Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,744,362 G→A 100% H104Y (CAC→TAC)  apt ← adenine phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,744,3620GA100.0% 172.4 / ‑6.5 60H104Y (CAC→TAC) aptadenine phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (23/36);  minor base C (0/1);  total (23/37)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTCAATTATAAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAA  >  CP000730/1744225‑1744494
                                                                                                                                         |                                                                                                                                    
ttcaattATAAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACAt                                                                                                                                   <  1:807554/141‑1 (MQ=255)
   aattATAAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTCGTTTAATTGCATCTTTGTACATTGt                                                                                                                                >  2:311437/1‑141 (MQ=255)
                aTACCTACTACGATACCGCCTAATTTTTCACCTTAGTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTa                                                                                                                   >  1:192709/1‑141 (MQ=255)
                  aCCTACTACGATACCGCCTAATTTTTCAACTTACCCAGCAGCTACTTAAATTCTCCCACCATTAGCTAATAAATCATCTGTAATTAACACACCTTGACCTGGTTTAATTGCATCTTGGTACATTCTTAAAACAGTTGTGcc                                                                                                                 >  1:796805/1‑141 (MQ=255)
                  aCCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTAcc                                                                                                                 <  2:396511/141‑1 (MQ=255)
                  aCCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTAcc                                                                                                                 >  1:794968/1‑141 (MQ=255)
                    ctactaCGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCat                                                                                                               >  2:561395/1‑141 (MQ=255)
                       ctacGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCATCAATCATACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATAtt                                                                                                            >  2:525291/1‑141 (MQ=255)
                          tgctACCGCATAATTTCTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCGTTGTACATTGTTAAAACATTTGTACCATATTCTa                                                                                                         <  2:353341/138‑1 (MQ=255)
                          cGATACCTCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTa                                                                                                         <  1:679396/141‑1 (MQ=255)
                             tACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGt                                                                                                      >  1:477923/1‑141 (MQ=255)
                               ccGCCTAATTTTTCAACTAATTTTATTGCTGCGTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCa                                                                                                    <  2:331728/141‑1 (MQ=255)
                                cGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTCGGTCaa                                                                                                   >  1:300537/1‑140 (MQ=255)
                                cGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCAt                                                                                                   >  1:425425/1‑141 (MQ=255)
                                   cTAATTTTTCAACTAATTTTATTGCTGCTTCAATCTTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACt                                                                                                >  2:649119/1‑141 (MQ=255)
                                   cTAATTTTTCAACTAATTTCATTGCTGCTTCAGTCCGACCACCAGTAGCTAATAAAACATCTGTAATTAACCCACGTTGACCGGGTTTAATTGCATCTTTGTACATTGATAAAACATTTGTACCATATTCTCGGTCATAcc                                                                                                >  2:649095/1‑140 (MQ=255)
                                             aaCTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAAt                                                                                      <  1:18522/141‑1 (MQ=255)
                                               cTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGCCCTGGTTTAATTGCCTCCTTCTCCCTTGTCAAGCCATATCTACAATATTCTAGGTCTCACTCTTATCGATTGa                                                                                    <  2:473138/141‑1 (MQ=255)
                                                 taatttATTGCTCCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACt                                                                                  <  2:500877/138‑1 (MQ=255)
                                                 taTTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACt                                                                                  <  2:499372/140‑1 (MQ=255)
                                                 aaTTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACt                                                                                  <  2:736606/141‑1 (MQ=255)
                                                      tATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTAATAACGAATGACTTCACg                                                                             >  2:249325/1‑141 (MQ=255)
                                                         tgctgtTTCACTCGTACCAGCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAgg                                                                          <  2:644262/141‑1 (MQ=255)
                                                         tgctgcTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAgg                                                                          <  1:439171/141‑1 (MQ=255)
                                                         tgctgcGTTCATCGCACCACCAGGAGCAAATAATTTATCTGTACTTAACACACGTTGACCTGGTTTAATTGCATCTTTTTACATTGTTAAAACATTTGTACCATATTCTAGTTCATACTCATAACGAATGACTTCACGAgg                                                                          <  2:526653/141‑1 (MQ=255)
                                                         tgcggcCTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAgg                                                                          <  1:765656/141‑1 (MQ=255)
                                                           ctgctTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTa                                                                        <  2:316733/141‑1 (MQ=255)
                                                           ctgctTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTa                                                                        <  2:132674/141‑1 (MQ=255)
                                                             gctTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAAt                                                                      >  1:610584/1‑141 (MQ=255)
                                                               ttCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTCATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAAttt                                                                    <  2:580002/141‑1 (MQ=255)
                                                               ttCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAAttt                                                                    <  1:437670/141‑1 (MQ=255)
                                                                tCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTc                                                                   <  1:707533/141‑1 (MQ=255)
                                                                tCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTc                                                                   <  1:261014/141‑1 (MQ=255)
                                                                tCAATCGTACCACCAGGAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTc                                                                   <  1:99863/141‑1 (MQ=255)
                                                                 cAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTcc                                                                  >  1:49965/1‑141 (MQ=255)
                                                                 cAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTcc                                                                  >  2:218987/1‑141 (MQ=255)
                                                                 cAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTcc                                                                  <  2:106276/141‑1 (MQ=255)
                                                                  aaTCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTccc                                                                 <  1:47893/141‑1 (MQ=255)
                                                                    tCGTACCACCGGTAGCTAATAAATCATCTGTAATTAACATACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCctt                                                               <  2:224451/141‑1 (MQ=255)
                                                                       ttcttccaGTATCTAATAAATCATCTGTAATTAACACTCGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCcttctt                                                            <  2:725389/136‑1 (MQ=255)
                                                                       tACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAATATTTGTACCATATTCTAGGTCATACTCATTACGAATGACTTCACGAGGTAATTTCCcttctt                                                            <  1:580319/141‑1 (MQ=255)
                                                                       tACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCcttctt                                                            <  1:517199/141‑1 (MQ=255)
                                                                        accaccAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCttt                                                           >  2:807638/1‑141 (MQ=255)
                                                                        accaccAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCttt                                                           >  1:32390/1‑141 (MQ=255)
                                                                          caccaGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTc                                                         <  1:639218/141‑1 (MQ=255)
                                                                              aGTAGCTAATAAATCATCGTTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAAc                                                     <  1:182060/141‑1 (MQ=255)
                                                                                    taataaATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGc                                               <  2:192709/141‑1 (MQ=255)
                                                                                        aaaTCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCaaaa                                           >  1:606138/1‑140 (MQ=255)
                                                                                           tcatcTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCa                                        <  1:184583/141‑1 (MQ=255)
                                                                                                       aaCACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGaa                            <  1:383669/141‑1 (MQ=255)
                                                                                                        acacacGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAACCCAATCCCCATTGAAt                           >  1:755345/1‑141 (MQ=255)
                                                                                                        acacacGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTCCTAACAGGTGCAAAGCCAATCCCCATTGAAt                           >  2:709239/1‑141 (MQ=255)
                                                                                                           cacGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTTTAACAAGTGCAAAGCCAATCCTCATTGAATAAg                        >  1:155532/1‑141 (MQ=255)
                                                                                                           cacGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAg                        >  1:326845/1‑141 (MQ=255)
                                                                                                            gcGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTCCTTTTCTAACAGGTGCAAAGCCAATCCCCAGTGAATAAGc                       <  1:541918/140‑1 (MQ=255)
                                                                                                                    cTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGAc               <  2:230266/141‑1 (MQ=255)
                                                                                                                     tGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTACCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCGTTGAATAAGCTACAGGACa              <  1:525291/141‑1 (MQ=255)
                                                                                                                             ttGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGa      >  2:413310/1‑141 (MQ=255)
                                                                                                                                 aTCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATaaa  <  1:218987/141‑1 (MQ=255)
                                                                                                                                 aTCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATaaa  <  2:49965/141‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                    
TTCAATTATAAATGCAATACCTACTACGATACCGCCTAATTTTTCAACTAATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAA  >  CP000730/1744225‑1744494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: