Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 111,516 C→A 34.5% Q196K (CAA→AAA)  USA300HOU_0110 → possible staphylococcal tandem lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730111,5160CA34.5% 116.9 / 72.4 95Q196K (CAA→AAA) USA300HOU_0110possible staphylococcal tandem lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (35/27);  new base A (16/17);  total (51/44)
Fisher's exact test for biased strand distribution p-value = 5.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.04e-01

GAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAA  >  CP000730/111378‑111649
                                                                                                                                          |                                                                                                                                     
gAGGAAGTTAAAGAACAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCa                                                                                                                                      <  2:64200/140‑1 (MQ=255)
 aGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATACAGAGGTGCCGAGTTATTCAGCTCaa                                                                                                                                     >  1:34743/1‑140 (MQ=255)
  ggAAGTTAAAGAAGAAAACATAAAAAAAGCAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAat                                                                                                                                    <  2:484926/140‑1 (MQ=255)
  ggAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAat                                                                                                                                    <  1:314481/140‑1 (MQ=255)
  ggAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAat                                                                                                                                    <  1:15685/140‑1 (MQ=255)
  ggAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAat                                                                                                                                    <  2:622326/140‑1 (MQ=255)
   gAAGTTAAAGAAGAAAACATAAAAAAATTAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAata                                                                                                                                   <  1:625373/140‑1 (MQ=255)
   gAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGATGCCGAGTTATTCAGCTCAata                                                                                                                                   >  1:677887/1‑140 (MQ=255)
    aaattAAAGAAGAAAACATAAAAAAAGAAATCGAATACTTTTAGTTTTTTGCGCAATATAGTAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAatat                                                                                                                                  <  1:102967/137‑1 (MQ=17)
    aaGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATATTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATACAGAGGTGCCGAGTTATTAAGCTCAatat                                                                                                                                  >  2:613663/1‑140 (MQ=255)
     aGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGAAGATATATCATATAATTCAGAGATACCGAGTTATTCAGCTCAATATc                                                                                                                                 >  1:245854/1‑140 (MQ=255)
      gTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCACTATAGCAATTTTAAAGATTTAATGAATTATAAAGAAGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCACAATATCa                                                                                                                                <  2:561111/140‑1 (MQ=255)
      gTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCa                                                                                                                                <  1:521684/140‑1 (MQ=255)
      gTTAAAGAAGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGGGATAAATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCa                                                                                                                                <  1:146543/140‑1 (MQ=255)
       ttAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCaa                                                                                                                               >  2:26318/1‑140 (MQ=255)
        tAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGGAATTTTAAAGATTTAAGGAGTTAAAAAGATGGAGATATATCATATAATACAGAGGTGGCGAATTGTTCGGTGAAATATCAAt                                                                                                                              >  2:201882/1‑140 (MQ=17)
        tAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAAt                                                                                                                              <  2:399235/140‑1 (MQ=255)
         aaaGAAGAGAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCATTTTAGCAATTTTAAAGATTTTATGATTTTTTATGTTGGTGGTATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAAtt                                                                                                                             <  1:56476/140‑1 (MQ=17)
         aaaGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTACCGAGTTATTCGGCTAAATATCAAtt                                                                                                                             >  1:274761/1‑140 (MQ=255)
          aagaagAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTa                                                                                                                            >  1:467069/1‑140 (MQ=255)
            gaagaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAAc                                                                                                                          <  1:628576/140‑1 (MQ=255)
            gaagaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAAc                                                                                                                          <  1:73057/140‑1 (MQ=255)
            gaagaaAACATAAAAAAAGAAATCGAAAACTTTAAGTCTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAAc                                                                                                                          >  2:124687/1‑140 (MQ=255)
               gaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCTAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTaa                                                                                                                       >  2:720974/1‑140 (MQ=255)
               gaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAACATGGAGATATATCATATAATCCAGAGATGCCGAGTTATTCGGCTCAATATCAATTAACTaa                                                                                                                       >  1:485741/1‑140 (MQ=255)
                aaaaCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAat                                                                                                                      <  2:656231/140‑1 (MQ=255)
                aaaaCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATAAATTAACTAat                                                                                                                      >  1:762197/1‑140 (MQ=255)
                aaaaCATAAAAAAAGAAATCGAAAACTGTAAGGTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAat                                                                                                                      <  2:727646/140‑1 (MQ=18)
                  aaCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTTCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATACGGCGAAATATCAATTAAATAatga                                                                                                                    >  2:199593/1‑140 (MQ=17)
                    cATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAatgatg                                                                                                                  <  2:96582/140‑1 (MQ=255)
                      tAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATgatgat                                                                                                                >  1:432989/1‑140 (MQ=255)
                         aaaaaGAAATCGAAAACTTTACGTTTTTTGCGCAATATAGCTATTTTAAAGATTTAATGAATTATAAATATGGAGATATATAATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATtat                                                                                                             <  1:217898/140‑1 (MQ=255)
                         aaaaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATtat                                                                                                             <  1:433265/140‑1 (MQ=255)
                           aaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATaa                                                                                                           <  2:481891/140‑1 (MQ=255)
                            aataaaTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAATGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCATGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAAt                                                                                                          <  2:125613/137‑1 (MQ=255)
                            aaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATACAAATTATAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAAt                                                                                                          <  1:326810/140‑1 (MQ=255)
                              gAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTGATGATGATTATAATGt                                                                                                        >  1:510111/1‑140 (MQ=255)
                              gAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGt                                                                                                        >  2:153652/1‑140 (MQ=255)
                                aaTCGAATACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTCAATGAATCATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATCATGATTATAATGTaa                                                                                                      <  1:561361/140‑1 (MQ=17)
                                aaTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATAGCAATTAACTAATGATGATTATAATGTaa                                                                                                      <  1:149739/140‑1 (MQ=255)
                                aaTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTACAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaa                                                                                                      <  2:502279/140‑1 (MQ=255)
                                 aTCGAAAACTTTAATTTTTTTGCGAAATATAACAATTTTAAAGATTTAATGAATTATAAAGATAGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTaaa                                                                                                     <  2:376155/140‑1 (MQ=255)
                                 aTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTaaa                                                                                                     <  2:697153/140‑1 (MQ=255)
                                  tCGAAAACTTTAAGTTTTTTGCTCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaaaa                                                                                                    >  2:394502/1‑140 (MQ=255)
                                  tCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTaaaa                                                                                                    <  1:433696/140‑1 (MQ=255)
                                    gAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaaacaa                                                                                                  >  2:564772/1‑137 (MQ=255)
                                    gAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACa                                                                                                  >  2:263803/1‑140 (MQ=255)
                                      aaaCTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAAATATAAAGAAGGTTAAAGATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAAt                                                                                                <  1:748335/140‑1 (MQ=18)
                                       aaCTTTAAGTTTTTTGCGCAATATAGTAATTTTAAAGATTTGATGTATTATAAAGTTGGAGATATATCATATAATCTAGAGGTGCCGAGTTATTCAGCTCAATATCAATTATTTAATGATGATTATAATGTAAAACAttt                                                                                               >  1:207802/1‑140 (MQ=18)
                                       aaCTTTAAGTTCTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAAtt                                                                                               <  2:187935/140‑1 (MQ=255)
                                            tAAGTTTTTTGCGCAAGATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACgca                                                                                          >  2:542851/1‑138 (MQ=255)
                                              agtttttttttttttattGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTAAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACTTaaa                                                                                        >  2:84511/15‑140 (MQ=18)
                                               gTTTTTTTCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCACAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTaaaa                                                                                       <  1:201567/140‑1 (MQ=255)
                                                         gaaTATAGCAATCTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACAt                                                                             <  1:62493/139‑1 (MQ=255)
                                                               aGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATAccgac                                                                       <  2:431083/140‑4 (MQ=11)
                                                               aGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACcaac                                                                       <  1:524311/140‑1 (MQ=255)
                                                                 taaTTTTAAACATTTAATGAATTATAAAGATTGATATATATCATATAATCCAGAGGTGCCGAGTTATTCATCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACcaacaa                                                                     <  1:70535/139‑1 (MQ=255)
                                                                 cAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACcaacaa                                                                     <  1:75404/140‑1 (MQ=255)
                                                                  aaTTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATCTCAATTAACTAATGATGATTATAATGTAAAACACTTACGCAAAAGATATgctataacgatggt                                                                    >  2:716938/1‑127 (MQ=14)
                                                                  aaTTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACcgacgag                                                                    >  1:639605/1‑137 (MQ=11)
                                                                  aaTTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACaaa                                                                    <  1:702648/140‑1 (MQ=255)
                                                                     tttAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCGAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACcgacgagaaa                                                                 >  2:572793/1‑134 (MQ=11)
                                                                         aaGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAgcg                                                             >  1:413645/1‑140 (MQ=255)
                                                                          aGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCAACGAATAAAgctc                                                            >  1:288971/1‑138 (MQ=11)
                                                                             tttAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATAACATACCAACAAATAAAGCTCCGa                                                         >  2:505847/1‑140 (MQ=255)
                                                                             tttAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTAAGTAAAAGATATGACATACCAACAAATAAAGCGCCGa                                                         >  2:298759/1‑140 (MQ=255)
                                                                             tttAATGAATTATAAAGATGGAGATATATCATAGAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATAACATACCAACAAATAAAGCGCCGa                                                         >  1:753876/1‑140 (MQ=255)
                                                                                aaTGAATTATAAACATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGCTGATTATAATGTAAAACAATTACGCTAAAGATATGACATACCAACAAACAACGCGGCGACGc                                                      >  2:751029/1‑140 (MQ=17)
                                                                                 aTGAATTATAAAGATGGAGATATATCATATAATCCAGAGGCGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAGGCt                                                     >  1:650647/1‑140 (MQ=255)
                                                                                         taAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTAATGAGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGAAATACCAAAAAATAACGCAACGAAGCTGTTGTGGa                                             >  2:498684/1‑140 (MQ=14)
                                                                                           gaGATGGAAATATATCATATAATCCAGGGGTGGCAAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGaaa                                           <  2:690998/139‑1 (MQ=255)
                                                                                           acgATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATAAAGCGCAATATCAATTAACTAATGATGATTATAATATAAAACAATTACGTCACAGATATGACATGCAAACAAATAAAGCACCGAAACCGTTGATGcaa                                           >  2:318845/3‑140 (MQ=14)
                                                                                            aGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAg                                          >  2:591425/1‑140 (MQ=255)
                                                                                            aGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGAAAAAGATATGACATACAAACAAATAAAGCAACAAAGCTATTATTGAAAg                                          >  1:473743/1‑140 (MQ=14)
                                                                                            aGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATGACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAg                                          >  2:561274/1‑140 (MQ=255)
                                                                                                   gaTATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAgg                                   >  1:726760/1‑140 (MQ=255)
                                                                                                      atatCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGaa                                <  1:726488/140‑1 (MQ=255)
                                                                                                      atatCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGaa                                <  1:595883/140‑1 (MQ=255)
                                                                                                      atatCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGATGAAAGGTACAGGGaa                                >  1:128281/1‑140 (MQ=255)
                                                                                                      atatCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGAAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGaa                                >  1:193226/1‑140 (MQ=255)
                                                                                                               aaTCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAACGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAgg                       >  1:55692/1‑140 (MQ=255)
                                                                                                                aTCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGt                      >  1:155999/1‑140 (MQ=255)
                                                                                                                 tCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGtt                     >  1:711429/1‑140 (MQ=255)
                                                                                                                  tcAGAGGTGCCGTGTGAGTCGGCGAAATATCAATTAACTAATGATGATTATGAGGTAAAAGAATTACGTAAAAGTTATGAGAAACCCACAAAAGGAGCGCCGAAGCTGTTGTGGAAAGGTACAGGGCATTTAAAAGGTtc                    <  2:485741/139‑1 (MQ=2)
                                                                                                                   caaagGTGCCGAATTATTCAGCTCAATATCAATTAACTAATGAAGAATATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtca                   <  1:720974/137‑1 (MQ=255)
                                                                                                                   cAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtca                   >  2:362590/1‑140 (MQ=255)
                                                                                                                    agagGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtcat                  >  1:206617/1‑140 (MQ=255)
                                                                                                                     gagGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtcatc                 >  1:43364/1‑140 (MQ=255)
                                                                                                                     gagGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAACTTAAAAGGTtcatg                 >  2:716439/1‑139 (MQ=255)
                                                                                                                     gagGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTGGAAAGGTACAGGGAAATTAAAAGGTtcctc                 >  2:759849/1‑140 (MQ=255)
                                                                                                                        gTGCGGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGAACAGGGAATTTAGAAGGTTCATCAGt              >  1:542835/1‑140 (MQ=255)
                                                                                                                             gAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTGAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGat         >  2:660496/1‑140 (MQ=255)
                                                                                                                             gAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATAAGTTGGaa         >  2:37144/1‑139 (MQ=255)
                                                                                                                                  aatCAACTCAATAACAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGAAATGACATACCAACAAATAAAGCGCCGAAGCTGATGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaaa    <  1:227963/138‑1 (MQ=14)
                                                                                                                                    tCAGCTAAATATCAATTAACTAATGATGATTATAATTTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaaaaa  <  2:245854/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                     
GAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAA  >  CP000730/111378‑111649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: