Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,416,458 A→T 100% I216N (ATT→AAT)  USA300HOU_1314 ← oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,416,4580AT100.0% 185.5 / NA 55I216N (ATT→AAT) USA300HOU_1314oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (25/30);  total (25/30)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AACTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAAATGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTGGTTCG  >  CP000730/1416325‑1416595
                                                                                                                                     |                                                                                                                                         
aaCTCAATCCTAGTAATCCCTCTTCAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTg                                                                                                                                    <  1:32302/141‑1 (MQ=255)
 aCTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGa                                                                                                                                   <  1:486304/141‑1 (MQ=255)
  cTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGAc                                                                                                                                  <  2:317463/141‑1 (MQ=255)
  cTCAATAATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTCTACGGATGCAAATGCTCATACTTGAAATGATTCATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGaa                                                                                                                                  >  2:509916/1‑140 (MQ=255)
        cATCGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATAtt                                                                                                                            <  2:768970/141‑1 (MQ=255)
        cATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATAtt                                                                                                                            <  1:423616/141‑1 (MQ=255)
        cATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATAtt                                                                                                                            <  2:134161/141‑1 (MQ=255)
          tAGTAATCCCTCTTTAATTGTGTTCTATATTTACTTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTc                                                                                                                          <  2:453194/141‑1 (MQ=255)
           aGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTAATAATTGAAAGGAGTAACACAACCATTTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCa                                                                                                                         >  1:352323/1‑141 (MQ=255)
                    cccttAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCTATACATGAc                                                                                                                <  1:593400/138‑1 (MQ=255)
                           ttttgtttcatatGTAATGAGCCGTTCAGTATACTGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGtt                                                                                                         <  1:530310/132‑1 (MQ=255)
                              tgtTCTATATTTAATTAGACGTTCAGTATACGGATTCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTAtg                                                                                                      <  2:367825/141‑1 (MQ=255)
                                         tAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGAtaataa                                                                                           >  1:714685/1‑141 (MQ=255)
                                         tAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACAGTACTTATGTTATGTGTGAtaataa                                                                                           >  2:505902/1‑141 (MQ=255)
                                                aCGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATAGATGACTTAAGTTATGTGTGATAATAAATAATGt                                                                                    <  1:797602/141‑1 (MQ=255)
                                                     cAGTATACGGATGCAAATGCTCCTATTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGAc                                                                               >  1:393590/1‑141 (MQ=255)
                                                     cAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGAc                                                                               >  1:266270/1‑141 (MQ=255)
                                                      aGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGAcc                                                                              <  2:737627/141‑1 (MQ=255)
                                                       gTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCa                                                                             <  2:84576/141‑1 (MQ=255)
                                                       gTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCa                                                                             <  1:466417/141‑1 (MQ=255)
                                                       gTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCa                                                                             <  2:17594/141‑1 (MQ=255)
                                                       gTATACGGATGAAAATGCTCATACTTGAAATGATTAATTTTCCCTCGTTGAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCa                                                                             <  1:548443/141‑1 (MQ=255)
                                                        tataCGGATGCAAATGCTCAAACTTGAAATTATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCAt                                                                            <  1:73448/141‑1 (MQ=255)
                                                          taCGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACTTGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGt                                                                          <  2:133503/141‑1 (MQ=255)
                                                          taCGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGt                                                                          <  1:284512/141‑1 (MQ=255)
                                                          taCGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGt                                                                          <  2:261983/141‑1 (MQ=255)
                                                          taCGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATGTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGt                                                                          <  2:733731/141‑1 (MQ=255)
                                                            cGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTc                                                                        >  1:209140/1‑141 (MQ=255)
                                                            cGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTc                                                                        >  2:577310/1‑141 (MQ=255)
                                                                tGCAAATGCTCATACTCGATATGATTAATATTACCTCGATCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAAt                                                                    >  1:452942/1‑141 (MQ=255)
                                                                   aaaTGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACa                                                                 >  2:350387/1‑141 (MQ=255)
                                                                   aaaTGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACa                                                                 <  1:324212/141‑1 (MQ=255)
                                                                     aTGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAat                                                               >  1:807959/1‑141 (MQ=255)
                                                                        cTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATg                                                            >  1:169643/1‑141 (MQ=255)
                                                                        cTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTATGAAAACATAATTGTACTGACAATATTTCACTACATGACTTAAGTTAAGTGTGATAATAAATAATATTTGACCATGTTCTAATACAttatc                                                            >  2:280293/1‑140 (MQ=255)
                                                                          cATACTTGAAATGATTAATATTAGCTCGTTCAATGATTTGACCTTCTTTTAAAACATAGTTGTACTGACAATATTTCAAGACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACGATGTTCTAATACCATATGCt                                                          <  1:509916/141‑1 (MQ=255)
                                                                          cATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCt                                                          >  1:633375/1‑141 (MQ=255)
                                                                                       aTTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCAt                                             >  2:687669/1‑141 (MQ=255)
                                                                                       aTTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAAAGAATcttt                                             >  1:748577/1‑138 (MQ=255)
                                                                                       aTTAATATTACCTCGTTAAATGATTTGACTGACGCGAAAAACAAAATTGTTCTGACAAAATTTCAATACATGACTTAAGTTATATGTGATAAGAAATAATAGATTAACATGTTCTAATACACTATGTTGtaataactgcag                                             >  2:136430/1‑135 (MQ=255)
                                                                                         tAATATTACCTCGTTCAATGATTTGACCTTCTTTTAACACCTCCTGGTACTTACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCa                                           <  1:548005/141‑1 (MQ=255)
                                                                                              ttACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGa                                      >  1:765199/1‑141 (MQ=255)
                                                                                                aCCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATACTAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGAtt                                    >  2:648402/1‑141 (MQ=255)
                                                                                                       tCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGCCTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATAACTTGATTACCGTTc                             >  1:121874/1‑141 (MQ=255)
                                                                                                          aTGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCaaa                          <  1:577310/141‑1 (MQ=255)
                                                                                                          aTGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCaaa                          >  2:804699/1‑141 (MQ=255)
                                                                                                          aTGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTAATTACCGTTCcaa                          >  2:520145/1‑141 (MQ=255)
                                                                                                          aTGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATACATCACTTGATTACCGTTCaaa                          <  2:209140/141‑1 (MQ=255)
                                                                                                              tttGACCTTCTTTTAAAACATAATTGTACTGACAATATTGCAATACACGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCAGGTTCTAATACAATATGCTGTAATAAAACCATCACTTGATTAACGTTCaaggaat                      >  2:506258/1‑136 (MQ=255)
                                                                                                               ttGACCCTCCTTTAAAACATAATTGCACTGACTATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCTCTTGATTACCGTTCAAAGCATc                     <  1:92025/141‑1 (MQ=255)
                                                                                                                      tcttTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAatgat              <  2:393590/141‑1 (MQ=255)
                                                                                                                          ttAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCaa          >  1:131517/1‑141 (MQ=255)
                                                                                                                           tAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATACAAc         >  1:31594/1‑141 (MQ=255)
                                                                                                                              aaCATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTgg      <  1:650539/141‑1 (MQ=255)
                                                                                                                                  tAATTGTACTGACAAAATTTCCATACAACACAACACTTACGTGTGCTAATAAATATCGATTTGCTGTGTTCTGGGGCGATATGCTTTGGTTAATCCATCGCTTGGGTACCGTTCAAAGCATCCAATGATGCAACTGGTTCg  <  2:470611/141‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                         
AACTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTGAAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAAATGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTGGTTCG  >  CP000730/1416325‑1416595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑