Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,399,409 C→G 4.8% E154Q (GAA→CAA)  USA300HOU_2263 ← acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,399,4090CG 4.8% 255.3 / 2.8 80E154Q (GAA→CAA) USA300HOU_2263acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (28/45);  new base G (2/2);  total (33/47)
Fisher's exact test for biased strand distribution p-value = 6.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTGGTGCCGAAATTGTTCTAATACACCAAATCCATCTAATTCAATGTAATTTTCACTTTCAATGACATCTACAACGCCAATAGGTTCATTATTTAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCATGTGCATCTGCATAGTCTTTTCCAAATGGCAAACTAAACTGATATGCAACTTTTAAATAGGCATCTATATGCGACTCGTCAACGATGGCAATTTCAACGTCAGAGTTACGCGGCAAATTGGCAA  >  CP000730/2399271‑2399549
                                                                                                                                          |                                                                                                                                            
cTTGATGCCGAAATTGTTCTAATACACCAAATCCATCTAATTCAATCTAATTTTCACTTTCAATGACATCTACAACGCCAATAGGTTCATTATTTAAATAAGCTACTAAGCGTTTAATCACATCTCTTTGATAATGTTccc                                                                                                                                            >  1:570100/1‑141 (MQ=255)
 ttGGTGCCGAAATTGTTCTAATACACCAAATCCATCTAATTCAATGTAATTTTCACTTTCAATGACATCTACAACGCCAATAGGTTCATTATTTAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCt                                                                                                                                           <  2:598696/141‑1 (MQ=255)
  tGGTGCCGAAATTGTTCTAATACACCAAATCCATCTAATTCAATGTAATTTTCACTTTCAATGACATCTACAACGCCAATAGGTTCATTATTTAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCtt                                                                                                                                          >  2:312520/1‑141 (MQ=255)
  tGGTGCCGAAATTGTTCTAATACACCAAATCCATCTAATTCAATGTAATTTTCACTTTCAATGACATCTACAACGCCAATAGGTTCATTATTTAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCtt                                                                                                                                          >  2:539757/1‑141 (MQ=255)
    gTGCCGAAATTGTTCTAATACACCAAATCCATCTAATTCAATGTAATTTTCACTTTCAATGACATCTACAACGCCAATAGGTTCATTATTTAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTAc                                                                                                                                        <  2:615358/141‑1 (MQ=255)
    gTGCCGAAATTGTTCTAATACACCAAATCCATCTAATTCAATGTAATTTTCACTTTCAATGACATCTACAACGCCAATAGGTTCATTATTTAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTAc                                                                                                                                        <  2:270226/141‑1 (MQ=255)
               gTTCTCACACACCAGATCCATCTAATTCTATGTACTTTTCACTTTCAATGACATCTACAACGCCAATAGGTTCATTATTTAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCAt                                                                                                                             <  2:809134/141‑1 (MQ=255)
               gTTCTAATACACCAAATCCATCTCATTCCATGTAATTTTCACTTTCATTGTCTTCTACAACGCATATAGGTTCATTATTTAAAGACGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCAt                                                                                                                             <  2:529045/141‑1 (MQ=255)
               gTTCTAATACACCAAATCCATCTAATTCAATTTATTTTTCACTTTCAATGACATCTACAACGCCAATAGGTTCATTATTCAACTAAGCTACTAAGCGTCTAACCACATCTTTTCGACCATGTTCCCTCACCTGTGCTTCAt                                                                                                                             <  2:392690/141‑1 (MQ=255)
               gTTCTAATACACCAAATCCATCTAATTCAATGTAATTTTCACTTTCAATGACATCTACAACGCCAATAGGTTCATTATTTAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCAt                                                                                                                             <  2:471405/141‑1 (MQ=255)
               gTTCTAATACACCAAATCCATCTAATTCAATGTAATTTTCACTTTCAATGACATCTACAACGCCAATAGGTTCATTATTTAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCAt                                                                                                                             <  2:451248/141‑1 (MQ=255)
                ttCTAATACACCAAATCCATCTAATTCAATGTAATTTTCACTTTCAATGACATCTACAACGCCAATAGGTTCATTATTTAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCAtg                                                                                                                            <  2:520652/141‑1 (MQ=255)
                       acacCAAATCCATCTAATTCAATGTAATTTTCACTTTCAATGACATCTACAACGCCAATAGGTTCATTATTTAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCATGTGCATCt                                                                                                                     <  2:46374/141‑1 (MQ=255)
                           cAAATCCATCTAATTCAATGTAATTTTCACCTTCAATGACATCAAACAAGCCAATAGGTTCATTATTTAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCATGTGCATCTGCAt                                                                                                                 <  1:383165/141‑1 (MQ=255)
                            aaaTCCATCTAATTCAATGTAATTTTCACTTTCAATGACATCTACAACGCCAATAGGTTCATTATTTAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCATGTGCATCTGCATa                                                                                                                <  1:163194/141‑1 (MQ=255)
                             aaTCCATCTATTTCAATGTAATTTTCACTTTCAATGACATCTACAACGCCAATCGGTTCATTATTTAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCATGTGCATCTGCATAg                                                                                                               >  2:274537/1‑141 (MQ=255)
                             aaTCCATCTAATTCAATGTAATTTTCACTTTCAATGACATCTACAACGCCAATAGGTTCATTATTTAAATAAGATACTAAGCGTTTAATCAAATCCTTTTGATAATGTCCCCTTACCATTTCTTCATGTGCATCTGCACAg                                                                                                               >  1:364413/1‑141 (MQ=255)
                               accATCTAATTCAATGTAATTTTCACTTTCAATGACATCTACAACGCCAATAGGTTCATTATTTAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCATGTGCATCTGCATAGTc                                                                                                             <  1:220748/140‑1 (MQ=255)
                                tcgtatcataaaaTGTAATTTTCACTTTCAATGACATCTACAACGCCAATAGGTTCATTATTTAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCATGTGCATCTGCATAGTCt                                                                                                            <  2:364256/130‑1 (MQ=255)
                                ccATCTAATTCAATGTAATTTTCACTTTCAATGACATCTACAACGCCAATAGGTTCATTATTTAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCATGTGCATCTGCATAGTCt                                                                                                            <  1:394694/141‑1 (MQ=255)
                                ccATCTAATTCAATGTAATTTTCACTTTCAATGACATCTACAACGCCAATAGGTTCATTATTTAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCATGTGCATCTGCATAGTCt                                                                                                            <  2:297954/141‑1 (MQ=255)
                                  aTCTAATTCAATGTAATTTTCACTTTCAATGACATCTACAACGCCAATAGGTTCATTATTTAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCATGTGCATCTGCATAGTCttt                                                                                                          >  1:364069/1‑141 (MQ=255)
                                        ttCAATGTAATTTTCACTTTCAATGACATCTACAACGCCAATAGGTTCATTATTTAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTGCCTTACCATTTCTTCATGTTCATCTGCATAGTCTTTTCCcaa                                                                                                    >  2:645272/1‑141 (MQ=255)
                                        ttCAATGTAATTTTCACTTTCAATGACATCTACAACGCCAATAGGTTCATTATTTAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTGCCTTACCATTTCTTCATGTGCATCTGCATAGTCTTTTCCaaa                                                                                                    >  2:101562/1‑141 (MQ=255)
                                            aTGTAATTTTCACTTTCAATGACATCTACAACGCCAATAGGTTCATTATTTAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCATGTGCATCTGCATAGTTTTTTTCCAATGGc                                                                                                >  2:150716/1‑141 (MQ=255)
                                            aTGTAATTTTCACTTTCAATGACATCTACAAAGCCAATAGGTTCATTATTTAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCATGTGCATCTGCATAGTCTTTTCCAACTgga                                                                                                >  2:411583/1‑140 (MQ=255)
                                               tAATTTTCACTTTCAATGACATCTACAACGCCAATAGGTTCATTATTTAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCATGTGCATCTGCATAGTCTTTTCCAAATGGCaaa                                                                                             <  2:400750/141‑1 (MQ=255)
                                                 aTTTTCACTTTCAATGACATCTACAACGCCAATAGGTTCATTATTTAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCATGTGCATCTGCATAGTCTTTTCCAAATGGCaaact                                                                                           >  1:313621/1‑141 (MQ=255)
                                                  ttttCACTTTCAATGACATATACAAAGCCAATAGGTTCATTATTCAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCATGTGCATCTGCATAGTCTTTTCCAAATGGCaaacta                                                                                          >  1:445119/1‑141 (MQ=255)
                                                   tttCACTTTCAATGACATCTACAACGCCAATAGGTTCATTATTTAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCATGTGCATCTGCATAGTCTTTTCCAAATGGCaaactaa                                                                                         >  2:211610/1‑141 (MQ=255)
                                                    ttCACTTTCAATGACATCTACAACGCCAATAGGTTCATTATTTAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCATGTGCATCTGCATAGTCTTTTCCAAATGGCaaactaaa                                                                                        <  2:21887/141‑1 (MQ=255)
                                                         tttCAATGACATCTACAACGCCAATAGGTTCATTATTTAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCATGTGCATCTGCATAGTCTTTTCCAAATGGCAAACTAAACTGat                                                                                   <  2:795755/141‑1 (MQ=255)
                                                            cAATGACATCTACAACGCCAATAGTTTCATTATTTAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCATGTGCATCTGCATAGTCTTTTCCAAATGGCAAACTAAACTGATATg                                                                                <  2:63280/141‑1 (MQ=255)
                                                            cAATGACATCTACAACGCCAATAGGTTCATTATTTAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCATGTGCCTCTGCATAGTCTTTTCCAAATGGCAAACTAAACTGATATg                                                                                <  2:405760/141‑1 (MQ=255)
                                                            cAATGACATCTACAACGCCAATAGGTTCATTATTTAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCATGTGCATCTGCATAGTCTTTTCCAAATGGCAAACTAAACTGATATg                                                                                <  2:795145/141‑1 (MQ=255)
                                                            agaTGACCTCTACAACGTTGATTTGTACATTATTGAAACAAGCTACTAAGCGTTTAATCACGTCTTTTTGATAATGTGCCCTGAGCATTTCTTCATGTGCATCTGCATAGTCTTTTCCAAATGGCAATCTACCCTGATATg                                                                                <  2:393827/139‑1 (MQ=255)
                                                             aaTGACATCTACAACGCCAATAGGTTCATTATTTAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCATGTGCATCTGCATAGTCTTTTCCAAATGGCAAACTAAACTGATATGc                                                                               >  1:535859/1‑141 (MQ=255)
                                                                gACATCTACAACTCCAATAGGTTCATTATTTAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCATGTGCATCTGCATAGTCTTTTCCAAATGGCAAACTAAACTGATATGCAAc                                                                            <  2:44255/141‑1 (MQ=255)
                                                                 ccATCTACTACGGCAATAGGTTCATTATTTAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCATGTGCATCTGCATAGTCTTTTCCAAATGGCAAACTAAACTGATATGCAACt                                                                           <  2:364069/140‑1 (MQ=255)
                                                                 aCATCTACAACGCCAATATGTTCATTATTTAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCATGTGCATCTGCATAGTCTTTTCCAAATGGCAAACTAAACTGATATGCAACt                                                                           <  2:250254/141‑1 (MQ=255)
                                                                     cTACAACGCCAATAGGTTCATTATTTAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCATGTGCATCTGCATAGTCTTTTCCAAATGGCAAACTAAACTGATATGCAACTTTTa                                                                       <  1:539757/141‑1 (MQ=255)
                                                                     cTACAACGCCAATAGGTTCATTATTTAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCATGTGCATCTGCATAGTCTTTTCCAAATGGCAAACTAAACTGATATGCAACTTTTa                                                                       <  1:312520/141‑1 (MQ=255)
                                                                     cTACAACGCCAATAGGTTCATTATTTAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCATGTGCATCTGCATAGTCTTTTCCAAATGGCAAACTAAACTGATATGCAACTTTTa                                                                       <  1:77630/141‑1 (MQ=255)
                                                                      tACAACGCCAATAGGTTCATTATTTAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCATGTGCATCTGCATAGTCTTTTCCAACTGTCAAACTAAACTGATATGCAACTTTTaa                                                                      <  2:473130/141‑1 (MQ=255)
                                                                      tACAACGCCAATAGGTTCATTATTTAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCATGTGCATCTGCATAGTCTTTTCCAAATGGCAAACTAAACTGATATGCAACTTTTaa                                                                      <  1:384278/141‑1 (MQ=255)
                                                                       aCAACGCCAATAGGTTCATTATTTAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCATGTGCATCTGCATAGTCTTTTCCAAATGGCAAACTAAACTGATATGCAACTTTTaaa                                                                     <  2:49573/141‑1 (MQ=255)
                                                                            gCCAATAGGTTCATTCTTTAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCATGTGCATCTGCATAGTCTTTTCCAAATGGCAAACTAAACTGATATGCAACTTTTAAATAGGc                                                                >  1:599567/1‑141 (MQ=255)
                                                                            gCCAATAGGTTCATTATTTAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTGCCTTACCATTTCTTCATGTGCATCTGCATAGTCTTTTCCAAATGGCAAACTAAACTGATATGCAACTTTTAAATAGGc                                                                <  1:101562/141‑1 (MQ=255)
                                                                            gCCAATAGGTTCATTATTTAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTGCCTTACCATTTCTTCATGTGCAGCTGCATAGTCTTTTCCAAATGGCAAACTAAACTGATATGCAACTTTTAAATAGGc                                                                <  1:645272/141‑1 (MQ=255)
                                                                             ccAACAGGTTCATTATTGAAATAAGCTACTAAGCGCTAACACACATCTTTTTGATAATGTTTCCTTACCATCTGTCCATGTGCATCTGCATAGTCTTTTCCAAATGGCAAACTACACTGATATGCACCTTTTAAAttgccg                                                               >  2:394472/1‑136 (MQ=255)
                                                                              cAATAGCTTCATTATTTAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCATGTGCATCTGCATAGTCTTTTCCAAATGGCAAACTAAACTGATATGCAACTTTTAAATCGGCAt                                                              >  2:95327/1‑141 (MQ=255)
                                                                               aaTAGGTTCATTATTTAAATAAGCTACTAAGCGTTTAATCACATCTTTTTTTTTTTTTTTTCTTACCATTTCTTCATGTGCATCTGCATATTCTTTTCCAGATGGCAACCTAAACTGATATGCAACTTTTAAATCGGCATc                                                             >  2:749555/1‑141 (MQ=255)
                                                                               aaTAGGTTCATTATTTAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCATGTGCATCTGCATAGTCTTTTCCAAATGGCAAACTAAACTGATATGCAACTTTTAAATAGGCATc                                                             >  1:147820/1‑141 (MQ=255)
                                                                               aaTAGGTTCATTATTTAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCATGTGCATCTGCATAGTCTTTTCCAAATGGCAAACTAAACTGATATGCAACTTTTAAATAGGCATc                                                             <  1:165204/141‑1 (MQ=255)
                                                                                 tAGGTTCATTATTTAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCATGTGCATCTGCATAGTCTTTTCCAAATGGCAAACTAAACTGATATGCAACTTTTAAATAGGCATCta                                                           >  1:2279/1‑141 (MQ=255)
                                                                                 tAGGTTCATTATTTAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCATGTGCATCTGCATAGTCTTTTCCAAATGGCAAACTAAACTGATATGCAACTTTTAAATAGGCATCta                                                           >  1:656346/1‑141 (MQ=255)
                                                                                  aGGTTCATTATTTAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCATGTGCATCTGCATAGTCTTTTCCAAATGGCAAACTAAACTGATATGCAACTTTTAAATAGGCATCtat                                                          <  1:655567/141‑1 (MQ=255)
                                                                                       cATTATTTAAATAAGCTCATATTCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCATGTGCATCTGCATAGTCTTTTCCAAATGGCAAACTAAACTGATATGCAACTTTTAAATAGGCATCTATATGCg                                                     >  2:779947/1‑141 (MQ=255)
                                                                                       cATTATTTAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCATGTGCATCTGCATAGTCTTTTCCAAATGGCAAACTAAACTGATATGCAACTTTTAAATAGGCATCTATATGCg                                                     >  2:741635/1‑141 (MQ=255)
                                                                                       cATTATTTAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCATGTGCATCTGCATAGTCTTTTCCAAATGGCAAACTAAACTGATATGCAACTTTTAAATAGGCATCTATATGCg                                                     >  1:126668/1‑141 (MQ=255)
                                                                                            tttAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCATGTGCATCTGCATAGTCTTTTCCAAATGGCAAACTAAACTGATATGCAACTTTTAAATAGGCATCTATATGCGACTCg                                                <  1:716185/141‑1 (MQ=255)
                                                                                            tttAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCATGTGCATCTGCATAGTCTTTTCCAAATGGCAAACTAAACTGATATGCAACTTTTAAATAGGCATCTATATGCGACTCg                                                <  1:762461/141‑1 (MQ=255)
                                                                                             ttAAATCAGCTACTAAGCGTTTAATCTCTTCTTTTTGATAATGTTCCCTTGCCATTTCTTCCTGTGCATCTGCCTAGTCTTTTCCAAATGGCAAACTAAACTGATATGCAACTTTTAAATAGGCATCTATATGCGCCTCGt                                               <  2:529856/141‑1 (MQ=255)
                                                                                                aaTAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCATGTGCATCTGCATAGTCTTTTCCAAATGGCAAACTCAACTGATATGCAACTTTTAAATAGGCATCTATATGCGACTCGTCaa                                            >  1:266140/1‑141 (MQ=255)
                                                                                                   aaGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCATGTGCATCTGCATAGTCTTTTCCAAATGGCAAACTAAACTGATATGCAACTTTTAAATAGGCATCTATATGCGACTCGTCAACGa                                         <  1:707451/141‑1 (MQ=255)
                                                                                                   aaGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCATGTGCATCTGCATAGTCTTTTCCAAATGGCAAACTAAACTGATATGCAACTTTTAAATAGGCATCTATATGCGACTCGTCAACGa                                         <  1:705040/141‑1 (MQ=255)
                                                                                                     gCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCATGTGCATCTGCATAGTCTTTTCCAAATGGCAAACTAAACTGATATGCAACTTTTAAATAGGCATCTATATGCGAGTCGACAACgcgt                                       >  1:346091/1‑138 (MQ=255)
                                                                                                     gCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCATGTGCATCTGCATAGTCTTTTCCAAATGGCAAACTAAACTGATATGCAACTTTTAAATAGGCATCTATATGCGACTCGTCAACGATg                                       >  1:659100/1‑141 (MQ=255)
                                                                                                     gCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCATGTGCATCTGCATAGTCTTTTCCAAATGGCAAACTAAACTGATATGCAACTTTTAAATAGGCATCTATATGCGACTCGTCAACGATg                                       >  1:674862/1‑141 (MQ=255)
                                                                                                     gCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCATGTGCATCTGCATAGTCTTTTCCAAATGGCAAACTAAACTGATATGCAACTATAAAATAGGCATCTATATGCGAATCGTCAACGATg                                       >  1:43236/1‑141 (MQ=255)
                                                                                                       tatgaaGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCCTTTCTTCTACCGGATGTGCATATTCTTTTCCACATGGCAAACTAAGCGGATATGCAACTTTTAAATAGGCATCTATATGCGACTCGTCATCGATGGc                                     <  2:570100/137‑1 (MQ=255)
                                                                                                       tactaAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCATGTGCATCTGCATAGTCTTTTCCAAATGGCAAACTAAACTGATATGCAACTTTTAAATAGGCATCTATATGCGACTCGTCAACGATGGc                                     <  2:599567/141‑1 (MQ=255)
                                                                                                              cGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCATGTGCATCTGCATAGTCTTTTCCAAATGGCAAACTAAACTGATATGCAACTTTTAAATAGGCATCTATATGCGACTCGTCAACGATGGCAATTTCa                              >  1:302352/1‑141 (MQ=255)
                                                                                                                   aaTCACATCTTTTTTTTTTTTTTTCCTTACCATTTCTTCATGTACATCTGCATAGTCTTTTCCAAATGGCAAACTAGACTGCTATGCAACTTTTAAATAGGCATCTATATGCGTTTTTTCAATGTTTGCAATTTCAACGTc                         >  2:76966/1‑141 (MQ=255)
                                                                                                                        caTCTTTTTGATAATGTTCCCTTACCATTTCTTCATGTGCATCTGCATAGTCTTTTCCAAATGGCAAACTAAACTGATATGCAACTTTTAAATAGGCATCTATATGCGACTCTTCAACGATGGCAATTTCAACGTCAGAGt                    >  2:742131/1‑141 (MQ=255)
                                                                                                                           cTTTTTGATAATGTTCCCTTACCATTTCTTCATGTGCATCTGCATAGTCTTTTCCAAATGGCAAACTAAACTGATATGCAACTTTTAAATAGGCATCTATATGCGACTCGTCAACGATGGCAATTTCAACGTCAGAGTTAc                 >  2:730625/1‑141 (MQ=255)
                                                                                                                              tttGATAATGTTCCCTTACCATTTCTTCATGTGCATCTGCATAGTCTTTTCCAAATGGCAAACTAAACTGATATGCAACTTTTAAATAGGCATCTATATGCGACTCGTCAACGATGGCAATTTCAACGTCAGAGTTAcgcg              >  1:806916/1‑141 (MQ=255)
                                                                                                                                   tagtGTTCCCGTACCATTTCTGCCGTGGCTTCATCATAGTCTTTTCCAAATGGCAAACTAAACGGATATGCAACTTTTAAATAGGCATCTATATGCTACTCGTCAACGAGGTCAATTTCAACTTCAGAGTTACGCGGCaaa         <  2:364413/138‑1 (MQ=255)
                                                                                                                                   tAATGTTCCCTTACCATTTCTTCATGTGCATCTGCATAGTCTTTTCCAAATGGCAAACTAAACTGATATGCAACTTTTAAATAGGCATCTATATGCGACTCGTCAACGATGGCAATTTCAACGTCAGAGTTACGCGGCaaa         <  1:633501/141‑1 (MQ=255)
                                                                                                                                   tAATGTTCCCTTACCATTTCTTCATGTGCATCTGCATAGTCTTTTCCAAATGGCAAACTAAACTGATATGCAACTTTTAAATAGGCATCTATATGCGACTCGTCAACGATGGCAATTTCAACGTCAGAGTTACGCGGCaaa         <  1:274537/141‑1 (MQ=255)
                                                                                                                                   tAATGTTCCCTTACCATTTCTTCATGTGCATCTACATAGTCTTTTCCAAATGGCAAACTAAACTGATATGCAACTTTTAAATAGGCATCTATATGCGACTCGTCAACGATGGCAATTTCAACGTCAGAGTTACGCGGCaaa         <  2:384056/141‑1 (MQ=255)
                                                                                                                                          ccccTACCATTTCTTCATGTGCATCTGCATAGTCTTTTCCAAATGGCAAACTAAACTGATATGCAACTTTTAAATAGGCATCTATATGCGACTCGTCAACGATGGCAATTTCAACGTCAGAGTTACGCGGCAAATTGGCaa  <  1:675810/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                            
CTTGGTGCCGAAATTGTTCTAATACACCAAATCCATCTAATTCAATGTAATTTTCACTTTCAATGACATCTACAACGCCAATAGGTTCATTATTTAAATAAGCTACTAAGCGTTTAATCACATCTTTTTGATAATGTTCCCTTACCATTTCTTCATGTGCATCTGCATAGTCTTTTCCAAATGGCAAACTAAACTGATATGCAACTTTTAAATAGGCATCTATATGCGACTCGTCAACGATGGCAATTTCAACGTCAGAGTTACGCGGCAAATTGGCAA  >  CP000730/2399271‑2399549

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑