Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,026,958 C→T 5.2% G330D (GGT→GAT)  vraS ← sensor histidine kinase VraS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,026,9580CT 5.2% 330.2 / 5.9 107G330D (GGT→GAT) vraSsensor histidine kinase VraS
Reads supporting (aligned to +/- strand):  ref base C (56/45);  new base T (3/3);  total (59/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.08e-01

Only 100 of 108 total aligned reads displayed.

CCAACTACTTCAATATCACTTTGCGTTGATAGATAACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTATCTTGAATTCTCAACAATAA  >  CP000730/2026819‑2027097
                                                                                                                                           |                                                                                                                                            
ccAACTACTTCAATATCACTTTGCGTTGATAGATAACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTGTAcc                                                                                                                                             <  1:581255/141‑1 (MQ=255)
   actactTCAATATCACTTTGCGTTGATAGATAACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTGTATCTGa                                                                                                                                          >  1:640660/1‑141 (MQ=255)
    ctactTTAATGTCACTTTGCGTTGATAGATAACTTGAAATTCGTATACGGACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTTCTTATTTAAAGGTGCTTTCACCTCGATACGTGTACCTGaa                                                                                                                                         <  1:623574/141‑1 (MQ=255)
        ttCAATATCACTTTGCGTTGATAGATAACTTGAAATTCCTATACGTACTATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGCTTCTTTCACTTCGATACGTGTACCTGAATCTg                                                                                                                                     >  2:354099/1‑141 (MQ=255)
        ttCAATATCACTTTGCGTTGATAGATAACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTGTACCTGAATCTg                                                                                                                                     >  2:110785/1‑141 (MQ=255)
         tCAATATCACTTTGCGTTGATAGATAACTTGAGATTCCTATACGTACCATTTCATGCTCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTCTACCTGAATCttg                                                                                                                                    >  1:196938/1‑139 (MQ=255)
             tatCACTTTGCGTTGATAGATAACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGCAAt                                                                                                                                <  1:176637/141‑1 (MQ=255)
             tatCACTTGGCGTTGATAGATAACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGCAAt                                                                                                                                <  1:501940/141‑1 (MQ=255)
              atCACTTTGCGTTGATAGATAACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGCAATg                                                                                                                              <  2:440997/141‑1 (MQ=255)
                    ttGCGTTGATAGATAACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGCAATGATACaa                                                                                                                        >  2:581818/1‑141 (MQ=255)
                      gCGTTGATAGATAACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTCTTTAAAGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGCAATGATACAata                                                                                                                      >  1:629326/1‑141 (MQ=255)
                       cGTTGATAGATAACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATCCGTGTACCTGAATCTGGCAATGTTACAattt                                                                                                                     >  2:63635/1‑139 (MQ=255)
                        gTTGATAGATAACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATg                                                                                                                    <  1:37043/141‑1 (MQ=255)
                        gTTGATAGATAACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGCAATAATACAATATg                                                                                                                    >  2:465626/1‑141 (MQ=255)
                         ttGATAGATAACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGATGCTTTCACCTCGATACGTGTACCTGAATCTGGCACTGCTACAATATgg                                                                                                                   >  2:118857/1‑141 (MQ=255)
                            atagataACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGATGCTTTCACCTCGATACGTATACCTGAATCTCACAATGATACGATATTGAAc                                                                                                                >  1:83667/1‑141 (MQ=255)
                                 taACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGc                                                                                                           <  2:298893/141‑1 (MQ=255)
                                     ttaaaaTTCCTAGACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCa                                                                                                       <  2:758964/138‑1 (MQ=255)
                                     ttGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTGTACCTGAATCCGCACTCTGTAGAATATGGAACGTTGCACCa                                                                                                       <  1:595057/141‑1 (MQ=255)
                                         aaTTCCTATACGTACCATTTCATGATCATCCACAAACACTCCTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAAttt                                                                                                   >  2:531755/1‑141 (MQ=255)
                                         aaTTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAAttt                                                                                                   >  2:318744/1‑141 (MQ=255)
                                              cTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGCAATGATGCACTATGGAACGTTTCACCAATTTCCaaa                                                                                              >  1:720036/1‑141 (MQ=255)
                                                ttaCGGACCATTTCATGGTCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGc                                                                                            <  2:308410/140‑1 (MQ=255)
                                                ataCGTACCCTTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGAAATGATACAATATGGAACGTTGCACCAATTTCCAAAGc                                                                                            <  2:351831/141‑1 (MQ=255)
                                                ataCGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTGTACATGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGc                                                                                            >  1:596352/1‑141 (MQ=255)
                                                ataCGTACCATCTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGc                                                                                            <  2:262518/141‑1 (MQ=255)
                                                 taCGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGATCGTTGCACCAATTTCCAAAGct                                                                                           <  2:251031/141‑1 (MQ=255)
                                                  aCGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGctc                                                                                          <  2:529527/141‑1 (MQ=255)
                                                   cGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGctct                                                                                         <  1:706774/141‑1 (MQ=255)
                                                       ccATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCa                                                                                     <  2:250387/141‑1 (MQ=255)
                                                         aTTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGAAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACg                                                                                   <  2:739200/141‑1 (MQ=255)
                                                          tttCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTCAATGAAGGTGCTTTCACCTCGATACGTGTACCTGAATCGCGCAATGATACAAGATGGAACGTTGCACCAATTTCCAACGCTCTTTCActc                                                                                  >  1:672515/1‑139 (MQ=255)
                                                          ttccATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCCCCAGTTTCCAAAGGCCTTTCACGc                                                                                  >  2:137482/4‑141 (MQ=255)
                                                           ttCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCa                                                                                 >  2:700899/1‑141 (MQ=255)
                                                             cATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTGTATCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCata                                                                               <  1:569029/141‑1 (MQ=255)
                                                             cATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTGTATCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCata                                                                               <  1:219340/141‑1 (MQ=255)
                                                             cATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCata                                                                               <  1:254125/141‑1 (MQ=255)
                                                             cATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCata                                                                               <  1:126729/141‑1 (MQ=255)
                                                               tGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATAtt                                                                             <  2:784020/141‑1 (MQ=255)
                                                               tGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATAtt                                                                             <  1:505371/141‑1 (MQ=255)
                                                                gATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTGTATCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATAttt                                                                            >  2:387957/1‑141 (MQ=255)
                                                                     tcCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAg                                                                       >  2:444783/1‑141 (MQ=255)
                                                                       cacaAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACAAATTTCCACAGCTCTTTCACGCATATTTTTAAGTc                                                                     <  2:799839/141‑1 (MQ=255)
                                                                       cacaAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCCCGATACGTGTACCTGAATCTGGGAATGATACAATATGAAACGATGCACCAATTTCCAAAGATCTTTAACCAATCTTTTTCAGta                                                                     >  2:713336/1‑140 (MQ=255)
                                                                        acaaacaaTACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTcc                                                                    <  1:177866/141‑1 (MQ=255)
                                                                          aaacaaTACTTTAATCGTCATACGAATCCGCCTTATTTAAAGGTGCCTTCAGCTGGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCAt                                                                  <  2:83778/141‑1 (MQ=255)
                                                                            acaaTACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATaa                                                                <  1:131044/141‑1 (MQ=255)
                                                                            acaaTACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATaa                                                                <  2:613631/141‑1 (MQ=255)
                                                                                  cTTTAATCGGCCTACGACTCCTCCTTATTTAAAGTTGCTTTCCCCTCGATACGTGTACCAGAATCTGGCAATGTTACAATATCGAACGTTGCACCAATTTCCAGCGCTCTTTCACGCCAATTTTTCAGTCCATAACTTAGt                                                          >  2:419972/1‑141 (MQ=255)
                                                                                     tAATCGTCATACGCATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCATTTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCt                                                       <  2:523859/141‑1 (MQ=255)
                                                                                          gTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTGTATCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAAttt                                                  <  1:387957/141‑1 (MQ=255)
                                                                                           tCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAAtttt                                                 >  2:12490/1‑141 (MQ=255)
                                                                                           tCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTGTACCTGAATCTGACAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAAtttt                                                 >  2:21009/1‑141 (MQ=255)
                                                                                            cATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAGGTCCCTAACTTTGTTCTAtttttt                                                >  1:622160/1‑141 (MQ=255)
                                                                                                cGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTtcatc                                            <  1:396009/141‑1 (MQ=255)
                                                                                                     cctcctTATTTAAAGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTGTAATTTTTCGTCAACAt                                       >  1:96578/1‑141 (MQ=255)
                                                                                                      ctcctTATTTAAAGGTGCTATACACTCTATGCTTGTACCAGGCTCGGGCACCGTGACAGTATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACAtt                                      <  1:584276/141‑1 (MQ=255)
                                                                                                        cctTATTTAAAGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAGCTTTGCTCTAATTTTTCATCAACCTTaa                                    >  2:733082/1‑141 (MQ=255)
                                                                                                          ttATTTAAAGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATTCAAATTaaaa                                  >  2:184469/1‑141 (MQ=255)
                                                                                                          ttATTTAAAGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGACCATAACTTTGTTCTAATTTTTCATCAACATTaaaa                                  >  2:442114/1‑141 (MQ=255)
                                                                                                           tATTTAAAGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAAc                                 <  1:321380/141‑1 (MQ=255)
                                                                                                              ttAAAGGTGCTTTCACCTCGATACGTGTATCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCtt                              >  1:783736/1‑141 (MQ=255)
                                                                                                               tAACGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGCAATTATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCttt                             <  1:740115/141‑1 (MQ=255)
                                                                                                                  aGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGCACTGATACAATATGACAGGCTGCGCCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTAcc                          >  1:153568/1‑141 (MQ=255)
                                                                                                                  aGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTAcc                          >  1:16179/1‑141 (MQ=255)
                                                                                                                  aGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTAcc                          >  2:414502/1‑141 (MQ=255)
                                                                                                                  aGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTAcc                          >  1:703212/1‑141 (MQ=255)
                                                                                                                  aGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGGTCTAATTTTTCATCAACATTAAAACACTTAcc                          >  2:185371/1‑141 (MQ=255)
                                                                                                                    gTGCTTTCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCAt                        >  1:64255/1‑141 (MQ=255)
                                                                                                                       cTTTCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTAt                     >  1:322778/1‑141 (MQ=255)
                                                                                                                       cTTTCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTAt                     >  1:522251/1‑141 (MQ=255)
                                                                                                                       cTTTCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTAt                     >  1:798771/1‑141 (MQ=255)
                                                                                                                       cTTTCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTAt                     >  1:88122/1‑141 (MQ=255)
                                                                                                                        tttCACCTCGATACGTGTACCTGAATCTGGCAATGATCCAATAAGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTATc                    <  1:753291/141‑1 (MQ=255)
                                                                                                                        tttCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTATc                    <  1:207730/141‑1 (MQ=255)
                                                                                                                        tttCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTATc                    <  1:27785/141‑1 (MQ=255)
                                                                                                                        tttCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTATc                    <  1:693839/141‑1 (MQ=255)
                                                                                                                         ttCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTATCt                   >  2:469292/1‑141 (MQ=255)
                                                                                                                          tCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACCTTAAAACCTTTACCATTATCtt                  >  1:16281/1‑141 (MQ=255)
                                                                                                                          tCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTTTCtt                  >  2:272169/1‑141 (MQ=255)
                                                                                                                          tCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTATCtt                  >  2:34773/1‑141 (MQ=255)
                                                                                                                          tCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTATCtt                  >  2:467448/1‑141 (MQ=255)
                                                                                                                          tCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTATCtt                  >  1:13909/1‑141 (MQ=255)
                                                                                                                          tCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGGTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCGTCACCTTTAGCACTTTTACCATTATCtt                  >  1:16044/1‑141 (MQ=255)
                                                                                                                          tCACCTCGATACGTGTACCTGAATCTGACAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTATCtt                  >  2:710442/1‑141 (MQ=255)
                                                                                                                           cACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTATCTTg                 <  1:502150/141‑1 (MQ=255)
                                                                                                                            cccTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTATCTTGa                <  2:134164/140‑1 (MQ=255)
                                                                                                                            aCCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATTACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTCCCATTATCTTGa                >  2:80828/1‑141 (MQ=255)
                                                                                                                            aCCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTATCTTGa                >  2:461207/1‑141 (MQ=255)
                                                                                                                            aCCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTATCTTGa                >  2:80752/1‑141 (MQ=255)
                                                                                                                            aCCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTATCTTGa                >  2:272212/1‑141 (MQ=255)
                                                                                                                             ccTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTATCTTGaa               >  2:93012/1‑141 (MQ=255)
                                                                                                                             ccTCGATACGTGTACCTGAATCTGGAAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTCCCATTCTCTTGat               >  2:239126/1‑140 (MQ=255)
                                                                                                                             caacGATACGTGTACCTGAATCTGGCAATGCTACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTATCTTGaa               >  2:296596/4‑141 (MQ=255)
                                                                                                                               ccGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGGCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTATCTTGAAtt             <  1:110785/140‑1 (MQ=255)
                                                                                                                                 gATACGGGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTATCTTGAATtct           <  1:266495/141‑1 (MQ=255)
                                                                                                                                         tACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTATCTTGAATTCTCAACaata   <  1:63635/141‑1 (MQ=255)
                                                                                                                                          aCCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTATCTTGAATTCTCAACaataa  <  1:581818/141‑1 (MQ=255)
                                                                                                                                          aCCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTATCTTGAATTCTCAACaataa  >  2:42735/1‑141 (MQ=255)
                                                                                                                                          aCCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACCTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTATCTTGAATTCTCAACaataa  >  2:587986/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                            
CCAACTACTTCAATATCACTTTGCGTTGATAGATAACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGGAACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTATCTTGAATTCTCAACAATAA  >  CP000730/2026819‑2027097

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: