cAGCTTCAACGTTTGAGTTACTTGATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACCAGCAGTTTGACCATTAGCTGCTTAATAGCTc                                                                          >  2:203272/1‑140 (MQ=255)

                                                                   aGCTTCAACGTTTGAGTTACTTGATTGTGCATTGTAGCTTACTGATTT‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCTCCTGTAACGTTTGAGAAACCAGCAGTTTGACCATTAGCTGCTTCATAGCTcc                                                                         <  2:106210/140‑1 (MQ=255)

                                                                   aGCTTCAACGTTTGAGTTACTTGATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACCAGCAGTTTGACCATTAGCTGCTTAATAGCTcc                                                                         >  1:248860/1‑140 (MQ=255)

                                                                   aGCTGCAACGTTTGAGTTTGTTGATTTGGCAGTGTAGCTTACTGGTTG‑TAGATTTTATCCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGTTTCAGAAACCAGCAGTTTGACCATTAGCTGCTTCATAGCTcc                                                                         <  2:502270/140‑1 (MQ=255)

                                                                    gCTTCAACGTTTGATTTACTTGATTGTGCATTGTAGCTTATTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACCAGCAGTTTGACCATTAGCTGCTTCATAGCTCCa                                                                        <  1:455357/140‑1 (MQ=255)

                                                                    gCTTCAACGTTTGAGTTACTTGATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACCAGCAGTTTGACCATTAGCTGCTTCATAGCTCCa                                                                        <  1:531806/140‑1 (MQ=255)

                                                                    gCTTCAACGTTTGAGTTACTTGATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACCAGAGGTTTCACCATTAGCTGCTTCATAGCTCCa                                                                        <  1:367267/140‑1 (MQ=255)

                                                                       tCAACGTTTGAGTTACTTGATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACCAGCAGTTTGACCATTAGCTGCTTCATAGCTCCATGa                                                                     >  2:566463/1‑140 (MQ=255)

                                                                        cAACGTTTGAGTTACTTGATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACCAGCAGTTTGACCATTAGCTGCTTCATAGCTCCATGAc                                                                    >  1:389604/1‑140 (MQ=255)

                                                                         aaCGTTTGAGTTACTTGATTGTGCATTTTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGTATCGTTTGAGAAACCAGCAGTTTGACCATTAGCTGCTTCATAGCTCCATGAcc                                                                   <  1:15691/140‑1 (MQ=255)

                                                                         aaCGTTTGAGTTACTTGATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACCAGCAGTTTGACCATTAGCTGCTTCATAGCTCCATGAcc                                                                   <  1:106181/140‑1 (MQ=255)

                                                                          aCGTTTGAGTTACTTGATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACCAGCAGTTTGACCATTAGCTGCTTCATAGCTCCATGACCa                                                                  >  1:109529/1‑140 (MQ=255)

                                                                               tGAGTTACTTGATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACCAGCAGTTTGACCATTAGCTGCTTCATAGCTCCATGACCATgtag                                                             >  1:526544/1‑140 (MQ=255)

                                                                                 aGTTACTTGATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACCAGCAGTTTGACCATTAGCTGCTTCATAGCTCCATGACCATgtagta                                                           >  1:290555/1‑140 (MQ=255)

                                                                                 aGTTACTTGATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACCAGCAGTTTGACCATTAGCTGCTTCATAGCTCCATGACCATgtagta                                                           >  1:678797/1‑140 (MQ=255)

                                                                                 aGTTACTTGATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACCAGCAGTTTGACCATTAGCTGCTTCATAGCTCCATGACCAGgtagta                                                           >  1:402120/1‑140 (MQ=255)

                                                                                   ttACTTGATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACCAGCAGTTTGACCATTAGCTGCTTCATAGCTCCATGACCATGTAGTAcc                                                         >  1:30203/1‑140 (MQ=255)

                                                                                    tACTTGATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACCAGCAGTTTGACCATTAGCTGCTTCATAGCTCCATGACCATGTAGTACCa                                                        >  1:28759/1‑140 (MQ=255)

                                                                                      cTTGATTGTGCATTTTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGTTTGAGACACCAGCAGTTTGACCATTAGCTGCTTCATAGCTCCATGACCATGTAGTACCAtt                                                      <  2:538179/140‑1 (MQ=255)

                                                                                      cTTGATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACCAGCTGTTTGACCATTAGCTGCTTCATAGCTCCATGACCATGTAGTACCAtt                                                      <  2:492922/140‑1 (MQ=255)

                                                                                      cTTGATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACCAGCAGTTTGACCATTAGCTGCTTCATAGCTCCATGACCATGTAGTACCAtt                                                      >  2:624196/1‑140 (MQ=255)

                                                                                      cTTGATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACCAGCAGTTTGACCATTAGCTGCTTCATAGCTCCATGACCATGTAGTACCAtt                                                      <  1:448311/140‑1 (MQ=255)

                                                                                      cTTGATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACCAGCAGTTTGACCATTAGCTGCTTCATAGCTCCATGACCATGTAGTACCAtt                                                      <  2:129821/140‑1 (MQ=255)

                                                                                       ttGATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACCAGCAGTTTGACCATTAGCTGCTTCATAGCTCCATGACCATGTAGTACCAttt                                                     <  2:653993/140‑1 (MQ=255)

                                                                                        tGATTGTGGCTGGTAGCTTACCGATCT‑TACATTTGACGCTTGGTTGCATGAATTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACCATCAGTTTGACCATTAGCTGCTTCATAGCTCCAGGACCATGTAGTACCATTtg                                                    <  2:448789/140‑1 (MQ=255)

                                                                                          agtgtgCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACCAGCAGTTTGACCATTAGCTGCTTCATAGCTCCATGACCATGTAGTACCATTtgaa                                                  <  2:160920/138‑1 (MQ=255)

                                                                                          aTTGTTCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACCAGCAGTTTGACCATTAGCTGCTTCATAGCTCCATGACCATGTAGTACCATTtgaa                                                  <  2:723372/140‑1 (MQ=255)

                                                                                             gtgCATTGTAGCGTACTGATTG‑TACATTAGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGGTTGAGAAACCAGCAGTTTGACCATTAGCTGCTTCATAGCTCCATGACCATGTAGTACCATTtgaagtg                                               <  2:268395/140‑1 (MQ=255)

                                                                                                cATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACCAGCAGTTTGACCATTAGCTGCTTCATAGCTCCATGACCATGTAGTACCATTtgaagtgaag                                            >  1:613029/1‑140 (MQ=255)

                                                                                                   tGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGTACCTGCAACGTTTGAGAAACCAGCAGTTTGACCATTAGCTGCTTCATAGCTCCATGACCATGTAGTACCATTTGAAGTGAAGTta                                         <  1:273091/140‑1 (MQ=255)

                                                                                                   tGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACCAGCAGTTTGACCATTAGCTGCTTCATAGCTCCATGACCATGTAGTACCATTTGAAGTGAAGTta                                         >  2:640837/1‑140 (MQ=255)

                                                                                                   tGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACCAGCAGATTGACCAATAGCTACTTCATAGCACCATAACCACGAAGTACCAGTGGAATTGAAGTta                                         >  1:284448/1‑140 (MQ=255)

                                                                                                    gTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTTTAGTCTGCACCTACAACGTTTGAGAAACCAGCAGTTTGACCATAACCTGCTTCATAGCTCCATGAACATGTAGTACCATTTGAAGTGAAGTtat                                        >  1:164278/1‑140 (MQ=255)

                                                                                                     tAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACCAGCAGTTTGACCATTAGCTGCTTCATAGCTCCATGACCATGTAGTACCATTTGAAGTGAAGTtata                                       <  2:173802/140‑1 (MQ=255)

                                                                                                       gCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGGAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACCAGCAGTTTGCCCATTAGCTGCTTCATAGCTCCATGACCATGTAGTACCATTTGAAGTGAAGTTATAtt                                     <  2:378462/140‑1 (MQ=255)

                                                                                                       gCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGGAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACCAGCAGTTTGACCATTAGCTGCTTCATAGCTCCATGACCAAGGAGTTCGATTTGAAGTGAAGTTATAtt                                     <  2:539148/140‑1 (MQ=255)

                                                                                                       gCTTACTGATTG‑TACATTTGAACATTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACCAGCAGTTTGACCATTAGCTGCTTCATAGCTGCCTGACCACGTAGTACCATTTGAAGTGACGTTatatg                                     >  2:161483/1‑139 (MQ=255)

                                                                                                           acgggttG‑GACATGTGCACCTTGGTTGTATGAAGTAGTGGAGTTTGCCCCTGCAACGTTTGCGAAACCAGCAGTTTGACCATTAGCTGCTTCATAGCTCCATGACCATGTAGTACCACTTGAAGGGAAGTTATATTGGaa                                 <  2:275637/135‑1 (MQ=255)

                                                                                                               aTTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACCAGCAGTTTGACCATTAGCTGCTTCATAGCTCCATGACCATGTAGTACCATTTGAAGTGAAGTTATATTGGAAACCa                             <  1:627356/140‑1 (MQ=255)

                                                                                                                attggtcATTTGAATCTTGGTTGTATGAAGTAGTGTAGTTTGTACCTGCAACGTTTGAGAAACCAGCAGTTTGACCATTAGCTGCTTCATAGCTCCATGACCATGTAGTACCATTTGAAGTGAAGTTATATTGGAAACCa                             <  1:35206/134‑1 (MQ=255)

                                                                                                                 tG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACCAGCAGTTTGACCATTAGCTGCTTCATAGCTCCATGACCATGTAGTACCATTTGAAGTGAAGTTATATTGGAAACCATc                           >  2:318864/1‑140 (MQ=255)

                                                                                                                 tG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACCAGCAGTTTGACCATTAGCTGCTTCATAGCTCCATGACCATGTAGTACCATTTGAAGTGAAGTTATATTGGAAACCATc                           >  1:590802/1‑140 (MQ=255)

                                                                                                                  g‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACCAGCAGTTTGACCATTAGCTGCTTCATAGCTCCATGACCATGTAGTACGATTTGAAGTGAAGTTATATTGGAAACCATCt                          >  2:660426/1‑140 (MQ=255)

                                                                                                                  g‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACCAGCAGTTTGACCATTAGCTGCTACATTGCCCCATGACCATGTAGAACCATTTGAAGTGAAGTTATATTGGAAACCATcc                          >  1:360216/1‑139 (MQ=255)

                                                                                                                    tACATTTGAACCTTGGTTGTATGAAGTAGTGTACTCTGCACCTGCAACGTCTGAGAAACCAGCAGTTTGACCATTAGCTGCTTCATAGCTCCATGACCATGTAGTACCATTTGAAGTGAAGTTATATTGGAAACCATCtt                         >  2:557211/1‑140 (MQ=255)

                                                                                                                     tcATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGTAACGTTTGAGAAACCAGCAGTTTGACCATTAGCTGCTTCATAGCTCCATGACCATGTAGTACCATTTGAAGTGAAGTTATATTGGAAACCATCttt                        <  1:672459/139‑1 (MQ=255)

                                                                                                                     aCATTTGAACCGTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACCAGCAGTTTGACCATTAGCTGCTTCATAGCTCCATGACCATGTAGTACCATTTGAAGTGAAGTTATATTGGAAACCATCttt                        <  2:137326/140‑1 (MQ=255)

                                                                                                                         ttGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACCAGCAGTTTGACCATTAGCTGCTTCATAGCTCCATGACCATGTAGTACCATTTGAAGGGAAGTTATATTGGAAACCATCTTTTACa                    >  2:378748/1‑140 (MQ=255)

                                                                                                                            acctttGGTTGTATGAAGTAGTGTAGTCTGCACCTGAAGTGTGTGATTTTCTAGTAGTTTGACCATTAGCTGCTTCATAGCTCCATGACCATGTAGTACCACTTGCAGTGAAGTTATATTGGAAACCATCTTTTACAAAg                 <  2:533753/136‑1 (MQ=255)

                                                                                                                            aaCCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACCAGCAGTTTGACCATTAGCTGCTTCATAGCTCCATGACCATGTAGTACCATTTGAAGTGAAGTTATATTGGAAACCATCTTTTACAAAg                 <  2:57888/140‑1 (MQ=255)

                                                                                                                            aaCCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACCAGCAGTTTGACCATTAGCTGCTTCATAGCTCCATGACCATGTAGTACCATTTGAAGTGAAGTTATATTGGAAACCATCTTTTACAAAg                 >  1:266918/1‑140 (MQ=255)

                                                                                                                             aCCTTGGTTGTATGAAGGAGGGTAGTCTGCACCTGCAACGTTTGAGAAACCAGCAGTTTGACCATTAGCTGCTTCATAGCTCCATGACCATGTAGTACCATTTGAAGTGAAGTTATATTGGAAACCATCTTTTACAAAGt                >  2:180792/1‑140 (MQ=255)

                                                                                                                             aCCTGGGTTGTATGAAGTAGTGTAGTTTGCACCTGCCAGGTTTGAGAAACCAGCAGTTTTACCATTAGCTGCTTCATAGCTTCATGACCATGTAGTACCATTTGAAGTGAAGTTATATTGGAAACCATCTTTTACAAAGt                <  1:586477/140‑1 (MQ=255)

                                                                                                                                   gTTGGATGAAGTAGTGTAGTCTGCACCTCCAACGTTTGAGAAACCAGCAGTTTGACCATTAGCTGCTTCATAGCTCCATGACCATGTAGTACCATTTGAAGTGAAGTTATATTGGAAACCATCTTTTACAAAGTgggtct          <  2:128789/140‑5 (MQ=255)

                                                                                                                                           aaGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACCAGCAGTTTGACCATTAGCTGCTTCATAGCTCCATGACCATGTAGTACCATTTGAAGTGAAGTTATATTGGAAACCATCTTTTACAAAGTGGATGTCATATGCa  <  2:674954/140‑1 (MQ=255)

CATTGCTTAATCTCACTGAACTTGAAGTAGTTGAAGTGCTGTAGTTATGGTAAGTTGGAGCTGAAACAGCTTCAACGTTTGAGTTACTTGATTGTGCATTGTAGCTTACTGATTG‑TACATTTGAACCTTGGTTGTATGAAGTAGTGTAGTCTGCACCTGCAACGTTTGAGAAACCAGCAGTTTGACCATTAGCTGCTTCATAGCTCCATGACCATGTAGTACCATTTGAAGTGAAGTTATATTGGAAACCATCTTTTACAAAGTGGATGTCATATGCA  >  CP000730/2712452‑2712729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`