Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	426,904	T→A	4.3%	E140V (GAA→GTA)	USA300HOU_0401 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	426,904	0	T	A	4.3%	256.7 / 2.5	84	E140V (GAA→GTA)	USA300HOU_0401	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (42/37);  new base A (2/2);  total (45/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

TTTTATTAACATCACCGGCATATTTTGTTGGCACGTCGACAACATTGATTTTATTTTGCGGTTGATAGCTAAGCTTTTCAATATCTTCATCAACATTGGCGATTGTACTATTTAAAGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCTTGAAATGCAAATCGTTTTTAACAGCTAAGTTAGATAAGTGGCTAAGTGTTTCTGCTTGTTTTTCAGTCACTTTTGTTTGACTGTCAATT  >  CP000730/426764‑427044                                                                                                                                             |                                                                                                                                             ttttATTAACATCACCGGCATATTTTGTTGGCACGTCGACAACATTGATTTTATTTTGCGGTTGATAGCTAAGCTTTTCAATATCTTCATCAACATTGGCGATTGTACTATTTAAAGCTTTGAAGTAATTCATCATTAAtt                                                                                                                                              <  2:787103/141‑1 (MQ=255) ttttATTAACATCACCGGCATATTTTGTTGGCACGTCGACAACATTGATTTTATTTTGCGGTTGATAGCTAAGCTTTTCAATATCTTCATCAACATTGGCGATTGTACTATTTAAAGCTTTGAAGTAATTCATCATTAAtt                                                                                                                                              >  2:558052/1‑141 (MQ=255)  tttATTAACATCACCAGCATATTTTGTTGGCACGTCGTCAACATTGATTTTATTTTGCGGTTGATAGCTAAGCTTTTCAATATCTTCATCAACACTGGCGATTGTACTATTTAAAGCTTTGAAGTAATCCATCATTGATTc                                                                                                                                             <  1:570330/141‑1 (MQ=255)     attaACATCACCGGCATATTTTGTTGGCACGTCGACAACATTGATTTTATTTTGCGGTTGATAGCTAAGCTTTTCAATATCTTCATCAACATTGGCGATTGTACTATTTAAAGCTTTGAAGTAATTCATCATTAATTCAAc                                                                                                                                          >  1:202646/1‑141 (MQ=255)           aTCACCGGCATATTTTGTTGGCACGTCTACAACATTGATTTTATTTTGCGGTTGATAGCTAAGCTTTTCAATATCTTCATCAACATTGGCGATTGTACTATTTAAAGCTTTGAAGTAATTCATCATTAATTCAACGGGttt                                                                                                                                    <  2:476028/141‑1 (MQ=255)               ccGGCATATTTTGTTGGCACGTCGACAACATTGATTTTATTTTGCGGTTGATAGCTAAGCTTTTCAATATCTTCATCAACATTGGCGATTGTACTATTTAAAGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCttt                                                                                                                                >  1:726837/1‑140 (MQ=255)               ccGGCATATTTTGTTGACACGGCGACAACATTGATTTTATTTTGCGGTTGATAGCTAAGCTTTTCAATATCTTCATCAACATTGGCGATTGTACTATTTAAAGCTTTGAAGTAATTTATCATTAATACAACGGGTTTCTta                                                                                                                                >  1:417094/1‑141 (MQ=255)                cGGCATATTTTGTTGGCACGTCGACAACATTGATTTTATTTTGCGGTTGATAGCTAAGCTTTTCAATATCTTCATCAACATTGGCGATTGTACTATTTAAAGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCTtat                                                                                                                               <  2:17786/141‑1 (MQ=255)                   cATATTTTGTTGGCACGTCGACAACATTGATTTTATTTTGCGGTTGATAGCTAAGCTTTTCAATATCTTCATCAACATTGGCGATTGTACTATTTAAAGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCTTATAtt                                                                                                                            >  2:583806/1‑141 (MQ=255)                   cATATTTTGTTGGCACGTCGACAACATTGATTTTATTTTGCGGTTGATAGCTAAGCTTTTCAATATCTTCATCAACATTGGCGATTGTACTATTTAAAGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCTTATAtt                                                                                                                            >  1:508503/1‑141 (MQ=255)                       ttttGTTGGCACGTCGACAACATTGATTTTATTTTGCGGTTGATAGCTAAGCTTTTCAACGACTTCATCAACATTGGCGATTGTACTATTTAAAGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCTTATATTCttt                                                                                                                        <  1:588561/141‑1 (MQ=255)                         ttgttgGCACGTCGACAACATTGATTTTATTTTGCGGTTGATAGCTAAGCTTTTCAATATCTTCATCAACATTGGCGATTGTACTATTTAAAGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAg                                                                                                                      >  1:705486/1‑141 (MQ=255)                           gttgGCACGTCGACAACATTGATTTTATTTTGCGGTTGATAGCTAAGCTTTTCAATATCTTCATCAACATTGGCGATTGTACTATTTAAAGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGa                                                                                                                    >  2:643671/1‑141 (MQ=255)                                    tCGACAACATTGATTTTATTTTGCGGTTGATAGCTAAGCTTTTCAATATCTTCATCAACATAGGCGATTGTACTATTTAAAGCTTTGAAGTAATGCATCATTAATTCCACGGGTTTCTTATATTCTTTAGGAATATTGttt                                                                                                           >  2:358617/1‑141 (MQ=255)                                          acaTTGATTTTATTTTGCGGTTGATAGCTAAGCTTTTCAATATCTTCATCAACATTGGCGATTGTACTATTTAAAGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTg                                                                                                     >  2:285067/1‑141 (MQ=255)                                          acaTTGATTTTATTTTGCGGTTGATAGCTAAGCTTTTCAATATCTTCATCAACATTGGCGATTGTACTATTTAAAGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTg                                                                                                     <  1:274293/141‑1 (MQ=255)                                          acaTTGATTTTATTTTGCGGTTGATAGCTAAGCTTTTCAATATCTTCATCAACATTGGCGATTGTACTATTTAAAGCTTTGAAGTAATTCATCAATAATTCAACGGGTTTCTTATCTTCTTTCGCAATATTGTTTTCAGTg                                                                                                     >  2:630927/1‑141 (MQ=255)                                           caTTGATTTTATTTTGCGGTTGATAGCTAAGCTTTTCAATATCTTCATCAACATTGGCGATTGTACTATTTAAAGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGa                                                                                                    <  1:453723/141‑1 (MQ=255)                                            aTTGATTTTATTTTGCGGTTGATAGCTAAGCTTTTCAATATCTTCATCAACATTGGCGATTGTACTATTTAAAGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGAc                                                                                                   <  1:7841/141‑1 (MQ=255)                                            aTTGATTTTATTTTGCCGTTGATAGCTAAGCTTTTCAATATCTACATCAACATTGGCGATTGTACTATTTAAAGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGAc                                                                                                   <  1:794643/141‑1 (MQ=255)                                              tGATTTTATTTTGCGGTTGATAGCTAAGCTTTTCAATATCTTCATCAACATTGGCGATTGTACTATTTAAAGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACaa                                                                                                 <  1:603751/141‑1 (MQ=255)                                               gATTTTATTTTGCGGTTGATAGCTAAGCTTTTCAATATCTTCATCAACATTGGCGATTGTACTATTTAAAGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACaaa                                                                                                >  1:520487/1‑141 (MQ=255)                                                tttttattttGCGGTTGATAGCTAAGCTTTTCAATATCTTCATCAACATTGGCGATTGTACTATTTAAAGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAAt                                                                                               <  2:252751/140‑1 (MQ=255)                                                 ttttattttGCGGTTGATAGCTAAGCTTTTCAATATCTTCATCAACATTGGCGATTGTACTATTTAAAGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAAtt                                                                                              >  2:43465/1‑141 (MQ=255)                                                 ttttattttGCGGTTGATAGCTAAGCTTTTCAATATCTTCATCAACATTGGCGATTGTACTATTTAAAGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAAtt                                                                                              >  2:642237/1‑141 (MQ=255)                                                 ttttattttGCGGTTGAGAGCTAAGCTTTTCAATATCTTCATCAACATTGTCGATTGTACTATTTAAAGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAAtt                                                                                              <  2:36859/141‑1 (MQ=255)                                                    tattttGCGGTTGATAGCTAAGCTTTTCAATATCTTCATCAACATTGGCGATTGTACTATTTAAAGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCt                                                                                           <  2:723742/141‑1 (MQ=255)                                                     attttGCGGTTGATAGCTAAGCTTTTCAATATCTTCATCAACATTGGCGATTGTACTATTTAAAGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCtt                                                                                          >  1:723939/1‑141 (MQ=255)                                                         tGCGGTTGATAGCTAAGCTTTTCAATATCTTCATCAACATTGGCGATTGTACTATTTAAATCTTGGAAGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCTTGaaa                                                                                      <  2:774384/141‑1 (MQ=255)                                                         tGCGGTTGATAGCTAAGCTTTTCAATATCTTCATCAACATTGGCGATTGTACTATTTAAAGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCTTGaaa                                                                                      <  1:249102/141‑1 (MQ=255)                                                         tGCGGTTGATAGCTAAGCTTTCCAATATCTTCATCAACATTGGCGATTGTACTATTTAAAGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTCTAGGAATATTGTTTTCAGTGACAAATTTCTTGaaa                                                                                      <  1:368567/141‑1 (MQ=255)                                                          gCGGTTGATAGCTAAGCTTTTCAATATCTTCATCAACATTGGCGATTGTACTATTTAAAGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTAAGTGACAAATTTCTTGAAAt                                                                                     >  2:767769/1‑141 (MQ=255)                                                           cGGTTGATAGCTAAGCTTTTCAATATCTTCATCAACATTGGCGATTGTACTATTTAAAGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCTTGAAATg                                                                                    >  2:738743/1‑141 (MQ=255)                                                           cGGTTGATAGCTAAGCTTTTCAATATCTTCATCAACATTGGCGATTGTACTATTTAAAGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCTTGAAATg                                                                                    >  2:757765/1‑141 (MQ=255)                                                            ggTTGATAGTTAAGTTTTTCAATATCTTCATCAACATTGGCGATTGTAATATTTAAAGCTTTGAAGTAATTCATCATTAATACAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCTTGAAATGc                                                                                   <  1:634222/141‑1 (MQ=255)                                                            ggTTGATAGTTAAGCTTTTCCATGTCTTACTGAACATTGGCGATTGTACTATTTAAAGCTTTGAAGTAATTCATCATTAATACATCGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGAGACAAATTTCTTGAAATGc                                                                                   <  1:430124/141‑1 (MQ=255)                                                            ggTTGATAGCTTAGCTTTTCAATATCTTCATCAACATTGGCGATTGTACTATTTAAAGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCTTGAAATGc                                                                                   <  1:436835/141‑1 (MQ=255)                                                            ggTTGATAGCTAAGCTTTTCAATAACTTCATCAACATGGGCGATTGTACTATTTAAAGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCTTGAAATga                                                                                   >  1:580804/1‑140 (MQ=255)                                                                 aTAGCTAAGCTTTTCAATATCTTCATCAACATTGGCGATTGTACTATTTAAAGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCTTGAAATGCAAATc                                                                              <  2:472521/141‑1 (MQ=255)                                                                  tAGCTAAGTTTTTCAATATCTTCATCAACATTGGCGATTGTACTATTTAAAGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCTTGAAATGCAAATCg                                                                             <  2:297142/141‑1 (MQ=255)                                                                  tAGCTAAGCTTTTCAATATCTTCATCAACATTGGCGATTGTACTATTTAAAGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCTTGAAATGCAAATCg                                                                             <  2:630082/141‑1 (MQ=255)                                                                  tAGCTAACCTTTTCAATATCTGCATCAACATTGGCGATTGTACTATTTAAAGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCTTGAAATGCAAATCg                                                                             <  2:297658/141‑1 (MQ=255)                                                                  aaGCTAAGCTTTTCAATATCTTCATCAACATTGGCGATTGTACTATTTAAAGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCTTGAAATGCAAATCg                                                                             <  2:31221/140‑1 (MQ=255)                                                                    gCTAAGCTTTTCAATATCTTCATCAACATTGGCGATTGTACTATTTAAAGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCTTGAAATGCAAATCGtt                                                                           >  2:422060/1‑141 (MQ=255)                                                                    gCTAAGCTTTTCAATATCTTCATCAACATTGGCGATTGTACTATTTAAAGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCTTGAAATGCAAATCGtt                                                                           >  1:737458/1‑141 (MQ=255)                                                                    gCTAAGCTTTTCAATATCTTCATCAACATTGGCGATTGTACTATTTAAAGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCTTGAAATGCAAATCGtt                                                                           >  2:777522/1‑141 (MQ=255)                                                                             ttCAATATCTTCATCAACATTGGCGATTGTACTATTTAAAGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCTTGAAATGCAAATCGTTTTTAACAGc                                                                  >  2:723099/1‑141 (MQ=255)                                                                              tCAATATCTTCATCAACATTGGCGATTGTACTATTTAAAGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCTTGAAATGCAAATCGTTTTTAACAGCt                                                                 >  2:368012/1‑141 (MQ=255)                                                                               cAATATCTTCATCAACATTGGCGATTGTACTATTTAAAGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCTTGAAATGCAAATCGTTTTTAACAGCTa                                                                >  1:228186/1‑141 (MQ=255)                                                                               cAATATCTTCATCAACATTGGCGATTGTACTATTTAAAGCTTTGAAGTAATTCATCATTAATACAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCTTGAAATGCAAATCGTTTTTAACAGCTa                                                                >  2:773291/1‑141 (MQ=255)                                                                                          tcaACATTGGCGATTGTACTATTTAAAGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCTTGAAATGCAAATCGTTTTTAACAGCTAAGTTAGATAAg                                                     <  1:808366/141‑1 (MQ=255)                                                                                           caACATTGGCGATTGTACTATTTAAAGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCTTGAAATGCAAGTCGTTTTTAACAGCTAAGTTAGATAAGt                                                    >  1:562855/1‑141 (MQ=255)                                                                                            aaCATTGGCGATTGTACTATTTAAAGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCTTGAAATGCAAATCGTTTTTAACAGCTAAGTTAGATAAGTg                                                   >  2:209878/1‑141 (MQ=255)                                                                                             aCATTGGCGATTGTACTATTTAAAGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCTTGAAATGCAAATCGTTTTTAACAGCTAAGTTAGTTAAGTgg                                                  >  2:293961/1‑141 (MQ=255)                                                                                             aCATTGGCGATTGTACTATTTAAAGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCTTGAAATGCAAATAGTTTTTAACAGCTAAGTTAGATAAGTgg                                                  >  1:262355/1‑141 (MQ=255)                                                                                                  ggCGATTGTACTATTTAAAGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCTTGAAATGCAAATCGTTTTTAACAGCTAAGTTAGATAAGTGGCTAAg                                             <  2:202646/141‑1 (MQ=255)                                                                                                  ggCGATTGTACTATTTAAAGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCTTGAAATGCAAATCGTTTTTAACAGCTAAGTTAGATAAGTGGCTAAg                                             >  2:608394/1‑141 (MQ=255)                                                                                                    ggATTGTACTATTTAAAGCTTTGAAGTAATTCATCATTAATTCAACTGGTATCTTTTATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCTTGAAATGCAAATCGTTTTTAACAGCTAAGTTAGATAAGTGGCTAAgtg                                           <  2:420546/140‑1 (MQ=255)                                                                                                    cGATTGTACTATTTAAAGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCTTGAAATGCAAATCGTTTTTAACAGCTAAGTTAGATAAGTGGCTAAgtg                                           >  2:698325/1‑141 (MQ=255)                                                                                                    agatggcaCTATTTAAAGCTTTGAAGTAATTCAGCATTAATTCAGCGGGAAGCTTATATTGTTTAGGAATATGGTTTTTAGTGACAGAATTCTTTGAATGCAAATCGTTTTTAACAGCTAAGTTTTGTATGGGGCTAAgtg                                           >  2:286258/8‑141 (MQ=255)                                                                                                        tGTACTATTTAAAGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCTTGAAATGCAAATCGTTTTTAACAGCTAAGTTAGATAAGTGGCTAAGTGTTTc                                       <  1:303984/141‑1 (MQ=255)                                                                                                         gTACTATTTAAAGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCTTGAAATGCAAATCGTTTTTAACAGCTAAGTTAGATAAGTGGCTAAGTGTTTCt                                      <  1:256666/141‑1 (MQ=255)                                                                                                                 tAAAGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCTTGAAATGCAAATCGTTTTTAACAGCTAAGTTAGATAAGTGGCTAAGTGTTTCTGCTTGttt                              <  1:295929/141‑1 (MQ=255)                                                                                                                  aaaGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCTTGAAATGCAAATCGTTTTTAACAGCTAAGTTAGATAAGTGGCTAAGTGTTTCTGCTTGtttt                             <  1:539085/141‑1 (MQ=255)                                                                                                                  aaaGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCTTGAAATGCAAATCGTTTTTAACAGCTAAGTTAGATAAGTGGCTAAGTGTTTCTGCTTGtttt                             >  1:410254/1‑141 (MQ=255)                                                                                                                  aaaGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCGTGAAGGGCAAGTCGTTTTTAACAGCTAAGTTAGATAAGTGGCTAAGTGTTTCTGCTTGtttt                             >  1:359544/1‑141 (MQ=255)                                                                                                                  aaaGCTTTGAAGTAATGCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCTTGAAATGCAAATCGTTTTTAACAGCTAAGTTAGATAAGTGGCTAAGTGTTTCTGCTTGtttt                             >  1:481272/1‑141 (MQ=255)                                                                                                                   aaGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCTTGAAATGCAAATCGTTTTTAACAGCTAAGTTAGATAAGTGGCTAAGTGTTTCTGCTTGttttt                            >  1:306064/1‑141 (MQ=255)                                                                                                                   aaGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCTTGAAATGCAAATCGTTTTTAACAGCTAAGTTAGATAAGTGGCTAAGTGTTTCTGCTTGttttt                            >  1:520764/1‑141 (MQ=255)                                                                                                                        ttGAAGTAATTCATCATTAATGCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCTTGAAATGCAAATCGTTTTTAACAGCTAAGTTAGATAAGTGGCTAAGTGTTTCTGCTTGTTTTTCAGTc                       >  2:780356/1‑141 (MQ=255)                                                                                                                         tGAAGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCTTGAAATGCAAATCGTTTTTAACAGCTAAGTTAGATAAGTGGCTAAGTGTTTCTGCTTGTTTTTCAGTCa                      <  2:705486/141‑1 (MQ=255)                                                                                                                           aaGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCTTGAAATGCACATCGTTTTTAACAGCTAAGTTAGATAAGTGGCTAAGTGTTTCTGCTTGTTTTTCAGTCACt                    >  1:531793/1‑141 (MQ=255)                                                                                                                           aaGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCTTGAAATGCAAATCGTTTTTAACAGCTAAGTTAGATAAGTGGCTAAGTGTTTCTGCTTGTTTTTCAGTCACt                    >  1:777719/1‑141 (MQ=255)                                                                                                                            aGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCTTGAAATGCAAATCGTTTTTAACAGCTCAGTTAGATAAGTGGCTAAGTGTTTCTGCTTGTTTTTCAGTCACtt                   <  2:737458/141‑1 (MQ=255)                                                                                                                            aGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCTTGAAATGCAAATCGTTTTTAACAGCTAAGTTAGATAAGTGGCTAAGTGTTTCTGCTTGTTTTTCAGTCACtt                   <  1:777522/141‑1 (MQ=255)                                                                                                                            aGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCTTGAAATGCAAATCGTTTTTAACAGCTAAGTTAGATAAGTGGCTAAGTGTTTCTGCTTGTTTTTCAGTCACtt                   <  1:422060/141‑1 (MQ=255)                                                                                                                             gTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCTTGAAATGCAAATCGTTTTTAACAGCTAAGTTAGATAAGTGGCTAAGTGTTTCTGCTTGTTTTTCAGTCACttt                  >  1:226074/1‑141 (MQ=255)                                                                                                                               aaTTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCTTGAAATGCAAATCGTTTTTAACAGCTAAGTTAGATAAGTGGCTAAGTGTTTCTGCTTGTTTTTCAGTCACTtttg                <  1:423888/141‑1 (MQ=255)                                                                                                                                aTTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCTTGAAATGCAAATCGTTTTTAACAGCTAAGTTAGATAAGTGGCTAAGTGTTTCTGCTTGTTTTTCAGTCACTtttgt               <  1:738743/141‑1 (MQ=255)                                                                                                                                aTTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCTTGAAATGCAAATCGTTTTTAACAGCTAAGTTAGATAAGTGGCTAAGTGTTTCTGCTTGTTTTTCAGTCACTtttgt               >  1:772862/1‑141 (MQ=255)                                                                                                                                aTTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCTTGAAATGCAAATCGTTTTTAACAGCTAAGTTAGATAAGTGGCTAAGTGTTTCTGCTTGTTTTTCAGTCACTtttgt               >  1:610192/1‑141 (MQ=255)                                                                                                                                aTTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCTTGAAATGCAAATCGTTTTTAACAGCTAAGTTAGATAAGTGGCTAAGTGTTTCTGCTTGTTTTTCAGTCACTtttgt               <  1:320487/141‑1 (MQ=255)                                                                                                                                aTTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCTTGAAATGCAAATCGTTTTTAACAGCTAAGTTAGATAAGTGGCTAAGTGTTTCTGCTTGTTTTTCAGTCACTtttgt               <  1:757765/141‑1 (MQ=255)                                                                                                                                aTTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCTTGAAATGCAAATCGTTTTTAACAGCTAAGTTAGATAAGTGGCTAAGTGTTTCTGCTTGTTTTTAAGTGACTtttgt               >  1:232187/1‑141 (MQ=255)                                                                                                                                aTTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTAAGTGACAAATTTCTTGAAATGCAAATCGTTTTTAACAGCTAAGTTAGGTAAGTGGCTACGTGTTTCTGCGTGTTTTTCAGTCACTtttgt               >  1:536232/1‑141 (MQ=255)                                                                                                                                       atTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCTTGAAATGCAAATCGTTTTTAACAGCTAAGTTAGATCAGCGGCTAAGTGTTTCTGCTTGTTTTTCAGTCACTTTTGTTTGACTg        <  1:630927/141‑1 (MQ=255)                                                                                                                                             tCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCTTGAAATGCAAATCGTTTTTAACAGCTAAGTTAGATAAGTGGCTAAGTGTTTCTGCTTGTTTTTCAGTCCCTGTTGTTTGACTGTCAAtt  >  1:358169/1‑141 (MQ=255)                                                                                                                                             |                                                                                                                                             TTTTATTAACATCACCGGCATATTTTGTTGGCACGTCGACAACATTGATTTTATTTTGCGGTTGATAGCTAAGCTTTTCAATATCTTCATCAACATTGGCGATTGTACTATTTAAAGCTTTGAAGTAATTCATCATTAATTCAACGGGTTTCTTATATTCTTTAGGAATATTGTTTTCAGTGACAAATTTCTTGAAATGCAAATCGTTTTTAACAGCTAAGTTAGATAAGTGGCTAAGTGTTTCTGCTTGTTTTTCAGTCACTTTTGTTTGACTGTCAATT  >  CP000730/426764‑427044

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑